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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李昆達 | |
| dc.contributor.author | Ching-Yeuh Su | en |
| dc.contributor.author | 蘇敬岳 | zh_TW |
| dc.date.accessioned | 2021-06-15T02:29:29Z | - |
| dc.date.available | 2012-12-17 | |
| dc.date.copyright | 2009-08-20 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-08-17 | |
| dc.identifier.citation | Andersen, D.C., and Krummen, L. (2002). Recombinant protein expression for therapeutic applications. Current opinion in biotechnology 13, 117-123.
Anthony, M.S., Clarkson, T.B., Hughes, C.L., Jr., Morgan, T.M., and Burke, G.L. (1996). Soybean isoflavones improve cardiovascular risk factors without affecting the reproductive system of peripubertal rhesus monkeys. Jurnal of Nutrition 126, 43-50. Binns, A.N. (2002). T-DNA of Agrobacterium tumefaciens: 25 years and counting. Trends in plant science 7, 231-233. Birch, R.G. (1997). Plant Transformation: problems and strategies for practical application. Annual review of plant physiology and plant molecular biology 48, 297-326. Boevink, P., Martin, B., Oparka, K., Santa Cruz, S., and Hawes, C. (1999). Transport of virally expressed green fluorescent protein through the secretory pathway in tobacco leaves is inhibited by cold shock and brefeldin A. Planta 208, 392-400. Bross, P., Andresen, B.S., Winter, V., Krautle, F., Jensen, T.G., Nandy, A., Kolvraa, S., Ghisla, S., Bolund, L., and Gregersen, N. (1993). Co-overexpression of bacterial GroESL chaperonins partly overcomes non-productive folding and tetramer assembly of E. coli-expressed human medium-chain acyl-CoA dehydrogenase (MCAD) carrying the prevalent disease-causing K304E mutation. Biochimica et biophysica acta 1182, 264-274. Capell, T., and Christou, P. (2004). Progress in plant metabolic engineering. Current opinion in biotechnology 15, 148-154. Cappelletti, V., Fioravanti, L., Miodini, P., and Di Fronzo, G. (2000). Genistein blocks breast cancer cells in the G(2)M phase of the cell cycle. Journal of Cellular Biochemistry 79, 594-600. Chang, C.K., Chang, K.S., Lin, Y.C., Liu, S.Y., and Chen, C.Y. (2005). Hairy root cultures of Gynostemma pentaphyllum (Thunb.) Makino: a promising approach for the production of gypenosides as an alternative of ginseng saponins. Biotechnology letters 27, 1165-1169. Chiang, P.Y. (2006). Expression of green fluorescent protein in tobacco hairy roots. In Institute of Microbiology and Biochemistry (Taipei: National Taiwan University). Christey, M.C. (1997). Transgenic crop plants using Agrobacterium rhizogenes-mediated transformation. In Hairy Roots—Culture and Applications, P.M. Doran, ed (Harwood Academic, Australia), pp. 99-111. Cotter, A., and Cashman, K.D. (2003). Genistein appears to prevent early postmenopausal bone loss as effectively as hormone replacement therapy. Nutrition Reviews 61, 346-351. Cubitt, A.B., Heim, R., Adams, S.R., Boyd, A.E., Gross, L.A., and Tsien, R.Y. (1995). Understanding, improving and using green fluorescent proteins. Trends in biochemical sciences 20, 448-455. Dicosmo, F., and Misawa, M. (1995). Plant cell and tissue culture: alternatives for metabolite production. Biotechnology advances 13, 425-453. Doran, P.M. (2000). Foreign protein production in plant tissue cultures. Current opinion in biotechnology 11, 199-204. Finnegan, J., and McElroy, D. (1994). Transgene Inactivation: Plants Fight Back! Nature biotechnology 12, 883 - 888 Gelvin, S.B. (1998). The introduction and expression of transgenes in plants. Current opinion in biotechnology 9, 227-232. Goodman-Gruen, D., and Kritz-Silverstein, D. (2001). Usual dietary isoflavone intake is associated with cardiovascular disease risk factors in postmenopausal women. Journal of Nutritions 131, 1202-1206. Guillon, S., Tremouillaux-Guiller, J., Pati, P.K., Rideau, M., and Gantet, P. (2006). Hairy root research: recent scenario and exciting prospects. Current Opinion in Plant Biology 9, 341-346. Heim, R., and Tsien, R.Y. (1996). Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Current Biology 6, 178-182. Hellens, R.P., Allan, A.C., Friel, E.N., Bolitho, K., Grafton, K., Templeton, M.D., Karunairetnam, S., Gleave, A.P., and Laing, W.A. (2005). Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1, 13. Hellwig, S., Drossard, J., Twyman, R.M., and Fischer, R. (2004). Plant cell cultures for the production of recombinant proteins. Nature biotechnology 22, 1415-1422. Hezari, M., and Croteau, R. (1997). Taxol biosynthesis: an update. Planta Medica 63, 291-295. Hezari, M., Ketchum, R.E., Gibson, D.M., and Croteau, R. (1997). Taxol production and taxadiene synthase activity in Taxus canadensis cell suspension cultures. Archives of Biochemistry and Biophysics 337, 185-190. James, E., and Lee, J.M. (2001). The production of foreign proteins from genetically modified plant cells. Advances in Biochemical Engineering / Biotechnology 72, 127-156. Jefferson, R.A., Kavanagh, T.A., and Bevan, M.W. (1987). GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. The EMBO journal 6, 3901-3907. Kereszt, A., Li, D., Indrasumunar, A., Nguyen, C.D., Nontachaiyapoom, S., Kinkema, M., and Gresshoff, P.M. (2007). Agrobacterium rhizogenes-mediated transformation of soybean to study root biology. Nature Protocols 2, 948-952. Kuo, C.C. (2008). Agrobacterium rhizogenes-mediated soybean gene transformation. In Institute of Microbiology and Biochemistry (Taipei: National Taiwan University). Larrick, J.W., Yu, L., Naftzger, C., Jaiswal, S., and Wycoff, K. (2001). Production of secretory IgA antibodies in plants. Biomolecular Engineering 18, 87-94. Li, C., Schwabe, J.W., Banayo, E., and Evans, R.M. (1997). Coexpression of nuclear receptor partners increases their solubility and biological activities. Proc Natl Acad Sci U S A 94, 2278-2283. Liu, H.W. (2004). Expression of a heat inducible promoter of Arabidopsis in tobacco hairy roots. In Institute of Microbiology and Biochemistry (Taipei: National Taiwan University). Lozovaya, V.V., Lygin, A.V., Zernova, O.V., Ulanov, A.V., Li, S., Hartman, G.L., and Widholm, J.M. (2007). Modification of phenolic metabolism in soybean hairy roots through down regulation of chalcone synthase or isoflavone synthase. Planta 225, 665-679. Miura, T., Yuan, L., Sun, B., Fujii, H., Yoshida, M., Wakame, K., and Kosuna, K. (2002). Isoflavone aglycon produced by culture of soybean extracts with basidiomycetes and its anti-angiogenic activity. Bioscience Biotechnology and Biochemistry 66, 2626-2631. Murashige, T., and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15, 473-497. Novick, R.P. (1987). Plasmid incompatibility. Microbiological Reviews 51, 381-395. Novick, R.P., Clowes, R.C., Cohen, S.N., Curtiss, R., 3rd, Datta, N., and Falkow, S. (1976). Uniform nomenclature for bacterial plasmids: a proposal. Bacteriological Reviews 40, 168-189. Ohta, S., Mita, S., Hattori, T., and Nakamura, K. (1990). Construction and expression in tobacco of a b-Glucuronidase (GUS) reporter gene containing an intron within the coding sequence Plant and Cell Physiology 31, 805-813. Palazon, J., Mallol, A., Eibl, R., Lettenbauer, C., Cusido, R.M., and Pinol, M.T. (2003). Growth and ginsenoside production in hairy root cultures of Panax ginseng using a novel bioreactor. Planta Medica 69, 344-349. Ravindranath, M.H., Muthugounder, S., Presser, N., and Viswanathan, S. (2004). Anticancer therapeutic potential of soy isoflavone, genistein. Advances in Experimental Medicine and Biology 546, 121-165. Rivas, F.V., Tolia, N.H., Song, J.J., Aragon, J.P., Liu, J., Hannon, G.J., and Joshua-Tor, L. (2005). Purified Argonaute2 and an siRNA form recombinant human RISC. Nat Struct Mol Biol 12, 340-349. Sevón, N., and Oksman-Caldentey, K.M. (2002). Agrobacterium rhizogenes-mediated transformation: root cultures as a source of alkaloids. Planta Medica 68, 859-868. Sijmons, P.C., Dekker, B.M., Schrammeijer, B., Verwoerd, T.C., van den Elzen, P.J., and Hoekema, A. (1990). Production of correctly processed human