請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/40623完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鄭秋萍 | |
| dc.contributor.author | Yong-Yi Chen | en |
| dc.contributor.author | 陳永義 | zh_TW |
| dc.date.accessioned | 2021-06-14T16:53:39Z | - |
| dc.date.available | 2013-08-06 | |
| dc.date.copyright | 2008-08-06 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-07-29 | |
| dc.identifier.citation | Ahn, C.S., Lee, J.H., Reum Hwang, A., Kim, W.T., and Pai, H.S. (2006). Prohibitin is involved in mitochondrial biogenesis in plants. Plant J 46, 658-667.
Barkai-Golan, R., Mirelman, D., and Sharon, N. (1978). Studies on growth inhibition by lectins of Penicillia and Aspergilli. Arch Microbiol 116, 119-121. Beckers, G.J., and Spoel, S.H. (2006). Fine-Tuning Plant Defence Signalling: Salicylate versus Jasmonate. Plant Biol (Stuttg) 8, 1-10. Benedito, V.A., Visser, P.B., Angenent, G.C., and Krens, F.A. (2004). The potential of virus-induced gene silencing for speeding up functional characterization of plant genes. Genet Mol Res 3, 323-341. Bhattarai, K.K., Li, Q., Liu, Y., Dinesh-Kumar, S.P., and Kaloshian, I. (2007). The MI-1-mediated pest resistance requires Hsp90 and Sgt1. Plant Physiol 144, 312-323. Birnboim, H.C., and Doly, J. (1979). A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7, 1513-1523. Bostock, R.M. (2005). Signal crosstalk and induced resistance: straddling the line between cost and benefit. Annu Rev Phytopathol 43, 545-580. Braz, A.S., Finnegan, J., Waterhouse, P., and Margis, R. (2004). A plant orthologue of RNase L inhibitor (RLI) is induced in plants showing RNA interference. J Mol Evol 59, 20-30. Brigneti, G., Martin-Hernandez, A.M., Jin, H., Chen, J., Baulcombe, D.C., Baker, B., and Jones, J.D. (2004). Virus-induced gene silencing in Solanum species. Plant J 39, 264-272. Burch-Smith, T.M., Anderson, J.C., Martin, G.B., and Dinesh-Kumar, S.P. (2004). Applications and advantages of virus-induced gene silencing for gene function studies in plants. Plant J 39, 734-746. Burch-Smith, T.M., Schiff, M., Liu, Y., and Dinesh-Kumar, S.P. (2006). Efficient virus-induced gene silencing in Arabidopsis. Plant Physiol 142, 21-27. Calderini, O., Glab, N., Bergounioux, C., Heberle-Bors, E., and Wilson, C. (2001). A novel tobacco mitogen-activated protein (MAP) kinase kinase, NtMEK1, activates the cell cycle-regulated p43Ntf6 MAP kinase. J Biol Chem 276, 18139-18145. Carmeille, A., Caranta, C., Dintinger, J., Prior, P., Luisetti, J., and Besse, P. (2006). Identification of QTLs for Ralstonia solanacearum race 3-phylotype II resistance in tomato. Theor Appl Genet 113, 110-121. Casasoli, M., Spadoni, S., Lilley, K.S., Cervone, F., De Lorenzo, G., and Mattei, B. (2008). Identification by 2-D DIGE of apoplastic proteins regulated by oligogalacturonides in Arabidopsis thaliana. Proteomics 8, 1042-1054. Chan, Y.L., Prasad, V., Sanjaya, Chen, K.H., Liu, P.C., Chan, M.T., and Cheng, C.P. (2005). Transgenic tomato plants expressing an Arabidopsis thionin (Thi2.1) driven by fruit-inactive promoter battle against phytopathogenic attack. Planta 221, 386-393. Chen, J.C., Jiang, C.Z., Gookin, T.E., Hunter, D.A., Clark, D.G., and