請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44140
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 張百恩 | |
dc.contributor.author | Wen-Yu Chen | en |
dc.contributor.author | 陳文裕 | zh_TW |
dc.date.accessioned | 2021-06-15T02:41:44Z | - |
dc.date.available | 2012-09-15 | |
dc.date.copyright | 2009-09-15 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-08-11 | |
dc.identifier.citation | Barron, M. J., McDonnell, S. T., Mackie, I., Dixon, M. J., 2008. Hereditary dentine disorders: dentinogenesis imperfecta and dentine dysplasia. Orphanet J Rare Dis. 3, 31.
Bellahcene, A., Kroll, M., Liebens, F., Castronovo, V., 1996. Bone sialoprotein expression in primary human breast cancer is associated with bone metastases development. Journal of bone and mineral research. 11, 665-670. Bellahcene, A., Merville, M., Castronovo, V., 1994. Expression of bone sialoprotein, a bone matrix protein, in human breast cancer. Cancer Research. 54, 2823-2826. Bhandari, S., Pannu, K., 2008. Dentinogenesis imperfecta: a review and case report of a family over four generations. Indian J Dent Res. 19, 357-61. Bianco, P., Fisher, L., Young, M., Termine, J., Robey, P., 1991. Expression of bone sialoprotein (BSP) in developing human tissues. Calcified Tissue International. 49, 421-426. Butler, W., 1998. Dentin matrix proteins. European Journal of Oral Sciences. 106, 204-210. Butler, W., Bhown, M., Dimuzio, M., Linde, A., 1981. Nonocollagenous proteins of dentin. Isolation and partial characterization of rat dentin proteins and proteoglycans using a three-step preparative method. Collagen and related research. 1, 187-199. Butler, W., Brunn, J., Qin, C., McKee, M., 2002. Extracellular matrix proteins and the dynamics of dentin formation. Connective Tissue Research. 43, 301-307. Canadillas, S., Rodriguez-Benot, A., Rodriguez, M., 2007. More on the bone-kidney axis--lessons from hypophosphataemia. Nephrol Dial Transplant. 22, 1521-3. Cate, A., 1998. Oral histology: development, structure and function. St. Louis: Mosby-Year Book. 18–23. Chai, Y., Jiang, X., Ito, Y., Bringas Jr, P., Han, J., Rowitch, D., Soriano, P., McMahon, A., Sucov, H., 2000. Fate of the mammalian cranial neural crest during tooth and mandibular morphogenesis. Development. 127, 1671-1679. Chaplet, M., De Leval, L., Waltregny, D., Detry, C., Fornaciari, G., Bevilacqua, G., Fisher, L., Castronovo, V., Bellahcene, A., 2003. Dentin matrix protein 1 is expressed in human lung cancer. Journal of Bone and Mineral Research. 18, 1506-1512. Cobourne, M., Sharpe, P., 2002. Expression and regulation of hedgehog-interacting protein during early tooth development. Connective Tissue Research. 43, 143-147. Cohn, S. A., 1957. Development of the molar teeth in the albino mouse. Am J Anat. 101, 295-319. D'Souza, R., Cavender, A., Sunavala, G., Alvarez, J., Ohshima, T., Kulkarni, A., MacDougall, M., 1997. Gene expression patterns of murine Dentin Matrix Protein 1 (Dmp1) and Dentin Sialophosphoprotein (DSPP) suggest distinct developmental functions in vivo*. Journal of Bone and Mineral Research. 12, 2040-2049. Du, S., Frenkel, V., Kindschi, G., Zohar, Y., 2001. Visualizing normal and defective bone development in zebrafish embryos using the fluorescent chromophore calcein. Developmental Biology. 238, 239-246. Fedarko, N., Fohr, B., Robey, P., Young, M., Fisher, L., 2000. Factor H binding to bone sialoprotein and osteopontin enables tumor cell evasion of complement-mediated attack. Journal of Biological Chemistry. 