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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 吳益群(Yi-Chun Wu) | |
| dc.contributor.author | Hui Chiu | en |
| dc.contributor.author | 邱卉 | zh_TW |
| dc.date.accessioned | 2021-06-15T02:27:24Z | - |
| dc.date.available | 2013-08-20 | |
| dc.date.copyright | 2011-08-20 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-17 | |
| dc.identifier.citation | 1. Neumann B, Nguyen KC, Hall DH, Ben-Yakar A, Hilliard MA. Axonal regeneration proceeds through specific axonal fusion in transected C. elegans neurons. Dev Dyn 2011; 240:1365-72.
2. Yanik MF, Cinar H, Cinar HN, Chisholm AD, Jin Y, Ben-Yakar A. Neurosurgery: functional regeneration after laser axotomy. Nature 2004; 432:822. 3. Cajal Ry. Degeneration and Regeneration of the Nervous System. Oxford Univ Press, 1928. 4. Chernoff EA, Sato K, Corn A, Karcavich RE. Spinal cord regeneration: intrinsic properties and emerging mechanisms. Semin. Cell Dev Biol 2002; 13:361-8. 5. Case LC, Tessier-Lavigne M. Regeneration of the adult central nervous system. Curr Biol 2005; 15:749-53. 6. Cai D, Qiu J, Cao Z, McAtee M, Bregman BS, Filbin MT. Neuronal cyclic AMP controls the developmental loss in ability of axons to regenerate. J Neurosci 2001; 21:4731-9. 7. Goldberg JL, Klassen MP, Hua Y, Barres BA. Amacrine-signaled loss of intrinsic axon growth ability by retinal ganglion cells. Science 2002; 296:1860-4. 8. Qiu J, Cai D, Dai H, McAtee M, Hoffman PN, Bregman BS, Filbin MT. Spinal axon regeneration induced by elevation of cyclic AMP. Neuron 2002; 34:895-903. 9. Park KK, Liu K, Hu Y, Smith PD, Wang C, Cai B, Xu B, Connolly L, Kramvis I, Sahin M, He Z. Promoting axon regeneration in the adult CNS by modulation of the PTEN/mTOR pathway. Science 2008; 322:963-6. 10. Moore DL, Blackmore MG, Hu Y, Kaestner KH, Bixby JL, Lemmon VP, Goldberg JL. KLF family members regulate intrinsic axon regeneration ability. Science 2009; 326:298-301. 11. Bareyre FM, Garzorz N, Lang C, Misgeld T, Buning H, Kerschensteiner M. In vivo imaging reveals a phase-specific role of STAT3 during central and peripheral nervous system axon regeneration. Proc Natl Acad Sci U S A 2011; 108:6282-7. 12. David S, Aguayo AJ. Axonal elongation into peripheral nervous system 'bridges' after central nervous system injury in adult rats. Science 1981; 214:931-3. 13. Filbin MT. Recapitulate development to promote axonal regeneration: good or bad approach? Philos Trans R Soc Lond B Biol Sci 2006; 361:1565-74. 14. Rudge JS, Silver J. Inhibition of neurite outgrowth on astroglial scars in vitro. J Neurosci 1990; 10:3594-603. 15. Silver J, Miller JH. Regeneration beyond the glial scar. Nat. Rev. Neurosci. 2004; 5:146-56. 16. Fitch MT, Silver J. Glial cell extracellular matrix: boundaries for axon growth in development and regeneration. Cell Tissue Res 1997; 290:379-84. 17. Fawcett JW, Asher RA. The glial scar and central nervous system repair. Brain Res Bull 1999; 49:377-91. 18. Morgenstern DA, Asher RA, Fawcett JW. Chondroitin sulphate proteoglycans in the CNS injury response. Prog Brain Res 2002; 137:313-32. 19. Berry M. Post-injury myelin-breakdown products inhibit axonal growth: an hypothesis to explain the failure of axonal regeneration in the mammalian central nervous system. Bibl Anat 1982:1-11. 