請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/41471
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 齊肖琪 | |
dc.contributor.author | Yi-Chun Fang | en |
dc.contributor.author | 方宜鈞 | zh_TW |
dc.date.accessioned | 2021-06-15T00:20:08Z | - |
dc.date.available | 2012-02-18 | |
dc.date.copyright | 2009-02-18 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-02-10 | |
dc.identifier.citation | 涂堅 (2004) 海鱺發光菌不活化疫苗之研發。行政院農委會家畜衛衛生試驗所。學術研討會摘要 水產動物篇。
涂堅 (2005) 發光菌(巴斯德桿菌)疫苗。行政院農委會家畜衛衛生試驗所發布。 劉秉忠、黃之暘、李國誥 (2006) 優質海鱺之種魚培育與魚苗生產。中華民國水產種苗協會優質種苗與水產養殖專刊XII, 15-30頁。 劉秉忠、蔡俊男、李國誥 (2005) 箱網養殖海鱺細菌性疫苗開發及有效性研究。漁業署養殖特刊9 魚病研究專輯22, 41-50頁。 林積陽 (2002) 養殖海鱺細菌性病原之分離及巴斯德桿菌之致病性研究。國立台灣海洋大學水產養殖學系碩士論文。 隆雪明、劉湘新、文利新 (2007) 黃耆多糖及其應用。中獸醫醫藥雜誌2期, 24-27頁。 蓋玉軒 (2005) 石斑魚神經壞死症病毒去活化疫苗的製備及效能評估。國立台灣大學動物學研究所碩士論文。 高海、李秀嵐 (2003) 中草藥多醣免疫調節劑研究進展。動物醫學進展第五期, 56頁。 古鎮鈞、陳秀男 (2000) 台灣海鱺繁、養殖技術現況與評析。海鱺箱網養殖研討會, 39-49頁。 胡梅、崔保安、張紅英、夏平安、王學彬、王遠閣、趙現敏 (2006) 板藍根、黃耆等中藥活性提取物對豬繁殖與呼吸綜合徵病毒體外作用的研究。中獸醫醫藥雜誌6期, 14-17頁。 趙智華、左福元 (2006) 中草藥添加劑的研究進展。飼料研究1期, 49-52頁。 周春風、張志亭 (2007) 中草藥飼料添加計研究概況。飼料研究2期, 24-25頁。 陳炳傑 (2003) 養殖海鱺巴斯德桿菌症疫苗之應用研究。國立台灣海洋大學水產養殖學系碩士論文。 邵廣昭、陳正平、沈士傑 (1992) 墾丁國家公園海域魚類圖鑑。內政部營建署墾丁國家公園管理處。 蔡俊男 (2005) 細菌性疫苗應用於養殖海鱺之研究。國立台灣海洋大學水產養殖學系碩士論文。 楊惠郎 (2003) 疫苗發展技術與實驗。教育部出版, 146-149頁。 王俊輝、孫志良 (2006) 黃耆的有效成分及在畜牧生產中的應用。飼料研究12期, 19-21頁。 王俊傑 (2002) 海鱺rag-1與Ig基因之選殖及其在發育過程之表現。國立成功大學生物科技研究所碩士論文。 Afonso, A., Gomes, S., Da Silva, J., Marques, F., and Henrique, M. (2005) Side effects in sea bass (Dicentrarchus labrax L.) due to intraperitoneal vaccination against vibriosis and pasteurellosis. Fish and Shellfish Immunology 19, 1-16. Aguilar, J.C., and Rodriguez, E.G. (2007) Vaccine adjuvants revisited. Vaccine 25, 3752-3762. Amend, D.F., and Fender, D.C. (1976) Uptake of bovine serum albumin by rainbow trout from hyperosmotic solutions: a model for vaccinating fish. Science. 192, 793-794. Ando, I., Tsukumo,Y., Wakabayashi, T., Akashi, S., Miyake, K., Kataoka, T., Nagai, K. (2002) Safflower polysaccharides activate the transcription factor NF-kappa B via Toll-like receptor 4 and induce cytokine production by macrophages. International immunophamacology, 2, 1155-1162. Antipa, R., Gould, G. and Amend. D.E. (1980) Vibrio anguillarum vaccination of sockeve salmon. Oncorhvnchus nerka (Walbaum). bv direct and hvoerosmoc immersion. J. Widl. Dis. 3, 161.165. Ardo, L., Yin, G., Xu, P., Varadi, L., Szigeti, G., Jeney, Z., and Jeney, G. (2008) Chinese herbs (Astragalus membranaceus and Lonicera japonica) and boron enhance the non-specific immune response of Nile tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila. Aquaculture 275, 26-33. Arijo, S., Rico, R., Chabrillon, M., Diaz-Rosales, P., Martinez-Manzanares, E., Balebona, M.C., Magariños, B., Toranzo, A.E., and Moriñigo, M.A. (2005) Effectiveness of a divalent vaccine for sole, Solea senegalensis (Kaup), against Vibrio harveyi and Photobacterium damselae subsp. piscicida. Journal of Fish Diseases 28, 33-38. Austin, B.and Austin, D.A., (1993) Bacterial Fish Pathogens: Disease in Farmed and Wild Fish, 2nd end. Ellis Horwood Limited, Chichester, pp. 359-373. Austin, B. and Austin, D.A. (1999) Bacterial fish pathogens: Diseases in farmed and wild fish. Springer/Praxis Publishing, Chichester. Austin, B., Stuckey, L. F., Robertson, P. A.W., Effendi, I. and Griffith, D. R. W. (1995) A probiotic strain of Vibrio alginolyticus effective in