275, 16666-16672. Feng, J., Huang, H., Lu, Y., Ye, L., Xie, Y., Tsutsui, T., Kunieda, T., Castranio, T., Scott, G., Bonewald, L., 2003. The Dentin matrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development. Journal of dental research. 82, 776-780. Feng, J., Luan, X., Li, X., Unterbrink, A., Harris, S., MacDougall, M., 1998a. Mouse dentin matrix protein 1 gene: Analysis of genomic structure and promoter activity in odontoblasts and osteoblasts. American Academy of Orthopaedic Surgeons. 1, 155-159. Feng, J., Luan, X., Wallace, J., Jing, D., Ohshima, T., Kulkarni, A., D'Souza, R., Kozak, C., MacDougall, M., 1998b. Genomic organization, chromosomal mapping, and promoter analysis of the mouse dentin sialophosphoprotein (Dspp) gene, which codes for both dentin sialoprotein and dentin phosphoprotein. Journal of Biological Chemistry. 273, 9457-9464. Feng, J., Zhang, J., Dallas, S., Lu, Y., Chen, S., Tan, X., Owen, M., Harris, S., MacDougall, M., 2002. Dentin matrix protein 1, a target molecule for Cbfa1 in bone, is a unique bone marker gene. Journal of Bone and Mineral Research. 17, 1822-1831. Feng, J. Q., Ward, L. M., Liu, S., Lu, Y., Xie, Y., Yuan, B., Yu, X., Rauch, F., Davis, S. I., Zhang, S., Rios, H., Drezner, M. K., Quarles, L. D., Bonewald, L. F., White, K. E., 2006. Loss of DMP1 causes rickets and osteomalacia and identifies a role for osteocytes in mineral metabolism. Nat Genet. 38, 1310-5. Fisher, L., Jain, A., Tayback, M., Fedarko, N., 2004. Small integrin binding ligand N-linked glycoprotein gene family expression in different cancers. Clinical cancer research. 10, 8501-8511. Fisher, L., Torchia, D., Fohr, B., Young, M., Fedarko, N., 2001. Flexible structures of SIBLING proteins, bone sialoprotein, and osteopontin. Biochemical and Biophysical Research Communications. 280, 460-465. Fleming, A., Keynes, R., Tannahill, D., 2004. A central role for the notochord in vertebral patterning. Development. 131, 873-880. Ganss, B., Kim, R., Sodek, J., 1999. Bone sialoprotein. Critical Reviews in Oral Biology & Medicine. 10, 79-98. George, A., Gui, J., Jenkins, N., Gilbert, D., Copeland, N., Veis, A., 1994. In situ localization and chromosomal mapping of the AG1 (Dmp1) gene. Journal of Histochemistry and Cytochemistry. 42, 1527-1531. George, A., Sabsay, B., Simonian, P., Veis, A., 1993. Characterization of a novel dentin matrix acidic phosphoprotein. Implications for induction of biomineralization. Journal of Biological Chemistry. 268, 12624-12630. George, A., Silberstein, R., Veis, A., 1995. In situ hybridization shows Dmp1 (AG1) to be a developmentally regulated dentin-specific protein produced by mature odontoblasts. Connective Tissue Research. 33, 67-72. George, A., Srinivasan, R., Thotakura, S., Veis, A., 1998. The phosphophoryn gene family: identical domain structures at the carboxyl end. European Journal of Oral Sciences. 106, 221-226. Gu, K., Chang, S., Slaven, M., Clarkson, B., Rutherford, R., Ritchie, H., 1998. Human dentin phosphophoryn nucleotide and amino acid sequence. European Journal of Oral Sciences. 106, 1043-1047. Hay, M. F., 1961. The development in vivo and in vitro of the lower incisor and molars of the mouse. Arch Oral Biol. 3, 86-109. Hirst, K., Ibaraki-O'Connor, K., Young, M., Dixon, M., 1997a. Cloning and expression analysis of the bovine dentin matrix acidic phosphoprotein gene. Journal of dental research. 