請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/42760完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張上鎮(Shang-Tzen Chang) | |
| dc.contributor.author | Ching-Yu Hsieh | en |
| dc.contributor.author | 謝慶餘 | zh_TW |
| dc.date.accessioned | 2021-06-15T01:22:17Z | - |
| dc.date.available | 2017-07-23 | |
| dc.date.copyright | 2009-07-28 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-07-24 | |
| dc.identifier.citation | 王松永（1983）商用木材。林產事業協會。103-104頁。
李文馨（2008）柳杉樹皮抗細菌及抗氧化活性成分之研究。國立臺灣大學森林環境暨資源學系碩士論文。108頁。 李家璞（2007）苦茶油之木質酚成分、體外抗氧化及體內護肝作用。國立中興大學食品暨應用生物科技學系博士論文。204頁。 吳志鴻、王升陽、張上鎮（2001）本土樹種的研究新契機－抗氧化抽出成分。林產工業。20(3)：251-258。 吳志鴻（2004）相思樹心材具抗氧化及抗發炎成分之分析與鑑定。國立臺灣大學森林環境暨資源學系博士班論文。191頁。 林修賢（2007）相思樹葉子抗氧化活性成分之分析與鑑定。國立臺灣大學森林環境暨資源學系碩士論文。120頁。 邱年永、張光雄（1986）原色臺灣藥用植物圖鑑。南天。287頁。 陳正和（2002）臺灣最普遍的森林喬木之一－相思樹。臺灣林業。28(6): 59-61。 黃志煜（2006）相思樹花之抗氧化活性成分分離與鑑定。國立臺灣大學森林環境暨資源學系碩士論文。91頁。 童鈺棠、吳志鴻、張上鎮（2005）相思樹枝條抽出物抗氧化活性之探討。中華林學季刊 38（3）: 367-375。 楊鎮瑋（2007）臺灣產忍冬屬植物生物活性探索。國立中興大學森林學研究所碩士論文。101頁。 劉棠瑞（1960）臺灣木本植物圖誌第一册。國立臺灣大學農業學院叢書，第8種；林學叢書，第1種。台北。484頁。 鄭森松（2006）柳杉抗真菌及抗蟲成分之分析與鑑定。國立臺灣大學森林學系暨資源學系博士論文。204頁。 應紹舜（1987）臺灣高等植物彩色圖誌第二卷。118頁。 Adewusi, S. R., O. S. Falade, and C. Harwood (2003) Chemical composition of Acacia colei and Acacia tumida seeds-potential food source in the semi-arid tropics. Food Chem. 80: 187-195. Adiga, U., V. D'Souza, A. Kamath, and N. Mangalore (2007) Antioxidant activity and lipid peroxidation in preeclampsia. J. Chin. Med. Assoc. 70 (10): 435-438. Alam, Z. I., A. Jenner, S. E. Daniel, A. J. Lees, N. Cairns, C. D. Marsden, P. Jenner, and Halliwell, B. (1997) Oxidative DNA damage in the parkinsonian brain: an apparent selective increase in 8-hydroxyguanine levels in Substantia Nigra. J. Neurochem. 69(3): 1196-1203. Ahmed, F. N., F. N. Naqvi, and F. Shafiq (2006) Lipid peroxidation and serum antioxidant enzymes in patients with type 2 diabetes mellitus. Ann. N Y Acad. Sci. 1084: 481-489. Aruoma, O. I. (1994) Nutrition and health aspects of free radicals and antioxidants. Food Chem. Toxicol. 32(7): 671-683. Aruoma, O. I. (1998) Free radicals, oxidative stress, and antioxidants in human health and disease. JAOCS (75): 2. Ban, J. Y., S. Y. Jeon, K. Bae, K. S. Song, and Y. H. Seong (2006) Catechin and epicatechin from Smilacis chinae rhizome protect cultured rat cortical neurons against amyloid β protein (25-35)-induced neurotoxicity through inhibition of cytosolic calcium elevation. Life Sci. 79: 2251-2259. Benavides, A., P. Montoro, C. Bassarello, S. Piacente, and C. Pizza (2006) Catechin derivatives in Jatropha macrantha stems: characterisation and LC/ESI/MS/MS quali-quantitative analysis. J. Pharm. Biomed. Anal. 40(3): 639-647. Bharath, M. M. S., S. Ramesh, N. R. Chandra, and M. R. S. Rao (2002) Identification of a 34 amino acid stretch within the c-terminus of histone H1 as the DNA-condensing domain by site-directed mutagenesis. Biochem. 41(24): 7617-7627. Burnett, B. P., Q. Jia, Y. Zhao, and R. M. Levy (2007) A medicinal extract of Scutellaria baicalensis and Acacia catechu acts as a dual inhibitor of cyclooxygenase and 5-lipoxygenase to reduce inflammation. J. Med. Food. 10 (3): 442-451. Chae, H. Z., S. W. Kang, and S. G. Rhee (1999). Isoforms of mammalian peroxiredoxin that reduce peroxides in presence of thioredoxin. Methods Enzymol. 