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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林璧鳳(Bi-Fong Lin) | |
| dc.contributor.author | Zhi-Xiong Kang | en |
| dc.contributor.author | 康智雄 | zh_TW |
| dc.date.accessioned | 2021-06-15T02:24:04Z | - |
| dc.date.available | 2014-08-20 | |
| dc.date.copyright | 2009-08-20 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-08-18 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43596 | - |
| dc.description.abstract | 肥胖者體內處於低程度發炎現象而導致了胰島素抗性，脂肪組織內的巨噬細胞型態也影響著胰島素敏感性，有研究指出脂肪細胞所分泌的IL-4會促進M2巨噬細胞生成而具有抗發炎增加胰島素敏感性的功效。本研究首先以3T3-L1脂肪細胞株，篩選具促進IL-4分泌量及降低lipopolysaccharide (LPS) 刺激下 IL-6與MCP-1促發炎細胞激素分泌量之抗胰島素抗性潛力食材樣品。篩選結果顯示，苦瓜(bitter gourd powder, BGP)、靈芝免疫調節蛋白(Ganoderma microsporum immunomodulatory protein, GMI)及食品級靈芝免疫調節蛋白 (f-GMI)均可顯著促進IL-4分泌且抑制成熟脂肪細胞IL-6與MCP-1。
正常飲食 (AIN-76) 情況下，給予C57BL/6J小鼠餵食GMI後具有降低血糖的效果。但在高油飲食誘發肥胖的動物模式下，餵食BGP、GMI與f-GMI，5週後發現f-GMI與BGP都具有降低空腹血糖的功效，而BGP更具有降低HOMA-IR index的能力。 In vivo，體重與脂肪組織IL-4基因表現量達顯著負相關 (r = -0.46, p＝0.0001) ，血糖與脂肪組織IL-4基因表現量兩項指標也有顯著負相關 (r = -0.31, p＝0.01)，顯示體重及血糖增加會降低脂肪組織IL-4的表現量；體重與脾臟分泌IL-4含量也呈顯著負相關 (r = -0.28, p ＝0.01)，顯示肥胖者體內較傾向Th1免疫反應。在脂肪組織，BGP與f-GMI均可顯著促進IL-4表現及M2 巨噬細胞生成，肝臟中M2 巨噬細胞生成的現象也顯著較HF組高。在高油飲食誘發胖小鼠肥胖模式下，餵食BGP與f-GMI的小鼠脾臟細胞在Con A刺激下，IFN-gamma 及IL-2分泌量顯著下降，顯示此兩食材樣品均有助於改善肥胖小鼠體內傾向Th1免疫反應的情形。綜合以上實驗結果得知，BGP及f-GMI可能藉由提高IL-4表現量，促進脂肪組織及肝臟M2 巨噬細胞生成；以及降低Th1細胞激素IFN-gamma及IL-2而具有改善肥胖發炎及胰島素抗性的現象。 | zh_TW |
| dc.description.abstract | Obesity is associated with low-grade system inflammation that results in insulin resistance. Adipose tissue macrophage (ATM) phenotype switch could affect insulin sensitivity. Adipocyte-derived IL-4 could promote M2 macrophage phenotype switch as well as ameliorate insulin resistance. First, using 3T3-L1 cell culture, potential dietary components which could increase IL-4 secretion as well as inhibit LPS-stimulated pro-inflammatory cytokines, IL-6 and MCP-1, were identified. The results showed BGP (Bitter gourd poeder, BGP), GMI(Ganoderma Modulatory Immunoglobulin, GMI) and f-GMI (Food Ganoderma Modulatory Immunoglobulin, f-GMI) could inhibit pro-inflammation cytokines secretion and increase IL-4 secretion. These dietary components were subsequently tested to verify their ability to ameliorate insulin resistance. In vivo, GMI improved blood sugar of C57BL/6J mice that were fed the AIN-76 diet with 50% sucrose. Improvement of fasting blood sugar could be observed in C57BL/6J mice that were fed a 30% fat diet supplemented with BGP or f-GMI for 5 weeks. BGP also decreased HOMA-IR index. BGP increased IL-4 mRNA expression in adipose tissue as well as M2 phenotype switch in adipose tissue and liver. f-GMI increased M2 phenotype switch in adipose tissue. In liver, f-GMI increased M2 phenotype switch and IL-4 mRNA expression. BGP and f-GMI significantly lowered IFN-gamma and IL-2 secretion from Con A-stimulated splenocytes. Furthermore, negative correlations were observed between body weight and IL-4 mRNA expression in adipose tissue (r = －0.46, p ＝＜0.0001), and between blood sugar and IL-4 mRNA expression in adipose tissue (r = －0.31, p ＝0.01). The results show high body weight and blood sugar could inhibit IL-4 mRNA expression in adipose tissue. There was also a negative correlation between body weight and IL-4 secretion from splenocyte (r = －0.28, p ＝0.01), which suggests that obesity is prone to Th1 immune response.
