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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 閔明源(Ming-Yuan Min) | |
dc.contributor.author | Yeechan Wu | en |
dc.contributor.author | 吳怡賢 | zh_TW |
dc.date.accessioned | 2021-06-15T01:12:17Z | - |
dc.date.available | 2011-08-19 | |
dc.date.copyright | 2009-08-19 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-07-30 | |
dc.identifier.citation | References
Aragon C, Lopez-Corcuera B (2003) Structure, function and regulation of glycine neurotransporters. Eur J Pharmacol 479:249-262. Bajic D, Proudfit HK (1999) Projections of neurons in the periaqueductal gray to pontine and medullary catecholamine cell groups involved in the modulation of nociception. J Comp Neurol 405:359-379. Bajic D, Van Bockstaele EJ, Proudfit HK (2001) Ultrastructural analysis of ventrolateral periaqueductal gray projections to the A7 catecholamine cell group. Neuroscience 104:181-197. Bear MF, Connors BW, Paradiso MA (2007) Neuroscience : exploring the brain, 3rd Edition. Philadelphia, PA: Lippincott Williams & Wilkins. Bettler B, Tiao JY (2006) Molecular diversity, trafficking and subcellular localization of GABAB receptors. Pharmacol Ther 110:533-543. Bettler B, Kaupmann K, Mosbacher J, Gassmann M (2004) Molecular structure and physiological functions of GABA(B) receptors. Physiol Rev 84:835-867. Billinton A, Upton N, Bowery NG (1999) GABA(B) receptor isoforms GBR1a and GBR1b, appear to be associated with pre- and post-synaptic elements respectively in rat and human cerebellum. Br J Pharmacol 126:1387-1392. Bischoff S, Leonhard S, Reymann N, Schuler V, Shigemoto R, Kaupmann K, Bettler B (1999) Spatial distribution of GABA(B)R1 receptor mRNA and binding sites in the rat brain. J Comp Neurol 412:1-16. Bouvier M (2001) Oligomerization of G-protein-coupled transmitter receptors. Nat Rev Neurosci 2:274-286. Bowery NG, Hudson AL, Price GW (1987) GABAA and GABAB receptor site distribution in the rat central nervous system. Neuroscience 20:365-383. Bowery NG, Bettler B, Froestl W, Gallagher JP, Marshall F, Raiteri M, Bonner TI, Enna SJ (2002) International Union of Pharmacology. XXXIII. Mammalian gamma-aminobutyric acid(B) receptors: structure and function. Pharmacol Rev 54:247-264. Calver AR, Davies CH, Pangalos M (2002) GABA(B) receptors: from monogamy to promiscuity. Neurosignals 11:299-314. Cedarbaum JM, Aghajanian GK (1976) Noradrenergic neurons of the locus coeruleus: inhibition by epinephrine and activation by the alpha-antagonist piperoxane. Brain Res 112:413-419. Clark FM, Proudfit HK (1991a) The projection of noradrenergic neurons in the A7 catecholamine cell group to the spinal cord in the rat demonstrated by anterograde tracing combined with immunocytochemistry. Brain Res 547:279-288. Clark FM, Proudfit HK (1991b) The projection of locus coeruleus neurons to the spinal cord in the rat determined by anterograde tracing combined with immunocytochemistry. Brain Res 538:231-245. Clark FM, Proudfit HK (1991c) Projections of neurons in the ventromedial medulla to pontine catecholamine cell groups involved in the modulation of nociception. Brain Res 540:105-115. Clark FM, Proudfit HK (1993) The projections of noradrenergic neurons in the A5 catecholamine cell group to the spinal cord in the rat: anatomical evidence that A5 neurons modulate nociception. Brain Res 616:200-210. Couve