請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/4451
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 蔡志偉 | |
dc.contributor.author | Chien-Hao Tseng | en |
dc.contributor.author | 曾建豪 | zh_TW |
dc.date.accessioned | 2021-05-14T17:42:20Z | - |
dc.date.available | 2020-08-19 | |
dc.date.available | 2021-05-14T17:42:20Z | - |
dc.date.copyright | 2015-08-19 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-08-18 | |
dc.identifier.citation | Azzam O, Frazer J, De La Rosa D, Beaver JS, Ahlquist P, Maxwell DP. 1994. Whitefly transmission and efficient ssDNA accumulation of Bean golden mosaic geminivirus require functional coat protein. Virology 204: 289-296.
Briddon RW, Pinner MS, Stanley J, Markham PG. 1990. Geminivirus coat protein gene replacement alters insect specificity. Virology 177: 85-94. Brown JK, Czosnek H. 2002. Whitefly transmitted viruses. Adv Bot Res 36: 65-100. Brown JK, Fauquet CM, Briddon RW, Zerbini M, Moriones E, Navas-Castillo J. 2012. Geminiviridae. pp 351-373. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds). Virus taxonomy-Ninth report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, London. Caciagli P, Medina Piles VM, Marian D, Vecchiati M, Masenga V, Mason G, Falcioni T, Noris E. 2009. Virion stability is important for the circulative transmission of Tomato yellow leaf curl Sardinia virus by Bemisia tabaci, but virion access to salivary glands does not guarantee transmissibility. J Virol 83: 5784-5795. Czosnek H. 2008. Tomato yellow leaf curl virus (Geminiviridae). pp 138-145. In: Mahy BWJ, Van Regenmortel MHV. (eds). Encyclopedia of Virology, vol 5, 3rd ed. Elsevier, Oxford. Czosnek H, Ghanim M. 2011. Bemisia tabaci - Tomato yellow leaf curl virus interaction causing worldwide epidemics. pp 51-67. In: Thompson WMO (ed). The whitefly, Bemisia tabaci (Homoptera: Aleyrodidae) interaction with Geminivirus-infected host plants. Springer, Dordrecht. Czosnek H, Ghanim M, Ghanim M. 2002. The circulative pathway of begomoviruses in the whitefly vector Bemisia tabaci—insights from studies with Tomato yellow leaf curl virus. Ann Appl Biol 140: 215-231. Dinsdale A, Cook L, Riginos C, Buckley YM, De Barro P. 2010. Refined global analysis of Bemisia tabaci (Gennadius) (Hemiptera: Sternorrhyncha: Aleyrodoidea) mitochondrial cytochrome oxidase 1 to identify species level genetic boundaries. Ann Entomol Soc Am 103: 196-208. Fondong VN. 2013. Geminivirus protein structure and function. Mol Plant Pathol 14: 635-649. Gafni Y, Epel BL. 2002. The role of host and viral proteins in intra-and inter-cellular trafficking of geminiviruses. Physiol Mol Plant Pathol 60: 231-241. Gottlieb Y, Ghanim M, Gueguen G, Kontsedalov S, Vavre F, Fleury F, Zchori-Fein E. 2008. Inherited intracellular ecosystem: symbiotic bacteria share bacteriocytes in whiteflies. FASEB J 22: 2591-2599. Gottlieb Y, Zchori-Fein E, Mozes-Daube N, Kontsedalov S, Skaljac M, Brumin M, Sobol I, Czosnek H, Vavre F, Fleury F, Ghanim M. 2010. The transmission efficiency of Tomato yellow leaf curl virus by the whitefly Bemisia tabaci is correlated with the presence of a specific symbiotic bacterium species. J Virol 84: 9310-9317. Ghanim M. 2014. A review of the mechanisms and components that determine the transmission efficiency of Tomato yellow leaf curl virus (Geminiviridae; Begomovirus) by its whitefly vector. Virus Res 186: 47-54. Ghanim M, Medina V. 2007. Localization of Tomato yellow leaf curl virus in its whitefly vector Bemisia tabaci. pp 171-183. In: Czosnek H (ed). Tomato yellow leaf curl virus disease: Management, molecular Biology, breeding for Resistance. Springer, Dordrecht. Ghanim M, Morin S, Zeidan M, Czosnek H. 1998. Evidence for transovarial transmission of Tomato yellow leaf