Reid, M.S. (2004). Chalcone synthase as a reporter in virus-induced gene silencing studies of flower senescence. Plant Mol Biol 55, 521-530. Clarke, J.D., Volko, S.M., Ledford, H., Ausubel, F.M., and Dong, X. (2000). Roles of salicylic acid, jasmonic acid, and ethylene in cpr-induced resistance in Arabidopsis. Plant Cell 12, 2175-2190. del Pozo, O., Pedley, K.F., and Martin, G.B. (2004). MAPKKKalpha is a positive regulator of cell death associated with both plant immunity and disease. EMBO J 23, 3072-3082. Denny, T.P. (2006). Plant pathogenic Ralstonia species. In Plant-Associated Bacteria, S.S. Gnanamanickam, ed (Dordrecht, The Netherlands: Springer Publishing), pp. 573-644. Deslandes, L., Olivier, J., Theulieres, F., Hirsch, J., Feng, D.X., Bittner-Eddy, P., Beynon, J., and Marco, Y. (2002). Resistance to Ralstonia solanacearum in Arabidopsis thaliana is conferred by the recessive RRS1-R gene, a member of a novel family of resistance genes. Proc Natl Acad Sci U S A 99, 2404-2409. Deslandes, L., Olivier, J., Peeters, N., Feng, D.X., Khounlotham, M., Boucher, C., Somssich, I., Genin, S., and Marco, Y. (2003). Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus. Proc Natl Acad Sci U S A 100, 8024-8029. Dong, J., Lai, R., Nielsen, K., Fekete, C.A., Qiu, H., and Hinnebusch, A.G. (2004). The essential ATP-binding cassette protein RLI1 functions in translation by promoting preinitiation complex assembly. J Biol Chem 279, 42157-42168. Dong, Y., Burch-Smith, T.M., Liu, Y., Mamillapalli, P., and Dinesh-Kumar, S.P. (2007). A ligation-independent cloning tobacco rattle virus vector for high-throughput virus-induced gene silencing identifies roles for NbMADS4-1 and -2 in floral development. Plant Physiol 145, 1161-1170. Ekengren, S.K., Liu, Y., Schiff, M., Dinesh-Kumar, S.P., and Martin, G.B. (2003). Two MAPK cascades, NPR1, and TGA transcription factors play a role in Pto-mediated disease resistance in tomato. Plant J 36, 905-917. Eulgem, T., and Somssich, I.E. (2007). Networks of WRKY transcription factors in defense signaling. Curr Opin Plant Biol 10, 366-371. Ferrari, S., Plotnikova, J.M., De Lorenzo, G., and Ausubel, F.M. (2003). Arabidopsis local resistance to Botrytis cinerea involves salicylic acid and camalexin and requires EDS4 and PAD2, but not SID2, EDS5 or PAD4. Plant J 35, 193-205. Fitches, E., Edwards, M.G., Mee, C., Grishin, E., Gatehouse, A.M., Edwards, J.P., and Gatehouse, J.A. (2004). Fusion proteins containing insect-specific toxins as pest control agents: snowdrop lectin delivers fused insecticidal spider venom toxin to insect haemolymph following oral ingestion. J Insect Physiol 50, 61-71. Fu, D.Q., Zhu, B.Z., Zhu, H.L., Jiang, W.B., and Luo, Y.B. (2005). Virus-induced gene silencing in tomato fruit. Plant J 43, 299-308. Glazebrook, J. (2005). Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annu Rev Phytopathol 43, 205-227. Godge, M.R., Purkayastha, A., Dasgupta, I., and Kumar, P.P. (2008). Virus-induced gene silencing for functional analysis of selected genes. Plant Cell Rep 27, 209-219. Gould, B., and Kramer, E.M. (2007). Virus-induced gene silencing as a tool for functional analyses in the emerging model plant Aquilegia (columbine, Ranunculaceae). Plant Methods 3, 6. Guo, X., and Stotz, H.U. (2007). Defense against Sclerotinia sclerotiorum in Arabidopsis is dependent on jasmonic acid, salicylic acid, and ethylene signaling. Mol Plant Microbe Interact 20, 1384-1395. Gupta, V., Willits, M.G., and Glazebrook, J. (2000). Arabidopsis thaliana EDS4 contributes to salicylic acid (SA)-dependent expression of defense responses: evidence for inhibition of jasmonic acid signaling by SA. Mol Plant Microbe Interact 13, 503-511. Hanahan, D. (1983). Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166, 557-580. Hernandez-Blanco, C., Feng, D.X., Hu, J., Sanchez-Vallet, A., Deslandes, L., Llorente, F., Berrocal-Lobo, M., Keller, H., Barlet, X., Sanchez-Rodriguez, C., Anderson, L.K., Somerville, S., Marco, Y., and Molina, A. (2007). Impairment of cellulose synthases required for Arabidopsis secondary cell wall formation enhances disease resistance. Plant Cell 19, 890-903. Hileman, L.C., Drea, S., Martino, G., Litt, A., and Irish, V.F. (2005). Virus-induced gene silencing is an effective tool for assaying gene function in the basal eudicot species Papaver somniferum (opium poppy). Plant J 44, 334-341. Hirsch, J., Deslandes, L., Feng, D.X., Balague, C., and Marco, Y. (2002). Delayed Symptom Development in ein2-1, an Arabidopsis Ethylene-Insensitive Mutant, in Response to Bacterial Wilt Caused by Ralstonia solanacearum. Phytopathology 92, 1142-1148. Hogervorst, P.A., Ferry, N., Gatehouse, A.M., Wackers, F.L., and Romeis, J. (2006). Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion. J Insect Physiol 52, 614-624. Hwang, K.Y., Chou, I.C., Lin, Y.M., and Cheng, C.P. (2004). Plant age-related resistance to Ralstonia solanacearum, the causal agent of bacterial wilt. J Gene Mol Biol 15, 108-115. Jin, H., Liu, Y., Yang, K.Y., Kim, C.Y., Baker, B., and Zhang, S. (2003). Function of a mitogen-activated protein kinase pathway in N gene-mediated resistance in tobacco. Plant J 33, 719-731. Kajaste-Rudnitski, A., Mashimo, T., Frenkiel, M.P., Guenet, J.L., Lucas, M., and Despres, P. (2006). The 2',5'-oligoadenylate synthetase 1b is a potent inhibitor of West Nile virus replication inside infected cells. J Biol Chem 281, 4624-4637. Katou, S., Yoshioka, H., Kawakita, K., Rowland, O., Jones, J.D., Mori, H., and Doke, N. (2005). Involvement of PPS3 phosphorylated by elicitor-responsive mitogen-activated protein kinases in the regulation of plant cell death. Plant Physiol 139, 1914-1926. Kiba, A., Tomiyama, H., Takahashi, H., Hamada, H., Ohnishi, K., Okuno, T., and Hikichi, Y. (2003). Induction of resistance and expression of defense-related genes in tobacco leaves infiltrated with Ralstonia solanacearum. Plant Cell Physiol 44, 287-295. Klessig, D.F., Durner, J., Noad, R., Navarre, D.A., Wendehenne, D., Kumar, D., Zhou, J.M., Shah, J., Zhang, S., Kachroo, P., Trifa, Y., Pontier, D., Lam, E., and Silva, H. (2000). Nitric oxide and salicylic acid signaling in plant defense. Proc Natl Acad Sci U S A 97, 8849-8855. Koornneef, A., and Pieterse, C.M. (2008). Cross talk in defense signaling. Plant Physiol 146, 839-844. Kus, J.V., Zaton, K., Sarkar, R., and