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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 王惠鈞(Andrew H.-J. Wang) | |
| dc.contributor.author | Yu-Wei Chang | en |
| dc.contributor.author | 張育瑋 | zh_TW |
| dc.date.accessioned | 2021-06-14T17:20:03Z | - |
| dc.date.available | 2010-07-27 | |
| dc.date.copyright | 2008-07-27 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-07-24 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/41148 | - |
| dc.description.abstract | Homologous recombination is a universal mechanism for repairing DNA double strand breaks (DSBs) and injured DNA replication forks. RecA family proteins play a central role in homologous recombination by forming nucleoprotein filaments. These proteins include the prokaryotic RecA, archaeal RadA, and eukaryotic Rad51 and Dmc1. Here we report three crystal structures of the Sulfolobus solfataricus RadA. All of these structures are packed in the orthorhombic lattice and form the left-handed helical filaments with different helical pitches. The results strongly suggest the universal existence of left-handed helical filaments of the RecA family proteins. The structural variations of alternate protein-protein interfaces not only exhibit the extreme flexibility of filaments but also demonstrate the asymmetry within the filaments, implying the sequential binding of DNA and hydrolysis of ATP by RecA family proteins. Further structural analyses and subsequent mutagenesis studies, coupled with biochemical assays, offer a novel insight into the mechanism controlling the SsoRadA quaternary structures and filament assemblies during the catalysis of strand exchange, which involves Asp 70 and Arg 72 in the N-terminus of the NTD-CTD hinge. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-14T17:20:03Z (GMT). No. of bitstreams: 1 ntu-97-R95b46010-1.pdf: 8383486 bytes, checksum: 4fb8ce1f16d3e8b721a43dc8967ba509 (MD5) Previous issue date: 2008 | en |
| dc.description.tableofcontents | 誌謝 i
中文摘要 ii ABSTRACT iii CONTENTS iv LIST OF FIGURES vi LIST OF TABLES vii INTRODUCTION 1 MATERIALS AND METHODS .............................................................. 6 Expression and Purification of SsoRadA 6 Crystallization, Data Collection and Structure Determination of SsoRadA 7 DNA Substrates 8 DNA Binding Assay 9 ssDNA-stimulated ATPase Activity Assays 9 Strand Assimilation Assays 10 RESULTS 11 Overall Structures of SsoRadA 0522, 0719, and 1007 11 The Asymmetry of SsoRadA Protein Filaments 12 The N-terminus of the NTD-CTD Hinge Accounts for Different Orientation of NTDs 14 Analyses of Point Mutations Indicate that SsoRadA Asp70 and Arg72 Play Crucial Roles in Strand Paring Reaction 16 DISCUSSION 20 The Orthorhombic Crystals of Left-handed RecA Family Protein Filaments 20 Implication by the Asymmetry in the Filaments 21 The Effects of DNA on SsoRadA 22 Structural Analysis Combining Biochemical Assays Indicates that D70 and R72 are Involved in Controlling the Quaternary Structures of SsoRadA Filaments 24 The Limitation of the Resolution of RadA/Rad51 Crystal Structures 27 CONCLUSION 28 FIGURES 30 TABLES 48 REFERENCES 52 POSTER 60 APPENDIX 62 | |
| dc.language.iso | en | |
| dc.subject | RADA | zh_TW |
| dc.subject | 斜方晶系晶體結構 | zh_TW |
| dc.subject | RECA | zh_TW |
| dc.subject | 同源重組 | zh_TW |
| dc.subject | 左手旋蛋白質絲狀聚合體 | zh_TW |
| dc.subject | 蛋白質絲狀聚合體的組合 | zh_TW |
| dc.subject | filament assembly | en |
| dc.subject | Homologous recombination | en |
| dc.subject | RadA | en |
| dc.subject | orthorhombic crystal structures | en |
| dc.subject | left-handed protein filaments | en |
| dc.subject | RecA family proteins | en |
| dc.title | Sulfolobus solfataricus RadA 的晶體結構:
三種新構型及其對同源重組的啟發 | zh_TW |
| dc.title | Crystal Structure of Sulfolobus solfataricus RadA:
Three New Conformations and their Implications in Homologous Recombination | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王廷方(Ting-Fang Wang),史有伶(Yu-Ling Shih) | |
| dc.subject.keyword | 同源重組,RECA,RADA,斜方晶系晶體結構,左手旋蛋白質絲狀聚合體,蛋白質絲狀聚合體的組合, | zh_TW |
| dc.subject.keyword | Homologous recombination,RecA family proteins,RadA,orthorhombic crystal structures,left-handed protein filaments,filament assembly, | en |
| dc.relation.page | 63 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2008-07-27 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| Appears in Collections: | 生化科學研究所 | |
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| ntu-97-1.pdf Restricted Access | 8.19 MB | Adobe PDF |
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