20. McKerracher L, David S, Jackson DL, Kottis V, Dunn RJ, Braun PE. Identification of myelin-associated glycoprotein as a major myelin-derived inhibitor of neurite growth. Neuron 1994; 13:805-11. 21. Mukhopadhyay G, Doherty P, Walsh FS, Crocker PR, Filbin MT. A novel role for myelin-associated glycoprotein as an inhibitor of axonal regeneration. Neuron 1994; 13:757-67. 22. Chen MS, Huber AB, van der Haar ME, Frank M, Schnell L, Spillmann AA, Christ F, Schwab ME. Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1. Nature 2000; 403:434-9. 23. GrandPre T, Nakamura F, Vartanian T, Strittmatter SM. Identification of the Nogo inhibitor of axon regeneration as a Reticulon protein. Nature 2000; 403:439-44. 24. Prinjha R, Moore SE, Vinson M, Blake S, Morrow R, Christie G, Michalovich D, Simmons DL, Walsh FS. Inhibitor of neurite outgrowth in humans. Nature 2000; 403:383-4. 25. Wang KC, Koprivica V, Kim JA, Sivasankaran R, Guo Y, Neve RL, He Z. Oligodendrocyte-myelin glycoprotein is a Nogo receptor ligand that inhibits neurite outgrowth. Nature 2002; 417:941-4. 26. Fournier AE, GrandPre T, Strittmatter SM. Identification of a receptor mediating Nogo-66 inhibition of axonal regeneration. Nature 2001; 409:341-6. 27. Domeniconi M, Cao Z, Spencer T, Sivasankaran R, Wang K, Nikulina E, Kimura N, Cai H, Deng K, Gao Y, He Z, Filbin M. Myelin-associated glycoprotein interacts with the Nogo66 receptor to inhibit neurite outgrowth. Neuron 2002; 35:283-90. 28. Liu BP, Fournier A, GrandPre T, Strittmatter SM. Myelin-associated glycoprotein as a functional ligand for the Nogo-66 receptor. Science 2002; 297:1190-3. 29. Woolf CJ. No Nogo: now where to go? Neuron 2003; 38:153-6. 30. Zheng B, Atwal J, Ho C, Case L, He XL, Garcia KC, Steward O, Tessier-Lavigne M. Genetic deletion of the Nogo receptor does not reduce neurite inhibition in vitro or promote corticospinal tract regeneration in vivo. Proc Natl Acad Sci U S A 2005; 102:1205-10. 31. De Winter F, Oudega M, Lankhorst AJ, Hamers FP, Blits B, Ruitenberg MJ, Pasterkamp RJ, Gispen WH, Verhaagen J. Injury-induced class 3 semaphorin expression in the rat spinal cord. Exp Neurol 2002; 175:61-75. 32. Manitt C, Colicos MA, Thompson KM, Rousselle E, Peterson AC, Kennedy TE. Widespread expression of netrin-1 by neurons and oligodendrocytes in the adult mammalian spinal cord. J Neurosci 2001; 21:3911-22. 33. Low K, Culbertson M, Bradke F, Tessier-Lavigne M, Tuszynski MH. Netrin-1 is a novel myelin-associated inhibitor to axon growth. J Neurosci 2008; 28:1099-108. 34. Moreau-Fauvarque C, Kumanogoh A, Camand E, Jaillard C, Barbin G, Boquet I, Love C, Jones EY, Kikutani H, Lubetzki C, Dusart I, Chedotal A. The transmembrane semaphorin Sema4D/CD100, an inhibitor of axonal growth, is expressed on oligodendrocytes and upregulated after CNS lesion. J Neurosci 2003; 23:9229-39. 35. Goldberg JL, Vargas ME, Wang JT, Mandemakers W, Oster SF, Sretavan DW, Barres BA. An oligodendrocyte lineage-specific semaphorin, Sema5A, inhibits axon growth by retinal ganglion cells. J Neurosci 2004; 24:4989-99. 