curl virus by its vector the whitefly Bemisia tabaci. Virology 240: 295-303. Ghanim M, Morin S, Czosnek H. 2001a. Rate of Tomato yellow leaf curl virus translocation in the circulative transmission pathway of its vector, the whitefly Bemisia tabaci. Phytopathology 91: 188-196. Ghanim M, Rosell RC, Campbell LR, Czosnek H, Brown JK, Ullman DE. 2001b. Microscopic analysis of the digestive, salivary and reproductive organs of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) biotype B. J Morphol 248: 22-40. Ghanim M, Brumin M, Popovski S. 2009. A simple, rapid and inexpensive method for localization of Tomato yellow leaf curl virus and Potato leafroll virus in plant and insect vectors. J Virol Methods 159: 311-314. Götz M, Popovski S, Kollenberg M, Gorovitz R, Brown JK, Cicero JM, Czosnek H, Winter S, Ghanim M. 2012. Implication of Bemisia tabaci heat shock protein 70 in begomovirus–whitefly interactions. J Virol 86: 13241-13252. Höfer P, Bedford ID, Markham PG, Jeske H, Frischmuth T. 1997. Coat protein gene replacement results in whitefly transmission of an insect nontransmissible geminivirus isolate. Virology 236: 288-295. Hogenhout SA, Ammar ED, Whitfield AE, Redinbaugh MG. 2008. Insect-vector interactions with persistently transmitted viruses. Annu Rev Phytopathol 46: 327-359. Höhnle M, Höfer P, Bedford ID, Briddon RW, Markham PG, Frischmuth T. 2001. Exchange of three amino acids in the coat protein results in efficient whitefly transmission of a nontransmissible Abutilon mosaic virus isolate. Virology 290: 164-171. Hu J, De Barro P, Zhao H, Nardi F, Wang J, Liu SS. 2011. An extensive field survey combined with a phylogenetic analysis reveals rapid and widespread invasion of two alien whiteflies in China. PLoS One 6: e16061. Ko CC, Hung YC, Wang CH. 2007. Sequence characterized amplified region markers for identifying biotypes of Bemisia tabaci (Hem., Aleyrodidae). J Appl Entomol 131: 542-547. Lazarowitz SG, Beachy RN. 1999. Viral movement proteins as probes for intracellular and intercellular trafficking in plants. Plant Cell 11: 535-548. Li CY, Cox-Foster D, Gray SM, Gildow F. 2001. Vector specificity of Barley yellow dwarf virus (BYDV) transmission: Identification of potential cellular receptors binding BYDV-MAV in the aphid, Sitobion avenae. Virology 286: 125-133. Li R, Xie W, Wang S, Wu Q, Yang N, Yang X, Pan H, Zhou X, Bai L, Xu B, Zhou X, Zhang Y. 2013. Reference gene selection for qRT-PCR analysis in the sweet potato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae). PLoS ONE 8: e53006. Morin S, Ghanim M, Zeidan M, Czosnek H, Verbeek M, van den Heuvel JFJM. 1999. A GroEL homologue from endosymbiotic bacteria of the whitefly Bemisia tabaci is implicated in the circulative transmission of Tomato yellow leaf curl virus. Virology 256: 75-84. Morin S, Ghanim M, Sobol I, Czosnek H. 2000. The GroEL protein of the whitefly Bemisia tabaci interacts with the coat protein of transmissible and nontransmissible begomoviruses in the yeast two-hybrid system. Virology 276: 404-416. Navas-Castillo J, Fiallo-Olivé E, Sánchez-Campos S. 2011. Emerging virus diseases transmitted by whiteflies. Annu Rev Phytopathol 49: 219-248. Noris E, Vaira AM, Caciagli P, Masenga V, Gronenborn B, Accotto GP. 1998. Amino acids in the capsid protein of Tomato yellow leaf curl virus that are crucial for systemic infection, particle formation, and insect transmission. J Virol 72: 10050-10057. Ohnishi J, Kitamura T, Terami F, Honda K. 2009. A selective barrier in the midgut epithelial cell membrane of the nonvector whitefly Trialeurodes vaporariorum to Tomato yellow leaf curl virus uptake. J Gen Plant Pathol 75: 131-139. Polston JE, De Barro P, Boykin LM. 2014. Transmission specificities of plant viruses