A, Filippov AK, Connolly CN, Bettler B, Brown DA, Moss SJ (1998) Intracellular retention of recombinant GABAB receptors. J Biol Chem 273:26361-26367. Dennis SG, Melzack R, Gutman S, Boucher F (1980) Pain modulation by adrenergic agents and morphine as measured by three pain tests. Life Sci 26:1247-1259. Drake CT, Bausch SB, Milner TA, Chavkin C (1997) GIRK1 immunoreactivity is present predominantly in dendrites, dendritic spines, and somata in the CA1 region of the hippocampus. Proc Natl Acad Sci U S A 94:1007-1012. Gaspary HL, Wang W, Richerson GB (1998) Carrier-mediated GABA release activates GABA receptors on hippocampal neurons. J Neurophysiol 80:270-281. Hill DR (1985) GABAB receptor modulation of adenylate cyclase activity in rat brain slices. Br J Pharmacol 84:249-257. Hill DR, Bowery NG (1981) 3H-baclofen and 3H-GABA bind to bicuculline-insensitive GABA B sites in rat brain. Nature 290:149-152. Holden JE, Proudfit HK (1998) Enkephalin neurons that project to the A7 catecholamine cell group are located in nuclei that modulate nociception: ventromedial medulla. Neuroscience 83:929-947. Holden JE, Pizzi JA (2003) The challenge of chronic pain. Adv Drug Deliv Rev 55:935-948. Holden JE, Van Poppel AY, Thomas S (2002) Antinociception from lateral hypothalamic stimulation may be mediated by NK(1) receptors in the A7 catecholamine cell group in rat. Brain Res 953:195-204. Hunt SP, Mantyh PW (2001) The molecular dynamics of pain control. Nat Rev Neurosci 2:83-91. Isaacson JS, Solis JM, Nicoll RA (1993) Local and diffuse synaptic actions of GABA in the hippocampus. Neuron 10:165-175. Jensen K, Chiu CS, Sokolova I, Lester HA, Mody I (2003) GABA transporter-1 (GAT1)-deficient mice: differential tonic activation of GABAA versus GABAB receptors in the hippocampus. J Neurophysiol 90:2690-2701. Jonas P, Bischofberger J, Sandkuhler J (1998) Corelease of two fast neurotransmitters at a central synapse. Science 281:419-424. Kaupmann K, Huggel K, Heid J, Flor PJ, Bischoff S, Mickel SJ, McMaster G, Angst C, Bittiger H, Froestl W, Bettler B (1997) Expression cloning of GABA(B) receptors uncovers similarity to metabotropic glutamate receptors. Nature 386:239-246. Kaupmann K, Malitschek B, Schuler V, Heid J, Froestl W, Beck P, Mosbacher J, Bischoff S, Kulik A, Shigemoto R, Karschin A, Bettler B (1998) GABA(B)-receptor subtypes assemble into functional heteromeric complexes. Nature 396:683-687. Kulik A, Vida I, Lujan R, Haas CA, Lopez-Bendito G, Shigemoto R, Frotscher M (2003) Subcellular localization of metabotropic GABA(B) receptor subunits GABA(B1a/b) and GABA(B2) in the rat hippocampus. J Neurosci 23:11026-11035. Kwiat GC, Basbaum AI (1992) The origin of brainstem noradrenergic and serotonergic projections to the spinal cord dorsal horn in the rat. Somatosens Mot Res 9:157-173. Lei S, McBain CJ (2003) GABA B receptor modulation of excitatory and inhibitory synaptic transmission onto rat CA3 hippocampal interneurons. J Physiol 546:439-453. Lerma J, Herranz AS, Herreras O, Abraira V, Martin del Rio R (1986) In vivo determination of extracellular concentration of amino acids in the rat hippocampus. A method based on brain dialysis and computerized analysis. Brain Res 384:145-155. Liu QY, Schaffner AE, Chang YH, Maric D, Barker JL (2000) Persistent activation of GABA(A) receptor/Cl(-) channels by astrocyte-derived GABA in