36. Kantor DB, Chivatakarn O, Peer KL, Oster SF, Inatani M, Hansen MJ, Flanagan JG, Yamaguchi Y, Sretavan DW, Giger RJ, Kolodkin AL. Semaphorin 5A is a bifunctional axon guidance cue regulated by heparan and chondroitin sulfate proteoglycans. Neuron 2004; 44:961-75. 37. Benson MD, Romero MI, Lush ME, Lu QR, Henkemeyer M, Parada LF. Ephrin-B3 is a myelin-based inhibitor of neurite outgrowth. Proc Natl Acad Sci U S A 2005; 102:10694-9. 38. Pasterkamp RJ, De Winter F, Giger RJ, Verhaagen J. Role for semaphorin III and its receptor neuropilin-1 in neuronal regeneration and scar formation? Prog Brain Res 1998; 117:151-70. 39. Pasterkamp RJ, Giger RJ, Ruitenberg MJ, Holtmaat AJ, De Wit J, De Winter F, Verhaagen J. Expression of the gene encoding the chemorepellent semaphorin III is induced in the fibroblast component of neural scar tissue formed following injuries of adult but not neonatal CNS. Mol Cell Neurosci 1999; 13:143-66. 40. Miranda JD, White LA, Marcillo AE, Willson CA, Jagid J, Whittemore SR. Induction of Eph B3 after spinal cord injury. Exp Neurol 1999; 156:218-22. 41. Wu Z, Ghosh-Roy A, Yanik MF, Zhang JZ, Jin Y, Chisholm AD. Caenorhabditis elegans neuronal regeneration is influenced by life stage, ephrin signaling, and synaptic branching. Proc Natl Acad Sci U S A 2007; 104:15132-7. 42. Gabel CV, Antoine F, Chuang CF, Samuel AD, Chang C. Distinct cellular and molecular mechanisms mediate initial axon development and adult-stage axon regeneration in C. elegans. Development 2008; 135:1129-36. 43. Ferretti P, Zhang F, O'Neill P. Changes in spinal cord regenerative ability through phylogenesis and development: lessons to be learnt. Dev Dyn 2003; 226:245-56. 44. Hammarlund M, Nix P, Hauth L, Jorgensen EM, Bastiani M. Axon regeneration requires a conserved MAP kinase pathway. Science 2009; 323:802-6. 45. Dotti CG, Banker GA. Experimentally induced alteration in the polarity of developing neurons. Nature 1987; 330:254-6. 46. Goslin K, Banker G. Experimental observations on the development of polarity by hippocampal neurons in culture. J Cell Biol 1989; 108:1507-16. 47. Hayashi K, Kawai-Hirai R, Ishikawa K, Takata K. Reversal of neuronal polarity characterized by conversion of dendrites into axons in neonatal rat cortical neurons in vitro. Neuroscience 2002; 110:7-17. 48. Bradke F, Dotti CG. Differentiated neurons retain the capacity to generate axons from dendrites. Curr Biol 2000; 10:1467-70. 49. Neumann S, Woolf CJ. Regeneration of dorsal column fibers into and beyond the lesion site following adult spinal cord injury. Neuron 1999; 23:83-91. 50. Ghosh-Roy A, Wu Z, Goncharov A, Jin Y, Chisholm AD. Calcium and cyclic AMP promote axonal regeneration in Caenorhabditis elegans and require DLK-1 kinase. J Neurosci 2010; 30:3175-83. 51. Hoy RR, Bittner GD, Kennedy D. Regeneration in crustacean motoneurons: evidence for axonal fusion. Science 1967; 156:251-2. 52. Birse SC, Bittner GD. Regeneration of giant axons in earthworms. Brain Res 1976; 113:575-81. 53. Deriemer SA, Elliott EJ, Macagno ER, Muller KJ. Morphological evidence that regenerating axons can fuse with severed axon segments. Brain Res 1983; 272:157-61. 54. Macagno ER, Muller KJ, DeRiemer SA. Regeneration of axons and synaptic connections by touch sensory neurons in the leech central nervous system. J Neurosci 1985; 5:2510-21. 