In conclusion, BGP and f-GMI increase M2 phenotype switch in adipose tissue and liver through IL-4 secretion. BGP and f-GMI could reduce IFN-gamma and IL-2 secretion from Con A-stimulated splenocytes. The aforementioned dietary components ameliorate insulin resistance through their inhibition of Th1 immune response abilities. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T02:24:04Z (GMT). No. of bitstreams: 1 ntu-98-R96b47301-1.pdf: 2988875 bytes, checksum: 427c637251dad5728a2c773386b15a2f (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 中文摘要.........................................................i
英文摘要.........................................................ii 第一章、緒言 第一節 文獻回顧..................................................1 一、 代謝症候群之定義..........................................1 二、 胰島素抗性................................................3 三、 肥胖引起的發炎反應........................................5 四、 白色脂肪組織..............................................7 五、 肥胖、代謝症候群與免疫功能缺陷............................10 六、 肥胖與腸道菌相............................................11 七、 靈芝......................................................12 八、 苦瓜......................................................13 九、 Thiazolidinedione........................................... 14 十、 水楊酸....................................................15 第二節 研究動機..................................................16 第三節 研究架構與目的............................................17 一、 研究架構..................................................17 二、 研究目的..................................................17 第二章、食材對脂肪細胞分泌細胞激素之影響 第一節 前言......................................................19 第二節 材料與方法................................................20 一、 細胞株....................................................20 二、 培養基與藥品劑............................................20 三、 實驗樣品..................................................20 四、 儀器設備..................................................21 五、 脂肪細胞實驗..............................................22 六、 細胞激素含量測定..........................................24 七、 統計分析..................................................25 第三節 結果......................................................26 一、 實驗樣品對LPS刺激前脂肪細胞分泌IL-6與MCP-1的影響......26 二、 實驗樣品對LPS刺激成熟脂肪細胞分泌IL-6與MCP-1的影響....26 三、 實驗樣品對成熟脂肪細胞分泌IL-4的影響..................... 26 第四節 討論...................................................... 32 第三章、GMI及f-GMI不同飲食C57BL/6J小鼠血糖及免疫反應的影響 第一節 前言......................................................34 第二節 材料與方法................................................34 一、 實驗設計..................................................34 二、 實驗飼料配製..............................................36 三、 動物飼養..................................................37 四、 樣品收集..................................................39 五、 口服葡萄糖耐受性試驗......................................39 六、 血清葡萄糖分析............................................40 七、 血清胰島素分析............................................40 八、 血清三酸甘油酯分析........................................41 九、 血清膽固醇分析............................................41 十、 胰島素抗性指標 HOMA-IR index 計算........................ 42 十一、血清中總抗體測定.........................................42 十二、脾臟細胞之取得與培養.....................................43 十三、脾臟細胞增生測定.........................................44 十四、腹腔細胞之取得與培養.....................................45 十五、細胞激素含量測定.........................................46 十六、吞噬細胞活性測定.........................................46 十七、肝臟脂質分析.............................................46 十八、Real-time PCR 法分析腹部脂肪組織及肝臟中細胞激素相關基因..48 十九、統計分析................................................. 51 第三節 結果......................................................52 一、 體重變化及攝食量..........................................52 二、 組織器官絕對及相對重量....................................52 三、 血清葡萄糖、三酸甘油酯及膽固醇分析結果....................52 四、 胰島素抗性指標 HOMA-IR index............................. 