cultured embryonic rat hippocampal neurons. J Neurophysiol 84:1392-1403. Luscher C, Jan LY, Stoffel M, Malenka RC, Nicoll RA (1997) G protein-coupled inwardly rectifying K+ channels (GIRKs) mediate postsynaptic but not presynaptic transmitter actions in hippocampal neurons. Neuron 19:687-695. Margeta-Mitrovic M, Jan YN, Jan LY (2000) A trafficking checkpoint controls GABA(B) receptor heterodimerization. Neuron 27:97-106. Miguelez C, Fernandez-Aedo I, Torrecilla M, Grandoso L, Ugedo L (2009) alpha(2)-Adrenoceptors mediate the acute inhibitory effect of fluoxetine on locus coeruleus noradrenergic neurons. Neuropharmacology 56:1068-1073. Millan MJ (1999) The induction of pain: an integrative review. Prog Neurobiol 57:1-164. Millan MJ (2002) Descending control of pain. Prog Neurobiol 66:355-474. Min MY, Wu YW, Shih PY, Lu HW, Lin CC, Wu Y, Li MJ, Yang HW (2008) Physiological and morphological properties of, and effect of substance P on, neurons in the A7 catecholamine cell group in rats. Neuroscience 153:1020-1033. Mintz IM, Bean BP (1993) GABAB receptor inhibition of P-type Ca2+ channels in central neurons. Neuron 10:889-898. Moore RY, Bloom FE (1979) Central catecholamine neuron systems: anatomy and physiology of the norepinephrine and epinephrine systems. Annu Rev Neurosci 2:113-168. Neher E (1992) Correction for liquid junction potentials in patch clamp experiments. Methods Enzymol 207:123-131. Nuseir K, Proudfit HK (2000) Bidirectional modulation of nociception by GABA neurons in the dorsolateral pontine tegmentum that tonically inhibit spinally projecting noradrenergic A7 neurons. Neuroscience 96:773-783. Nuseir K, Heidenreich BA, Proudfit HK (1999) The antinociception produced by microinjection of a cholinergic agonist in the ventromedial medulla is mediated by noradrenergic neurons in the A7 catecholamine cell group. Brain Res 822:1-7. Paxinos G, Watson C (2007) The rat brain in stereotaxic coordinates, 6th Edition. Amsterdam: Elsevier. Pertovaara A (2006) Noradrenergic pain modulation. Prog Neurobiol 80:53-83. Poncer JC, McKinney RA, Gahwiler BH, Thompson SM (1997) Either N- or P-type calcium channels mediate GABA release at distinct hippocampal inhibitory synapses. Neuron 18:463-472. Prince DA, Stevens CF (1992) Adenosine decreases neurotransmitter release at central synapses. Proc Natl Acad Sci U S A 89:8586-8590. Princivalle A, Spreafico R, Bowery N, De Curtis M (2000) Layer-specific immunocytochemical localization of GABA(B)R1a and GABA(B)R1b receptors in the rat piriform cortex. Eur J Neurosci 12:1516-1520. Proudfit HK (1988) Pharmacologic evidence for the modulation of nociception by noradrenergic neurons. Prog Brain Res 77:357-370. Proudfit HK, Clark FM (1991) The projections of locus coeruleus neurons to the spinal cord. Prog Brain Res 88:123-141. Raiteri M (2008) Presynaptic metabotropic glutamate and GABAB receptors. Handb Exp Pharmacol:373-407. Reddy SV, Yaksh TL (1980) Spinal noradrenergic terminal system mediates antinociception. Brain Res 189:391-401. Rossi DJ, Hamann M (1998) Spillover-mediated transmission at inhibitory synapses promoted by high affinity alpha6 subunit GABA(A) receptors and glomerular geometry. Neuron 20:783-795. Russier M, Kopysova IL, Ankri N, Ferrand N, Debanne D (2002) GABA and glycine co-release optimizes functional inhibition in rat brainstem motoneurons in vitro. J