請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43526
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 徐源泰 | |
dc.contributor.author | Yan-Huey Chen | en |
dc.contributor.author | 陳彥惠 | zh_TW |
dc.date.accessioned | 2021-06-15T02:22:52Z | - |
dc.date.available | 2012-08-20 | |
dc.date.copyright | 2009-08-20 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-08-18 | |
dc.identifier.citation | 1. 徐源泰. 2005. 杜鵑花屬植物小分子熱休克蛋白與其地理分佈之研究. 內政部營建署太魯閣國家公園管理處委託研究報告.
2. 張永達. 2003. 金門濕地及水韭之分類與生態調查研究. 內政部營建署金門國家公園管理處. 3. 陳進明、王晶苑、劉星、張彥文、王青峰. 2004. 中華水韭遺傳多樣性的RAPD分析. 生物多樣性. 12:348-353. 4. 陳進明、劉星、王青峰. 2005. 中國珍稀瀕危特有蕨類植物—雲貴水韭的遺傳多樣性. 武漢大學學報(理學版). 51:767-770. 5. 郭章儀. 2003. 金門地區水韭之形態與生態調查研究. 國立臺灣師範大學碩士論文. 6. 黃淑芳. 1987. 臺灣水韭的胚胎發育. 國立臺灣大學博士論文. 7. 黃鈞蕙、陳西村、賴明洲、張永達. 2001. 金門國家公園水韭棲地及其形態之初探,植物多樣性-從基因到生態系研討會. 中華植物學會. p66(論文摘要). 8. 黃鈞蕙. 2002. 臺灣水韭棲地之生態因子及其族群遺傳之研究. 國立臺灣師範大學碩士論文. 9. 黃增泉、江蔡淑華、陳尊賢、黃淑芳、楊國禎、陳香君. 1988. 夢幻湖植物生態系之調查研究. 內政部營建署陽明山國家公園管理處. 10. 劉育姍. 2007. 柑橘屬植物親緣關係及分子演化之研究. 國立臺灣大學博士論文. 11. 劉棠瑞,1981. 壹、植物分類學之基礎與方法. 植物分類學. 國立編譯館出版. p.1-86. 12. 謝育慈. 2004. 東亞地區水韭屬植物親緣關係研究. 國立臺灣師範大學碩士論文. 13. Baker, J. G. 1880. A synopsis of the species of Isoetes. J. Bot. 18:65-70. 14. Britton, D. M. and D. F. Brunton. 1991. The Spore and Affinities of Isoetes Taiwanensis. Fern Gaz. 14:73-81. 15. Brown, A. H. D. 1979. Enzyme polymorphism in plant populations. Theoret. Pop. Biol. 15:1-42. 16. Burke, J. J., P. J. O’Mahoney, and M. J. Oliver. 2000. Isolation of Arabidopsis mutants lacking components of acquired thermotolerance. Plant Physiol. 123: 575–588. 17. Campbell, C. S., M. F. Wojciechowski, B. G. Baldwin, L. A. Alice, and M. J. Donoghue. 1997. Persistent nuclear ribosomal DNA sequence polymorphism in the Amelanchier agamic complex)Rosaceae). Mol. biol. evol. 14:81-90. 18. Caplen, C. A. and C. R. Werth. 2000. Isozymes of the Isoetes riparia Complex, I. Genetic variation and relatedness of diploid species. Syst. Bot. 25:235-259. 19. Chang, H. J. and K. S. Hsu. 1977. Isoetes taiwanensis DeVol and its associates. Chinese For. 10:138-142. 20. Chen, J. M., J. Y. Wang, X. Liu, W. R. Gituru, and Q. F. Wang. 2005. RAPD analysis for genetic variation within the endangered quillwort Isoetes hypsophila)Isoetaceae). WUJUS. 10:455-459. 21. Clapham, A. R., T. G. Tutin, and D. M. Moore. 1987. Flora of the British Isles. Cambridge. p.3. 22. Crawford, D. J. 1983. Phylogenetic and systematic inferences from electrophoretic strdies. Elsevier Amsterdam. p257-287. 23. Crawford, D. J. 1985. Electrophoretic data and plant speciaton. Syst. Bot. 10:405-416. 24. DeVol, C. E. 1972. Isoetes found on Taiwan. Taiwania. 17:1-7. 25. Douzery, E. J. P., A. M. Pridgeon, P. Kores, H. P. Linder, H. Kurzwell, and M. W. Chase. 1999. Molecular phylogenetics of Diseae)Orchidaceae): a contribution from nuclear ribosomal ITS sequence. Am. J. Bot. 86:887-899. 26. Duff, R. J. and A. M. Evans. 1992. Allozyme electrophoresis and the taxonomy of two species of Isoetes in the southeastern Appalachians. Am. Fern J. 82:129-141. 27. Flahaut, S., A. Benachour, J. C. Giard, P. Boutibonnes, and Y. Auffray. 