76, 754-760. Hirst, K., Simmons, D., Feng, J., Aplin, H., Dixon, M., Macdougall, M., 1997b. Elucidation of the sequence and the genomic organization of the human dentin matrix acidic phosphoprotein 1 (DMP1) gene: exclusion of the locus from a causative role in the pathogenesis of dentinogenesis imperfecta type II. Genomics. 42, 38-45. Huang, B., Maciejewska, I., Sun, Y., Peng, T., Qin, D., Lu, Y., Bonewald, L., Butler, W. T., Feng, J., Qin, C., 2008. Identification of full-length dentin matrix protein 1 in dentin and bone. Calcif Tissue Int. 82, 401-410. Huq, N., Loganathan, A., Cross, K., Chen, Y., Johnson, N., Willetts, M., Veith, P., Reynolds, E., 2005. Association of bovine dentine phosphophoryn with collagen fragments. Archives of oral biology. 50, 807-819. Huysseune, A., Van der Heyden, C., Sire, J., 1998. Early development of the zebrafish (Danio rerio) pharyngeal dentition (Teleostei, Cyprinidae). Anatomy and embryology. 198, 289-305. Jacquier, A., Dujon, B., 1985. An intron-encoded protein is active in a gene conversion process that spreads an intron into a mitochondrial gene. Cell. 41, 383-94. Jain, A., Karadag, A., Fohr, B., Fisher, L., Fedarko, N., 2002. Three SIBLINGs(Small Integrin-Binding LIgand, N-linked Glycoproteins) Enhance Factor H's Cofactor Activity Enabling MCP-like Cellular Evasion of Complement-mediated Attack. Journal of Biological Chemistry. 277, 13700-13708. Jernvall, J., Aberg, T., Kettunen, P., Keranen, S., Thesleff, I., 1998. The life history of an embryonic signaling center: BMP-4 induces p21 and is associated with apoptosis in the mouse tooth enamel knot. Development. 125, 161-169. Jernvall, J., Kettunen, P., Karavanova, I., Martin, L., Thesleff, I., 1994. Evidence for the role of the enamel knot as a control center in mammalian tooth cusp formation: non-dividing cells express growth stimulating Fgf-4 gene. International Journal of Developmental Biology. 38, 463-463. Jernvall, J., Thesleff, I., 2000. Reiterative signaling and patterning during mammalian tooth morphogenesis. Mechanisms of development. 92, 19-29. Kaufman, E., Solomon, V., Rozen, L., Peltz, R., 1994. Pulpal anesthesia efficacy of four lidocaine solutions injected with an intraligamentary syringe. Oral surgery, oral medicine, oral pathology. 78, 17-21. Kawakami, K., 2007. Tol2: a versatile gene transfer vector in vertebrates. Genome Biol. 8 Suppl 1, S7. Kawakami, K., Imanaka, K., Itoh, M., Taira, M., 2004a. Excision of the Tol2 transposable element of the medaka fish Oryzias latipes in Xenopus laevis and Xenopus tropicalis. Gene. 338, 93-98. Kawakami, K., Noda, T., 2004. Transposition of the Tol2 element, an Ac-like element from the Japanese medaka fish Oryzias latipes, in mouse embryonic stem cells. Genetics. 166, 895-899. Kawakami, K., Shima, A., Kawakami, N., 2000. Identification of a functional transposase of the Tol2 element, an Ac-like element from the Japanese medaka fish, and its transposition in the zebrafish germ lineage. PNAS. 97, 11403-11408. Kawakami, K., Takeda, H., Kawakami, N., Kobayashi, M., Matsuda, N., Mishina, M., 2004b. A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish. Developmental Cell. 7, 133-144. Kimmel, C., Miller, C., Moens, C., 2001. Specification and morphogenesis of the zebrafish larval head skeleton. Developmental Biology. 