Physiol 541:123-137. Shefner SA, Osmanovic SS (1991) GABAA and GABAB receptors and the ionic mechanisms mediating their effects on locus coeruleus neurons. Prog Brain Res 88:187-195. Stuart GJ, Dodt HU, Sakmann B (1993) Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy. Pflugers Arch 423:511-518. Suzuki R, Dickenson A (2005) Spinal and supraspinal contributions to central sensitization in peripheral neuropathy. Neurosignals 14:175-181. Thompson SM, Capogna M, Scanziani M (1993) Presynaptic inhibition in the hippocampus. Trends Neurosci 16:222-227. Westlund KN, Coulter JD (1980) Descending projections of the locus coeruleus and subcoeruleus/medial parabrachial nuclei in monkey: axonal transport studies and dopamine-beta-hydroxylase immunocytochemistry. Brain Res 2:235-264. White JH, Wise A, Main MJ, Green A, Fraser NJ, Disney GH, Barnes AA, Emson P, Foord SM, Marshall FH (1998) Heterodimerization is required for the formation of a functional GABA(B) receptor. Nature 396:679-682. Yeomans DC, Proudfit HK (1990) Projections of substance P-immunoreactive neurons located in the ventromedial medulla to the A7 noradrenergic nucleus of the rat demonstrated using retrograde tracing combined with immunocytochemistry. Brain Res 532:329-332. Yeomans DC, Proudfit HK (1992) Antinociception induced by microinjection of substance P into the A7 catecholamine cell group in the rat. Neuroscience 49:681-691. Yeomans DC, Clark FM, Paice JA, Proudfit HK (1992) Antinociception induced by electrical stimulation of spinally projecting noradrenergic neurons in the A7 catecholamine cell group of the rat. Pain 48:449-461. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/42334 | - |
dc.description.abstract | 大鼠橋腦的A7核區由正腎上腺素神經元所構成,投射至脊髓背角,一般認為跟痛覺的調控有關。然而這些A7正腎上腺素神經元的自發性活動在一般生理情況下並不高,過去研究者猜測A7正腎上腺素神經元可能受到週遭GABAergic中間神經元的持續性抑制。另外,A7核區也受到其他核區的投射,例如週邊導水管灰質區(periaquiductal gray, PAG);以及腹內側延腦(ventromedial mudlla, RVM)。這些投射中有一部分為抑制性的神經傳導物質,例如腦啡肽(enkephalin)。當來自PAG及RVM的抑制性投射抑制了A7核區的中間神經元後,正腎上腺素神經元受到的持續性抑制就被減弱,造成正腎上腺素神經元活性上升,而達到止痛的效果。本篇研究試圖證實是否A7正腎上腺素神經元在一般生理情況下,確實受到來自GABAergic中間神經元GABAB受器媒介的持續性抑制。我們在生理溫度下,以全細胞實驗方式記錄出生7-9天新生大鼠的A7核區神經元,並在實驗過程後進行免疫組織螢光染色,確認細胞為正腎上腺素神經元。我們證明GABAB受器不僅存在於正腎上腺素神經元上,也存在於投射至正腎上腺素的神經末梢。我們從三個面向來探討GABAB受器在調控正腎上腺素神經元所扮演的角色:意即對突觸後正腎上腺素神經元的影響,以及突觸前興奮性或抑制性訊息傳導的影響。並發現GABAB受器能夠降低正腎上腺素神經元的自發性放電活動,也能夠減弱投射至正腎上腺素神經元的神經訊息傳導。然而,並非所有的GABAB受器都能夠對正腎上腺素神經元產生持續性抑制,在本篇中,我們的結果顯示只有在正腎上腺素神經元上GABAB受器,才能造成顯著的持續性抑制。 | zh_TW |
dc.description.abstract | The A7 catecholamine cell group consists of noradrenergic (NAergic) neurons that project axonal terminals to the dorsal spinal cord, and are believed to play important role in modulating nociceptic input. It has been suggested that NAergic neurons receive tonic inhibition from local GABAergic interneurons which is controlled by enkephalin released from neurons in the periaquiductal gray (PAG) and ventraomedial medulla (RVM). Activation of these nuclei would relieve the tonic inhibition of A7 NAergic neurons, which in turn, would increase the release of NE in the dorsal spinal cord and result in modulation of pain. In this study, we provided electrophysiological evidences of the existence tonic inhibition mediated by GABAB receptors on A7 NAergic neurons. Whole-cell patch recording were made from A7 NAergic neurons, confirmed by post hoc immunohistochemistry using antibody against dopamine-β-hydroxylase, in