Cameron, R.K. (2002). Age-related resistance in Arabidopsis is a developmentally regulated defense response to Pseudomonas syringae. Plant Cell 14, 479-490. Lahaye, T. (2004). Illuminating the molecular basis of gene-for-gene resistance; Arabidopsis thaliana RRS1-R and its interaction with Ralstonia solanacearum popP2. Trends Plant Sci 9, 1-4. Li, Q., Xie, Q.G., Smith-Becker, J., Navarre, D.A., and Kaloshian, I. (2006). Mi-1-Mediated aphid resistance involves salicylic acid and mitogen-activated protein kinase signaling cascades. Mol Plant Microbe Interact 19, 655-664. Lin, W.C., Lu, C.F., Wu, J.W., Cheng, M.L., Lin, Y.M., Yang, N.S., Black, L., Green, S.K., Wang, J.F., and Cheng, C.P. (2004). Transgenic tomato plants expressing the Arabidopsis NPR1 gene display enhanced resistance to a spectrum of fungal and bacterial diseases. Transgenic Res 13, 567-581. Lin, Y.M., Chou, I.C., Wang, J.F., Ho, F.I., Chu, Y.J., Huang, P.C., Lu, D.K., Shen, H.L., Elbaz, M., Huang, S.M., and Cheng, C.P. (2008). Transposon Mutagenesis Reveals Differential Pathogenesis of Ralstonia solanacearum on Tomato and Arabidopsis. Mol Plant Microbe Interact 21, in press. Liu, E., and Page, J.E. (2008). Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virus. Plant Methods 4, 5. Liu, Y., Schiff, M., and Dinesh-Kumar, S.P. (2002). Virus-induced gene silencing in tomato. Plant J 31, 777-786. Majumder, P., Banerjee, S., and Das, S. (2004). Identification of receptors responsible for binding of the mannose specific lectin to the gut epithelial membrane of the target insects. Glycoconj J 20, 525-530. Menke, F.L., Kang, H.G., Chen, Z., Park, J.M., Kumar, D., and Klessig, D.F. (2005). Tobacco transcription factor WRKY1 is phosphorylated by the MAP kinase SIPK and mediates HR-like cell death in tobacco. Mol Plant Microbe Interact 18, 1027-1034. Miao, Y., Laun, T.M., Smykowski, A., and Zentgraf, U. (2007). Arabidopsis MEKK1 can take a short cut: it can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter. Plant Mol Biol 65, 63-76. Nurnberger, T., Brunner, F., Kemmerling, B., and Piater, L. (2004). Innate immunity in plants and animals: striking similarities and obvious differences. Immunol Rev 198, 249-266. Orzaez, D., Mirabel, S., Wieland, W.H., and Granell, A. (2006). Agroinjection of tomato fruits. A tool for rapid functional analysis of transgenes directly in fruit. Plant Physiol 140, 3-11. Panter, S.N., Hammond-Kosack, K.E., Harrison, K., Jones, J.D., and Jones, D.A. (2002). Developmental control of promoter activity is not responsible for mature onset of Cf-9B-mediated resistance to leaf mold in tomato. Mol Plant Microbe Interact 15, 1099-1107. Pedley, K.F., and Martin, G.B. (2004). Identification of MAPKs and their possible MAPK kinase activators involved in the Pto-mediated defense response of tomato. J Biol Chem 279, 49229-49235. Pedley, K.F., and Martin, G.B. (2005). Role of mitogen-activated protein kinases in plant immunity. Curr Opin Plant Biol 8, 541-547. Pieterse, C.M., and Dicke, M. (2007). Plant interactions with microbes and insects: from molecular mechanisms to ecology. Trends Plant Sci 12, 564-569. Player, M.R., Wondrak, E.M., Bayly, S.F., and