55. Cai D, Shen Y, De Bellard M, Tang S, Filbin MT. Prior exposure to neurotrophins blocks inhibition of axonal regeneration by MAG and myelin via a cAMP-dependent mechanism. Neuron 1999; 22:89-101. 56. Neumann S, Bradke F, Tessier-Lavigne M, Basbaum AI. Regeneration of sensory axons within the injured spinal cord induced by intraganglionic cAMP elevation. Neuron 2002; 34:885-93. 57. Nikulina E, Tidwell JL, Dai HN, Bregman BS, Filbin MT. The phosphodiesterase inhibitor rolipram delivered after a spinal cord lesion promotes axonal regeneration and functional recovery. Proc Natl Acad Sci U S A 2004; 101:8786-90. 58. Nakata K, Abrams B, Grill B, Goncharov A, Huang X, Chisholm AD, Jin Y. Regulation of a DLK-1 and p38 MAP kinase pathway by the ubiquitin ligase RPM-1 is required for presynaptic development. Cell 2005; 120:407-20. 59. Hammarlund M, Davis WS, Jorgensen EM. Mutations in beta-spectrin disrupt axon outgrowth and sarcomere structure. J Cell Biol 2000; 149:931-42. 60. Lewcock JW, Genoud N, Lettieri K, Pfaff SL. The ubiquitin ligase Phr1 regulates axon outgrowth through modulation of microtubule dynamics. Neuron 2007; 56:604-20. 61. Campbell DS, Holt CE. Apoptotic pathway and MAPKs differentially regulate chemotropic responses of retinal growth cones. Neuron 2003; 37:939-52. 62. Verma P, Chierzi S, Codd AM, Campbell DS, Meyer RL, Holt CE, Fawcett JW. Axonal protein synthesis and degradation are necessary for efficient growth cone regeneration. J Neurosci 2005; 25:331-42. 63. Brisbin S, Liu J, Boudreau J, Peng J, Evangelista M, Chin-Sang I. A role for C. elegans Eph RTK signaling in PTEN regulation. Dev Cell 2009; 17:459-69. 64. Kaczynski J, Cook T, Urrutia R. Sp1- and Kruppel-like transcription factors. Genome Biol 2003; 4:206. 65. Sulston JE, White JG. Regulation and cell autonomy during postembryonic development of Caenorhabditis elegans. Dev Biol 1980; 78:577-97. 66. Oraevsky AA, Silva LBD, Rubenchik AM, Feit MD, Glinsky ME, Perry MD, Mammini BM, Small IV M, Stuart BC. Plasma mediated ablation of biological tissues with nanosecond-to-femtosecond laser pulses: relative role of linear and nonlinear absorption. IEEE J Sel Topics in Quantum Electron 1996; 2:801-9. 67. Khodjakov A, La Terra S, Chang F. Laser microsurgery in fission yeast; role of the mitotic spindle midzone in anaphase B. Curr Biol 2004; 14:1330-40. 68. Yanik MF, Cinar H, Cinar HN, Gibby A, Chisholm AD, Jin Y, Ben-Yakar A. Nerve Regeneration in Caenorhabditis elegans After Femtosecond Laser Axotomy. IEEE J Sel Topics in Quantum Electron 2006; 12:1283-91. 69. Chung SH, Mazur E. Femtosecond laser ablation of neurons in C. elegans for behavioral studies. Appl Phys A 2009; 96:335-41. 70. Bourgeois F, Ben-Yakar A. Femtosecond laser nanoaxotomy properties and their effect on axonal recovery in C. elegans. Opt Express 2008; 16:5963. 71. Rohde CB, Zeng F, Gonzalez-Rubio R, Angel M, Yanik MF. Microfluidic system for on-chip high-throughput whole-animal sorting and screening at subcellular resolution. Proc Natl Acad Sci U S A 2007; 104:13891-5. 72. Guo SX, Bourgeois F, Chokshi T, Durr NJ, Hilliard MA, Chronis N, Ben-Yakar A. Femtosecond laser nanoaxotomy lab-on-a-chip for in vivo nerve regeneration studies. Nat Methods 2008; 5:531-3. 