1996. Defense against lethal treatments and de novo protein synthesis induced by NaCl in Enterococcus faecalis ATCC 19433. Arch Microbiol. 165:317-324. 28. Gacia, E. and E. Ballesteros. 1998. Effects of building up a dam in a shallow mountain lake)Baciver, central Pyrenees, Spain). Oecology and Aquatany. 11:55-66. 29. Gacia, E., E. Ballesteros, L. Camarero, O. Delgado, A. Palau, R. J. Lluis, and J. Catalan. 1994. Macrophyte form lakes in the eastern Pyrenees: community composition and ordination in relation to environmental factors. Freshwat. Boil. 32:73-81. 30. Ge, S., T. Sang, B. R. Lu, and D. Y. Hong. 1999. Phylogeny of rice genomes with emphasis on orgins of allotetraploid species. Proc. Natl. Acad. Sci. U. S. A. 96:14400-14405. 31. George, H. M. 1955. Taxonomy of vascular plants. The Macmillan Company. New York. p.335, p.340. 32. Golenverg, E. M., M. T. Clegg, M. L. Durbin, J. Doebley, and D. P. Ma. 1993. Evolution of a noncoding region of the chloroplast genome. Mol. Phyl. Evol. 2:52-64. 33. Gottlieb, L. D. 1981. Electrophoretic evidence and plant populations. Prog. Phytochem. 7:1-46. 34. Gottlieb, L. D. 1982. Conservation and duplication of isozymes in plants. Science. 216:373-380. 35. Gottlieb, L. D. 1984. Isozyme evidence and problem solving in plant systematics. Plant biosystematics. Academic Press. Orlando. FL. p343-357. 36. Hamrick, J. L. 1982. Plant population genetics. Am. J. Bot. 69:1685-1693. 37. Haufler, C. H. 1987. Electrophoresis is modifying our concepts of evolution in homosporous pteridophytes. Am. j. bot. 74:953-966. 38. Heckathorn, S. A., C. A. Downs, T. D. Sharkely, and J.S. Coleman. 1998. The small, methionine-rich chloroplast heat-shock protein protects photosystem Ⅱ electron transport during heat stress. Plant physiol. 116:439-444. 39. Hickey, R. J. 1986. The early evolutionary and morphological diversity of Isoetes with descriptions of two new Neotropical species. Syst. Botany. 11:309-321. 40. Hickey, R. J., S. I. Guttman, and W. H. Eshbargh. 1989. Evidence for post-translational modification of triose phosphate isomerase)TPI) in Isoetes)Isoetaceae) . Amer. J. Bot. 76:215-221. 41. Hong, S. W. and E. Vierling. 2000. Mutants of Arabidopsis thaliana defective in the acquisition of tolerance to high temperature stress. Proc. Natl. Acad. Sci. U. S. A. 97:4392-4397. 42. Hoot, S. B. and A. W. Douglas. 1998. Phylogeny of the Proteaceae based on atpB and atpB-rbcL spacer region sequences. Aust. Syst. Bot. 11:301-320. 43. Hoot, S. B. and W. C. Taylor. 2001. The utility of nuclear ITS, a LEAFY homolog intron, and chloroplast atpB-rbcL spacer region data in phylogenetic analyses and species delimitation in Isoetes. Am. Fern J. 91:166-177. 44. Hoot, S. B., N. S. Napier, and W. C. Taylor. 2004. Revealing unknown or extinct lineages within Isoetes using DNA sequences from hybrids. Am. J. Bot. 91:899-904. 45. Hoot, S. B., W. C. Taylor, and N. S. Napier. 2006. Phylogeny and biogeography of Isoetes)Isoetaceae) based on nuclear and chloroplast DNA sequence data. Syst. Botany. 31:449-460. 46. Huang, T. C. and H. J. Chen. 1987. The pollen analysis of the Dream Lake, Taipei, Yang Ming Shan National Park. J. Palynol. 