reducing disease caused by Aeromonas salmonicida, V. anguillarum and V. ordalii. J. Fish Dis., 18, 93-96. Bakopoulos, V., Volpatti, D., Adams, A., Galeotti, M., and Richards, R. (1997) Qualitative differences in the immune response of rabbit, mouse and sea bass, Dicentrarchus labrax, L. to Photobacterium damsela subsp. piscicida, the causative agent of fish Pasteurellosis. Fish & Shellfish Immunology 7, 161-174. Bakopoulos, V., Volpatti, D., Gusmani, L., Galeotti, M., Adams, A., and Dimitriadis, G.J. (2003) Vaccination trials of sea bass, Dicentrarchus labrax (L.), against Photobacterium damsela subsp. piscicida, using novel vaccine mixtures. Journal of Fish Diseases 26, 77-90. Bonet, R., Magarinos, B., Romalde, J.L., Simon-Pujol, M.D., Toranzo, A.E., and Congregado, F. (1994) Capsular polysaccharide expressed by Pasteurella piscicida grown in vitro. FEMS Microbiology Letters 124, 285-289. Bricknell, I., and Dalmo, R.A. (2005) The use of immunostimulants in fish larval aquaculture. Fish & Shellfish Immunology 19, 457-472. Buchmann, K., Dalsgaard, I., Nielsen, M. E., Pedersen, K., Uldal, A., Garcia, J. A., Larsen J. L. (1997) Vaccination improves survival of Baltic salmon (Salmo salar) smolts in delayed release sea ranching (net-pen period). Aquaculture 156, 335-348 Caipang, C.M.A., Hynes, N., Puangkaew, J., Brinchmann, M.F., and Kiron, V. (2008) Intraperitoneal vaccination of Atlantic cod, Gadus morhua with heat-killed Listonella anguillarum enhances serum antibacterial activity and expression of immune response genes. Fish & Shellfish Immunology 24, 314-322. Dos Santos, N.M.S., Taverne-Thiele, J.J., Barnes, A.C., Ellis, A.E., and J.H.W, M.R. (2001a) Kinetics of juvenile sea bass (Dicentrarchus labrax, L.) systemic and mucosal antibody secreting cell response to different antigens (Photobacterium damselae spp. piscicida, Vibrio anguillarum and DNP). Fish & Shellfish Immunology 11, 317-331. Dos Santos, N.M.S., Taverne-Thiele, J.J., Barnes, A.C., Van Muiswinkel, W.B., Ellis, A.E., and Rombout, J.H.W.M. (2001b). The gill is a major organ for antibody secreting cell production following direct immersion of sea bass (Dicentrarchus labrax, L.) in a Photobacterium damselae ssp. piscicida bacterin: An ontogenetic study. Fish and Shellfish Immunology 11, 65-74. Do Vale A., Manuel T. Silva, Nuno M. S. dos Santos, Diana S. Nascimento, Pedro Reis-Rodrigues, Carolina Costa-Ramos, Anthony E. Ellis and Jorge E. Azevedo (2005) AIP56, a novel plasmid-encoded virulence factor of Photobacterium damselae subsp. piscicida with apoptogenic activity against sea bass macrophages and neutrophils. Molecular Microbiology, 58, 1025-1038. Do Vale A., C.C.-R., Alexandra Silva, Daniela S. P. Silva, Fatima Gartner, Nuno M. S. dos Santos, Manuel T. Silva (2007) Systemic macrophage and neutrophil destruction by secondary necrosis induced by a bacterial exotoxin in a Gram-negative septicaemia. Cellular Microbiology 9, 988-1003. Egidius, E.C. and Anderson K. (1979) Bath immunization - a practical and nonstressing method of vaccinating sea fanned rainbow trout, Salmogairdneri Richardson, against vibriosis. J. Fish Dis. 2, 405-410. Egusa, S. (1983) Disease problems in cultured yellowtail, Seriola quinqueradiata, culture: a review. Rapports et proces-verbaux des reunions Conseil International pour l'exploration de la mer 182, 10-18. Elkamel, A.A., and Thune, R.L. (2003) Invasion and replication of Photobacterium damselae subsp. piscicida in fish cell lines. Journal of Aquatic Animal Health 15, 167-174. Ellis, A.E. (1988) Fish vaccination. Academic Press London, pp38-48. Frerichs, G.N. and Roberts, R.J. (1989) The Bacteriology of Teleosts. In: R.J. Roberts (Ed.), Fish Pathology. Bailliere Tindall. London, pp289-291. Fryer, J. L., and Rohovec J. S. (1984) Principal bacterial diseases of cultured marine fish. Helgol. Meeresunters. 