Torrence, P.F. (1998). Ribonuclease L, a 2-5A-dependent enzyme: purification to homogeneity and assays for 2-5A binding and catalytic activity. Methods 15, 243-253. Pontier, D., Tronchet, M., Rogowsky, P., Lam, E., and Roby, D. (1998). Activation of hsr203, a plant gene expressed during incompatible plant-pathogen interactions, is correlated with programmed cell death. Mol Plant Microbe Interact 11, 544-554. Ratcliff, F., Martin-Hernandez, A.M., and Baulcombe, D.C. (2001). Technical Advance. Tobacco rattle virus as a vector for analysis of gene function by silencing. Plant J 25, 237-245. Robatzek, S., Bittel, P., Chinchilla, D., Kochner, P., Felix, G., Shiu, S.H., and Boller, T. (2007). Molecular identification and characterization of the tomato flagellin receptor LeFLS2, an orthologue of Arabidopsis FLS2 exhibiting characteristically different perception specificities. Plant Mol Biol 64, 539-547. Robert-Seilaniantz, A., Navarro, L., Bari, R., and Jones, J.D. (2007). Pathological hormone imbalances. Curr Opin Plant Biol 10, 372-379. Sarmiento, C., Nigul, L., Kazantseva, J., Buschmann, M., and Truve, E. (2006). AtRLI2 is an endogenous suppressor of RNA silencing. Plant Mol Biol 61, 153-163. Scott, J.W., Wang, J.F., and Hanson, P.M. (2005). Breeding tomatoes for resistance to bacterial wilt, a Global view. In Proceeding of 1st International Symposium on Tomato Diseases, T. Momol, P. Ji, and J.B. Jones, eds (Acta Hort), pp. 161-172. Senthil-Kumar, M., and Udayakumar, M. (2006). High-throughput virus-induced gene-silencing approach to assess the functional relevance of a moisture stress-induced cDNA homologous to lea4. J Exp Bot 57, 2291-2302. Senthil-Kumar, M., Govind, G., Kang, L., Mysore, K.S., and Udayakumar, M. (2007a). Functional characterization of Nicotiana benthamiana homologs of peanut water deficit-induced genes by virus-induced gene silencing. Planta 225, 523-539. Senthil-Kumar, M., Hema, R., Anand, A., Kang, L., Udayakumar, M., and Mysore, K.S. (2007b). A systematic study to determine the extent of gene silencing in Nicotiana benthamiana and other Solanaceae species when heterologous gene sequences are used for virus-induced gene silencing. New Phytol 176, 782-791. Sharon, N., and Lis, H. (2004). History of lectins: from hemagglutinins to biological recognition molecules. Glycobiology 14, 53R-62R. Soyano, T., Nishihama, R., Morikiyo, K., Ishikawa, M., and Machida, Y. (2003). NQK1/NtMEK1 is a MAPKK that acts in the NPK1 MAPKKK-mediated MAPK cascade and is required for plant cytokinesis. Genes Dev 17, 1055-1067. Spitzer, B., Zvi, M.M., Ovadis, M., Marhevka, E., Barkai, O., Edelbaum, O., Marton, I., Masci, T., Alon, M., Morin, S., Rogachev, I., Aharoni, A., and Vainstein, A. (2007). Reverse genetics of floral scent: application of tobacco rattle virus-based gene silencing in Petunia. Plant Physiol 145, 1241-1250. Spoel, S.H., Johnson, J.S., and Dong, X. (2007). Regulation of tradeoffs between plant defenses against pathogens with different lifestyles. Proc Natl Acad Sci U S A 104, 18842-18847. Spoel, S.H., Koornneef, A., Claessens, S.M., Korzelius, J.P., Van Pelt, J.A., Mueller, M.J., Buchala, A.J., Metraux, J.P., Brown, R., Kazan, K., Van