23-24:213-216. 47. Huang, S. F. and S. H. T. Chiang. 1986. The development of the female gametophyte in Isoetes taiwanensis DeVol. Taiwania. 31:15-32. 48. Iba, K. 2002. Acclimative response to temperature stress in higher plants: approaches of gene engineering for temperature tolerance. Annual Review of Plant Biology. 53:225-245. 49. Ingram, A. L. and J. J. Doyle. 2003. The origin and evolution of Eragrostistef)Poaceae) and related polyploids: evidence from nuclear waxy and plastid rps16. Am. J. Bot. 90:116-122. 50. Kapoor, M., G. M. Sreenivasan, N. Goel, and J.Lewis. 1990. Development of thermotolerance in Neurospora crassa by heat shock and other stresses eliciting peroxidase induction. J. Bacteriol. 172:2798-2801. 51. Keeley, J. E. 1981. Isoetes howelii: a submerged aquatic CAM plant? Amer. J. Bot. 68:420-424. 52. Keeley, J. E. 1982. Distribution of diurnal acid metabolism in the genus Isoetes. Amer. J. Bot. 69:254-257. 53. Kott, L. S. 1980. The taxonomy and biology of the genus Isoetes L. in northeastern North America. Ph.D. thesis. University of Guelph. 54. Kott, L. s. 1981. Isoetes acadiensis, a new species from eastern North America. Can. J. Bot. 59:2592-2594. 55. Kott, L. S. and D. M. Britton. 1980. Chromosome numbers for Isoetes in northeastern North America. Can. J. Bot. 58:980-984. 56. Kott, L. S., and D. M. Britton. 1982. A Comparatives Study of Spore Germination of Some Isoete Species of Northeastern North America. Can. J. Bot. 60:1679-1687. 57. Larkindale, J., M. Mishkind, M, and E. Vierling. 2005. Plant responses to high temperature. In MA Jenks, PM Hasegawa, eds, Plant Abiotic Stress. Blackwell Scientific Publications, Oxford)in press) 58. Lawrence, S.D., K. Cline, and G. A. Moore. 1997. Chromoplast development in ripening tomato fruit: identification of cDNAs for chromoplast-targeted proteins and characterization of a cDNA encoding a plastid-localized low-molecular-weight heat shock protein. Plant Mol. Biol. 33:483-492. 59. Lee, G. J., A. M. Roseman, H. R. Saibil, E. Vierling. 1997. A small heat shock protein stably binds heat-denatured model substrates and can maintain a substrate in a folding-competent state. Embo. J. 16:659-671. 60. Lee, G. J. and E. Vierling. 2000. A small heat shock protein cooperates with heat shock protein 70 system to reactivate a heat-denatured protein. Plant Physiol. 122:189-198. 61. Liu, H. 2007. Effects of temperature on the megaspores germination of the rare and critically endangered aquatic plant Isoetes sinensis. J. of South-Central University for Nationalities. 26:39-41,46. 62. Liu, H. and Q. F. Wang. 2003. Aseptic culture of spores in the rare and endangered plant - Isoetes sinensis. Plant Physiol. Commun. 39:466. 63. Liu, X., W. R. Gituru, and Q. F. Wang. 2004. Distribution of basic diploid and polyploidy species of Isoetes in East Asia. J. Biogeogr. 31:1239-1250. 64. Löw, D., K. Brändle, L. Nover, and C. Forreiter. 2000. Cytosolic heat-stress proteins Hsp17.7 class I and Hsp17.3 class II of tomato act as molecular chaperons in vivo. Palnta. 211:575-582. 65. Manen, J. F., A. Natali, and F. Ehrendorfer. 