300: 219-226. Chang, S. T., J. H. Wu, S. Y. Wang, P. L. Kang, N. S. Yang, and L. F. Shyur (2001) Antioxidant activity of extracts from Acacia confusa bark and heartwood. J. Agric. Food Chem. 49: 3420-3423. Chung, S. K., Y. C. Kim, Y. Takaya, K. Twrashima, and M. Niwa (2004) Novel flavonol glycoside, 7-O-methyl mearnsitrin, from Sageretia theezans and its antioxidant effect. J. Agric. Food Chem. 52: 4664-4668. Cos, P., L. Ynig, M. Calomme, J. P. Hu, K. Cimanga, B. P. Van, L. Pieters, A. J. Vlietinck, and D. B. Vanden (1998) Structure-activity relationship and classification of flavonoids as inhibitors of xanthine oxidase and superoxide scavengers. J. Nat. Prod. 61: 71-76. Dinis, T. C. P., V. M. C. Madeira, and L. M. Almeida (1994) Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosaliculate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch. Biochem. Biophys. 315: 161-169. Duh, P. D., Y. Y. Tu, and G. C. Yen (1999) Antioxidant activity of water extract of Harng Jyur (Chrysanthemum morifolium Ramat). Lebensm. Wiss. U. Technol. 32: 269-277. Farnsworth, N. R. (1990) The role of ethnopharmacology in drug development. Bioactive Compounds from Plants. CIBA Foundation Symposium #154. Wiley, Bangkok, pp. 2–11. Field, M., C. G. Lewis, and M. D. Lura (1996) Allopurinol, an inhibitor of xanthine oxdiase, reduces uric acid levels and modifies the signs associated with copper deficiency in rats fed fructose. Free Radical Biol. Med. 20: 595-600. Fine, A. M. (2000) Oligomeric proanthocyanidin complexes: history, structure, and phytopharmaceutical applications. Altern. Med. Rev. 5(2): 144-51. Finkel, T. and N. J. Holbrook (2000) Oxidants, oxidative stress and the biology of ageing. Nature 408(9) : 239-247. Flamini, G., E. Antognoli, and I. Morelli (2001) Two flavonoids and other compounds from the aerial parts of Centaurea bracteata from Italy. Phytochemistry 57: 559-564. Gyamfi, M. A., M. Yonamine, and Y. Aniya (1999) Free-radical scavenging action of medicinal herbs from Ghana Thonningia sanguinea on experimentally-induced liver injuries. General Pharmacol. 32 (6): 661-667. Halliwell, B. and J. M. C. Gutteridge (1990) Role of free radicals and catalytic metal ions in human disease: an overview. Methods Enzymol. 186: 1-85. Harwood, C. E. (1998) Human food potential of the seeds of some Australian dry-zone Acacia species. J. Arid. Environ. 27: 27-35. Hayashi, T., K. Sawa, M. Kawasaki, M. Arisawa, M. Shimizu, and N. Morita (1988) Inhibition of cow's milk xanthine oxidase by flavonoids. J. Nat. Prod. 51: 345-348. Hsiaoa, G., M. Y. Shen, W. C. Chang, Y. W. Cheng, S. L. Pan, Y. H. Kuo, T. F. Chen, and J. R. Sheu (2003) A novel antioxidant, octyl caffeate, suppression of LPS/IFN-γ-induced inducible nitric oxide synthase gene expression in rat aortic smooth muscle cells. Biochem. Pharm. 65(8): 1383-1392. Hu, M., and B. Tomlinson (2008) Febuxostat in the management of hyperuricemia and chronic gout: a review. Ther Clin Risk Manag. 4(6): 1209-1220. Ishimaru, K., Nonaka, G. and I. Nishioka (1987) Phenolic glucoside gallates from Quercus mongolica and Q. acutissima. Phytochem. 