Loon, L.C., Dong, X., and Pieterse, C.M. (2003). NPR1 modulates cross-talk between salicylate- and jasmonate-dependent defense pathways through a novel function in the cytosol. Plant Cell 15, 760-770. Stulemeijer, I.J., Stratmann, J.W., and Joosten, M.H. (2007). Tomato mitogen-activated protein kinases LeMPK1, LeMPK2, and LeMPK3 are activated during the Cf-4/Avr4-induced hypersensitive response and have distinct phosphorylation specificities. Plant Physiol 144, 1481-1494. Subramanyam, S., Smith, D.F., Clemens, J.C., Webb, M.A., Sardesai, N., and Williams, C.E. (2008). Functional Characterization of HFR1, a High-Mannose N-Glycan-Specific Wheat Lectin Induced by Hessian Fly Larvae. Plant Physiol 147, 1412-1426. Takahashi, Y., Uehara, Y., Berberich, T., Ito, A., Saitoh, H., Miyazaki, A., Terauchi, R., and Kusano, T. (2004). A subset of hypersensitive response marker genes, including HSR203J, is the downstream target of a spermine signal transduction pathway in tobacco. Plant J 40, 586-595. Tena, G., Asai, T., Chiu, W.L., and Sheen, J. (2001). Plant mitogen-activated protein kinase signaling cascades. Curr Opin Plant Biol 4, 392-400. Thomas, C.L., Jones, L., Baulcombe, D.C., and Maule, A.J. (2001). Size constraints for targeting post-transcriptional gene silencing and for RNA-directed methylation in Nicotiana benthamiana using a potato virus X vector. Plant J 25, 417-425. Valentine, T., Shaw, J., Blok, V.C., Phillips, M.S., Oparka, K.J., and Lacomme, C. (2004). Efficient virus-induced gene silencing in roots using a modified tobacco rattle virus vector. Plant Physiol 136, 3999-4009. Van Damme, E.J., Nakamura-Tsuruta, S., Smith, D.F., Ongenaert, M., Winter, H.C., Rouge, P., Goldstein, I.J., Mo, H., Kominami, J., Culerrier, R., Barre, A., Hirabayashi, J., and Peumans, W.J. (2007). Phylogenetic and specificity studies of two-domain GNA-related lectins: generation of multispecificity through domain duplication and divergent evolution. Biochem J 404, 51-61. van Loon, L.C., Rep, M., and Pieterse, C.M. (2006). Significance of inducible defense-related proteins in infected plants. Annu Rev Phytopathol 44, 135-162. van Wees, S.C., de Swart, E.A., van Pelt, J.A., van Loon, L.C., and Pieterse, C.M. (2000). Enhancement of induced disease resistance by simultaneous activation of salicylate- and jasmonate-dependent defense pathways in Arabidopsis thaliana. Proc Natl Acad Sci U S A 97, 8711-8716. Vasse, J., Genin, S., Frey, P., Boucher, C., and Brito, B. (2000). The hrpB and hrpG regulatory genes of Ralstonia solanacearum are required for different stages of the tomato root infection process. Mol Plant Microbe Interact 13, 259-267. Wang, D., Weaver, N.D., Kesarwani, M., and Dong, X. (2005). Induction of protein secretory pathway is required for systemic acquired resistance. Science 308, 1036-1040. Wang, J.F., and Lin, C.H. (2005). Colonization capacity of Ralstonia solanacearum tomato strains differing in aggressiveness on tomatoes and weeds. In Bacterial Wilt Disease and the Ralstonia solanacearum Species Complex, C. Allen, P. Prior, and A.C. Hayward, eds (St Paul: APS Press), pp. 