233, 239-257. Koga, A., Suzuki, M., Inagaki, H., Bessho, Y., Hori, H., 1996. Transposable element in fish. Nature. 383, 30. Kulkarni, G., Chen, B., Malone, J., Narayanan, A., George, A., 2000. Promotion of selective cell attachment by the RGD sequence in dentine matrix protein 1. Archives of oral biology. 45, 475-484. Linde, A., Goldberg, M., 1993. Dentinogenesis. Critical Reviews in Oral Biology & Medicine. 4, 679-728. Lu, Y., Qin, C., Xie, Y., Bonewald, L., Feng, J., 2009. Studies of the DMP1 57-kDa Functional Domain both in vivo and in vitro. Cells Tissues Organs. 189, 175-185. Lu, Y., Xie, Y., Zhang, S., Dusevich, V., Bonewald, L., Feng, J., 2007. DMP1-targeted Cre expression in odontoblasts and osteocytes. Journal of dental research. 86, 320-325. Lu, Y., Zhang, S., Xie, Y., Pi, Y., Feng, J., 2005. Differential regulation of dentin matrix protein 1 expression during odontogenesis. Cells Tissues Organs. 181, 241-247. MacDougall, M., DuPont, B. R., Simmons, D., Leach, R. J., 1996. Assignment of DMP1 to human chromosome 4 band q21 by in situ hybridization. Cytogenet Cell Genet. 74, 189. MacDougall, M., Gu, T., Luan, X., Simmons, D., Chen, J., 1998. Identification of a novel isoform of mouse dentin matrix protein 1: spatial expression in mineralized tissues. The Journal of Bone and Mineral Research. 13, 422-431. MacDougall, M., Simmons, D., Luan, X., Nydegger, J., Feng, J., Gu, T., 1997. Dentin phosphoprotein and dentin sialoprotein are cleavage products expressed from a single transcript coded by a gene on human chromosome 4. Dentin phosphoprotein DNA sequence determination. Journal of Biological Chemistry. 272, 835-842. Maciejewska, I., Qin, D., Huang, B., Sun, Y., Mues, G., Svoboda, K., Bonewald, L., Butler, W., Feng, J., Qin, C., 2009. Distinct Compartmentalization of Dentin Matrix Protein 1 Fragments in Mineralized Tissues and Cells. Cells Tissues Organs. 189, 186-191. Narayanan, K., Gajjeraman, S., Ramachandran, A., Hao, J., George, A., 2006. Dentin matrix protein 1 regulates dentin sialophosphoprotein gene transcription during early odontoblast differentiation. Journal of Biological Chemistry. 281, 19064-19071. Ogbureke, K., Fisher, L., 2004. Expression of SIBLINGs and Their Partner MMPs in Salivary Glands. Journal of Dental Research. 83, 664-670. Qin, C., Brunn, J., Cook, R., Orkiszewski, R., Malone, J., Veis, A., Butler, W., 2003. Evidence for the Proteolytic Processing of Dentin Matrix Protein 1 IDENTIFICATION AND CHARACTERIZATION OF PROCESSED FRAGMENTS AND CLEAVAGE SITES*. Journal of Biological Chemistry. 278, 34700-34708. Qin, C., Brunn, J., Jones, J., George, A., Ramachandran, A., Gorski, J., Butler, W., 2001. A comparative study of sialic acid-rich proteins in rat bone and dentin. European Journal of Oral Sciences. 109, 133-141. Qin, C., D'Souza, R., Feng, J., 2007. Dentin matrix protein 1 (DMP1): new and important roles for biomineralization and phosphate homeostasis. Journal of dental research. 86, 1134-1141. Ritchie, H., Ritchie, D., Wang, L., 1998. Six decades of dentinogenesis research. Historical and prospective views on phosphophoryn and dentin sialoprotein. European Journal of Oral Sciences. 106, 211-220. Ritchie, H., Wang, L., 1996. Sequence determination of an extremely acidic rat dentin phosphoprotein. Journal of Biological Chemistry. 271, 21695-21698. Ritchie, H., Wang, L., 1997. A mammalian bicistronic transcript encoding two dentin-specific proteins. Biochemical and Biophysical Research Communications. 