with the newly identified species of the Bemisia tabaci species complex. Pest Manag Sci 70: 1547-1552. Tsai WS, Shih SL, Kenyon L, Green SK, Jan FJ. 2011. Temporal distribution and pathogenicity of the predominant tomato-infecting begomoviruses in Taiwan. Plant Pathol 60: 787-799. Wang LL, Wei XM, Ye XD, Xu HX, Zhou XP, Liu SS, Wang XW. 2014. Expression and functional characterisation of a soluble form of Tomato yellow leaf curl virus coat protein. Pest Manag Sci 70: 1624-1631. Wartig L, Kheyr-Pour A, Noris E, De Kouchkovsky F, Jouanneau F, Gronenborn B, Jupin I. 1997. Genetic analysis of the monopartite Tomato yellow leaf curl geminivirus: roles of V1, V2, and C2 ORFs in viral pathogenesis. Virology 228: 132-140. Watanabe S, Bressan A. 2013. Tropism, compartmentalization and retention of banana bunchy top virus (Nanoviridae) in the aphid vector Pentalonia nigronervosa. J Gen Virol 94: 209-219. Wei J, Zhao JJ, Zhang T, Li FF, Ghanim M, Zhou XP, Ye GY, Liu SS, Wang XW. 2014. Specific cells in the primary salivary glands of the whitefly Bemisia tabaci control retention and transmission of begomoviruses. J Virol 88: 13460-13468. Weng SH, Tsai WS, Kenyon L, Tsai CW. 2015. Different transmission efficiencies may drive displacement of tomato begomoviruses in the fields in Taiwan. Ann Appl Biol 166: 321-330. Whitfield AE, Kumar NKK, Rotenberg D, Ullman DE, Wyman EA, Zietlow C, Willis DK, German TL. 2008. A soluble form of the Tomato spotted wilt virus (TSWV) glycoprotein GN (GN-S) inhibits transmission of TSWV by Frankliniella occidentalis. Phytopathology 98: 45-50. Yaakov N, Levy Y, Belausov E, Gaba V, Lapidot M, Gafni Y. 2011. Effect of a single amino acid substitution in the NLS domain of Tomato yellow leaf curl virus-Israel (TYLCV-IL) capsid protein (CP) on its activity and on the virus life cycle. Virus Res 158: 8-11. Zhou G, Lu X, Lu H, Lei J, Chen S, Gong Z. 1999. Rice ragged stunt oryzavirus: role of the viral spike protein in transmission by the insect vector. Ann Appl Biol 135: 573-578. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/4451 | - |
dc.description.abstract | 粉蝨不僅藉由取食造成植株的危害,更做為媒介昆蟲傳播植物病毒。菸草粉蝨傳播的 begomoviruses 威脅世界上許多作物的栽培。番茄黃化捲葉泰國病毒 (Tomato yellow leaf curl Thailand virus, TYLCTHV) 及番茄捲葉台灣病毒 (Tomato leaf curl Taiwan virus, ToLCTWV) 是臺灣番茄田間最常見的兩種病毒。鞘蛋白 (coat protein, CP) 是 begomoviruses 唯一的結構蛋白,CP 被推測與菸草粉蝨中腸推定的受體 (receptor) 有交互作用。In vivo binding assay 結果顯示 recombinant TYLCTHV CP與菸草粉蝨 (Bemisia tabaci) 的中腸細胞有專一性的結合。本研究的目的是探討菸草粉蝨獲得病毒前先餵食 recombinant TYLCTHV CP 是否會競爭粉蝨中腸推定的受體,進而降低其獲得 TYLCTHV 病毒量與粉蝨傳播TYLCTHV的能力。實驗結果顯示先餵食 recombinant TYLCTHV CP 會降低 TYLCTHV 在粉蝨中腸細胞內的累積程度,但是卻沒有降低粉蝨體內的 TYLCTHV 病毒量。此外,先餵食 recombinant TYLCTHV CP會抑制粉蝨傳播TYLCTHV。本研究也利用相似的實驗來探討先餵食 recombinant TYLCTHV CP (homologous to ToLCTWV CP) 是否也會降低粉蝨中腸細胞內 ToLCTWV 的累積程度與粉蝨體內的病毒量,以及抑制粉蝨傳播 ToLCTWV。實驗結果指出先餵食 recombinant TYLCTHV CP不會降低 ToLCTWV 在菸草粉蝨中腸細胞內的累積程度與粉蝨體內的 ToLCTWV 病毒量。此外,先餵食 recombinant TYLCTHV CP也不會抑制菸草粉蝨傳播ToLCTWV。本研究證實 TYLCTHV CP 做為 attachment protein的功能並且能抑制菸草粉蝨傳播 TYLCTHV。此外,餵食 recombinant TYLCTHV CP 無法有效降低菸草粉蝨傳播 ToLCTWV 的結果也顯示 TYLCTHV CP與菸草粉蝨中腸的 receptor 具有專一性。了解 CP 與菸草粉蝨中腸的交互作用不僅可以揭開菸草粉蝨傳播 begomoviruses 的機制,日後進一步的研究更能幫助我們發展對抗病害的防治策略。 | zh_TW |