37, 5335-5345. Fukuda, Y. and Kusuda, R. (1981) Efficacy of vaccination for pseudotuberculosis in cultured yellowtail by various routes of administration. Bull Japan Soc Sci Fish, 47, 147–150 Fukuda Y, Kusuda R (1985) Vaccination of yellowtail against pseudotuberculosis. FishPath ol 20:421–425 Gravningen, K., Sakai, M., Mishiba, T., and Fujimoto, T. (2008) The efficacy and safety of an oil-based vaccine against Photobacterium damsela subsp. piscicida in yellowtail (Seriola quinqueradiata): A field study. Fish & Shellfish Immunology 24, 523-529. Gravningen, K., Thorarinsson, R., Johansen, L.H., Nissen, B., Rikardsen, K.S., Greger, E., and Vigneulle, M. (1998) Bivalent vaccines for sea bass (Dicentrachus labrax) against vibriosis and pasteurellosis. Journal of Applied Ichthyology 14, 159-162. Hamaguchi M and Kusuda R (1989) Field testing of Pasteurella piscicida formalin killed bacteria against pseudotuberculosis in cultured yellowtail, Seriola quinqueradiata. Bull Mar Sci Fish, Kochi Univ. 11, 11–16 Hawke, J.P., Plakas, S.M., Minton, R.V., McPhearson, R.M., Snider, T.G., and Guarino, A.M. (1987) Fish pasteurellosis of cultured striped bass (Morone saxatilis) in coastal Alabama. . Aquaculture 65, 193-204. Hirono, I., Kato, M., and Aoki, T. (1997) Identification of major antigenic proteins of Pasteurella piscicida. Microbial Pathogenesis 23, 371-380. Hjeltnes B. and Roberts R.J. (1993) Vibriosis. In: Inglis V, Roberts RJ, Bromage NR (eds) Bacterial diseases of fish. CambridgeUniversity Press, Cambridge Hoel, K. and Lillehaug, A. (1997) Adjuvant activity of polar glycopeptidolipids from Mycobacterium chelonae in experimental vaccines against Aeromonas salmonicida in salmonid fish. Fish Shellfish Immunol. 7, 365-376. Itami, T. and Kusada, R., (1980) Studies on spray vaccination against vibriosis in culturu ayu, II. Duration of vaccination efficacy and effect of different vaccine preparation. Bull. Jap. Soc. Sci. Fish., 46,366-703. Janssen, W.A., and Surgalla, M.J. (1968) Morphology, Physiology, and Serology of a Pasteurella Species Pathogenic for White Perch (Roccus americanus). J Bacteriol 96, 1606-1610. Jian, J., and Wu, Z. (2004) Influences of traditional Chinese medicine on non-specific immunity of Jian Carp (Cyprinus carpio var. Jian). Fish and Shellfish Immunology 16, 185-191. Kajita, Y., Sakai, M., Atsuta, S. and Kobayashi, M., (1990) The immunomodulatory effects of levamisole on rainbow trout. Oncorhynchus mykiss. Fish Pathol., 25, 93-98. Kawakami, H., Shinohara, N., Fukuda, Y., Yamashita, H., Kihara, H., and Sakai, M. (1997). The efficacy of lipopolysaccharide mixed chloroform-killed cell (LPS-CKC) bacterin of Pasteurella piscicida on Yellowtail, Seriola quinqueradiata. Aquaculture 154, 95-105. Kim, E., and Aoki, T. (1993) Drug resistance and broad geographical distribution of identical R plasmids of Pasteurella piscicida isolated from