73. Zeng F, Rohde CB, Yanik MF. Sub-cellular precision on-chip small-animal immobilization, multi-photon imaging and femtosecond-laser manipulation. Lab Chip 2008; 8:653-6. 74. Ben-Yakar A, Chronis N, Lu H. Microfluidics for the analysis of behavior, nerve regeneration, and neural cell biology in C. elegans. Curr Opin Neurobiol 2009; 19:561-7. 75. Wahl SM, Arend WP, Ross R. The effect of complement depletion on wound healing. Am J Pathol 1974; 75:73-89. 76. Vargas ME, Watanabe J, Singh SJ, Robinson WH, Barres BA. Endogenous antibodies promote rapid myelin clearance and effective axon regeneration after nerve injury. Proc Natl Acad Sci U S A 2010; 107:11993-8. 77. Farah MH, Pan BH, Hoffman PN, Ferraris D, Tsukamoto T, Nguyen T, et al. Reduced BACE1 activity enhances clearance of myelin debris and regeneration of axons in the injured peripheral nervous system. J Neurosci 2011; 31:5744-54. 78. Filbin MT. Myelin-associated inhibitors of axonal regeneration in the adult mammalian CNS. Nat Rev Neurosci 2003; 4:703-13. 79. Rabinovitch M. Professional and non-professional phagocytes: an introduction. Trends Cell Biol 1995; 5:85-7. 80. Parnaik R, Raff MC, Scholes J. Differences between the clearance of apoptotic cells by professional and non-professional phagocytes. Curr Biol 2000; 10:857-60. 81. Zhou Z, Hartwieg E, Horvitz HR. CED-1 is a transmembrane receptor that mediates cell corpse engulfment in C. elegans. Cell 2001; 104:43-56. 82. Franc NC, Dimarcq JL, Lagueux M, Hoffmann J, Ezekowitz RA. Croquemort, a novel Drosophila hemocyte/macrophage receptor that recognizes apoptotic cells. Immunity 1996; 4:431-43. 83. Platt N, Suzuki H, Kurihara Y, Kodama T, Gordon S. Role for the class A macrophage scavenger receptor in the phagocytosis of apoptotic thymocytes in vitro. Proc Natl Acad Sci U S A 1996; 93:12456-60. 84. Franc NC, Heitzler P, Ezekowitz RA, White K. Requirement for croquemort in phagocytosis of apoptotic cells in Drosophila. Science 1999; 284:1991-4. 85. Manaka J, Kuraishi T, Shiratsuchi A, Nakai Y, Higashida H, Henson P, et al. Draper-mediated and phosphatidylserine-independent phagocytosis of apoptotic cells by Drosophila hemocytes/macrophages. J Biol Chem 2004; 279:48466-76. 86. Gardai SJ, McPhillips KA, Frasch SC, Janssen WJ, Starefeldt A, Murphy-Ullrich JE, et al. Cell-surface calreticulin initiates clearance of viable or apoptotic cells through trans-activation of LRP on the phagocyte. Cell 2005; 123:321-34. 87. Hamon Y, Trompier D, Ma Z, Venegas V, Pophillat M, Mignotte V, et al. Cooperation between engulfment receptors: the case of ABCA1 and MEGF10. PLoS One 2006; 1:e120. 88. Kurant E, Axelrod S, Leaman D, Gaul U. Six-microns-under acts upstream of Draper in the glial phagocytosis of apoptotic neurons. Cell 2008; 133:498-509. 89. Wu HH, Bellmunt E, Scheib JL, Venegas V, Burkert C, Reichardt LF, et al. Glial precursors clear sensory neuron corpses during development via Jedi-1, an engulfment receptor. Nat Neurosci 2009; 12:1534-41. 90. Awasaki T, Tatsumi R, Takahashi K, Arai K, Nakanishi Y, Ueda R, et al. Essential role of the apoptotic cell engulfment genes draper and ced-6 in programmed axon pruning during Drosophila metamorphosis. Neuron 2006; 50:855-67. 