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標題: | 利用半學生氏t檢定偵測異質性疾病基因表現差異 Detecting Differentially Expressed Genes in Heterogeneous Disease Using Half Student t test |
作者: | Chun-Lun Hsu 許郡倫 |
指導教授: | 李文宗 |
關鍵字: | 學生氏t檢定,基因表現,異質性疾病,檢力, Student t-test,gene-expression,heterogeneous disease,power, |
出版年 : | 2009 |
學位: | 碩士 |
摘要: | 基因表現研究為近年來熱門的研究題材。研究者常利用學生氏t檢定篩選出與疾病相關的基因。然而,當研究者所研究的疾病為異質性疾病時,病例組和對照組間基因表現平均值的差距可能不大,因而較難被傳統的學生氏t檢定偵測出來。本研究提出半學生氏t檢定(half Student t test)處理異質性疾病的情況。半學生氏t檢定的檢定統計量只考慮對照組的樣本標準差,而不考慮病例組的樣本標準差。作者以蒙地卡羅模擬及一個結腸腫瘤基因表現真實資料,比較半學生氏t檢定與傳統學生氏t檢定的統計檢力表現。在本研究模擬的情境下,半學生氏t檢定最多可比傳統學生氏t檢定多出約35%的統計檢力。另外,結腸腫瘤基因表現資料經過錯誤發現率(切點訂為0.05)的控制,半學生氏t檢定可比傳統學生氏t檢定多偵測出279個顯著基因。本研究所提出的半學生氏t檢定執行容易且統計檢力表現良好,值得推荐做為針對異質性疾病偵測基因表現差異的方法。 Gene-expression has been a popular research topic in recent years. Student t-test is commonly adopted to screen disease-related genes. However, when the researches are focused on heterogeneous disease, the means of gene-expression levels between case group and control group may be similar, and thus, the difference would be difficult to be detected by conventional Student t-test. This study proposed half Student t-test to examine heterogeneous disease. Test statistics of half Student t-test only considers sample standard deviation of control group, without considering the sample standard deviation of case group. This study applied Monte Carlo simulation and real gene-expression data of colon cancer to compare the power performance of half Student t-test and conventional Student t-test. Under the simulated scenario, this study found that half Student t-test could have 35% higher statistical power than conventional Student t-test. In addition, after false discovery rate (cut-off point set at 0.05) control of colon cancer gene-expression data, half Student t-test could detect 279 more significant genes than conventional Student t-test. Half Student t-test is easy to execute with good statistical power, and is worth to be recommended as a method of detecting heterogeneous disease gene-expression difference. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44389 |
全文授權: | 有償授權 |
顯示於系所單位: | 流行病學與預防醫學研究所 |
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