sagittal brainstem slices taken from rats aged 7-9 days. We investigated the role of GABAB receptors in three dimensions: intrinsic properties of NAergic neurons; excitatory synaptc inputs to NAergic neurons; and inhibitory synaptic inputs to NAergic neurons. The present data suggested that GABAB receptors are located not only on the postsynaptic NAergic neurons, but also on the presynaptic excitatory and inhibitory axon terminals projecting to them. And these GABAB receptors could either directly reduced the spontaneous firing frequency of NAergic neurons or decreased the synaptic transmission (excitatory or inhibitory). However, not all of these GABAB receptors are tonically activated, and our data proposed that only the postsynaptic GABAB receptors mediated tonic inhibition on A7 NAergic neurons. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T01:12:17Z (GMT). No. of bitstreams: 1 ntu-98-R96b41009-1.pdf: 3760212 bytes, checksum: e8243240bf48c5bba57bd8f83c8b1c45 (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | Contents
誌謝 i 英文摘要 iii 中文摘要 v Introduction 1 The ascending and descending pathway of pain 1 The role of A7 catecholamine cell group in descending modulation of pain 2 GABAB receptors and cellular mechanism 4 Aim of this study 6 Material and Methods 8 Preparation of brain stem slices 8 Electrophysiology 8 Mesurements of liquid Junction potentials 12 Data analysis 12 Drugs 13 Filling recorded neurons with biocytin and immunocytochemistry 13 Immunohistochemistry of GABAB receptor 14 Results 16 Identification of A7 NAergic neurons in rat brainstem slices 16 A7 NAergic neurons highly expressed GABAB receptors 16 The activation of GABAB receptors decreased spontaneous firing rate of A7 NAergic neurons 17 Locally evoked excitatory postsynaptic current on A7 NAergic neurons was composed of AMPA receptors and NMDA receptors 18 The activation of GABAB receptors decreased the locally evoked excitatory transmission in A7 NAergic neurons 20 There was no GABAB receptor-mediated tonic inhibition on eEPSC 21 Even High frequency stimulation could not induce activation of GABAB receptors on excitatory axon terminals 22 Locally evoked inhibitory postsynaptic current on A7 NAergic neurons was composed of GABAA receptors and glycine receptors 23 Activation of GABAB receptors reduced the eIPSC on NAergic neurons 24 GABAB receptors might play a role on autoregulation of eIPSC 25 Discussion 27 GABAB receptor- mediated tonic inhibition on A7 NAergic spontaneous firing rate 28 GABAB receptors on excitatory axon terminals projecting to A7 NAergic neurons 29 GABAB receptors on inhibitory axon terminals projecting to A7 NAergic neurons 31 Future works 32 References 33 Figures 40 | |
dc.language.iso | en | |
dc.title | 大鼠A7核區正腎上腺素神經元GABAB受器媒介之持續性抑制 | zh_TW |
dc.title | GABAB Receptor Mediated Tonic Inhibition in Noradrenergic Neurons in Rat A7 Nucleus | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 楊琇雯(Hsiu-Wen Yang),傅毓秀(Yu-Show Fu) | |
dc.subject.keyword | 腦幹,A7正腎上腺素神經元,GABAB受器,全細胞記錄實驗,免疫組織, | zh_TW |
dc.subject.keyword | brainstem,A7 NAergic neurons,GABAB receptors,whole-cell recording,immunohistochemistry, | en |
dc.relation.page | 50 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2009-07-30 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
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