91. MacDonald JM, Beach MG, Porpiglia E, Sheehan AE, Watts RJ, Freeman MR. The Drosophila cell corpse engulfment receptor Draper mediates glial clearance of severed axons. Neuron 2006; 50:869-81. 92. Ellis RE, Jacobson DM, Horvitz HR. Genes required for the engulfment of cell corpses during programmed cell death in Caenorhabditis elegans. Genetics 1991; 129:79-94. 93. Wu YC, Horvitz HR. The C. elegans cell corpse engulfment gene ced-7 encodes a protein similar to ABC transporters. Cell 1998; 93:951-60. 94. Su HP, Nakada-Tsukui K, Tosello-Trampont AC, Li Y, Bu G, Henson PM, et al. Interaction of CED-6/GULP, an adapter protein involved in engulfment of apoptotic cells with CED-1 and CD91/low density lipoprotein receptor-related protein (LRP). J Biol Chem 2002; 277:11772-9. 95. Liu QA, Hengartner MO. Candidate adaptor protein CED-6 promotes the engulfment of apoptotic cells in C. elegans. Cell 1998; 93:961-72. 96. Yu X, Odera S, Chuang CH, Lu N, Zhou Z. Dynamin mediates the signaling of phagocytic receptor CED-1 for the engulfment and degradation of apoptotic cells. Dev Cell 2006; 10:743-57. 97. Hamon Y, Broccardo C, Chambenoit O, Luciani MF, Toti F, Chaslin S, et al. ABC1 promotes engulfment of apoptotic cells and transbilayer redistribution of phosphatidylserine. Nat Cell Biol 2000; 2:399-406. 98. Wu YC, Horvitz HR. C. elegans phagocytosis and cell-migration protein CED-5 is similar to human DOCK180. Nature 1998; 392:501-4. 99. Reddien PW, Horvitz HR. CED-2/CrkII and CED-10/Rac control phagocytosis and cell migration in Caenorhabditis elegans. Nat Cell Biol 2000; 2:131-6. 100. Gumienny TL, Brugnera E, Tosello-Trampont AC, Kinchen JM, Haney LB, Nishiwaki K, et al. CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration. Cell 2001; 107:27-41. 101. Wu YC, Tsai MC, Cheng LC, Chou CJ, Weng NY. C. elegans CED-12 acts in the conserved crkII/DOCK180/Rac pathway to control cell migration and cell corpse engulfment. Dev Cell 2001; 1:491-502. 102. Zhou Z, Caron E, Hartwieg E, Hall A, Horvitz HR. The C. elegans PH domain protein CED-12 regulates cytoskeletal reorganization via a Rho/Rac GTPase signaling pathway. Dev Cell 2001; 1:477-89. 103. Brugnera E, Haney L, Grimsley C, Lu M, Walk SF, Tosello-Trampont AC, et al. Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex. Nat Cell Biol 2002; 4:574-82. 104. Wang X, Wu YC, Fadok VA, Lee MC, Gengyo-Ando K, Cheng LC, et al. Cell corpse engulfment mediated by C. elegans phosphatidylserine receptor through CED-5 and CED-12. Science 2003; 302:1563-6. 105. Lu M, Kinchen JM, Rossman KL, Grimsley C, Hall M, Sondek J, et al. A Steric-inhibition model for regulation of nucleotide exchange via the Dock180 family of GEFs. Curr Biol 2005; 15:371-7. 106. Cabello J, Neukomm LJ, Gunesdogan U, Burkart K, Charette SJ, Lochnit G, et al. The Wnt pathway controls cell death engulfment, spindle orientation, and migration through CED-10/Rac. PLoS Biol 2010; 8:e1000297. 107. Hsu TY, Wu YC. Engulfment of apoptotic cells in C. elegans is mediated by integrin alpha/SRC signaling. Curr Biol 2010; 20:477-86. 108. Kinchen JM, Cabello J, Klingele D, Wong K, Feichtinger R, Schnabel H, et al. Two pathways converge at CED-10 to mediate actin rearrangement and corpse removal in C. elegans. Nature 2005; 434:93-9. 