231, 425-428. Schilling, T., Piotrowski, T., Grandel, H., Brand, M., Heisenberg, C., Jiang, Y., Beuchle, D., Hammerschmidt, M., Kane, D., Mullins, M., 1996. Jaw and branchial arch mutants in zebrafish I: branchial arches. Development. 123, 329-344. Shimizu-Sasaki, E., Yamazaki, M., Furuyama, S., Sugiya, H., Sodek, J., Ogata, Y., 2003. Identification of FGF2-response element in the rat bone sialoprotein gene promoter. Connective Tissue Research. 44, 103-108. Sollner, C., Burghammer, M., Busch-Nentwich, E., Berger, J., Schwarz, H., Riekel, C., Nicolson, T., 2003. Control of crystal size and lattice formation by starmaker in otolith biomineralization. Science. 302, 282-286. Soukup, V., Epperlein, H. H., Horacek, I., Cerny, R., 2008. Dual epithelial origin of vertebrate oral teeth. Nature. 455, 795-8. Terasawa, M., Shimokawa, R., Terashima, T., Ohya, K., Takagi, Y., Shimokawa, H., 2004. Expression of dentin matrix protein 1 (DMP1) in nonmineralized tissues. Journal of Bone and Mineral Metabolism. 22, 430-438. Thotakura, S., Karthikeyan, N., Smith, T., Liu, K., George, A., 2000. Cloning and characterization of rat dentin matrix protein 1 (DMP1) gene and its 5'-upstream region. Journal of Biological Chemistry. 275, 10272-10277. Toyosawa, S., O'hUigin, C., Klein, J., 1999a. The dentin matrix protein 1 gene of prototherian and metatherian mammals. Journal of molecular evolution. 48, 160-167. Toyosawa, S., O'huigin, C., Tichy, H., Klein, J., 1999b. Characterization of dentin matrix protein 1 gene in crocodilia. Gene. 234, 307-314. Toyosawa, S., Okabayashi, K., Komori, T., Ijuhin, N., 2004. mRNA expression and protein localization of dentin matrix protein 1 during dental root formation. Bone. 34, 124-133. Toyosawa, S., Shintani, S., Fujiwara, T., Ooshima, T., Sato, A., Ijuhin, N., Komori, T., 2001. Dentin matrix protein 1 is predominantly expressed in chicken and rat osteocytes but not in osteoblasts. Journal of Bone and Mineral Research. 16, 2017-2026. Tucker, A., Sharpe, P., 2004. The cutting-edge of mammalian development; how the embryo makes teeth. Nature Reviews Genetics. 5, 499-508. Tummers, M., Thesleff, I., 2009. The importance of signal pathway modulation in all aspects of tooth development. J Exp Zoolog B Mol Dev Evol. 312B, 309-319. Vaahtokari, A., Aberg, T., Jernvall, J., Keranen, S., Thesleff, I., 1996a. The enamel knot as a signaling center in the developing mouse tooth. Mechanisms of development. 54, 39-43. Vaahtokari, A., Aberg, T., Thesleff, I., 1996b. Apoptosis in the developing tooth: association with an embryonic signaling center and suppression by EGF and FGF-4. Development. 122, 121-9. Van der Heyden, C., Huysseune, A., Sire, J., 2000. Development and fine structure of pharyngeal replacement teeth in juvenile zebrafish (Danio rerio)(Teleostei, Cyprinidae). Cell and Tissue Research. 302, 205-219. Waltregny, D., Bellahcene, A., Van Riet, I., Fisher, L., Young, M., Fernandez, P., Dewe, W., de Leval, J., Castronovo, V., 1998. Prognostic value of bone sialoprotein expression in clinically localized human prostate cancer. Journal of the National Cancer Institute. 90, 1000-1008. Wang, D., Yamamoto, S., Hijiya, N., Benveniste, E., Gladson, C., 2000. Transcriptional regulation of the human osteopontin promoter: functional analysis and DNA-protein interactions. Oncogene. 