請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43666完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鄧述諄 | |
| dc.contributor.author | Zih-Jie Shen | en |
| dc.contributor.author | 沈子傑 | zh_TW |
| dc.date.accessioned | 2021-06-15T02:25:33Z | - |
| dc.date.available | 2014-09-15 | |
| dc.date.copyright | 2009-09-15 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-08-18 | |
| dc.identifier.citation | Arneric, M., and Lingner, J. (2007). Tel1 kinase and subtelomere-bound Tbf1 mediate preferential elongation of short telomeres by telomerase in yeast. EMBO Rep 8, 1080-1085.
Artandi, S.E., and DePinho, R.A. (2000). A critical role for telomeres in suppressing and facilitating carcinogenesis. Curr Opin Genet Dev 10, 39-46. Bianchi, A., Negrini, S., and Shore, D. (2004). Delivery of yeast telomerase to a DNA break depends on the recruitment functions of Cdc13 and Est1. Mol Cell 16, 139-146. Bianchi, A., and Shore, D. (2007). Increased association of telomerase with short telomeres in yeast. Genes Dev 21, 1726-1730. Bryan, T.M., Englezou, A., Dalla-Pozza, L., Dunham, M.A., and Reddel, R.R. (1997). Evidence for an alternative mechanism for maintaining telomere length in human tumors and tumor-derived cell lines. Nat Med 3, 1271-1274. Chandra, A., Hughes, T.R., Nugent, C.I., and Lundblad, V. (2001). Cdc13 both positively and negatively regulates telomere replication. Genes Dev 15, 404-414. Counter, C.M., Meyerson, M., Eaton, E.N., and Weinberg, R.A. (1997). The catalytic subunit of yeast telomerase. Proc Natl Acad Sci U S A 94, 9202-9207. Cuvier, O., Stanojcic, S., Lemaitre, J.M., and Mechali, M. (2008). A topoisomerase II-dependent mechanism for resetting replicons at the S-M-phase transition. Genes Dev 22, 860-865. de Lange, T. (2002). Protection of mammalian telomeres. Oncogene 21, 532-540. Dunham, M.A., Neumann, A.A., Fasching, C.L., and Reddel, R.R. (2000). Telomere maintenance by recombination in human cells. Nat Genet 26, 447-450. Frank, C.J., Hyde, M., and Greider, C.W. (2006). Regulation of telomere elongation by the cyclin-dependent kinase CDK1. Mol Cell 24, 423-432. Gao, H., Cervantes, R.B., Mandell, E.K., Otero, J.H., and Lundblad, V. (2007). RPA-like proteins mediate yeast telomere function. Nature structural & molecular biology 14, 208-214. Greider, C.W., and Blackburn, E.H. (1985). Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell 43, 405-413. Heinrich, J.N., Kwak, S.P., Howland, D.S., Chen, J., Sturner, S., Sullivan, K., Lipinski, K., Cheng, K.Y., She, Y., Lo, F., et al. (2006). Disruption of ShcA signaling halts cell proliferation--characterization of ShcC residues that influence signaling pathways using yeast. Cell Signal 18, 795-806. Henneke, G., Koundrioukoff, S., and Hubscher, U. (2003). Multiple roles for kinases in DNA replication. EMBO Rep 4, 252-256. Hochstrasser, M. (1996). Ubiquitin-dependent protein degradation. Annu Rev Genet 30, 405-439. Lanker, S., Valdivieso, M.H., and Wittenberg, C. (1996). Rapid degradation of the G1 cyclin Cln2 induced by CDK-dependent phosphorylation. Science 271, 1597-1601. Lendvay, T.S., Morris, D.K., Sah, J., Balasubramanian, B., and Lundblad, V. (1996). Senescence mutants of Saccharomyces cerevisiae with a defect in telomere replication identify three additional EST genes. Genetics 144, 1399-1412. Li, S., Makovets, S., Matsuguchi, T., Blethrow, J.D., Shokat, K.M., and Blackburn, E.H. (2009). Cdk1-dependent phosphorylation of Cdc13 coordinates telomere elongation during cell-cycle progression. Cell 136, 50-61. Lin, J.J., and Zakian, V.A. (1995). An in vitro assay for Saccharomyces telomerase requires EST1. Cell 81, 1127-1135. Lin, J.J., and Zakian, V.A. (1996). The Saccharomyces CDC13 protein is a