26: 1147-1152. Jacob, R. A. (1995) The integrated antioxidant system. Nutr. Res. 15: 755-766. Jiao, R. H., H. M. Ge, D. H. Shi, and R. X. Tan (2006) An apigenin derived xanthine oxidase inhibitor from Palhinhaea cernua. J. Nat. Prod. 69 (7): 1089-1091. Kalia, K., K. Sharma, H. P. Singh, and B. Singh (2008) Effects of Extraction methods on phenolic contents and antioxidant activity in aerial parts of Potentilla Atrosanguinea Lodd. and quantification of its phenolic constituents by RP-HPLC. J. Agric. Food Chem. 56(21): 10129-10134. Karawita, R., N. Siriwardhana, K. W. Lee, M. S. Heo, I. K. Yeo, Y. D. Lee, and Y. J. Jeon (2005) Reactive oxygen species scavenging, metal chelation, reducing power and lipid oxidation inhibition properties of different solvent fractions from Hizikia fusiformis. Eur. Food Res. Technol. 220: 363-371. Kazuma, K., N. Noda, and M. Suzuki (2003) Malonylated flavonol glycosides from the petals of Clitoria ternatea. Phytochemistry 62: 229-237. Kirby, A. J. and R. J. Schmidt (1997) The antioxidant activity of Chinese herbs for eczema and of placebo herbs-1. J. Ethnopharmacol. 56: 103-108. Kong, L. D., Y. Caia, W. W. Huanga, H. K. C. Christopher, and R. X. Tan (2000) Inhibition of xanthine oxidase by some Chinese medicinal plants used to treat gout. J. Ethnopharmacol.73: 199-207. Kruczynski, A., and Hill, B. T. (2001) Vinflunine, the latest Vinca alkaloid in clinical development. A review of its preclinical anticancer properties. Crit. Rev. Oncol. Hematol. 40(2): 159-173. Kujala, T. S., J. M. Loponen, K. D. Klika, and K. Pihlaja (2000) Phenolics and betacyanins in red beetroot (Beta vulgaris) root: distribution and effect of cold storage on the content of total phenolics and three individual compounds. J. Agric. Food Chem. 48: 5338-5342. Kohen, R., and A. Nyska (2002) Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol. 30(6): 620-650. Li, R. W., S. P. Myers, D. N. Leach, G. D. Lin, and G. Leach (2003) A cross-cultural study: anti-inflammatory activity of Australian and Chinese plants. J. Ethnopharmacol. 85: 25-32. Lin, C. M., C. S. Chen, C. T. Chen, Y. C. Liang, and J. K. Lin (2002) Molecular modeling of flavonoids that inhibits xanthine oxidase. Biochem. Biophys. 294: 167-172. Lin, J. K., P. C. Chen, C. T. Ho, and S. Y. Lin-Shiau (2000) Inhibition of xanthine oxidase and suppression of intracellular reactive oxygen species in HL-60 cells by theaflavin-3,3‘-digallate, (−)-epigallocatechin-3-gallate, and propyl gallate. J. Agric. Food Chem. 48(7): 2736-2743. Lippi, G., G. C. Guidi, A. Nevill, and C. Boreham (2008) The growing trend of scientific interest in sports science research. J. Sports Sciences. 26 (1): 1-2. Ljubuncic, P., S. Dakwar, I. Portnaya, U. Cogan, H. Azaizeh, and A. Bomzon (2006) Aqueous extracts of Teucrium polium possess remarkable antioxidant activity in vitro. Evidence Based Complementary Altern. Med. 3(3): 329-338. Ma, X., W. Tian, L. Wu, X. Cap, and I. Yoichiro (2005) Isolation of quercetin-3-O-L-rhamnoside from Acer truncatum Bunge by high speed counter current chromatography. J. Chromato. 1070: 211-214. Marnett, L. J. (2000) Oxyradicals and DNA damage. Carcinogenesis. 21(3) : 361-370. Naik, G. H., K. I. Priyadarsini, J. G. Satav, M. M. Banavalikar, D. P. Sohoni, M. K. Biyani, and H. Mohan (2003) Comparative antioxidant activity of individual herbal components used in Ayurvedic medicine. Phytochem. 63: 97-104. Nagao, A., M. Seki, and H. Kobayashi (1999) Inhibition of xanthine oxidase by flavonoids. Biosci. Biotechnol. Biochem. 