19, 5801-5809. Wilson, M., Liaw, L., Koopman, P., 2005. Osteopontin and related SIBLING glycoprotein genes are expressed by Sertoli cells during mouse testis development. Developmental dynamics. 233, 1488-1495. Ye, L., MacDougall, M., Zhang, S., Xie, Y., Zhang, J., Li, Z., Lu, Y., Mishina, Y., Feng, J., 2004. Deletion of dentin matrix protein-1 leads to a partial failure of maturation of predentin into dentin, hypomineralization, and expanded cavities of pulp and root canal during postnatal tooth development. Journal of Biological Chemistry. 279, 19141-19148. Ye, L., Mishina, Y., Chen, D., Huang, H., Dallas, S. L., Dallas, M. R., Sivakumar, P., Kunieda, T., Tsutsui, T. W., Boskey, A., Bonewald, L. F., Feng, J. Q., 2005. Dmp1-deficient mice display severe defects in cartilage formation responsible for a chondrodysplasia-like phenotype. J Biol Chem. 280, 6197-203. Zeichner-David, M., Diekwisch, T., Fincham, A., Lau, E., MacDougall, M., Moradian-Oldak, J., Simmer, J., Snead, M., Slavkin, H., 1995. Odontogenesis: Embryonic dentition as a tool for developmental biology. International Journal of Developmental Biology. 39, 69-92. 余俐亭, 人類DMP-1基因啟動子之選殖及分析. 國立臺灣大學醫學院口腔生物科學研究所口腔細胞生物學組碩士論文, 2004. 林麗芬, 藉由報導基因EGFP探討DMP1在發育過程中的組織專一性表現及構築DMP1啟動子啟動Shh過量表現之結構體. 國立臺灣大學醫學院口腔生物科學研究所碩士論文, 2007. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44140 | - |
dc.description.abstract | 骨骼及牙齒為脊椎動物中兩個主要的礦化組織,而骨生成及齒質形成過程中,骨細胞與牙本質母細胞會分泌非膠原性蛋白,並在細胞外形成基質(ECM),其中一類非膠原性的蛋白稱為SIBLING家族(SIBLING family;Small Integrin-Binding Ligand, N-linked Glycoprotein)。而DMP-1是這家族蛋白的一個成員。這些SIBLING家族的蛋白質具有許多共同的特性,例如,含有大量的唾液酸(sialic acid)、磷酸鹽成分,以及精氨酸-甘氨酸-天門冬氨酸(Arginine-Glycine-Aspartic acid;RGD sequence)細胞結合序列,此類酸性蛋白質在骨頭及牙齒組織的形成及礦化時會被分泌到細胞外基質(ECM)與羥基磷灰石(hydroxyapatite)結合,幫助羥基磷灰石晶體形成與組織礦化。
先前以綠色螢光蛋白EGFP為報導基因,在斑馬魚及小鼠的動物模式中分析人類DMP-1啟動子的活性可發現人類DMP-1啟動子的上游2.783 kb片段對基因的調控在斑馬魚和小鼠間具有保留性(余俐亭,2004),另外也發現DMP-1的表現並非侷限在礦化的組織,在非礦化的組織中也有DMP-1的表現。然而,並未發現綠色螢光有在牙齒表現,因此DMP-1啟動子的上游2.783 kb片段可能並未包括促使DMP-1基因在牙齒表現的序列(林麗芬,2007)。 有鑑於此,我以斑馬魚及小鼠為模式動物試圖找出人類DMP-1基因上下游序列中調控DMP-1在牙齒表現的加強子區域。首先我先在基因庫(UCSC, University of California Santa Cruz, Genome Browser website)中搜尋各物種的DMP-1基因以及其上下游的基因體DNA片段。透過不同物種間DNA序列之相似性比對,在上下游共發現有七段高度保守DNA片段。 接著我以PCR的方法從人類子宮頸癌細胞基因組DNA中分別複製出此七段保守DNA片段,作為候選加強子(enhancer),並以βB1-Crystallin 1.3 kb (Cr1.3)基因在眼睛水晶體表現之加強子(enhancer)片段(-1290 bp ~ -62 bp)作為動物過渡性轉殖實驗中的篩選標記(selection marker),然後將Cr1.3及此七段保守序列分別接合2.783 kb之DMP-1啟動子上游,並且以綠色螢光蛋白(EGFP, enhanced green fluorescent protein)為報導基因。再將這些結構體中含E2(enhancer 2)以顯微注射的方式注入斑馬魚及小鼠之受精卵中,結果在過渡性轉殖實驗中,發現這些結構體能驅使綠色螢光蛋白表現在斑馬魚胚胎的水晶體及咽齒區域;另一方面,在小鼠頭部的切片觀察結果也發現,綠色螢光蛋白在小鼠的水晶體及牙本質母細胞表現。由以上結果推測所選殖的DMP-1上游約18.8 kb的659 bp之E2片段可能包含了調控該基因表現在牙齒之順式調控因子。 | zh_TW |
dc.description.abstract | Bones and teeth are two major mineralized tissues in vertebrate. In the process of osteogenesis and dentinogenesis, osteoblasts and odontoblasts secrete non-collagenous proteins to form extracellular matrix (ECM). One group of the non-collagenous proteins is SIBLING family (SIBLING family; Small-Integrin-Binding Ligand, N-linked Glyco- protein). DMP-1 (Dentin Matrix Protein 1; DMP-1) is a member of the family. The members of SIBLING family share many common characteristics. For example, they have sialic acids, phosphates, and RGD sequence. Such acidic phosphorylated proteins will secrete to ECM to incorporate with hydroxyapatite, helping nucleation of hydroxyapatite crystal growth and mineralization.