single-strand TG1-3 telomeric DNA-binding protein in vitro that affects telomere behavior in vivo. Proc Natl Acad Sci U S A 93, 13760-13765. Lingner, J., Hughes, T.R., Shevchenko, A., Mann, M., Lundblad, V., and Cech, T.R. (1997). Reverse transcriptase motifs in the catalytic subunit of telomerase. Science 276, 561-567. Margottin-Goguet, F., Hsu, J.Y., Loktev, A., Hsieh, H.M., Reimann, J.D., and Jackson, P.K. (2003). Prophase destruction of Emi1 by the SCF(betaTrCP/Slimb) ubiquitin ligase activates the anaphase promoting complex to allow progression beyond prometaphase. Dev Cell 4, 813-826. McEachern, M.J., Krauskopf, A., and Blackburn, E.H. (2000). Telomeres and their control. Annu Rev Genet 34, 331-358. Nugent, C.I., Hughes, T.R., Lue, N.F., and Lundblad, V. (1996). Cdc13p: a single-strand telomeric DNA-binding protein with a dual role in yeast telomere maintenance. Science 274, 249-252. Nugent, C.I., and Lundblad, V. (1998). The telomerase reverse transcriptase: components and regulation. Genes Dev 12, 1073-1085. Pennock, E., Buckley, K., and Lundblad, V. (2001). Cdc13 delivers separate complexes to the telomere for end protection and replication. Cell 104, 387-396. Qi, H., and Zakian, V.A. (2000). The Saccharomyces telomere-binding protein Cdc13p interacts with both the catalytic subunit of DNA polymerase alpha and the telomerase-associated est1 protein. Genes Dev 14, 1777-1788. Reddel, R.R., Bryan, T.M., Colgin, L.M., Perrem, K.T., and Yeager, T.R. (2001). Alternative lengthening of telomeres in human cells. Radiat Res 155, 194-200. Sabourin, M., Tuzon, C.T., and Zakian, V.A. (2007). Telomerase and Tel1p preferentially associate with short telomeres in S. cerevisiae. Mol Cell 27, 550-561. Singer, M.S., and Gottschling, D.E. (1994). TLC1: template RNA component of Saccharomyces cerevisiae telomerase. Science 266, 404-409. Steiner, B.R., Hidaka, K., and Futcher, B. (1996). Association of the Est1 protein with telomerase activity in yeast. Proc Natl Acad Sci U S A 93, 2817-2821. Taggart, A.K., Teng, S.C., and Zakian, V.A. (2002). Est1p as a cell cycle-regulated activator of telomere-bound telomerase. Science 297, 1023-1026. Teixeira, M.T., and Gilson, E. (2006). When CDK1 rides the telomere cycle. Mol Cell 24, 491-492. Tseng, S.-F., Lin, J.-J., and Teng, S.-C. (2006a). The telomerase-recruitment domain of the telomere binding protein Cdc13 is regulated by Mec1p/Tel1p-dependent phosphorylation. Nucl Acids Res 34, 6327-6336. Tseng, S.F., Chang, C.Y., Wu, K.J., and Teng, S.C. (2005). Importin KPNA2 Is Required for Proper Nuclear Localization and Multiple Functions of NBS1. J Biol Chem 280, 39594-39600. Tseng, S.F., Lin, J.J., and Teng, S.C. (2006b). The telomerase-recruitment domain of the telomere binding protein Cdc13 is regulated by Mec1p/Tel1p-dependent phosphorylation. Nucleic Acids Res 34, 6327-6336. Tseng, S.F., Shen, Z.J., Tsai, H.J., Lin, Y.H., and Teng, S.C. (2009). Rapid Cdc13 turnover and telomere length homeostasis are controlled by Cdk1-mediated phosphorylation of Cdc13. Nucleic Acids Res 37, 3602-3611. Tsukamoto, Y., Mitsuoka, C., Terasawa, M., Ogawa, H., and Ogawa, T. (2005). Xrs2p regulates Mre11p translocation to the nucleus and plays a role in telomere elongation and meiotic recombination. Mol Biol Cell 16, 597-608. Ubersax, J.A., Woodbury, E.L., Quang, P.N., Paraz, M., Blethrow, J.D., Shah, K., Shokat, K.M., and Morgan, D.O. (2003). Targets of the cyclin-dependent kinase Cdk1. Nature 425, 859-864. Vega, L.R., Mateyak, M.K., and Zakian, V.A. (2003). Getting to the end: telomerase access in yeast and humans. Nat Rev Mol Cell Biol 4, 948-959. Virta-Pearlman, V., Morris, D.K., and Lundblad, V. (1996). Est1 has the properties of a single-stranded telomere end-binding protein. Genes Dev 10, 3094-3104. Vodenicharov, M.D., and Wellinger, R.J. (2006). DNA degradation at unprotected telomeres in yeast is regulated by the CDK1 (Cdc28/Clb) cell-cycle kinase. Mol Cell 24, 127-137. Zakian, V.A. (1996). Structure, function, and replication of Saccharomyces cerevisiae telomeres. Annu Rev Genet 30, 141-172. Zhou, J., Hidaka, K., and Futcher, B. (2000). The Est1 subunit of yeast telomerase binds the Tlc1 telomerase RNA. Mol Cell Biol 20, 1947-1955. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43666 | - |
