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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張智芬(Zee-Fen, Chang) | |
| dc.contributor.author | Jheng-Guang Jhong | en |
| dc.contributor.author | 鍾政光 | zh_TW |
| dc.date.accessioned | 2021-06-15T02:36:00Z | - |
| dc.date.available | 2009-09-15 | |
| dc.date.copyright | 2009-09-15 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-08-13 | |
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'Contribution of guanine exchange factor H1 in phorbol ester-induced apoptosis.' Cell Death Differ 13(12): 2023-32. Coleman, M. L., E. A. Sahai, et al. (2001). 'Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I.' Nat Cell Biol 3(4): 339-45. Dvorsky, R., L. Blumenstein, et al. (2004). 'Structural insights into the interaction of ROCKI with the switch regions of RhoA.' J Biol Chem 279(8): 7098-104. Etienne-Manneville, S. and A. Hall (2002). 'Rho GTPases in cell biology.' Nature 420(6916): 629-35. Feng, J., M. Ito, et al. (1999). 'Inhibitory phosphorylation site for Rho-associated kinase on smooth muscle myosin phosphatase.' J Biol Chem 274(52): 37385-90. Feng, J., M. Ito, et al. (1999). 'Rho-associated kinase of chicken gizzard smooth muscle.' J Biol Chem 274(6): 3744-52. Fincham, V. J. and M. C. Frame (1998). 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'FAK-Src signalling through paxillin, ERK and MLCK regulates adhesion disassembly.' Nat Cell Biol 6(2): 154-61. Wong, K., O. Pertz, et al. (2006). 'Neutrophil polarization: spatiotemporal dynamics of RhoA activity support a self-organizing mechanism.' Proc Natl Acad Sci U S A 103(10): 3639-44. Yamana, N., Y. Arakawa, et al. (2006). 'The Rho-mDia1 pathway regulates cell polarity and focal adhesion turnover in migrating cells through mobilizing Apc and c-Src.' Mol Cell Biol 26(18): 6844-58. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44006 | - |
| dc.description.abstract | RhoA-regulated cell contractility plays important roles in variety of cellular
processes. Rho-associated coiled–coil kinase (ROCK) is one of the downstream effectors of RhoA signaling and generates cell contraction force by promoting myosin light chain (MLC) phosphorylation. Two isoforms of ROCK, ROCKI and ROCKII, have been characterized. Previously, our laboratory has demonstrated that RhoA-dependent ROCKII activation is negatively regulated by tyrosine phosphorylation of ROCKII, which is controlled by c-Src and Shp2. In this study, I investigated whether ROCKI activation is also regulated by tyrosine phosphorylation. First, I detected tyrosine phosphorylation of ROCKI in HEK293T cells after treatment with pervanadate, a phosphatase inhibitor, and this phosphorylation event requires Src kinase. By expressing of dominant active SrcY527F and performing in vitro assay with c-Src kinase, I demonstrated that c-Src phosphorylates ROCKI in vivo and in vitro. Furthermore, I generated several deletion and YF substitution mutants of flag-ROCKI, and found that Tyr913 residue is one of Src-mediated phosphorylation sites of ROCKI. However, expression of flag-ROCKI(Y913F) had little effect on nocodazole-stimulated RhoA-mediated MLC phosphorylation in NIH3T3 cells, indicating that phosphorylation of Tyr913 may not participate in negative regulation of ROCKI. In summary, I may conclude that in addition to Tyr913, ROCKI has another tyrosine phosphorylation sites, and the tyrosine phosphorylation on regulating ROCKI activity should be investigated further. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T02:36:00Z (GMT). No. of bitstreams: 1 ntu-98-R96442018-1.pdf: 1312319 bytes, checksum: 7be50d0db634cc4ff8f5d5991183b4bf (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 口試委員會審定書......I
誌謝......II 中文摘要......IV 英文摘要......V 前言......1 實驗材料與實驗方法 ......6 實驗結果 ......16 討論......20 圖表 ......24 參考文獻 ......32 附錄 ......35 | |
| dc.language.iso | zh-TW | |
| dc.subject | c-Src | zh_TW |
| dc.subject | RhoA | zh_TW |
| dc.subject | ROCKI | zh_TW |
| dc.title | Tyrosine磷酸化修飾對ROCKI活性調控之探討 | zh_TW |
| dc.title | Regulation of ROCKI by tyrosine phosphorylation | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 許金玉(Jin-Yuh, Shew),李明學(Ming-Shyue, Lee) | |
| dc.subject.keyword | RhoA,ROCKI,c-Src, | zh_TW |
| dc.relation.page | 37 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2009-08-13 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 生物化學暨分子生物學研究所 | zh_TW |
| 顯示於系所單位： | 生物化學暨分子生物學科研究所 | |
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