請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43527完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李嗣涔(Si-Chen Lee) | |
| dc.contributor.author | Chia-Ming Liang | en |
| dc.contributor.author | 梁家銘 | zh_TW |
| dc.date.accessioned | 2021-06-15T02:22:53Z | - |
| dc.date.available | 2009-08-20 | |
| dc.date.copyright | 2009-08-20 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-08-18 | |
| dc.identifier.citation | [1] Gollnick, SO; Owczarczak, B; Maier, P. LASERS IN SURGERY AND MEDICINE , 38, 509-515 (2006)
[2] Wan-Ping Hu, Jeh-Jeng Wang, Chia-Li Yu, Cheng-Che E Lan, Gow-Shing Chen and Hsin-Su Yu. Journal of Investigative Dermatology, 127, 2048–2057 (2007). [3] Andrei P. Sommer, Antonio L. B. Pinheiro, Adam R. Mester, Ralf-Peter Franke, Harry T. Whelan. Journal of Clinical Laser Medicine & Surgery, 19, 29-33 (2001). [4] Boumedine, R. S. & Roy, D. C. Cytotherapy 7, 134–143 (2005). [5] Liang, HL; Whelan, HT; Eells, JT, et al. NEUROSCIENCE, 139, 639-649 (2006) [6] Elke M. Vinck, Barbara J. Cagnie, Maria J. Cornelissen, Heidi A. Declercq and Dirk C. Cambier. Lasers in Medical Science, 18, 95-99 (2003) [7] Desmet KD, Paz DA, Corry JJ, Eells JT, et al. PHOTOMEDICINE AND LASER SURGERY, 24, 121-128 (2006) [8] Chi-Feng Chen. Effect of Narrow Bandwidth Infrared Radiation on Mungbean Growth and Gene Expression (2007) [9] R. H. Ritchie, Phys. Rev. 106, 874-881 (1957) [10] H. Raether, Surface Plasmons (Springer-Verlag, Berlin, 1988) [11] C. J. Powell, J. B. Swan, Phys. Rev. 118, 640 (1960). [12] William L. Barnes, Alain Dereux and Thomas W. Ebbesen, Nature (London) 424, 824 (2003). [13] M.A. Ordal, L. L. Long, R. J. Bell, S. E. Bell, R. R. Bell, R. W. Alexander, Jr. ,and C. A. Ward, Applied Optics, 22, 1099 (1983). [14] E. Krestchmann and H. Reather, Z.Naturforsch. A 23,2135-2136 (1968) [15] Handbook of Instrumental Techniques for Analytical Chemistry, Ch. 15, edited by C. P. Sherman Hsu. [16] M. V. Trushin. Microbiology, 71 383-385 (2002) [17] Daisuke Hamanaka, Toshitaka Uchino, Naomi Furuse, Wenxue Han, Shun-ichiro Tanaka. International Journal of Food Microbiology 108, 281–285 (2006) [18] Vermeulen, N; Keeler, WJ; Nandakumar, K, et al. BIOTECHNOLOGY AND BIOENGINEERING, 99, 550-556 (2008) [19] C. Lage , P.C.N. Teixeira, A.C. Leita˜o. International Journal of Food Microbiology 108, 281–285 (2006) [20] William Sealy Gosset. Student. (1908) [21] Bradford, M. M. Biochem. 72,248-254 (1976) [22] Handbook of 2-D Electrophoresis Principles and Methods. GE Healthcare [23] Arora PS, Yamagiwa H, Srivastava A, Bolander ME, Sarkar G. (2005) [24] Tanaka, K.; Waki, H.; Ido, Y.; Akita, S.; Yoshida, Y.; Yoshida, T. (1988) [25] Beavis RC, Chait BT (1989) [26] Yi-Tsung Chang, Yi-Ting Wu, Jeng-Han Lee, Chia-Ming Liang, Chao-Ju Huang and Si-Chen Lee. Intensity Dependence of (1,0) and (1,1) Ag/SiO2 Surface Plasmons in Ag/SiO2/Ag Plasmonic Thermal Emitter on Energy Distribution of a Graybody Emitter. [27] Bill Geroge, Peter Mclntyre原著,翁瑞裕編譯.紅外線光譜分析法,高立圖書有限公司, p.198, p.233~237(2001) | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43527 | - |
| dc.description.abstract | 本研究的目的，是設計一種紅外線發射器，利用其所發出的窄頻寬的紅外線去照射大腸桿菌24小時，找出某特定波段的紅外光對細胞發育及蛋白質表現之影響。本研究使用的紅外光發射器，是依據表面電漿子原理製作，此紅外光源結構是在矽基板上鍍上鉬金屬，再鍍上銀/二氧化矽/銀三層薄膜，而最上層的銀製作週期排列的孔洞，藉由鉬金屬通電流加熱，此結構便會發出窄頻寬高功率的紅外光；設計出發射波長為3, 3.5, 4, 4.5, 5μm，半高寬最窄可達0.5μm，加熱到300oC時發射功率可達165mW/cm2。將大腸桿菌經過不同波長之紅外光24小時的照射後，測量其細胞菌落直徑以了解生長狀態，再進而使用蛋白質二維電泳分析法分析其表現蛋白質表現，並利用質譜分析出有差異性的蛋白質。實驗結果顯示大腸桿菌在波長4, 4.5, 5μm 的照射下，會有促進生長的情形。而在紅外光24小時的照射後，其蛋白質表現都會有所不同，當紅外光有促進細胞生長時，其蛋白質有促進的調控表現。反之當細胞沒有生長差異時，其蛋白質則有抑制的調控表現。 | zh_TW |
| dc.description.abstract | The purpose of this study is to design an infrared emitter, and to utilize its narrow bandwidth infrared radiation to irradiate Escherichia coli for 24 hours, in order to identify the specific waveband of infrared radiation which may affect the cell development of Escherichia coli. Infrared radiation emitters used in this study are fabricated based on the principle of surface plasmon. The heat is generated by sending electric current to the molybdenum film on silicon substrate. The infrared source can be achieved by heating the triple layer structure which consists of a SiO2 layer between two Ag films on a silicon substrate. The emission wavelength can be altered by changing