cultured yellowtail in Japan. Microbiology and Immunology 37, 103-109. Kim, E.H., and Aoki, T. (1996) Sequence analysis of the florfenicol resistance gene encoded in the transferable R-plasmid of a fish pathogen, Pasteurella piscicida. Microbiology and Immunology 40, 665-669. Kimura, T. and Kitao, T., (1971) On the causative agent of tuberculosis of yellowtail. Fish Pathol., 6, 8-14. Kitao, T., Yoshida, T., Kusuda, R., Matsuoka, T., Nakano, S., Okada, M. and Ooshima, T., (1992) In vitro antibacterial activity of bicozamycin against Pasteurella piscicida. Gyobyo Kenkyu, 27, 109-113. Koike, Y., Kuwahara, A., and Fujiwara, H. (1975) Characterization of Pasteurella piscicida isolated from white perch and cultivated yellowtail. Jpn J Microbiol 19, 241-247. Kusuda, R., and Hamaguchi, M. (1987) A comparative study on efficacy of immersion and combination of immersion and oral vaccination methods against pseudotuberculosis in yellowtail. Bulletin of the Japanese Society of Scientific Fisheries 53, 1005-1008. Kusuda, R., and Hamaguchi, M. (1988) The efficacy of attenuated live bacterin of Pasteurella piscicida against pseudotuberculosis in yellowtail. Bull Eur Ass Fish Pathol 8, 51-53. Kusuda R. and Inoue K., (1976) Studies on the application of ampicillin for pseudotuberculosis of cultured yellowtails. In vitro studies on sensitivity, development of drug-resistance and reversion of acquired drug-resistance characteristics of Pasteurella piscicida. Bull. Jap. Soc. Sci. Fish., 42, 969-973. Kusuda R., Salati F. (1993) Major bacterial diseases affecting mariculture in Japan. In: Faisal M, Hetrick FM (eds) Annual. review of fish diseases. Pergamon Press. New York, pp.69-85 Kusuda R, Ninomiya M, Hamaguchi M, Muraoka A (1988) The efficacy of ribosomal vaccine prepared from Pasteurella piscicida against pseudotuberculosis in cultured yellowtail. Fish Pathol 23, 191–196. Lee, K. K. and Ellis, A. E. (1990) Glycerophospholipid: cholesterol acyltransferase complexed with lipopolysaccharide (LPS) is a major lethal exotoxin and cytolysin of Aeromonas salmonicida: LPS stabilizes and enhances toxicity of the enzyme. J. Bacteriol., 172, 5385-5393. Lee, K. K. and Ellis, A. E. (1991) The role of the lethal extracellular cytolysin of Aeromonas salmonicida in the pathology of furunculosis. J. Fish Dis., 14, 453-460. Lin, J.H.-Y., Chen, T.-Y., Chen, M.-S., Chen, H.-E., Chou, R.-L., Chen, T.-I., Su, M.-S., and Yang, H.-L. (2006) Vaccination with three inactivated pathogens of cobia (Rachycentron canadum) stimulates protective immunity. Aquaculture 255, 125-132. Liu, P.C., Lin, J.I.Y., and Lee, K.K. (2003) Virulence of Photobacterium damselae subsp. piscicida in cultured cobia Rachycentron canadum. Journal of Basic Microbiology 43, 499-507. Liu, P. C., Tsai, C. N. and Lee, K. K. (2005) Development of bacterial vaccines and its efficacy in cage cultured cobia Rachycentron canadum. Fish Disease Research, 22,41-50. Lopez-Doriga, M.V., Barnes, A.C., Dos Santos, N.M.S., and Ellis, A.E. (2000) Invasion of fish epithelial cells by Photobacterium damselae suksp. piscicida : Evidence for receptor specificity, and effect of capsule and serum. Microbiology 146, 21-30. Magariños, B., Romalde, J.L., BANDIN, I., FOUZ, B., and Toranzo, A.E. (1992) Phenotypic, Antigenic, and Molecular Characterization of Pasteurella piscicida Strains Isolated from Fish. Applied