dc.description.abstract | Whiteflies not only damage plants through feeding but also serve as vectors transmitting plant viruses. Whitefly-transmitted begomoviruses threaten a wide range of crops worldwide. Tomato yellow leaf curl Thailand virus (TYLCTHV) and Tomato leaf curl Taiwan virus (ToLCTWV) are two most prevalent viruses in tomato fields in Taiwan. Coat protein (CP), the only structural protein of begomoviruses, is hypothesized to interact with a putative receptor in the midgut of Bemisia tabaci. In vivo binding assay demonstrated recombinant TYLCTHV CP specifically bound to the midgut cells of B. tabaci. The objective of this study was to determine if preacquisition of TYLCTHV CP competes the putative receptor in the midgut of B. tabaci hence suppresses the acquisition and vector transmission of TYLCTHV. Preacquisition of recombinant TYLCTHV CP decreased the accumulation level of TYLCTHV in the midgut cells of B. tabaci but did not reduce the acquisition of TYLCTHV in B. tabaci. Further, preacquisition of recombinant TYLCTHV CP suppressed the whitefly transmission of TYLCTHV. Similar experiments were conducted to investigate if preacquisition of TYLCTHV CP (homologous to ToLCTWV CP) would reduce virus accumulation and acquisition in B. tabaci, and the whitefly transmission of ToLCTWV. The results demonstrated that preacquisition of recombinant TYLCTHV CP did not influence the accumulation and acquisition of ToLCTWV in B. tabaci and did neither reduce the whitefly transmission of ToLCTWV. In conclusion, TYLCTHV CP functioned as an attachment protein and preacquisition of TYLCTHV CP reduced the whitefly transmission of TYLCTHV. The failure that using TYLCTHV CP to inhibit the whitefly transmission of ToLCTWV suggested that the interaction between TYLCTHV CP and the putative receptor in the midgut of B. tabaci is specific. Understanding the CP-midgut interaction would unveil the mechanism of whitefly transmission of begomoviruses and warrant further study to develop strategies for disease control. | en |
dc.description.provenance | Made available in DSpace on 2021-05-14T17:42:20Z (GMT). No. of bitstreams: 1 ntu-104-R02632005-1.pdf: 2686602 bytes, checksum: b3bc74310e9c2763900ed7c8674b9791 (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 誌謝 ii
中文摘要 iii ABSTRACT iv CONTENTS vi LIST OF FIGURES viii Chapter 1 Introduction 1 Chapter 2 Materials and methods 4 2.1 Insects, viruses, and plants 4 2.2 Construction of expression plasmids of viral proteins 4 2.3 Expression and purification of recombinant proteins 5 2.4 SDS-PAGE 6 2.5 In vivo binding assay 6 2.6 Competition for the virus binding site in midguts 7 2.7 Inhibition of TYLCTHV acquisition 8 2.8 Inhibition of TYLCTHV transmission 9 2.9 Inhibition of ToLCTWV acquisition and transmission 9 2.10 Statistics 11 Chapter 3 Results 12 3.1 Expression and purification of recombinant proteins 12 3.2 TYLCTHV CP bound to the midgut cells of B. tabaci 12 3.3 TYLCTHV CP competed with TYLCTHV for the midgut binding site in B. tabaci 13 3.4 Influence of TYLCTHV CP on the acquisition of TYLCTHV by B. tabaci 14 3.5 Influence of TYLCTHV CP on whitefly transmission of TYLCTHV 14 3.6 TYLCTHV CP did not compete with ToLCTWV for the midgut binding site in B. tabaci 15 3.7 Influence of TYLCTHV CP on the acquisition of ToLCTWV by B. tabaci 16 3.8 Influence of TYLCTHV CP on whitefly transmission of ToLCTWV 16 3.9 Inhibition efficiency of TYLCTHV CP on whitefly transmission of TYLCTHV and ToLCTWV 17 Chapter 4 Discussion 18 REFERENCES 22 | |
dc.language.iso | en | |
dc.title | 重組鞘蛋白抑制粉蝨傳播番茄黃化捲葉泰國病毒 | zh_TW |
dc.title | Recombinant Coat Protein Suppress Whitefly Transmission of Tomato yellow leaf curl Thailand virus | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張雅君,吳岳隆,黃莉欣,陳宗祺 | |
dc.subject.keyword | begomoviruses,菸草粉蝨,重組病毒蛋白,病媒傳播,免疫螢光分析, | zh_TW |
dc.subject.keyword | begomoviruses,Bemisia tabaci,recombinant viral protein,vector transmission,immunofluorescence assay, | en |
dc.relation.page | 36 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2015-08-18 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 昆蟲學研究所 | zh_TW |
顯示於系所單位: | 昆蟲學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-104-1.pdf | 2.62 MB | Adobe PDF | 檢視/開啟 |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。