73-79. Wang, J.F., Milosveski, V., Schramek, C., Fong, G.H., Becks, G.P., and Hill, D.J. (1998). Presence and possible role of vascular endothelial growth factor in thyroid cell growth and function. J Endocrinol 157, 5-12. Wang, J.F., Olivier, J., Thoquet, P., Mangin, B., Sauviac, L., and Grimsley, N.H. (2000). Resistance of tomato line Hawaii7996 to Ralstonia solanacearum Pss4 in Taiwan is controlled mainly by a major strain-specific locus. Mol Plant Microbe Interact 13, 6-13. Wang, Y., Liu, C., Li, K., Sun, F., Hu, H., Li, X., Zhao, Y., Han, C., Zhang, W., Duan, Y., and Liu, M. (2007). Arabidopsis EIN2 modulates stress response through abscisic acid response pathway. Plant Mol Biol 64, 633-644. Wege, S., Scholz, A., Gleissberg, S., and Becker, A. (2007). Highly efficient virus-induced gene silencing (VIGS) in California poppy (Eschscholzia californica): an evaluation of VIGS as a strategy to obtain functional data from non-model plants. Ann Bot (Lond) 100, 641-649. Wei, C.F., Kvitko, B.H., Shimizu, R., Crabill, E., Alfano, J.R., Lin, N.C., Martin, G.B., Huang, H.C., and Collmer, A. (2007). A Pseudomonas syringae pv. tomato DC3000 mutant lacking the type III effector HopQ1-1 is able to cause disease in the model plant Nicotiana benthamiana. Plant J 51, 32-46. Yamamizo, C., Kuchimura, K., Kobayashi, A., Katou, S., Kawakita, K., Jones, J.D., Doke, N., and Yoshioka, H. (2006). Rewiring mitogen-activated protein kinase cascade by positive feedback confers potato blight resistance. Plant Physiol 140, 681-692. Yarunin, A., Panse, V.G., Petfalski, E., Dez, C., Tollervey, D., and Hurt, E.C. (2005). Functional link between ribosome formation and biogenesis of iron-sulfur proteins. EMBO J 24, 580-588. Yoo, S.D., Cho, Y.H., and Sheen, J. (2007). Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc 2, 1565-1572. Yoshioka, H., Numata, N., Nakajima, K., Katou, S., Kawakita, K., Rowland, O., Jones, J.D., and Doke, N. (2003). Nicotiana benthamiana gp91phox homologs NbrbohA and NbrbohB participate in H2O2 accumulation and resistance to Phytophthora infestans. Plant Cell 15, 706-718. Zhang, H., Zhang, D., Chen, J., Yang, Y., Huang, Z., Huang, D., Wang, X.C., and Huang, R. (2004). Tomato stress-responsive factor TSRF1 interacts with ethylene responsive element GCC box and regulates pathogen resistance to Ralstonia solanacearum. Plant Mol Biol 55, 825-834. Zhou, J., Zhang, H., Yang, Y., Zhang, Z., Hu, X., Chen, J., Wang, X.C., and Huang, R. (2008). Abscisic acid regulates TSRF1-mediated resistance to Ralstonia solanacearum by modifying the expression of GCC box-containing genes in tobacco. J Exp Bot 59, 645-652. Zhu, X., and Dinesh-Kumar, S.P. (2008). Virus-induced gene silencing as a tool to identify host genes affecting viral pathogenicity. Methods Mol Biol 451, 641-648. Zimmerman, C., Klein, K.C., Kiser, P.K., Singh, A.R., Firestein, B.L., Riba, S.C., and Lingappa, J.R. (2002). Identification of a host protein essential for assembly of immature HIV-1 capsids. Nature 415, 88-92. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/40623 | - |
