3T3纖維母細胞在三維微環境中的形態分析

Wan-Jung Lin; 林宛瑢

請用此 Handle URI 來引用此文件： http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/42653

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DC 欄位	值	語言
dc.contributor.advisor	高涌泉(Yeong-Chuan Kao)
dc.contributor.author	Wan-Jung Lin	en
dc.contributor.author	林宛瑢	zh_TW
dc.date.accessioned	2021-06-15T01:18:51Z	-
dc.date.available	2013-08-20
dc.date.copyright	2011-08-20
dc.date.issued	2011
dc.date.submitted	2011-08-16
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dc.identifier.uri	http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/42653	-
dc.description.abstract	我們用大小均一、排列整齊的明膠泡泡製造細胞鷹架，並在其中養3T3纖維母細胞。我們對細胞如何對應其外微環境協調其細胞骨架感興趣。我們利用微流體通道的特性製造一系列尺寸不同孔徑但同孔隙率的鷹架，種進3T3纖維母細胞後用Phalloidin 染細胞內部的肌動蛋白，利用共軛焦顯微術取得細胞影像。用Imaris重建三維影像，針對細胞內三個軸向長度做統計．	zh_TW
dc.description.abstract	We have used mono-dispersed and self-packing gelatin bubbles for our scaffolds. 3T3 fibroblasts were used for our cell model. We were interested in how cells responded to their environment providing different geometries and mechanical properties such as surface curvature and stiffness. We utilized soft lithography to construct our scaffolds of different pore sizes and gas fractions. We cultured 3T3 in the scaffolds, stained their cytoskeletons with Phalloidin and took images with confocal microscopy. We reconstructed cell images using Imaris and took measurements of cells in scaffolds.	en
dc.description.provenance	Made available in DSpace on 2021-06-15T01:18:51Z (GMT). No. of bitstreams: 1 ntu-100-R94222026-1.pdf: 2196225 bytes, checksum: df10b7d2a92796662b1bb5b8dc24880b (MD5) Previous issue date: 2011	en
dc.description.tableofcontents	摘要.................................................................................................................................i Abstract.........................................................................................................................ii List of Figures...............................................................................................................v Chapter1. Introduction................................................................................................1 1.1 Fibroblasts and their morphology.....................................................................1 1.2 Three-Dimensional Cell Culture......................................................................3 Chapter2. Experiments................................................................................................4 2.1 Fabrication of gelatin scaffolds with mono-disperse pore size........................4 2.2 Cell Culture, Seeding, Staining, and Observation...........................................6. 2.2.1 NIH/3T3 Cells and Seeding..................................................................6 2.2.2 Staining and Imaging............................................................................6 Chapter3. Results and Discussion...............................................................................7 3.1 Cell Morphology in Different pore-size scaffolds............................................7 3.1.1 Measurements of Cells......................................................................9 3.1.2 Cell Volume Dispersions and Elongations......................................11 Chapter4. Conclusion and Outlook..........................................................................14 Reference.....................................................................................................................15 Appendix A. Montages of 3T3 Fibroblasts in Gelatin Scaffolds............................16
dc.language.iso	en
dc.subject	均一大小孔洞	zh_TW
dc.subject	三維培養	zh_TW
dc.subject	3T3纖維母細胞	zh_TW
dc.subject	形態學	zh_TW
dc.subject	明膠培養鷹架	zh_TW
dc.subject	gelatin scaffold	en
dc.subject	3D culture	en
dc.subject	monodisperse pores	en
dc.subject	3T3 fibroblasts	en
dc.subject	morphology	en
dc.title	3T3纖維母細胞在三維微環境中的形態分析	zh_TW
dc.title	Analyzing the Morphology of 3T3 fibroblasts in Three Dimensional Microenvironment	en
dc.type	Thesis
dc.date.schoolyear	99-2
dc.description.degree	碩士
dc.contributor.oralexamcommittee	林耿慧(Keng-Hui Lin),黃仲仁(Jung-Ren Huang)
dc.subject.keyword	三維培養,3T3纖維母細胞,形態學,明膠培養鷹架,均一大小孔洞,	zh_TW
dc.subject.keyword	3D culture,3T3 fibroblasts,morphology,gelatin scaffold,monodisperse pores,	en
dc.relation.page	24
dc.rights.note	有償授權
dc.date.accepted	2011-08-16
dc.contributor.author-college	理學院	zh_TW
dc.contributor.author-dept	物理研究所	zh_TW
顯示於系所單位：	物理學系

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