serum albumin in transgenic plants. Biotechnology (N Y) 8, 217-221. Taketomi, E.A., Silva, D.A., Sopelete, M.C., Gervasio, A.M., Alves, R., and Sung, S.J. (2006). Differential IgE reactivity to Der p 1 and Der p 2 allergens of Dermatophagoides pteronyssinus in mite-sensitized patients. J Investig Allergol Clin Immunol 16, 104-109. Twyman, R.M., Schillberg, S., and Fischer, R. (2005). Transgenic plants in the biopharmaceutical market. Expert Opinion on Emerging Drugs 10, 185-218. Tzfira, T., and Citovsky, V. (2006). Agrobacterium-mediated genetic transformation of plants: biology and biotechnology. Current opinion in biotechnology 17, 147-154. Weaver, C.M., and Cheong, J.M. (2005). Soy isoflavones and bone health: the relationship is still unclear. Journal of Nutrition 135, 1243-1247. Wojciechowski, M.F., Mahn, J., and Jones, B. (2006). Fabaceae. In The Tree of Life Web Project (http://www.tolweb.org/tree/). Xiang, T.H., Wang, L.L., Pang, J.L., Chen, M., and Xu, C. (2005). Hairy root induced by wild-type Agrobacterium rhizogenes K599 in soybean, cucumber and garden balsam in vivo. Herediats (Beijing) 27, 783-786. Yamamura, Y., Sahin, F.P., Nagatsu, A., and Mizukami, H. (2003). Molecular cloning and characterization of a cDNA encoding a novel apoplastic protein preferentially expressed in a shikonin-producing callus strain of Lithospermum erythrorhizon. Plant and Cell Physiology 44, 437-446. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43816 | - |
| dc.description.abstract | 本論文在進行根毛農桿菌對菸草之多重基因共轉形之探討。菸草威斯康新 38 號被選為農桿菌感染研究用材料。本研究選用GFP與GUS兩個報導蛋白質之基因進行菸草之多基因共轉形。本研究在設計上有兩個策略，策略一為一個雙元質體攜帶一個報導基因。亦即：單一菌體同時攜帶雙元質體 (2BV)，或兩質體分別位於兩農桿菌來同時感染菸草 (2AR)。策略二為，一個雙元質體內含有雙報導基因：我們建構了兩個質體，分別是兩個報導基因位於同一個 T-DNA 區域中 (pCGG-1TD)，或兩個基因位於二個不同的 T-DNA 區域中 (pCGG-2TD)。四類欲用來探討菸草多基因轉形策略的農桿菌已建構完成，並已分別對菸草進行感染。2AR 及 2BV 二組的誘導率分別為 81％ 及75％，而帶有雙基因的雙元質體組 (pCGG-1TD 與pCGG-2TD) 誘導率很低。報導基因表現方面，2BV 及 pCGG-1TD 兩組已經以螢光顯微鏡及 b-葡萄糖酸苷酶染色技術證實該兩組可同時表現綠色螢光蛋白及 b-葡萄糖酸苷酶。2BV 組是目前較好的共轉形及共表現策略。在本篇研究中，如果各個策略皆可順利將基因轉形至植物中，我們將可同時運用兩種以上的策略來對植物進行多基因的轉形，以得到可大量生產植物次級代謝物的毛狀根。 | zh_TW |
| dc.description.abstract | Transformation of multiple genes into tobacco by Agrobacterium rhizogenes-mediated method was performed in this study. Nicotiana tabacum Wisconsin 38 was selected as the material for hairy root induction. Two reporter genes, gfp from pCAMBIA 1302 and gus from pCAMBIA 1201, were used for co-transformation study. Two strategies were developed. The first one, two reporter genes were carried by two respective plasmids. And then, transformation was conducted either by one transformed A. rhizogenes with two binary vectors (2BV), or conducted by co-cultured at the same time with two separate transformed A. rhizogenes which harboring one reporter gene (2AR). The second one was, constructing the two reporter genes in one binary vector. Thus, two reporter genes located in one T-DNA region or separated in two T-DNA regions were constructed, and they were named pCGG-1TD and pCGG-2TD, respectively. The above plasmids were transformed into A rhizogenes, and then inoculated tobacco leaf discs. The root inducing rate of 2AR and 2BV were 81 % and 75 %, respectively. On the other hand, those induced by pCGG-1TD and pCGG-2TD caused less hairy roots. Regarding expression of reporter genes, in 2BV and pCGG-1TD groups, we have observed GFP expression by fluorescence microscopy and GUS expression by tissue staining. Nowadays, the construction of 2BV was the better strategy to gain co-transformation and co-expression hairy roots. In this study, if all strategies can succeed to transform multiple genes into plants, we can integrate those strategies and transform multi-genes into plants at the same time to induce hairy roots for secondary metabolite production. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T02:29:29Z (GMT). No. of bitstreams: 1 ntu-98-R95b47309-1.pdf: 3421703 bytes, checksum: 24ee8c8477a99d9f782219ccefe7aa8c (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 謝誌.....................................................II
