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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳進庭(Chin-Tin Chen) | |
dc.contributor.author | YA-I Yang | en |
dc.contributor.author | 楊雅怡 | zh_TW |
dc.date.accessioned | 2021-06-15T01:53:54Z | - |
dc.date.available | 2010-07-03 | |
dc.date.copyright | 2009-07-03 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-06-30 | |
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Diagnostic microbiology and infectious disease. 45, 73-76 37 Anderegg, T. R. and Jones, R. N. (2004) Preliminary susceptibility testing guidelines for AZD2563, a long-acting oxazolidinone. International journal of antimicrobial agents. 23, 6-10 38 Holmberg, A., Lood, R., Morgelin, M., Soderquist, B., Holst, E., Collin, M., Christensson, B. and Rasmussen, M. (2009) Biofilm formation by Propionibacterium acnes is a characteristic of invasive isolates. Clin Microbiol Infect 39 Alipour, M., Halwani, M., Omri, A. and Suntres, Z. E. (2008) Antimicrobial effectiveness of liposomal polymyxin B against resistant Gram-negative bacterial strains. International journal of pharmaceutics. 355, 293-298 40 Burwen, S. J. and Satir, B. H. (1977) A freeze-fracture study of early membrane events during mast cell secretion. The Journal of cell biology. 73, 660-671 41 Nicolau, D. P. (2008) Carbapenems: a potent class of antibiotics. Expert opinion on pharmacotherapy. 9, 23-37 42 Vaara, M. and Vaara, T. 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(1996) Multidrug efflux pumps of gram-negative bacteria. Journal of bacteriology. 178, 5853-5859 52 王澤嫻。2008。以微脂體或是微胞包埋血紫質之光動力殺菌探討。國立台灣 大學,微生物與生化學研究所碩士論文。 53 蔣采昕。2007。細菌細胞壁組成對光動力治療抑制革蘭氏陽性菌與革蘭氏陰性菌效果之影響。國立台灣大學,微生物與生化學研究所碩士論文。 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43392 | - |
dc.description.abstract | 臨床上,目前普遍使用抗生素治療因革蘭氏陰性菌所引起的感染症,這樣的治療方法缺點在於抗生素的濫用帶來的篩選壓力,使得抗藥性細菌出現。革蘭氏陰性菌具有多重的抗藥性機制,一旦發現抗藥性菌株大多已是多重抗藥性菌株,造成後續治療上的困難。本實驗室先前研究發現,CX能有效加強光動力的殺菌效果,希望藉由CX的協助加強抗生素對革蘭氏陰性菌的殺菌效果,降低抗生素的使用量。
研究發現,CX和抗生素作用與抗生素單獨使用時相比較,發現能多降低7個log值生菌數而達全殺的效果,而且加入CX於不同抗生素的MIC濃度時,最終達到的殺菌效果顯著比使用MIC以上濃度的殺菌效果更好,因此CX可加強不同種類的抗生素對革蘭氏陰性菌之殺菌效果,同時亦能協助降低抗生素使用劑量;CX的加強殺菌效果也對抗藥性菌株有效,但CX無法加強EDTA在綠膿桿菌的殺菌效果。至於應用在綠膿桿菌的生物膜殺菌上,可能是受到胞外聚合物的阻礙使CX無法進入生物膜與細菌接觸,因此無法如同在懸浮培養上有增強殺菌的效果。未來實驗室計劃將朝向改良CX的性質與破壞生物膜表面的胞外聚合物,再觀察CX在抗生素對生物膜上的殺菌效果之影響 | zh_TW |
dc.description.abstract | Antibiotics are the most popular antimicrobial agent used for the treatment of infection caused by Gram-negative bacteria. However, drug-resistance is a growing problem largely due to the widespread use of antibiotics in medicine. The opportunistic Pseudomonas aeruginosa (P. aeruginosa) is one of Gram-negative bacteria, which has been identified several drug-resistant strains in clinics. Therefore, it is necessary to develop an alternative antimicrobial technique which is effective in the treatment of multi-drug resistant bacteria infections.