1994. Phylogeny of Rubiaceae - Rubieae inferred from the sequence of a cpDNA intergene region. Pl. Syst. Evol. 190:195-211. 66. Massie, M. R., E. M. Lapoczka, K. D. Boggs, K. E. Stine, and G. E. White. 2003. Exposure to the metabolic inhibitor sodium azide induces stress protein expression and thermotolerance in the nematode Caenorhabditis elegans. Cell Stress & Chaperones. 8:1-7. 67. Meng, F. S. 1999. Studies on pleuromeia from piddle triassic along the Yangtze river valley and systematics of Isoatales. ACTA Geoscientia Sinica. 20:215-222. 68. Motelay, M. L. and A. Vendryes. 1882. Monographie des Isoeteae. Actes Soc. Linn. Bordeaux. 36: 309-406. 69. Neta-Sharir, I., T. Isaacson, S. Lurie, and D. Weiss. 2005. Dual role for tomato eat shock protein 21: protecting photosystem II from oxidative stress and promoting color changes during fruit maturation. Plant Cell. 17:1829-1838. 70. Ordon, F., A. Schiemann, and W. Friedt. 1997. Assessment of the genetic relatedness of barley accessions)Hordeum vulgare s. l.) resistant to soil-borne mosaic-inducing viruses)BaMMV, BaYMV, BaYMV-2) using RAPDs. Theor. Appl. Genet. 94: 325-330. 71. Parsell, D. A. and S. Lindquist. 1993. The function of heat-shock proteins in stresstolerance: degradation and reactivation of damaged proteins. Annu. Rev. Genet. 27:437-496. 72. Pfeiffer, N. E. 1922. Monograph of the Isoetaceae. Ann. Missouri Bot. Gard. 9: 79-232. 73. Rieseberg, L. H. and N. C. Ellstrand. 1993. What can molecular and morphologica markers tell us about plant hybridization? Critical Review of Plant Science. 12:213-241. 74. Ritossa, F. 1962. A new puffing pattern induced by temperature shock and DNP in Drosphila. J. Mol. Biol. 18: 571-573. 75. Roelofs, J. G. M., T. E. Brandrud, and A. J. P. Smolders. 1994. Massive expansion of Juncos bulbosus L. after liming of acidified SW Norwegian lake. Aquat. Bot. 48:187-202. 76. Rorslett, B. 1987. Niche statistics of submerged macrophytes in Tyrifjord, a large oligotrophic Norwegian Lake. Archivfuer Hydorbiologie. 111:283-308. 77. Rorslett, B. and S. W. Johansen. 1995. Dynamic response of the submerged macrophytes, Isoetes lacustris, to alternating light levels under field conditions. Aquat. Bot. 51: 223-242. 78. Sang, T., D. Crawford, and T. F. Stuessy. 1995. Documentation of reticulate evolution in Peonies(Paeonia)using sequences of internal transcribed spacer of nuclear ribosomal DNA:implications for biogeography and concerted evolution. Proc. Natl. Acad. Sci. U. S. A. 92:6813-6817. 79. Small, R. L. and J. F. Wendel. 2000. Copy number lability and evolutionary dynamics of the adh gene family in diploid and tetraploid cotton)Gossypium). Genetics. 155:1913-1926. 80. Small, M. and F. J. Hickey. 1997. Levels and patterns of genetic variation in Isoetes Karstenii with observations on I. Palmeri. Am. Fern J. 87:104-115. 81. Srivastava, G. K., D. D. Pant, and P. K. Shukla. 1993. The genus Isoetes L. in India. Am. Fern J. 83:105-119. 82. Sterling, J. S. 1982. A germination method for Isoëtes. Am. Fern J. 72: 61. 83. Sun, W., M. V. Montagu, and N. Verbruggen. 