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Gene expression profiles of growth hormone, gonadotropins and proopiomelanocortin in yellow stage of wild female Japanese eel, Anguilla japonica
|Authors:||Sheryll Grospe Hipolito|
Japanese eel,growth hormone,follicle stimulating hormone,luteinizing hormone,glycoprotein,proopiomelanocortin,
|Publication Year :||2009|
|Abstract:||日本鰻 (Anguilla japonica) 為降海洄游魚類，棲息於日本、台灣、中國大陸及韓國。由於它一生橫跨淡水及海水水域且歷經多次變態，此特殊的生活史，使日本鰻非常適合做為硬骨魚類內分泌調控系統之研究。
實驗樣本由2006年9月至2008年3月採集於台灣高屏溪下游之野生鰻，測量魚體全長、體重、並計算形態與生理指數：眼徑指數 (OI)、胸鰭指數 (FI)、肝指數 (HSI) 及生殖腺指數 (GSI)。自一個或數個腦下垂體萃取出total RNA並以Real-time-qPCR分析腦下垂體賀爾蒙之表現量。魚體全長和OI 呈顯著的正相關；FI呈負相關；但與HIS 及GSI 無關連性。另一方面，魚體全長和 GH、FSHβ、LHβ、GPα、POMC 表現量則無相關性。GSI和GH、FSHβ、LHβ呈顯著的正相關。在相關性分析中GH、FSHβ、LHβ及POMC的表現量兩兩呈現顯著正相關，但GPα表現量則和上述荷爾蒙無相關。
Anguilla japonica, known as the Japanese eel, is a catadromous fish that can be found in Japan, Taiwan, China and Korea. Owing to its unique life cycle, spending parts of its life in freshwater and seawater environment where they undergo multiple morphophysiological changes made it a suitable species for investigating endocrine regulatory system of teleost fish.
Eel pituitary gland contains a variety of secretory cells which through the actions of hypothalamus target specific cell types that triggers hormonal secretions including growth hormone, reproductive hormones, proopiomelanocortin, thyrotrophin and prolactin that are important for growth, development, metabolism, reproductive and nervous system functions. Although considerable advances have been made in understanding the roles of hormones in the context of osmoregulation, reproduction, growth and differentiation and energy, some information concerning these processes and their possible interactions with each other are still lacking and needs further studies. The main objective of this study is to evaluate the potential interaction between growth hormone (GH), follicle stimulating hormone β-subunit (FSHβ), luteinizing hormone β-subunit (LHβ), glycoprotein hormone α-subunit (GPα) and proopiomelanocortin (POMC) at the pituitary level in wild caught female yellow stage Japanese eels.
Wild Japanese eels were obtained from the Kaoping River of Taiwan between September 2006 and March 2008. Total length and body weights were measured and morphometric indices including ocular index (OI), fin index (FI), hepatosomatic index (HSI) and gonadosomatic index (GSI) were calculated. Total RNA was extracted from individual or pooled pituitary glands and the expression levels of pituitary hormones were determined by Real-Time-qPCR. Total length showed a significant and positive correlation with OI and a negative correlation with FI while no correlations were observed with HSI and GSI. On the other hand, total length revealed no correlations with GH, FSHβ, LHβ, GPα and POMC. The results showed a significant and positive correlation of GH, FSHβ and LHβ to GSI. Correlation analysis between pituitary hormones revealed significant and positive correlations among GH, FSHβ, LHβ and POMC. However, no correlations were observed between these hormones and GPα.
The results demonstrated that, at the pituitary level of yellow stage of wild female Japanese eels, hormone interaction occurs on growth hormone, reproductive hormones and proopiomelanocortin. Their interactions may be affected by downstream signaling factors, by anatomical distribution of the secretory cells resulting in paracrine communication or by dual actions of hypothalamic neuroendocrine hormones.
|Appears in Collections:||漁業科學研究所|
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