63(10): 1787-1790. Oyaizu, M. (1986) Antioxidative activity of browning products of glucosamine fractionated by organic solvent and thin-layer chromatography. Nippon Shokuhin Kogyo Gakkaishi. 35:771-775. Packer, L., G. Rimbach, and F. Virgili (1999) Antioxidant activity and biologic properties of a procyanidin-rich extract from pine (Pinus maritima) bark, pycnogenol. Free Radic. Bio. Med. 27: 704-724. Pauff, J. M. and R. Hille (2009) Inhibition studies of bovine xanthine oxidase by luteolin, silibinin, quercetin, and curcumin. J. Nat. Prod. 72(4): 725-731. Perry, R. J., and J. R. Hodges (2000) Differentiating frontal and temporal variant frontotemporal dementia from Alzheimer’s disease. Neurology. 54: 2277-2284. Pietta, P. G. (2000) Flavonoids as antioxidants. J. Nat. Prod. 1035-1042. Popoca, J., A. Aguilar, D. Alonso, and M. L. Villarreal (1998) Cytotoxic activity of selected plants used as antitumorals in Mexican traditional medicine. J. Ethnopharmacol. 59: 173-177. Quettier-Deleu, C., G. Bernard, V. Jacques, D. Thierry, B. Claaude, L. Michel, C. Micheline, C. Jean-Claude, B. Francois, and T. Francis (2000) Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J. Ethnopharmacol. 72: 35-42. Quinn, M. T., S. Parthasarathy, L. G. Fong, and D. Steinberg (1985) Oxidatively modified low density lipoproteins: a potential role in recruitment and retention of monocyte/macrophages during atherogenesis. PNAS. 84(9): 2995-2998. Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26: 1231-1237. Reddy, A. C., and B. R. Lokesh (1994) Studies on the inhibitory effects of curcumin and eugenol on the formation of reactive oxygen species and the oxidation of ferrous iron. Mol Cell Bio. 137: 1-8. Sarawek, S., B. Feistel, I. Pischel, and V. Butterweck (2008) Flavonoids of Cynara scolymus possess potent xanthinoxidase inhibitory activity in vitro but are devoid of hypouricemic effects in rats after oral application. Planta. Med. 74(3): 221-227. Sato, M., N. Ramarathnam, Y. Suzuki, T. Ohkubo, M. Takeuchi, and H. Ochi (1996) Varietal differences in the phenolic content and superoxide radical scavenging potential of wines from different sources. J. Agric. Food Chem. 44:37-41. Seigler, D. S. (2003) Phytochemistry of Acacia-sensu lato. Biochem. Syst. Ecol. 31: 845-873. Shimoto, T., N. Ashizawa, K. Matzumoto, T. Nakazawa, and O. Nagata (2005) Simultaneous treatment with citrate prevents nephropathy induced by FYX-051, a xanthine oxidoreductase inhibitor in rats. Toxicolog. Sci. 87: 267. Shoji, A., H. Yamanaka, and N. Kamatani (2004) A retrospective study of the relationship between serum urate level and recurrent attacks of gouty arthritis: Evidence for reduction of recurrent gouty arthritis with antihyperuricemic therapy. Arthritis Rheum. 51(3): 321-325. Sule. M. I., A. K. Haruna, U. U. Pateh, .A. Ahmadu, A. Ambi, and M. S. Sallau (2005) Phytochemical investigations of leaf, fruit and root bark of Olax manni Oliv. Olacaceae. Chem. Class Journal. 2: 22-24. Sun, B., J. M. R. Silva, and I. Spranger (1998) Critical factors of vanillin assay for catechins and proanthocyanidins. J. Agric. Food Chem. 46: 4267-4274. Sun, D. W., Z. C. Zhao, L. Y. Foo, and H. B. Wong (1991) Flavonols from Myrica esculenta bark. Chem. Ind. For. Prod. 11: 251-257. Tung, Y. T., J. H. Wu, C. Y. Huang, Y. H. Kuo, and S. T. Chang (2007) Antioxidant activities of natural phenolic compounds from Acacia confusa bark. Bioresource Technol. 