2002. Small heat shock proteins and stress tolerance in plants. Biochim. Biophys. Acta. 1577: 1-9. 84. Taylor, W. C. and R. J. Hickey. 1992. Habitat, evolution, and speciation in Isoetes. Ann. Mo. Bot. Gard. 79: 613-622. 85. Taylor, W. C. and T. L. Neil. 1986. Germinating Spores and Growing Sporelings of Aquatic Isoëtes. Am. Fern J. 76: 21-24. 86. Taylor, W. C., A. R. Lekschas, Q. F. Wang, X. Liu, N. S. Napier, and S. B. Hoot. 2004. Phylogenetic relationships of Isoetes in China as revealed by nucleotide sequences of the second intron of a LEAFY homolog. Am. Fern J. 94:196-205. 87. Tinker, N. A., M. G. Fortin, and D. E. Mather. 1993. Random amplified polymorphic DNA and pedigree relationship in spring barley. Theor. Appl. Genet. 85: 976-984. 88. Tisseres, A. A. and K. Mitchell. 1974. Some new proteins induced by temperature shock in Drosophila. J. Mol. Biol. 84: 389-398. 89. Waters, E. R. 1995. The molecular evolution of the small heat shock protein in plants. Genetics. 141: 785-795. 90. Waters, E. R. 2003. Molecular adaptation and the origin of land plants. Molecular Phylogenetics and Evolution. 29: 456-463. 91. Waters, E. R. and E. Vierling. 1999. Chloroplast small heat shock proteins: evidence for atypical evolution of an organelle-localized protein. Proc. Natl. Acad. Sci. U. S. A. 96: 14394-14399. 92. Welsh, J. and M. McClelland. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18: 7213-7218. 93. Wilde, J., R. Waugh, and W. Powell. 1992. Genetic fingerprinting of Theobroma clones using randomly amplified polymorphic DNA markers. Theor. Appl. Genet. 83: 871-877. 94. Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalsky, and S. V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531-6535. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43526 | - |
dc.description.abstract | 水韭屬植物(Isoetes sp.) 為一起源於古生代之物種,目前臺灣水韭(I. taiwanensis DeVol)為臺灣唯一被命名之水韭,然而1991年時,金門太武山上亦發現水韭之棲地。前人研究中,金門地區水韭與臺灣水韭之各種特徵極為相似,但仍有些許差異性,而以internally transcribed spacers(ITS)區域及LEAFY homolog的第二個intron(LFY)做為分子標誌,均未能對金門地區水韭之身分做一良好解說;且金門之水韭棲地範圍狹小,水位淺且易乾涸,水韭數量甚少。本研究中,以RAPD方法分析所得結果,發現金門地區水韭與臺灣水韭之相似度僅31.42%,與寬葉水韭(I. japonica)相似度為34.32%,而以聚合酶連鎖反應擴增臺灣水韭、金門地區水韭及寬葉水韭之小分子熱休克蛋白sHSP21基因保守序列部分片段,得其長度為410 bp,結果發現金門地區水韭與臺灣水韭兩者基因序列相似度達96.7%,與寬葉水韭相似度則為90.9%,闡釋金門地區水韭應與臺灣水韭有所差異。為解釋何以金門地區水韭能生存於太武山上叫嚴苛之環境,故模擬金門地區夏季溫度變化處理金門地區水韭與臺灣水韭,並分析其sHSP21 mRNA之表現量,結果發現金門水韭在育到高溫刺激時,能快速表達sHSP21 mRNA,臺灣水韭則表達較慢,顯示金門地區水韭能快速適應高溫的環境。此外,本實驗中亦以不同溫度處理金門地區水韭與臺灣水韭之大、小孢子不同天數,觀察溫度變化對於孢子萌發之情形,期望對於日後水韭復育有所幫助,結果金門地區水韭以5℃低溫處理過之大孢子萌發率較高,臺灣水韭則是以35℃處理後之大孢子萌發率較高,顯示金門地區水韭與臺灣水韭孢子萌發之生理狀況甚異。然而,以RAPD分析金門太武山水韭棲地內其水韭之遺傳多樣性,發現其PPB僅18.82 %,金門地區水韭有瀕臨滅絕之危機。 | zh_TW |
dc.description.abstract | Isoetes sp. is an ancient plant since Late Devonia. There only one I. sp. had been named I. taiwanensis DeVol in Taiwan. However, there was found another habitat of I. sp. at the Taiwu mountain in Kinmen. In the pass research, the characteristics of quillwort in Kinmen are similar to I. taiwanensis, but still have some differences between two of them. And the internally transcribed spacers(ITS) region and the second intron of LEAFY homolog are not proper to be the