the lattice constant and diameter of the hole arrays. In this study, plasmonic thermal emitters with different emission peak wavelengths, i.e., 3.1, 3.5, 4.0, 4.5, and 5.0 μm, were designed and fabricated. The smallest full width at half maximum (FWHM) could be shrunk down to 0.5μm. The highest emitting power can reach 165mW/cm2 at a temperature of 300oC. After 24 hours exposure to infrared radiation with different wavelength, the cell colony diameters of Escherichia coli are measured to understand the cell growth. Two dimensional protein gel electrophoresis and mass spectrometry are also applied to observe the protein expressions pattern. From the results, the cell growth under infrared radiation with peak wavelength at 4, 4.5, 5μm is enhanced. The infrared radiation will also affect the protein expressions. When the cell growth is enhanced by the infrared radiation, its protein expressions are up-regulated. On the contrary, if the cell growth is no significant changed, its protein expressions are down-regulated. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T02:22:53Z (GMT). No. of bitstreams: 1 ntu-98-R96945027-1.pdf: 3670810 bytes, checksum: 0728beddaad11071b076afcb55455200 (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | Content
誌謝 i 摘要 II Abstract III Content V List of Figures IX List of Tables XVI Chapter 1 Introduction 1 1.1 Photobiomodulation 1 1.2 Purpose of this research 1 1.3 Framework of the thesis 5 Chapter 2 The Fundamentals of Plasmonic Thermal Emitters 7 2.1 The fundamentals of surface plasmons 7 2.1.1 Surface plasmons on smooth surfaces 7 2.1.2 Surface Plasmons on the surface with hole arrays 13 2.2 Process flow 17 2.2.1 Fabrication processes of metal hole arrays 17 2.2.2 Fabrication processes of plasmonic thermal emitter 19 2.3 Measuring systems 22 2.3.1 Introduction of FTIR 22 2.3.2 Thermal emitter chamber 25 3.1 E. coli material and experimental setup 27 3.1.1 E. coli material 27 3.1.2 Experimental setup 27 3.2 E. coli Experiment flow 34 3.3 Student’s T-test 36 3.4 Protein expression pattern analysis 38 3.4.1 Protein extraction and Protein assay 38 3.4.2 Two dimensional protein gel electrophoresis analysis 39 3.4.3 Matrix-assisted laser desorption inoization-time of flight mass spectrometry 40 3.5 The effect of temperature 44 Chapter 4 Results and Discussion 46 4.1Infrared radiation experiment #1 (λp= 5.0 μm) 46 4.1.1 Cell growth 46 4.1.2 Protein expression 47 4.2Infrared radiation experiment #2 (λp=4.5 μm) 56 4.2.1 Cell growth 56 4.2.2 Protein expression 57 4.3Infrared radiation experiment #3 (λp=4.0 μm) 66 4.3.1 Cell growth 66 4.3.2 Protein expression 67 4.4Infrared radiation experiment #4 (λp=3.5 μm) 75 4.4.1 Cell growth 75 4.4.2 Protein expression 76 4.5 Infrared exposure experiment #5 (λp= 3.0 μm) 81 4.5.1 Cell growth 81 4.5.2 Protein expression 82 4.6 Infrared radiation effect on agar 90 4.7 The absorption spectra of E. coli 97 4.8 Discussion 101 Chapter 5 Conclusions 103 Reference 104 Appendix I – Protein extraction 107 Appendix II – Two dimensional gel electrophoresis (2DE) 108 Appendix III – In-gel digestion 109 Appendix IV- T-test results of experiment #1 112 Appendix V - T-test results of experiment #2 114 Appendix VI - T-test results of experiment #3 116 Appendix VII - T-test results of experiment #4 118 Appendix VIII - T-test results of experiment #5 120 | |
| dc.language.iso | en | |
| dc.subject | 大腸桿菌 | zh_TW |
| dc.subject | 表面電漿子 | zh_TW |
| dc.subject | 紅外線 | zh_TW |
| dc.subject | E. coli | en |
| dc.subject | surface plasma | en |
| dc.subject | infrared | en |
| dc.title | 窄頻寬紅外線照射對大腸桿菌發育及蛋白質表現之影響 | zh_TW |
| dc.title | Effect of Narrow Bandwidth Infrared Radiation on Escherichia coli Growth and Protein Expression | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 阮雪芬(Hsueh-Fen Juan),管傑雄(Chieh-Hsiung Kuan),林致廷(Chih-Ting Lin),呂學士(Shey-Shi Lu) | |
| dc.subject.keyword | 表面電漿子,紅外線,大腸桿菌, | zh_TW |
| dc.subject.keyword | surface plasma,infrared,E. coli, | en |
| dc.relation.page | 121 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2009-08-18 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
| 顯示於系所單位： | 生醫電子與資訊學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-98-1.pdf 未授權公開取用 | 3.58 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。