Benzo[a]pyrene、Phenanthrene、Chrysene誘導人類支氣管上皮細胞BEAS-2B Cell表現interleukin-6與 interleukin-8

Abstract

本研究選擇具代表性的多環芳香烴化合物benzo[a]pyrene、phenanthrene、chrysene觀察人類支氣管上皮細胞（BEAS-2B cell），暴露該等多環芳香烴化合物後釋放激素interleukin-6（IL-6）及interleukin-8（IL-8）的效應。PAH暴露濃度設定為低（4.22~5.54 nM）、中（98.5~138.5 nM）、高（492.5~692.5 nM）三種範圍，暴露時間為8、12、16、24、48小時，IL-6及IL-8分析使用ELISA技術。結果發現，PAH的物種、濃度與暴露時間為影響細胞分泌IL-6與IL-8的重要變項。經暴露濃度歸一化調整後的IL-6、IL-8濃度，其單位時間的變化率隨PAH之物種而異，以benzo[a]pyrene較大。劑量-效應關係似乎呈現兩個階段，且因PAH物種而有差異：暴露benzo[a]pyrene，低劑量時IL-6、IL-8效應呈線性下降，相對較高劑量時反應隨劑量的平方而增加；而暴露phenanthrene或chrysene時，IL-6、IL-8的效應先呈線性的上升而後隨劑量的平方緩降。認為PAH造成細胞分泌細胞激素，產生發炎反應的影響可能存在，在濃度與暴露時間條件接近的情況下，不排除物種是關鍵因素。

This study aimed at evaluating the induction of interleukin-6（IL-6）and interleukin-8（IL-8）while the human bronchial epithelial cells, BEAS-2B cell, exposed to authentic benzo[a]pyrene, phenanthrene, and chrysene with concentration at low（4.22~5.54 nM）, medium（98.5~138.5 nM）, and high（492.5~692.5 nM）levels. The exposure lasted for 8, 12, 16, 24, and 48 hours, before the ELISA test were applied for detecting IL-6 and IL-8. The results demonstrated that the induction of IL-6 and IL-8 varied with species of PAH, concentration and exposure time. The change rate of cytokine level per hour exposure（pg/mL-nM/hr）varied with chemical species as the levels of IL-6 and IL-8 were normalized to exposure concentration. The change rate for benzo[a]pyrene was upmost. The does-effect relationship was interpreted by using linear-quadratic model assuming that the level of IL-6 or IL-8 is related to two phases of action. For benzo[a]pyrene, the level of IL-6 and IL-8 were negatively proportional to dose at the lower dose exposure and the level of IL-6 and IL-8 were positively proportional to the square of dose at the higher dose exposure. For exposure to phenanthrene or chrysene, the levels of IL-6 and IL-8 positively increase with the increment of dose at lower dose exposure and then slightly decrease with the square of dose at higher dose exposure. The induction of proinflammatory cytokines in epithelial cell likely occurs due to exposure to polynuclear aromatic hydrocarbons, and the effects mainly depend on chemical species as the other exposure modifiers such as exposure concentration and exposure time are identical.