and Environmental Microbiology 58, 3316-3322. Magariños, B., Toranzo, A.E., and Romalde, J.L. (1995) Different susceptibility of gilthead seabream and turbot to Pasteurella piscicida infection by the water route. Bull Eur Ass Fish Pathol 15, 88-90. Magariños, B., Toranzo, A.E., and Romalde, J.L. (1996) Phenotypic and pathobiological characteristics of Pasteurella piscicida. Annual Review of Fish Diseases 6, 41-64. Magarinos, B., Romalde, J.L., Noya, M., Barja, J.L., and Toranzo, A.E. (1996) Adherence and invasive capacities of the fish pathogen Pasteurella piscicida. FEMS Microbiology Letters 138, 29-34. Matsuoka, S., Wada, Y., Kawamoto, I., Naito, K., and Doi, S. (1990) Pasteurella piscicida infection in juvenile ayu (Plecoglossus altivelis). Fish Pathology 25, 253-254. Morinigo, M.A., Romalde, J.L., Chabrillon, M., Magarinos, B., Arijo, S., Balebona, M.C., and Toranzo, A.E. (2002) Effectiveness of a divalent vaccine for gilt-head sea bream (Sparus aurata) against Vibrio alginolyticus and Photobacterium damselae subsp. piscicida. Bulletin of the European Association of Fish Pathologists 22, 298-303. Morrison, R. N., Nowak, B. F. and Carson, J. (2000) The effect of levamisole as an adjuvant on the humoral immune response of Atlantic salmon, Salmo salar L. Bull. Eur. Ass. Fish Pathol., 20, 101-105. Mulvey, B., Landolt, M. L. and Busch, R. A., (1995) Effect of potassium aluminium sulphate (alum) used in an Aeromonas salmonicida bacterin on Atlantic salmon, Salmo salar L. J. Fish Dis., 19, 495-506. Munro, A. L. S., Hasting, T. S., Ellis. A. E. and Liversidge, J. (1980) Studies on an ichthyotoxic material produced extracellularly by the furnculosis bacterium, Aeromonas salmonicida. In: Proceedings of the third session of the EIPAC (FAO/OIE) CPRA: Fish Disease, pp: 98-106. Berlin: Springer-Verlag KG. Nelson, J.S., Kawahala, E., Kawai, K., and Kusuda, R. (1989) Macrophage infiltration in pseudotuberculosis of yellowtail, Seriola quinqueradiata. Bull Mar sci Fish kochi Univ 11, 17-22. Nitzan, S., Shwartsburd, B., and Heller, E.D. (2004) The effect of growth medium salinity of Photobacterium damselae subsp. piscicida on the immune response of hybrid bass (Morone saxatilis x M. chrysops). Fish and Shellfish Immunology 16, 107-116. Noya, M., Magariños, B., Toranzo, A.E., and Lamas, J. (1995) Sequential pathology ofexperimental pasteurellosis in gilthead seabream Sparus aurata. A light- and electron-microscopic study. Diseases of Aquatic Organisms 21, 177-186. Osorio, C.R., Collins, M.D., Toranzo, A.E., Barja, J.L., and Romalde, J.L. (1999) 16S rRNA Gene Sequence Analysis of Photobacterium damselae and Nested PCR Method for Rapid Detection of the Causative Agent of Fish Pasteurellosis. Appl Environ Microbiol 65, 2942-2946. Rahkonen, R., Salminen, M., Erkamo, E. (1997) Comparison fo the marine survival of Atlantic salmon, Salmo salar L. smolts vaccinated and nonvaccinated against vibriosis. Aquaculture, 156, 349-358. Rajan, P.R., Lin, J.H.-Y., Ho, M.-S., and Yang, H.