In the presence of compound X (CX), we have previously found that hematoporphyrin (Hp)-mediated photodynamic inactivation (PDI) could increase the bacterial killing against Gram-positive as well as Gram-negative bacteria. In this study, we further address whether CX can exert synergistic antimicrobial effect in antibiotics against Gram-negative bacteria. We found that CX can synergistically increase the antibiotics-mediated bactericidal effects. Under the MIC concentration, the bacterial effect of Polymyxin B 、 Colistin 、 Imipenem 、Gentamicin and Ciprofloxacin is around 2~3 log against P. aeruginosa in platonic culture; however, complete killing could be found in the presence of 0.25% CX. Similar results were also found in other Gram-negative bacterial, Acinetobacter baumannii。However, this synergistic effect of antibiotics and CX was not found in biofilm study, which might relate to the extracellular polymeric substances of biofilm. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T01:53:54Z (GMT). No. of bitstreams: 1 ntu-98-R96b47410-1.pdf: 1979685 bytes, checksum: 05b465e4fd723f0586af314653059fec (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 目 錄
第一章 序論 1 1. 人類與微生物 1 1.1微生物對人類的益處與害處 1 1.2常見的細菌感染 2 1.2.1造成感染的細菌簡介以及所引起的疾病 2 2. 生物膜 3 2.1 生物膜的定義 3 2.2 生物膜的形成 3 2.3 生物膜的結構 4 2.4 生物膜與人類的關係 5 2.5 生物膜的抗藥性機轉 5 3. 抗生素治療疾病簡史與治療上的瓶頸 7 3.1抗生素發展史與分類 7 3.2抗生素抑菌效果的評估方法 9 3.3目前在使用抗生素治療上的瓶頸 10 3.4目前改進抗生素治療的方法 11 4. 非抗生素物質在抑菌上的應用 11 4.1 目前常見具有抑菌功能之物質 11 4.2 本論文中使用的具有抑菌功能之物質 12 5. 實驗動機與目的 13 第二章 材料與方法 14 2.1藥品與儀器 14 2.2菌種來源與保存方法 15 2.2.1菌種來源 15 2.2.2菌種保存與活化 15 2.3實驗方法 16 2.3.1抗生素stock配製 16 2.3.2 CX stock配製 16 2.3.3抗生素的敏感性分析 16 2.3.4單獨CX殺菌效果測試 17 2.3.5綠膿桿菌之EDTA-spheroplasts製備 17 2.3.6 CX結合抗生素殺菌 17 2.3.7 CX結合EDTA殺菌 18 2.3.8 CX結合抗生素對抗藥性菌株之殺菌 18 2.3.9 Biofilm培養 18 2.3.10 CX結合抗生素於生物膜上之殺菌 18 第三章 實驗結果 20 【第一部分:CX在wild type懸浮菌液中的殺菌效果的探討】 20 3.1抗生素對革蘭氏陰性菌代表菌株綠膿桿菌的殺菌效果分析 20 3.2不同濃度CX在PMB對綠膿桿菌殺菌效果的影響 21 3.3 0.25% CX對不同濃度PMB殺菌效果的影響 21 3.4顯著降低生菌數的現象與單獨CX作用的影響之關係 22 