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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 黃鵬鵬(Pung-Pung Hwang) | |
dc.contributor.author | Liang-Chun Wu | en |
dc.contributor.author | 吳亮均 | zh_TW |
dc.date.accessioned | 2021-06-15T01:43:24Z | - |
dc.date.available | 2013-08-26 | |
dc.date.copyright | 2011-08-26 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-15 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43220 | - |
dc.description.abstract | 過去研究中有關血管加壓素(AVP)的主要角色是參與調控哺乳類腎臟離子及水分平衡的功能,而AVP的同源基因AVT在魚類生理中調控滲透壓平衡的角色也常被提及,但對於其調節的機制卻所知有限。本實驗目的為利用分子生物學、生化及生理學的方法研究AVT在斑馬魚離子運輸及滲透壓平衡中所扮演的角色及調控機制。實驗中利用RT-PCR的方式檢測AVT於不同胚胎時期及成魚各組織間的表現,並進一步觀察胚胎於低張環境中AVT基因的表現情形。結果顯示AVT於胚胎發育極早期即有表現及廣泛表現於成魚不同組織中並在低張環境中表現增加。此外,以專一的反義核酸 (morpholini oligos) 抑制AVT短肽的合成後,鈉離子及氯離子在胚胎中的含量顯著性地下降,離子運輸蛋白基因表現及富鈉鉀幫浦細胞(Na+-K+-ATPase-riched cell)、富氫幫浦細胞(H+-ATPase-riched cell)的細胞密度皆顯著下降,而與離子細胞分化相關的轉錄因子的基因表現及表皮幹細胞密度等亦顯著地下降。綜合以上所述,AVT參與調控斑馬魚胚胎時期的離子平衡並與離子細胞之分化與增生的調控機制上扮演重要的角色。 | zh_TW |
dc.description.abstract | Arginine vasopressin (AVP) has been found to be involved in ionoregulation and water metabolisms in kidney of mammals. Arginine vasotocin, as the homolog of AVP, were also mentioned frequently in fish physiology but only little was known about the regulatory mechanisms. The purpose of the present study is to explore the role of AVT in ion regulatory mechanisms in zebrafish by using molecular physiological approaches. The mRNA expression were detected by RT-PCR and results showed that AVT gene expressed in very early stage and expressed extensively in different tissues in addition to brain. AVT gene expression was also found to increase in hypoosmotic environment by using qRT-PCR. Besides, the ion content of Cl- and Na+, expression of several ionocyte-related genes, essential transcription factor for ionocyte differentiation, and epidermal stem cell were decreased by knockdown of AVT gene. Taken all together, AVT plays important roles in ion regulation of zebrafish embryo and participates in both cell proliferation and differentiation of ionocyte. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T01:43:24Z (GMT). No. of bitstreams: 1 ntu-100-R98b45008-1.pdf: 7877745 bytes, checksum: c16b4d351900143e9b9bf2353b4a5ff6 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 口試委員會審定書 2
謝辭 3 摘要 4 Abstract 5 Table of contents 6 Introduction 8 Functions of arginine vasotocin 8 Short-term functions of AVT 9 Roles of AVT in cell proliferation and differentiation 10 Roles of AVT in iono- and osmo-regulation 11 Zebrafish as model to study fish in iono- and osmo-regulatory mechanisms 14 Purpose 15 Materials and methods 16 Experimental fish 16 Preparation of total RNA 16 Reverse-transcription polymerase chain reaction (RT-PCR) analysis 17 Translational knockdown with antisense morpholino oligonucleotides (MO) 17 Whole-mount in situ hybridization 18 Whole-mount immunohistochemistry 19 RNA probe synthesis 19 Measurement of whole body ion contents 20 Statistical analysis 20 Results 21 Expression of AVT in zebrafish tissues and embryos 21 Effect of AVT MOs on whole body ion contents in zebrafish embryos 21 Effects of AVT knockdown on ionocyte-related genes expression and ionocyte differentiation 22 Effects of vasotocin knockdown on ionocyte progenitor cell 23 Effects of vasotocin knockdown on epidermal stem cell expressions 23 Discussion 24 The distribution of AVP/AVT 24 The environmental effects on expression of AVP/AVT 25 The role of AVT in osmo- and iono-regulatory mechanisms 26 The roles of AVT in cell differentiation and proliferation 28 Perspective: 30 References 32 Table 43 Fig. 1. RT-PCR analysis of avt transcripts in zebrafish adult tissues and developing zebrafish embyrogenesis. 44 Fig. 2. Effects of the hypoosmotic treatment on mRNA expression of avt in zebrafish embryo. 45 Fig. 3. Effects of the AVT MO on ion content in zebrafish 72 hpf embryos. 46 Fig. 4. Effects of the AVT MO on mRNA expression of ionocyte-related genes in zebrafish embryo. 47 Fig. 5. Effects of the AVT overexpression on cell density of Na+ – K + –ATPase-rich cells (NaRC, red) and H + –ATPase-rich cells (HRC, green) in zebrafish embryos. 48 Fig. 6. Effects of AVT MO on foxi3a expression in zebrafish embryos. 49 Fig. 7. Effects of knockdown of AVT on P63 expression in zebrafish embryos. 50 | |
dc.language.iso | en | |
dc.title | 血管加壓素在斑馬魚離子調控中扮演的角色 | zh_TW |
dc.title | The roles of arginine vasotocin in ionoregulation of zebrafish | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 羅秀婉(Shiu-Wann Lou),張清風(Ching-Fong Chang),林豊益(Li-Yih Lin) | |
dc.subject.keyword | 血管加壓素,斑馬魚,胚胎,離子調控,離子細胞,表皮幹細胞,細胞增生,細胞分化, | zh_TW |
dc.subject.keyword | vasopressin,zebrafish,embryo,ionoregulation,ionocyte,cell differentiation,cell proliferation,epidermal stem cell, | en |
dc.relation.page | 50 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-08-16 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
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