Abstract................................................III 中文摘要..................................................V Abbreviations............................................VI Index...................................................VII Contents...............................................VIII Contents of tables and figures...........................XI Chapter 1 Introduction....................................1 1.1 Transgenic plant cell cultures......................2 1.1.1 Plant cell cultures.............................2 1.1.2 Agrobacterium-mediated gene transformation......4 1.1.3 Hairy root cultures.............................7 1.2 Multiple genes co-expression........................8 1.3 Reporter proteins...................................9 1.4 Research aim .......................................11 Chapter 2 Materials and Methods..........................12 2.1 Plasmid constructions..............................13 2.1.1 Binary vectors.................................13 2.1.2 Two reporter genes in one T-DNA region.........14 2.1.3 Two T-DNA regions in one binary vector.........16 2.2 Bacteria transformation methods....................18 2.2.1 Heat shock method for E. coli transformation...18 2.2.2 Electroporation for A. rhizogenes transformation ................................................18 2.2.3 PCR confirmation...............................20 2.3 Establishment of hairy root clones.................21 2.3.1 tobacco sterile plants germination.............21 2.3.2 Preparation of A. rhizogenes for induction of tobacco hairy roots............................21 2.3.3 Induction of tobacco hairy roots...............22 2.3.4 Sterilization of hairy roots...................22 2.3.5 Liquid cultures of hairy roots.................23 2.4 Confirmation of foreign DNA in root clones.........23 2.4.1 Extraction of genomic DNA......................23 2.4.2 Confirmation of foreign genes with PCR.........24 2.5 Functional GFP and GUS confirmation................25 2.5.1 Direct observation of GFP......................25 2.5.2 GUS activity assay.............................26 2.6 Protein quantification.............................27 2.6.1 Extraction of cytosol protein..................27 2.6.2 Quantification of total protein................27 2.6.3 ELISA assay for detection of GFP...............27 Chapter 3 Results and Discussions........................29 3.1 Construction of plasmid pCGG-1TD and pCGG-2TD......30 3.2 Establishment of 2AR and 2BV transgenic root clones ...................................................32 3.3 Induction rate of hairy roots......................34 Chapter 4 Perspectives...................................35 Tables and Figures.......................................38 References...............................................59 Appendix.................................................67 A.1 Molecular cloning..................................68 A.2 Microbe culture....................................69 A.3 Plant culture......................................69 A.4 Protein purification and detection.................70 | |
| dc.language.iso | en | |
| dc.subject | 根毛農桿菌 | zh_TW |
| dc.subject | 菸草 | zh_TW |
| dc.subject | b-葡萄糖酸苷 | zh_TW |
| dc.subject | 綠色螢光蛋白質 | zh_TW |
| dc.subject | 毛狀根 | zh_TW |
| dc.subject | GUS | en |
| dc.subject | GFP | en |
| dc.subject | hairy roots | en |
| dc.subject | tobacco | en |
| dc.subject | Agrobacterium rhizogenes | en |
| dc.title | 根毛農桿菌對菸草之多基因轉形研究 | zh_TW |
| dc.title | Agrobacterium rhizogenes-mediated genes transformation of Nicotiana tabacum Wisconsin 38 | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蘇遠志,楊健志,劉裕國,劉啟德 | |
| dc.subject.keyword | 根毛農桿菌,菸草,毛狀根,綠色螢光蛋白質,b-葡萄糖酸苷,酶, | zh_TW |
| dc.subject.keyword | Agrobacterium rhizogenes,tobacco,hairy roots,GFP,GUS, | en |
| dc.relation.page | 71 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2009-08-17 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
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