-L. (2003) Simple and rapid detection of Photobacterium damselae ssp. piscicida by a PCR technique and plating method. Journal of Applied Microbiology 95, 1375-1380. Ramon G. (1925) Sur l’augmentation anormale de l’antitoxine chez les chevaux producteurs de serum antidiphterique. Bull Soc Centr Med Vet,101, 227–34. Ramon G. (1926) Procedes pour accro¨ıtre la production des antitoxins. Ann Inst Pasteur,40, 1–10. Ravelo, C., Magarinos, B., Herrero, M.C., Costa, L., Toranzo, A.E., and Romalde, J.L. (2006) Use of adjuvanted vaccines to lengthen the protection against lactococcosis in rainbow trout (Oncorhynchus mykiss). Aquaculture 251, 153-158. Robohm, R. (1983) Pasteurella piscicida (Lyon,France, Collection Foundation Marcel Merieux). Robohm, R. A., (1979) Pasteurella piscicida, the etiological agent of an epizootic in striped bass (Morone americanus) in Long Island Sound. 4th Annual Eastern Fish Health Workshop, Halifex, N. S., Canada. Romalde, J.L. (2002) Photobacterium damselae subsp. piscicida: An integrated view of a bacterial fish pathogen. International Microbiology 5, 3-9. Romalde, J.L., and Magarinos, B. (1997) Immunization with bacterial antigens: pasteurellosis. Developments in Biological Standardization 90, 167-177. Rombout, J.H.W.M., Berg, A.A., Berg, C.T.G.A., Witte, P., and Egberts, E. (1989) Immunological importance of the second gut segment of carp. III. Systemic and/or mucosal immune responses after immunization with soluble or particulate antigen. Journal of Fish Biology 35, 179-186. Sakai, M. (1999) Current research status of fish immunostimulants. Aquaculture 172, 63-92. Sano, M., Nakano, H., Kimura, T. and Kusuda, R. (1994) Therapeutic effect of fosfomicin on experimentally induced pseudotuberculosis in yellowtail. Fish Pathol., 29, 187-192. Santo, Y., Bandin, I., Nunez, S., Montero, M., Silva, A. and Toranzo, A. E. (1992) Comparison of the extracellular biological activities of Vibrio anguillarum and Aeromonas hydrophila. Aquaculture, 107, 259-270. Santos, Y., Romalde, J.L., Bandín, I., Magariños, B., Núnez, S., Barja, J.L., and Toranzo, A.E. (1993) Usefulness of the API-20E system for the identification of bacterial fish pathogens. Aquaculture 116, 111-120. Shao, B.-M., Xu, W., Dai, H., Tu, P., Li, Z., and Gao, X.-M. (2004) A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb. Biochemical and Biophysical Research Communications 320, 1103-1111. Simidu, U. and Egusa, S. (1972) A re-examination of the fish-pathogenic bacterium that had been reported as a Pasteurella species. Bull. Jap. Soc. Sci. Fish., 38, 803-812. Skarmeta, A.M., Bandin, I., Santos, Y., and Toranzo, A.E. (1995) In vitro killing of Pasteurella piscicida by fish macrophages. Diseases of Aquatic Organisms 23, 51-57. Smith P. D. (1982) Analysis of the hyperosomotic and bath methods for fish vaccination - Comparison of uptake of paritculate and non- particulate antigens. Dev. Comp. Immunol., 2, 181-186. Smith P. and Hiney M. (2000) Oil-adjuvanted furunculosis vaccines in commercial fish farms: a perliminary epizootiological investigation. Aquaculture, 190, 1-9. Snieszko, S.F., Bullock, G.L., Hollis, E., and Boone, J.G. (1964) Pasteurella sp. from an epizootic of white perch (Roccus americanus) in Chesapeake Bay tidewater areas. . Journal of Bacteriology 88, 1814-1815. Thune, R. L., Stanley, L. A. and Cooper, R. K. (1993) Pathogenesis of Gram-negative bacterial infections in warm-water fish. Annu. Rev. Fish. Dis., 37-68. Toranzo, A.E., Barreiro, S., Casal, J.F., Figueras, A., Magariiios, B., and Barja, J.L. (1991) Pasteurellosis in cultured gilthead seabream (Sparus aurata): first report in Spain. Aquaculture 99, 1-15. Toranzo, A. E. and Barja, J. L. (1993) Virulence factors of