molecular marker on distinguishing the phylogeny of the quillwort in Kinmen. Besides, the range of habitat in Kinmen is narrow, and the amount of quillwort is few. The aim to discuss the phylogeny of this quillwort, RAPD and small heat shock protein sHSP21 was used in this study. The RAPD analysis also showed only 31.42% similarity between I. taiwanensis and the quillwort in Kinmen. And by amplifying the sHSP21 partial conserved sequence of I. taiwanensis and the quillwort in Kinmen with Polymerase Chain Reaction, it was found 96.7% similarity between them. In addition, the sHSP21 mRNA expression and spore germination as well as temperature response varies between these two samples. And the data in this thesis showed the genetic diversity of the quillwort group at Kinmen habitat is very low and this species of quillwort is endangered. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T02:22:52Z (GMT). No. of bitstreams: 1 ntu-98-R96628215-1.pdf: 5430779 bytes, checksum: 3a006626dc74143df71cccc89037fc67 (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 中文摘要 i
Abstract ii 目錄 iii 圖次 vi 表次 ix 第一章 前言 1 第二章 前人研究 2 第一節 水韭屬植物之介紹 2 第二節 世界水韭屬植物分類概述 4 第三節 近代東亞地區水韭屬植物之分類 9 第四節 植物小分子熱休克蛋白及其於分子標誌研究上之介紹 16 第五節 水韭屬植物孢子胚胎發育 21 第六節 以RAPD方法檢測水韭屬植物居群之遺傳多樣性 24 第三章 材料與方法 26 第一節 研究材料 26 第二節 實驗方法 26 一、 以RAPD 方法進行水韭屬植物親緣關係之分析 26 (一) DNA之製備與純化 26 (二) 聚合酶鏈鎖反應(Polymerase Chain Reaction, PCR)之進行 27 (三) RAPD統計分析方法 27 二、 以小分子熱休克蛋白sHSP21部分基因序列進行水韭屬植物親緣關係之分析 27 (一) 相近物種 sHSP 序列之比對與引子對設計 27 (二) 以sHSP21保守區域設計引子對 27 (三) 以水韭屬植物核苷酸序列設計專一性較高之sHSP21引子對 28 (四) 聚合酶鏈鎖反應(Polymerase Chain Reaction, PCR)之進行 28 (五) TA cloning 28 (六) 遺傳譜系分析 29 三、 臺灣水韭及金門地區水韭小分子熱休克蛋白sHSP21 mRNA表現量分析 29 (一) 水韭屬植物熱刺激之實驗設計 29 (二) RNA之製備與純化 29 (三) 反轉錄聚合酵素鏈鎖反應(Reverse transcription PCR, RT-PCR) 30 (四) 以水韭屬植物核苷酸序列設計即時定量聚合酶連鎖反應專一性之sHSP21引子對 30 (五) 即時定量聚合酶連鎖反應 30 四、 臺灣水韭及金門地區水韭受熱刺激後之葉綠素螢光測定析 31 五、 臺灣水韭及金門地區水韭之孢子萌芽試驗 31 六、 金門地區水韭之遺傳多樣性調查 31 第四章 結果與討論 35 第一節 金門地區水韭棲地觀察及樣本採集 35 第二節 水韭屬植物親緣關係分析 43 一、 以RAPD方法進行水韭屬植物親緣關係之分析 43 二、 相近物種之小分子熱休克蛋白核酸序列設計引子對擴增水韭基因片段 49 三、 水韭小分子熱休克蛋白sHSP21基因之比較 49 第三節 臺灣水韭及金門地區水韭受熱刺激後,其小分子熱休克蛋白sHSP21 mRNA表現量分析及葉綠素螢光偵測分析 57 第四節 臺灣水韭及金門地區水韭之孢子萌芽試驗 61 第五節 金門地區水韭之遺傳多樣性調查 70 第五章 結論 86 參考文獻 88 附錄一、不同植物之chloroplast sHSP 胺基酸序列 98 附錄二、不同植物之 ER sHSP 胺基酸序列 99 附錄三、不同植物之 cytosolic classI sHSP 胺基酸序列 100 附錄四、不同植物之 mitochondrion sHSP 胺基酸序列 101 附錄五、不同類小分子熱休克蛋白胺基酸序列保守區域所設計之引子 102 | |
dc.language.iso | zh-TW | |
dc.title | 臺灣水韭屬植物親緣關係與其環境適應之研究 | zh_TW |
dc.title | Study of Phylogenetic Relationship and Environmental Adaption in the Genus Isoetes in Taiwan | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 張育森 | |
dc.contributor.oralexamcommittee | 許輔,曾文聖 | |
dc.subject.keyword | 分子標誌,小分子熱休克蛋白,遺傳多樣性,逢機擴增多型性DNA, | zh_TW |
dc.subject.keyword | molecular maker,genetic diversity,Random Amplified Polymorphic DNA(RAPD), | en |
dc.relation.page | 102 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2009-08-18 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 園藝學研究所 | zh_TW |
顯示於系所單位: | 園藝暨景觀學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-98-1.pdf 目前未授權公開取用 | 5.3 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。