109. Waller A. Experiments on the section of the glossopharyngeal and hypoglossal nerves of the frog, and observations of the alterations produced thereby in the structure of their primitive fibres. Philos T R Soc Lon B 1850; 140:423-9. 110. Lunn ER, Perry VH, Brown MC, Rosen H, Gordon S. Absence of Wallerian degeneration does not hinder regeneration in peripheral nerve. Eur J Neurosci 1989; 1:27-33. 111. Bignami A, Dahl D, Nguyen BT, Crosby CJ. The fate of axonal debris in Wallerian degeneration of rat optic and sciatic nerves. Electron microscopy and immunofluorescence studies with neurofilament antisera. J Neuropathol Exp Neurol 1981; 40:537-50. 112. George R, Griffin JW. Delayed macrophage responses and myelin clearance during Wallerian degeneration in the central nervous system: the dorsal radiculotomy model. Exp Neurol 1994; 129:225-36. 113. Brenner S. The genetics of Caenorhabditis elegans. Genetics 1974; 77:71-94. 114. Mello C, Fire A. DNA transformation. Methods Cell Biol 1995; 48:451-82. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43742 | - |
| dc.description.abstract | 在模式物種線蟲 Caenorhabditis elegans 中，周邊神經受傷害之後引起兩件重要的生物性事件同時發生：軸突碎屑的產生以及神經再生的促進。過去的研究並未解釋這兩件事如何彼此調控。在這篇研究中，我們觀察發現，軸突被截斷之後的幾分鐘之內，其中尚與神經細胞本體連結的軸突會於末梢崩落出局部性的軸突碎屑。這樣只侷限分佈於受傷位置的局部性軸突碎屑並未被描述於過去的神經再生研究中，並且，它與經由瓦勒式退化所產生之軸突碎屑十分不同。瓦勒式退化所產生的軸突碎屑指的是軸突被截斷之後, 其中與神經細胞分離的那一整節軸突會於受傷的幾天之內瓦解成不連續的片段。有趣的是，我們發現局部性軸突碎屑的清除和神經再生的啟動之間有緊密的關聯性。我們推測這可能說明了局部性軸突碎屑的清除是啟動神經再生的必要前置作業，或者，吞噬細胞在清除局部性軸突碎屑的過程中也能夠同時促進神經再生。過去在不同物種的研究中已經指出吞噬細胞清除軸突碎屑的過程是可能受到吞噬基因的調控。我們的研究結果發現吞噬基因也參與了神經再生的調控。若吞噬基因之一的鳥嘌呤轉換因子 CED-5/DOCK180 發生突變會抑制線蟲的神經再生能力。於 ced-5 突變蟲體中，當正常功能的 CED-5 藉由細胞專一表現啟動子特定表現在神經細胞內部或包含了體壁肌肉在內的三種吞噬細胞中可以恢復神經再生的能力。本篇研究結果顯示了線蟲的體壁細胞是如何藉由鳥嘌呤轉換因子 ced-5 的作用促進神經再生。 | zh_TW |
| dc.description.abstract | Two important biological events happen coincidently after axon injury in the PNS neurons of Caenorhabditis elegans. One is the generation of axon debris and the other is the promotion of axon regeneration. It is not known how these two events are regulated and related to each other. In this report, we observed a local shedding of the axon debris from the proximal end of the severed axon within minutes after axon injury. This type of axon debris is previously undescribed and distinctive from the axon debris generated by Wallerian degeneration, which is the distal segment of the severed axon becomes globally fragmented within days after axon injury. Interestingly, the removal of the proximal-end axon debris appears to coincide with the initiation of axon regeneration. We reasoned that either the removal of the proximal-end axon debris is a prerequisite for initiating axon regeneration or the engulfment cells required for removing axon debris is also utilized for promoting axon regeneration. Our results suggested that CED-5/DOCK180 guanine nucleotide exchange factor plays an important role in regulating axon regeneration since mutations in ced-5 caused significantly reduced axon regeneration. Furthermore, cell specific rescue experiments showed that expression of ced-5 in either the touch neurons or another three types of somatic cells fully rescued the defective axon regeneration phenotypes in ced-5 mutants. Thus, our study revealed the cell autonomous and non-cell autonomous roles of ced-5 in axon regeneration. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T02:27:24Z (GMT). No. of bitstreams: 1 ntu-100-R97b43008-1.pdf: 918198 bytes, checksum: 635efcd458463aa574fb3d27bd18db06 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 論文口試委員審定書