bacteria pathogenic for coldwater fish. Ann. Rev. Fish Dis., 3, 5-36. Tung, M.C., Tsai, S.S., Ho, L.F., Huang, S.T., and Chen, S.C. (1985) An acute septicemic infection of Pasteurella organism in pond-cultured Formosa snake-head fish (Channa maculata Lacepede) in Taiwan. Fish Pathology 20, 143-148. Velji, M. I., Albright, L. J. and Evelyn, T. P. T. (1992) Immunogenicity of various Vibrio ordalii lipopolysaccharide fractions in coho salmon, Oncorhynchus kisutch. Dis. Aquat. Org., 12, 97-101. Xu, H.D., You, C.G., Zhang, R.L., Gao, P., and Wang, Z.R. (2007) Effects of Astragalus Polysaccharides and Astragalosides on the Phagocytosis of Mycobacterium tuberculosis by macrophages. The Journal of International Medical Research 35, 84-90. Yuan, C., Pan, X., Gong, Y., Xia, A., Wu, G., Tang, J., and Han, X. (2008) Effects of Astragalus polysaccharides (APS) on the expression of immune response genes in head kidney, gill and spleen of the common carp, Cyprinus carpio L. International Immunopharmacology 8, 51-58. Zappulli, V., Patarnello, T., Patarnello, P., Frassineti, F., Franch, R., Manfrin, A., Castagnaro, M., and Bargelloni, L. (2005) Direct identification of Photobacterium damselae subspecies piscicida by PCR-RFLP analysis. Diseases of Aquatic Organisms 65, 53-61. Zorrilla, I., Balebona, M.C., Morinigo, M.A., Sarasquete, C., and Borrego, J.J. (1999) Isolation and characterization of the causative agent of pasteurellosis, Photobacterium damsela ssp. piscicida, from sole, Solea senegalensis (Kaup). Journal of Fish Diseases 22, 167-172. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/41471 | - |
dc.description.abstract | 巴斯德桿菌(Photobacterium damselae subsp. Piscicida, Pdp)歷年都是造成台灣海鱺苗在春夏季移入箱網期間大量死亡的主因。疫苗是預防細菌性疾病的重要防疫策略,本研究則在探討Pdp疫苗免疫策略中的一些因子,包括去活化藥劑、免疫劑量、佐劑、追加免疫及免疫激活劑等,對疫苗誘發海鱺專一性免疫力及抗 Pdp感染力之影響。實驗結果顯示,0.5%福馬林能完全去活化 Pdp,且比相同濃度之β-propiolacton對抗原性的破壞小。福馬林去活化之Pdp疫苗,以0.333 mg/g fish 免疫劑量所誘發之專一性抗體力價,明顯高於0.006 mg/g fish組。比較魚油、沙拉油或氫氧化鋁佐劑對Pdp疫苗誘發專一性抗體力價之影響,氫氧化鋁佐劑效果最好,但抗體力價持續時間相對短。對15 g重之海鱺苗進行一次及追加免疫,結果追加免疫後抗體力價高峰產生的時間縮短,且力價明顯升高,顯示15 g重之海鱺苗已有免疫記憶力,但高力價維持時間短。在Pdp菌體疫苗中添加入去活化之Pdp胞外產物,其免疫效果和未添加組比較並無顯著差異。比較魚用ISA 763A油性佐劑和水包油包水(water in oil in water, WOW)製備的Pdp疫苗在追加免疫後所引起的專一性抗體力價,ISA佐劑組的免疫時效比WOW組長,且ISA佐劑組攻毒後死亡率比WOW組低。Pdp疫苗中添加鰻弧菌(Vibrio anguillarum)及黃耆,一次免疫後,添加黃耆之疫苗組在浸泡攻毒後的保護效果最好,但在注射攻毒試驗中則低於無添加組,推測黃耆可能有助於非專一性黏液免疫而降低Pdp感染;去活化鰻弧菌的添加對 Pdp專一性免疫或保護效果則無促進效果,且可能有干擾的反效果。 | zh_TW |
dc.description.abstract | Photobacterium damselae subsp. Piscicida (Pdp) has been a major pathogen causing serious mortality of cultured cobia in the past years in Taiwan, particularly when they are moved into sea cage. Vaccination is a fundamental strategy to prevent the outbreak of bacterial diseases. In this study, several immune program factors of Pdp vaccine, including inactivated agent, immune dosage, adjuvant, boost immune, and immunostimulant were investigated to verify the influences on inducing specific immunity and the ability against Pdp infection in cobia. Our results revealed that 0.5% formalin could completely inactivate Pdp and retain higher antigenicity than 0.5% β-propiolactone did. The specific antibody titer caused by high dosage antigen (0.333 mg/g) was higher than low dosage (0.006 mg/g). Compare the influences on inducing specific antibody titer of different adjuvants in Pdp vaccines, aluminum hydroxide exhibited the highest immune effect among