54 五、 肝臟脂質分析..............................................54 六、 腹部脂肪組織及肝臟細胞激素相關基因表現量..................54 七、 脾臟細胞細胞激素分泌量....................................55 八、 脾臟細胞增生反應..........................................55 九、 血清中總 IgG、IgA與IgM抗體含量......................... 55 十、 腹腔細胞細胞激素分泌量................................... 56 十一、循環血液中吞噬細胞之吞噬能力.............................56 第四節 討論...................................................... 76 第四章、高油飲食補充苦瓜、Rosiglitazone、水楊酸及f-GMI對小鼠的血糖及免疫調節 第一節 前言......................................................80 第二節 材料與方法................................................81 一、 實驗設計..................................................81 二、 實驗飼料配製..............................................82 三、 動物飼養..................................................84 四、 樣品收集..................................................84 五、 口服葡萄糖耐受性試驗......................................84 六、 血清葡萄糖分析............................................84 七、 血清胰島素分析............................................84 八、 血清三酸甘油酯分析........................................84 九、 血清膽固醇分析............................................84 十、 胰島素抗性指標 HOMA-IR index 計算........................ 85 十一、血清中總抗體測定.........................................85 十二、脾臟細胞之取得與培養.....................................85 十三、脾臟細胞增生測定.........................................85 十四、腹腔細胞之取得與培養.....................................85 十五、細胞激素含量測定.........................................85 十六、吞噬細胞活性測定.........................................85 十七、肝臟脂質分析.............................................85 十八、Real-time PCR 法分析腹部脂肪組織及肝臟中細胞激素相關基因..86 十九、腸道初代細胞激素測定.....................................86 二十、統計分析................................................. 86 第三節 結果......................................................87 一、 體重變化及攝食量..........................................87 二、 組織器官絕對及相對重量....................................87 三、 血清葡萄糖、三酸甘油酯及膽固醇分析結果....................88 四、 胰島素抗性指標 HOMA-IR index............................. 88 五、 肝臟脂質分析..............................................89 六、 腹部脂肪組織及肝臟細胞激素相關基因表現量..................89 七、 脾臟細胞細胞激素分泌量....................................90 八、 脾臟細胞增生反應..........................................90 九、 血清中總 IgG、IgA與IgM抗體含量......................... 90 十、 腹腔細胞細胞激素分泌量................................... 90 十一、循環血液中吞噬細胞之吞噬能力.............................91 十二、腸繫膜淋巴結細胞激素分泌量...............................91 十三、皮耶氏體細胞激素分泌量...................................91 第四節 討論...................................................... 111 第五章、綜合討論與總結論 第一節 綜合討論..................................................117 第二節 總結論....................................................125 第六章、參考文獻................................................. 126 | |
| dc.language.iso | zh-TW | |
| dc.subject | Th1免疫反應 | zh_TW |
| dc.subject | 胰島素抗性 | zh_TW |
| dc.subject | 發炎反應 | zh_TW |
| dc.subject | M2 巨噬細胞 | zh_TW |
| dc.subject | Th1 immune response | en |
| dc.subject | M2 macrophage | en |
| dc.subject | insulin resistance | en |
| dc.subject | inflammation | en |
| dc.title | 影響3T3-L1細胞株分泌細胞激素之食材對高油飲食小鼠的免疫調節作用 | zh_TW |
| dc.title | Effects of dietary factors on cytokines profile of 3T3-L1 cell and their in vivo effects on immune regulatory in mice fed high-fat diet | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃青真(Ching jang Huang),黃慶璨(Ching-Tsan Huang),許瑞祥(Ruey-Shyang Hseu),江伯倫(Bor-Luen Chiang) | |
| dc.subject.keyword | 胰島素抗性,發炎反應,M2 巨噬細胞,Th1免疫反應, | zh_TW |
| dc.subject.keyword | insulin resistance,inflammation,M2 macrophage,Th1 immune response, | en |
| dc.relation.page | 144 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2009-08-18 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
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