98: 1120-1123. Unno, T., A. Sugimoto, and T. Kakuda (2004) Xanthine oxidase inhibitors from the leaves of Lagerstroemia speciosa. Pers. J. Ethnopharmacol. 93: 391-395. Wang, S. Y., Y. H. Kuo, H. N. Chang, P. L. Kang, H. S. Tsay, K. F. Lin, N. S. Yang, and L. F. Shyur (2002) Profiling and characterization antioxidant activities in Anoectochilus formosanus Hayata. J. Agric. Food. Chem. 50: 1859-1865. Wang, S. Y., C. W. Yang, J. W. Liao, W. W. Zhen, F. H. Chu, and S. T. Chang (2008) Essential oil from leaves of Cinnamomum osmophloeum acts as a xanthine oxidase inhibitor and reduces the serum uric acid levels in oxonate-induced mice. Phytomed. 15(11): 940-945. Wolfe, K. L., X. Kang, X. He, M. Dong, Q. Zhang, and R. H. Liu (2008) Cellular antioxidant activity of common fruits. J. Agric. Food Chem. 56(18): 8418-8426. Wu, J. H., Y. T. Tung, S. Y. Wang, L. F. Shyur, Y. H. Kuo, and S. T. Chang (2005) Phenolic antioxidants from the heartwood of Acacia confusa. J. Agric. Food Chem. 53: 5917-5921. Wu, J. H., Y. T. Tung, S. C. Chien, S. Y. Wang, Y. H. Kuo, L. F. Shyur, and S. T. Chang (2008) Effect of phytocompounds from the heartwood of Acacia confusa on inflammatory mediator production. J. Agric. Food Chem. 56: 1567-1573. Yamamoto, F., H. Kasai, T. Bessho, M.-H. Chung, H. Inoue, E. Ohtsuka, T. Hori, and S. Nishimura (2005) Ubiquitous presence in mammalian cells of enzymatic activity specifically cleaving 8-hydroxyguanine-containing DNA. Cancer Sci. 83(4): 351-357. Yokozawa, T., E. Dong, Z. W. Liu, and M. Shimizu (1997) Antioxidative activity of flavones and flavonols in vitro. Phytother Res. 11: 446-449. Yun, M. R., S. I. Dong, J. L. Seung, W. W. Joong, K. W. Hong, S. S. Bae, and C. D. Kim (2008) 4-hydroxynonenal contributes to macrophage foam cell formation through increased expression of class A scavenger receptor at the level of translation. Free Radic. Biol. Med. 45(2): 177-183. Zheng, Y., X. K. Li, Y. Wang, and L. Cai (2008) The role of zinc, copper and iron in the pathogenesis of diabetes and diabetic complications: therapeutic effects by chelators. Hemoglobin 32 (1-2): 135-145. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/42760 | - |
| dc.description.abstract | Acacia confusa Merr. is an indigenous tree species that grows in Taiwan. In this study, phytochemical characteristics and bioactivities of the A. confusa twig and branch extracts were investigated. Antioxidant activities were evaluated by DPPH radical scavenging and superoxide radical scavenging assays, lipid peroxidation assay, reducing power and metal chelating ability on ferrous ions assays, and trolox equivalent antioxidant capacity (TEAC) assay. Meanwhile, total phenolic contents, total proanthocyanidin contents and total flavonoids contents were also measured. Results demonstrated that the extracts of 5 cm-branch bark possessed the best antioxidant activities and highest phenolics contents among twig and branches, indicating that branches with larger diameters have better antioxidant activities and higher phenolic contents. Furthermore, the antioxidant activities of branch extracts are stronger in the bark than in the wood.