3.5 0.25% CX對EDTA殺菌效果的影響 22 3.6 CX能加強PMB殺菌效果於其他革蘭氏陰性菌上之應用 23 3.7 CX的加強殺菌效果於不同功能的抗生素之應用 23 3.7.1 CX對破壞membrane結構的抗生素在革蘭氏陰性菌殺菌效果 23 的影響 3.7.2 CX對抑制細胞壁合成的抗生素在革蘭氏陰性菌殺菌效果的影響 24 3.7.3 CX對抑制蛋白質合成的抗生素在革蘭氏陰性菌殺菌效果的影響 24 3.7.4 CX對抑制核酸合成的抗生素在革蘭氏陰性菌殺菌效果的影響 24 【第二部分:CX對抗藥性菌株殺菌效果的探討】 26 3.8抗藥性菌株的選擇 26 3.8.1 CX在抗生素對CIP resistant的綠膿桿菌的殺菌效果之影響 26 3.8.2 CX在抗生素對CIP intermediate的綠膿桿菌的殺菌效果之 影響 26 【第三部分:CX對處於生物膜形態細菌殺菌效果的探討】 29 3.9 CX在抗生素對處於生物膜形態細菌的殺菌效果之影響 29 3.10較高劑量CX以及水溶性CX對處於生物膜形態細菌的殺 菌效果之影響 29 第四章 討論 29 4.1 CX能加強不同功能抗生素的殺菌效果之探討 29 4.2 CX對加強PMB與EDTA殺菌效果差異上的探討 30 4.3 CX影響抗生素對抗藥性菌株殺菌效果的之探討 30 4.4 CX影響抗生素對細菌生物膜型態的殺菌效果的之探討 31 第五章 結語與未來工作 32 附圖表 33 圖表 41 參考文獻 54 附圖表目次 附表目錄 附表一:人體各部位常見的正常菌叢 33 附表二:體外測試各種方法的優缺點與應用 34 附表三:依抗生素功能分類表 35 附圖目錄 附圖一:Colistin (polymyxin E) and Polymyxin B的結構圖 36 附圖二:生物膜形成示意圖 37 附圖三:生物膜之覃狀結構假想圖 38 附圖四:論文架構圖 39 附圖五:生物膜反應器及裝置 40 結果圖目錄 圖一:抗生素PMB對綠膿桿菌的MIC以及殺菌效果 41 圖二:不同濃度CX以及不同濃度醋酸在PMB對綠膿桿菌殺菌效果 42 的影響 圖三:0.25% CX對不同濃度PMB殺菌效果的影響 43 圖四:單獨CX對不同菌數的綠膿桿菌之殺菌效果影響 44 圖五:0.25% CX對經EDTA處理過的綠膿桿菌之殺菌效果影響 45 圖六:0.25% CX對經PMB處理過的鮑氏不動桿菌殺菌效果的影響 46 圖七:0.25% CX對經抗生素Colistin處理過的 47 綠膿桿菌殺菌效果的影響 圖八:0.25% CX對經抗生素Imipenem處理過的 48 綠膿桿菌殺菌效果的影響 圖九:0.25% CX對經抗生素Gentamicin處 49 理過的綠膿桿菌殺菌效果的影響 圖十:0.25% CX對經抗生素Ciprofloxacin處 50 理過的綠膿桿菌殺菌效果的影響 圖十一:0.25% CX對經CIP處理過的(A)CIP resistant菌株(B) 51 CIP intermediate菌株殺菌效果的影響 圖十二:0.25% CX對綠膿桿菌生物膜殺菌效果的影響 52 圖十三:0. 5% CX、0.25% dCX對處於生物膜形態細菌殺菌效果 53 的影響 | |
dc.language.iso | zh-TW | |
dc.title | CX加強抗生素對革蘭氏陰性菌的殺菌效果之機制探討 | zh_TW |
dc.title | Action Mechanism of CX in Increasing the Bactericidal Effect by Antibiotics on Gram-negative Bacteria | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 鄧麗珍,許瑞祥,黃慶燦,蔡翠敏 | |
dc.subject.keyword | 抗生素,最低抑制濃度,生物膜,革蘭氏陰性菌, | zh_TW |
dc.subject.keyword | Antibiotics,Minimum inhibitory concentration,Biofilm,Gram-negative, | en |
dc.relation.page | 58 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2009-06-30 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
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