Preliminarily, upstream 2,783 kb fragment of human DMP-1 promoter is functional by the expression of the reporter gene (EGFP, enhanced green fluorescent protein) in transgenic zabrafish and mice (Yu, 2004). The results suggest that location of the DMP-1 is not restricted to mineralized tissues and may be present in non-mineralized tissue (Lin, 2007). However, the reporter gene EGFP is not expressed in odontoblasts of teeth. According to this finding, I attempt to indentify tooth-specific enhancer elements of human Dentin Matrix Protein 1 (DMP1) gene. By analyzing sequences which are located in upstream and downstream of DMP-1 gene between different species from UCSC bio-informatics, I found out seven highly conserved DNA sequences. I cloned the DNA fragments of these regions from HeLa cell genome, as candidate enhancers, using βB1-Crystallin 1.3 kb (Cr1.3) enhancer elements (-1290 bp ~ -62 bp), which confer tissue-specificity in lens, as a selection marker in transient transgenic experiments. Then Cr1.3 and the seven DNA fragments of the highly conserved regions were ligated with 2.783 kb human DMP-1 promoter with EGFP as a reporter gene. They were analyzed in zebrafish and mouse by transgenic assay. The constructed plasmid including E2 (Enhancer 2) was injected into both zebrafish and mouse fertilized eggs. The results demonstrated that the reporter is expressed in lens and regions of pharyngeal teeth in zebrafish embryo with E2 enhancer-construct. In addition, the reporter is expressed in lens and odontoblasts in mouse by the same construct. Taken together, the evolutionarily conserved DNA sequence E2 might provide a cis-element for rendering tooth-specificity to human DMP-1 gene. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T02:41:44Z (GMT). No. of bitstreams: 1 ntu-98-R96450010-1.pdf: 6780531 bytes, checksum: cbba8bed8f9025c1258b510fd4eb3b52 (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 口試委員審定書…………………………………………………………i
誌謝………………………………………………………………………ii 中文摘要………………………………………………………………iii 英文摘要…………………………………………………………………v 壹、前言………………………………………………………………1 貳、實驗材料…………………………………………………………21 參、實驗方法…………………………………………………………29 肆、結果………………………………………………………………42 伍、討論………………………………………………………………45 陸、圖表…………………………………………………………………50 參考文獻…………………………………………………………………76 | |
dc.language.iso | zh-TW | |
dc.title | 以基因轉殖方法藉由報導基因 EGFP 辨識人類 DMP1 基因在牙齒專一表現之加強子 | zh_TW |
dc.title | Identification of Tooth-Specific Enhancer Element of Human Dentin Matrix Protein 1 (DMP1) Gene by Transgenic Assays | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 姚宗珍,陳志成 | |
dc.subject.keyword | 牙本質基質蛋白,牙本質母細胞,加強子,斑馬魚,咽齒,綠色螢光蛋白, | zh_TW |
dc.subject.keyword | DMP-1,odontoblast,enhancer,zebrafish,pharyngeal teeth,EGFP, | en |
dc.relation.page | 86 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2009-08-11 | |
dc.contributor.author-college | 牙醫專業學院 | zh_TW |
dc.contributor.author-dept | 口腔生物科學研究所 | zh_TW |
顯示於系所單位: | 口腔生物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-98-1.pdf 目前未授權公開取用 | 6.62 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。