中文摘要 i Abstract ii Table of Contents iii I. Introduction 1 1.1 C. elegans as a genetic model to identify novel cellular and molecular mechanisms underlying nervous system regeneration 1 1.2 The canonical pathways involved in the engulfment of apoptotic cells 13 II. Materials and methods 16 2.1 Strains 16 2.2 The creation of transgenic animals 16 2.3 Microscopy 16 2.4 Femtosecond laser surgery 17 2.5 Measuring regenerative axon length 17 2.6 Plasmid construction 18 III. Results 20 3.1 The initiation of axon regeneration is associated with the disappearance of axon debris 20 3.2 The differential roles of engulfment genes in axon regeneration 21 3.3 ced-5 functions both cell autonomously in the touch neurons and non-cell autonomously in multiple types of cells to affect ALM axon regeneration 21 IV. Discussion 23 4.1 The discovery of the extrinsic factors that promote axon regeneration 23 V. Reference 24 VI. Figures 31 Figure 1. Analogous counterparts of the human nervous systems in C. elegans 31 Figure 2. Study of axon regeneration using femtosecond laser axotomy and live imaging 33 Figure 3. The axon debris accumulation is tightly linked with the axon regeneration initiation 35 Figure 4. Selective engulfment genes are essential for axon regeneration 37 Figure 5. The expression pattern of a 1.3-Kb ced-5 promoter::GFP reporter in the adult animals 44 Figure 6. ced-5 has both cell autonomous and non-cell autonomous roles in regulating ALM axon regeneration 41 VII. Tables 43 7.1 The conserved patterns and molecular mechanisms of axon regeneration between vertebrates and C. elegans 43 7.2 The list of the transgenes used in this study 44 | |
| dc.language.iso | en | |
| dc.subject | 鳥嘌呤轉換因子 | zh_TW |
| dc.subject | 線蟲 | zh_TW |
| dc.subject | 飛秒雷射軸突截斷技術 | zh_TW |
| dc.subject | 神經再生 | zh_TW |
| dc.subject | 吞噬作用 | zh_TW |
| dc.subject | Femtosecond laser axotomy | en |
| dc.subject | C. elegans | en |
| dc.subject | (Guanine nucleotide Exchange Factor) GEF | en |
| dc.subject | Engulfment | en |
| dc.subject | Axon regeneration | en |
| dc.title | 線蟲吞噬細胞藉由 ced-5/DOCK180 促進神經再生 | zh_TW |
| dc.title | The engulfment cells promote axon regeneration via CED-5/DOCK180 in C. elegans | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 掌杰(Chieh Chang) | |
| dc.contributor.oralexamcommittee | 莊秋芬(Chiou-Fen Chuang),張俊哲(Chun-Che Chang),廖秀娟(Hsiu-Chuan Liao),潘俊良(Chun-Liang Pan) | |
| dc.subject.keyword | 線蟲,飛秒雷射軸突截斷技術,神經再生,吞噬作用,鳥嘌呤轉換因子, | zh_TW |
| dc.subject.keyword | C. elegans,Femtosecond laser axotomy,Axon regeneration,Engulfment,(Guanine nucleotide Exchange Factor) GEF, | en |
| dc.relation.page | 46 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-17 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
| Appears in Collections: | 分子與細胞生物學研究所 | |
Files in This Item:
| File | Size | Format | |
|---|---|---|---|
| ntu-100-1.pdf Restricted Access | 896.68 kB | Adobe PDF |
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