all adjuvants, although with a relatively shorter duration. Due to the low memory of cobia immunity, a second vaccination was required to enhance the immunity efficacy and protection period. No significant differences were observed between Pdp vaccine with or without adding inactivated extracellular product. Compare the antibody titer induced by Pdp vaccine with ISA 763A adjuvant or with fish oil adjuvant embedded as water in oil in water (WOW group), the duration of ISA group was longer than WOW group after boost. Besides, the cumulative mortality of ISA group was 40% lower than negative control (100%) after intraperitoneal challenge. Cobia immunized by Pdp vaccine was supplemented in Vibrio anguillarum (V.ang) and Astragalus polysaccharide (APS) showed the best protection efficacy after immersion challenge. However, the cumulative mortality was higher than that without APS after intraperitoneal challenge. It is suggested that APS may lower Pdp infection by inducing non-specific immunity in mucus. There is no enhancement on specific immunity and protection effect when inactivated V.ang was supplemented in Pdp vaccine ; contrarily it seems to interfere Pdp vaccine protection efficacy. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T00:20:08Z (GMT). No. of bitstreams: 1 ntu-98-R95b41028-1.pdf: 1139379 bytes, checksum: e6ff71262def616640d85abf1b139bff (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 中文摘要….………………………………………………………… I
英文摘要….………………………………………………………… II 目錄….……………………………………………………………… III 1、文獻回顧 1-1海鱺…………………………………………………………… 1 1-2巴斯德桿菌…………………………………………………… 3 1-3巴斯德桿菌症………………………………………………… 6 1-4魚類細菌性疾病之防治……………………………………… 6 1-5魚用疫苗……………………………………………………… 8 1-6佐劑…………………………………………………………… 12 1-7免疫激活劑…………………………………………………… 13 1-8研究目的...…………………………………………………… 14 2、實驗材料與方法 2-1實驗魚來源…………………………………………………… 15 2-2菌株來源……………………………………………………… 15 2-3菌株之採樣、分離與保存…………………………………… 15 2-4菌株鑑定……………………………………………………… 15 2-5菌株毒性試驗………………… ……………………………… 17 2-6疫苗製備……………………………………………………… 17 2-7抗血清之製備………………………………………………… 18 2-8抗體力價分析………………………………………………… 18 2-9免疫試驗……………………………………………………… 19 2-9-1去活化藥劑及免疫劑量對誘發專一性抗體力價之影響… 19 2-9-2一次免疫與追加免疫對抗體力價與疫苗保護效果之影響 19 2-9-3不同佐劑與Pdp胞外產物對疫苗誘發抗體之影響……… 20 2-9-4商業魚用佐劑及疫苗包埋方式對免疫效果之影響……… 20 2-9-5單價疫苗、雙價疫苗及添加黃耆之單價疫苗對免疫效果之影響…………………………………………………………………… 21 3、實驗結果 3-1巴斯德桿菌菌株鑑定………………………………………… 23 3-2菌株毒性試驗………………………………………………… 23 3-3去活化藥劑及免疫劑量對誘發專一性抗體力價之影響…… 23 3-4一次免疫與追加免疫對抗體力價與疫苗保護效果之影響… 24 3-5不同佐劑與Pdp胞外產物對疫苗誘發抗體之影響………… 24 3-6商業魚用佐劑及疫苗包埋方式對免疫效果之影響………… 25 3-7單價疫苗、雙價疫苗及添加黃耆之單價疫苗對免疫效果之影響…………………………………………………………………… 26 4、討論…………………………………………………………… 28 References………………………………………………………… 35 Figures and Tables……………………………………………… 49 附錄………………………………………………………………… 66 | |
dc.language.iso | zh-TW | |
dc.title | 巴斯德桿菌去活化疫苗不同免疫策略對海鱺專一性免疫反應之影響 | zh_TW |
dc.title | The impacts of different immunization strategies of inactivated Photobacterium damselae subsp. piscicida on cobia specific immune response | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 宋延齡,陳哲俊,王俊順 | |
dc.subject.keyword | 巴斯德桿菌,細菌疫苗,海鱺,專一性免疫, | zh_TW |
dc.subject.keyword | Photobacterium damselae subsp. piscicida,bacterial vaccine,cobia,specific immune response, | en |
dc.relation.page | 68 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2009-02-11 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-98-1.pdf 目前未授權公開取用 | 1.11 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。