| dc.description.abstract | 在酵母菌 Saccharomyces cerevisiae中，端粒體蛋白質Cdc13 的功能在於藉由延攬端粒酶延長端立體以及保護染色體末端結構。 在整個細胞分裂的過程中，蛋白質Cdc13 的表現量以及被磷酸化的程度從G1時期往G2/M 時期達到最高峰，而在繼續往G1時期會逐漸減少。在先前的研究指出，蛋白質Rsp5參與裂解單股DNA結合蛋白質Rpa1。而且，Cdc13和Rpa1的結構相類似。因此推測Cdc13的裂解可能經由蛋白質Rsp5所調控。此外，蛋白質Cdc13和Rad53都被DNA損傷反應蛋白激酶Mec1所磷酸化。 而且，Rad53的去磷酸化經由蛋白質Ptc2/Ptc3和Pph3所完成。在本篇研究中，我發現蛋白質Pph3在生體內(in vivo)調控Cdc13的去磷酸化。總結，這兩項發現可推測端粒酶延長端粒體長度的功能可能經由Rsp5 和Pph3對於Cdc13的表現量降低及去磷酸化程度來終止。 | zh_TW |
| dc.description.abstract | In Saccharomyces cerevisiae, the telomere binding protein Cdc13 activates telomere replication by recruiting telomerase, and also performs an essential function in chromosome end protection. Through cell cycle progression, the protein expression level and phosphorylated status of Cdc13 is highest in G2/M phase and decreases in G1 phase. In previous studies, Rsp5 is involved in the degradation of the ssDNA binding protein, Rpa1. Since Cdc13 and Rpa1 share a similar domain organization, I speculate that Cdc13p level is regulated through proteasome-dependent degradation. I found that Rsp5 is a specific ubiquitin E3 ligase of Cdc13. Moreover, Cdc13 and Rad53 are both phosphorylated by the DNA damage-responsive protein kinase, Mec1. Rad53 dephosphorylation has been shown to be dependent on the presence of the PP2C-like phosphatase Ptc2/Ptc3 and PPh3. I found that Pph3 mediates Cdc13 dephosphorylation from G2/M to G1 phase. Take together; these findings suggest that both proteins degradation and dephosphorylation promote telomerase inactivation at the M phase. When cells need to stop telomere replication and enter the next stage of cell cycle. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T02:25:33Z (GMT). No. of bitstreams: 1 ntu-98-R96445124-1.pdf: 765686 bytes, checksum: c456f43be97acb77ededd847eaf3baec (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 口試委員審定書. ii
中文摘要. iii Abstract. iv Contents. v Figure Contents. vi Chapter 1: Introduction of telomere . 1 Chapter 2: Materials and Methods . 5 Chapter 3: Results . 7 3.1 Cdc13p degradation is proteasome-dependent 7 3.2 Cdc13 protein level at steady state is increased in rsp5-1 strain. 7 3.3 Rsp5p interact with Cdc13p in vivo and in vitro 8 3.4 The stability of Cdc13-T308A is increased 9 3.5 Pph3 mediates the dephosphorylation of Cdc13. 10 3.6 Pph3 does not dephosphorylate the phosphorylation by Cdk1. 11 Chapter 4: Discussion. 12 Figures. 14 References. 20 | |
| dc.language.iso | en | |
| dc.subject | 去磷酸化 | zh_TW |
| dc.subject | 端粒體 | zh_TW |
| dc.subject | 蛋白質裂解 | zh_TW |
| dc.subject | telomere | en |
| dc.subject | proteolysis | en |
| dc.subject | dephosphorylation | en |
| dc.title | 蛋白質Rsp5和Pph3 調控磷酸化之蛋白質Cdc13及其訊息傳遞 | zh_TW |
| dc.title | Rsp5 and Pph3 switch off the signals of phosphorylation of Cdc13 | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林敬哲,李財坤 | |
| dc.subject.keyword | 端粒體,蛋白質裂解,去磷酸化, | zh_TW |
| dc.subject.keyword | telomere,proteolysis,dephosphorylation, | en |
| dc.relation.page | 25 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2009-08-18 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-98-1.pdf 未授權公開取用 | 747.74 kB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。