Among all fractions, both EtOAc fractions of A. confusa twig extracts and 5 cm-branch bark extracts have the best activities. Thin layer chromatography (TLC), column chromatography (CC), and high performance liquid chromatography (HPLC) were further employed to separate active phytochemicals and then chemical structures were identified using mass spectroscopy and nucleus magnetic resonance spectroscopy (NMR). Three catechins and one flavonoid were isolated from EtOAc fraction of 5 cm-branch bark extracts, including catechin, catechin-3-O-α-rhamnopyranoside, epi-catechin, and quercetin-3-O-α- rhamnopyranoside. Five flavonoids were isolated from EtOAc fraction of twig extracts, including myricetin-3-O-β-glucopyranoside, myricetin-3-O-α-rhamnopyranoside, quercetin-3-O-α-rhamnopyranoside, myricetin -3-O-(2’’-O-galloyl)-α-rhamnopyranoside, and luteolin. Results showed the constituents are significantly different between twig and branch extracts. Catechins are the major compounds in 5 cm-branch bark extracts, while flavonoid glycosides are the majority in twig extracts. Despite both branch and twig extracts showed no significant inhibitory effects on xanthine oxidase (IC50 values are 110.0 and 113.0 μg/mL, separately), soluble fractions were also evaluated. Only EtOAc fraction of twig extracts showed better inhibitory effect with IC50 value of 26.6 μg/mL. After acid hydrolysis, the xanthine oxidase inhibitory effect was improved with decreased IC50 value of 15.5 μg/mL. The hydrolyte of EtOAc fraction also had better antioxidant activities. Major myricetin and minor quercetin were identified in the hydrolyte. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T01:22:17Z (GMT). No. of bitstreams: 1 ntu-98-R96625010-1.pdf: 1817269 bytes, checksum: 1423dc23caf45d61e3f42bfa8e1b22bb (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 目錄 I
圖目次 V 表目次 IX 摘要 XII Abstract XIV 壹、前言 1 貳、文獻回顧 3 一、活性氧與抗氧化物質 3 （一）活性氧之生成來源 3 （二）活性氧之種類 4 1. 超氧自由基 5 2. 過氧化氫 6 3. 氫氧自由基 6 4. 脂質過氧化自由基 6 （三）氧化作用之傷害 7 1. 發炎反應 8 2. 動脈硬化症 10 3. 癌症 11 （四）抗氧化防禦系統 11 1. 酵素性抗氧化防禦系統 11 2. 非酵素性抗氧化防禦系統 12 二、植物抽出物之醫療與保健用途 13 三、相思屬植物 14 （一）相思屬之研究 15 （二）本土相思樹之利用 15 （三）本土相思樹之研究 16 四、抑制黃嘌呤氧化酶活性研究 18 （一）痛風與高尿酸血症 18 （二）痛風成因及高尿酸症引發之風險 18 （三）痛風之臨床醫療 20 （四）植物抽出物之抑制黃嘌呤氧化酶活性研究 20 參、材料與方法 22 一、試驗流程 22 二、試驗材料 22 （一）相思樹枝條 22 （二）試驗藥品與溶劑 23 三、試驗方法 24 （一）相思樹枝條抽出物之萃取 24 （二）抽出物之分離與純化 24 1. 溶劑分配層析 24 2. 管柱層析 25 3. 高效能液相層析 25 （三）化合物結構鑑定 26 1. 質譜鑑定 26 2. 核磁共振圖譜鑑定 26 （四）相思樹枝條抽出物之酸水解 26 （五）相思樹枝條抽出物之抗氧化活性試驗 27 1. DPPH自由基清除試驗 27 2. 超氧自由基清除試驗 27 3. 總抗氧化能力試驗 28 4. 還原能力試驗 28 5. 抑制脂質過氧化試驗 29 6. 亞鐵離子螯合試驗 29 （六）相思樹枝條抽出物之酚類化合物含量測定 30 1. 總酚類含量測定 30 2. 原花青素含量測定 30 3. 總黃酮類含量測定 31 （七）相思樹枝條抽出物抑制黄嘌呤氧化酶之活性試驗 31 （八）統計分析 32 肆、結果與討論 33 一、相思樹不同粗細枝條抽出物之抗氧化能力及總酚類含量測定 33 二、相思樹粗枝條皮部及材部抽出物之抗氧化能力及總酚類含量測定 34 （一）粗枝條皮部及材部抽出物之抗氧化能力及總酚類含量 34 1. 清除DPPH自由基能力 34 2. 清除超氧自由基能力 35 3. 還原能力 35 （二）5 cm枝條皮部及材部抽出物之酚類化合物含量 36 1. 總酚類含量 36 2. 原花青素含量 37 3. 總黃酮含量 37 三、相思樹5 cm枝條皮部抽出物及其各可溶部之抗氧化能力 38 （一）5 cm枝條皮部抽出物及其各可溶部之收率 38 （二）5 cm枝條皮部抽出物及其各可溶部之抗氧化能力 39 1. 清除DPPH自由基能力 39 2. 清除超氧自由基能力 40 3. 總抗氧化能力 41 4. 還原能力 42 5. 抑制脂質過氧化能力 43 6. 亞鐵離子螯合能力 44 （三）5 cm枝條皮部抽出物及其各可溶部之總酚類含量測定 45 1. 總酚類含量 45 2. 原花青素含量 45 四、相思樹5 cm枝條皮部具抗氧化活性成分之分離 46 （一）乙酸乙酯次分離部之抗氧化能力 47 （二）乙酸乙酯次分離部之酚類化合物含量測定 49 1. 總酚類含量 49 2. 原花青素含量 50 3. 總黃酮含量 50 （三）5 cm枝條皮部抽出物之之抗氧化活性成分 52 五、相思樹細枝抽出物之抗氧化能力與酚類化合物含量 53 （一）細枝抽出物之抗氧化能力 53 （二）細枝抽出物之酚類化合物含量 54 （三）細枝抽出物之抗氧化活性成分 55 六、相思樹枝條抽出物之抗氧化活性成分鑑定 56 七、相思樹抽出物之抑制黄嘌呤氧化酶活性 86 （一）5 cm枝條皮部抽出物及其各可溶部之抑制黄嘌呤氧化酶活性 87 （二）細枝抽出物及其各可溶部之抑制黄嘌呤氧化酶活性 89 八、細枝乙酸乙酯可溶部酸水解後抗氧化能力及抑制黄嘌呤氧化酶活性 91 （一）乙酸乙酯可溶部與酸水解產物之抗氧化能力及抑制黄嘌呤氧化酶活性 91 （二）乙酸乙酯可溶部與酸水解產物之成分分析 93 九、化合物1-9之抗氧化能力及抑制黄嘌呤氧化酶活性 95 伍、結論 98 陸、參考文獻 99 | |
| dc.language.iso | zh-TW | |
| dc.subject | 相思樹 | zh_TW |
| dc.subject | 抗氧化活性 | zh_TW |
| dc.subject | 粗枝條 | zh_TW |
| dc.subject | 黃嘌呤氧化 | zh_TW |
| dc.subject | 兒茶素 | zh_TW |
| dc.subject | 抽出成分 | zh_TW |
| dc.subject | 黃酮醣 | zh_TW |
| dc.subject | Acacia confusa | en |
| dc.subject | xanthine oxidase | en |
| dc.subject | twig | en |
| dc.subject | flavonoid glycoside | en |
| dc.subject | extracts | en |
| dc.subject | catechin | en |
| dc.subject | branch | en |
| dc.subject | antioxidant activity | en |
| dc.title | 相思樹枝條抽出物之抗氧化活性及抑制黃嘌呤氧化酶活性 | zh_TW |
| dc.title | Antioxidant Activity and Xanthine Oxidase Inhibitory Activity of Extracts from Acacia confusa Twigs and Branches | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張惠婷(Hui-Ting Chang),蘇裕昌(Yu-Chang Su),王升陽(Sheng-Yang Wang),謝瑞忠(Jui-Chung Hsieh) | |
| dc.subject.keyword | 相思樹,抗氧化活性,粗枝條,兒茶素,抽出成分,黃酮醣,黃嘌呤氧化, | zh_TW |
| dc.subject.keyword | Acacia confusa,antioxidant activity,branch,catechin,extracts,flavonoid glycoside,twig,xanthine oxidase, | en |
| dc.relation.page | 106 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2009-07-24 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 森林環境暨資源學研究所 | zh_TW |
| 顯示於系所單位： | 森林環境暨資源學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-98-1.pdf 未授權公開取用 | 1.77 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。