| dc.description.abstract | 植物遭受到生物性逆境後會經由一系列的訊息傳遞產生防禦機制，有效對抗不同病菌。青枯病（bacterial wilt）為細菌性維管束病害，造成全球許多作物的嚴重致死萎凋病。但目前對於植物抗青枯病菌 (Ralstonia solanacearum) 的抗病機制研究相當不足，尤其茄科植物是青枯病菌主要的天然宿主，其分子抗病機制研究卻很有限。所以本研究選擇番茄（Solanum lycopersicum）抗病品系H7996為對象，探討茄科作物對抗青枯病菌的分子防禦機制。首先利用Tobacco Rattle Virus (TRV)-based vector 進行virus-induced gene silencing (VIGS)，找出最適合抗青枯病番茄品系H7996的基因靜默條件，以有效地篩選青枯病菌防禦基因。以農桿菌注射在子葉後觀察植株不同部位出現基因靜默的時間點。結果發現第五天於番茄地基部開始出現基因靜默現象，在根部是第七天開始發生，在新生葉則是在第十天開始發生。基因靜默後第十天效果可擴散到全株，故選擇此時間點作青枯病試驗的接種病菌時間點。此外，本研究亦發現利用TRV-VIGS進行多基因靜默時，將欲靜默的基因片段同時建構在一個載體的方法比利用混合農桿菌法的靜默效果佳。其次，利用上述建立之TRV-VIGS系統探討番茄青枯病的抗病訊息傳導，結果顯示防禦相關的Mitogen-activated protein kinases (MAPKs) 和水楊酸(salicylic acid)相關訊息傳遞基因皆參與番茄抗青枯病機制，這是首次在茄科作物真正瞭解水楊酸與MAPK相關基因參與抗青枯病的訊息傳導路徑。最後，針對實驗室前人選自番茄microarray分析之特定青枯病菌誘導性基因群，利用VIGS初步判定其是否參與抗病反應，結果發現其中七個基因可能參與番茄抗青枯病機制，並對其中SlCLL 和SlRLI2基因的特性進行進一步研究。這是第一篇探討番茄青枯病抗病訊息傳遞路徑的研究，也鑑認出一些新的番茄抗病防禦基因，值得未來更深入研究。 | zh_TW |
| dc.description.abstract | Bacterial wilt (BW), caused by Ralstonia solanacearum, is a devastating vascular disease of many economically important crops worldwide. However, information on plant defense mechanism to the infection of this soil-borne bacterium is limited. In this study, virus-induced gene silencing (VIGS) was employed to decipher signaling pathways involved in tomato resistance to this pathogen. Firstly, factors involved in Tobacco rattle virus (TRV)-based VIGS system were optimized for efficient gene functional study in tomato. Spatial and temporal analysis showed that gene silencing in 9-day-old tomato plant initiates 5 days post inoculation (DPI) in stembases, 7 DPI in roots and 9 DPI in young leaves. Analysis involving multi-gene silencing revealed that a chimeric construction approach is more efficient as compared to mixing multiple Agrobacterium strains carrying individual target genes. By employing the optimized system, this study further revealed silenced salicylic acid-related signaling transduction pathways and mitogen-associated protein kinase cascades play role in tomato BW-resistance. This is the first report elucidating the intertwined defense network governing resistance to bacterial wilt in tomato. Finally, previous microarray analysis led to the identification of a group genes differentially expressed specifically in H7996. Using the VIGS system, this study further confirmed a few of these genes do play a role in tomato defense to R. solanacearum. Two of these genes, SlCLL and SlRLI2, were subjected to further characterization. Together these studies enlighten molecular mechanism of tomato BW resistance and genes involved. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-14T16:53:39Z (GMT). No. of bitstreams: 1 ntu-97-R95b42023-1.pdf: 1909120 bytes, checksum: 7c50229ac765c72a0b6706ce1df7ae60 (MD5) Previous issue date: 2008 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
致謝 ii 中文摘要 iii Abstract iv Abbreviations v Contents vi Table contents viii Figure contents ix Appendix contents x 1 INTRODUCTION 1 1.1 Overview of plant defense 1 1.2 Current studies on bacterial wilt caused by Ralstonia solanacearum 2 1.3 Virus-induced gene silencing (VIGS) 3 1.4 Motivation 4 2 MATERIALS AND METHODS 5 2.1 Plant material 5 2.2 Isolation of total RNA from plant material 5 2.3 Reverse transcription polymerase chain reaction (RT-PCR) 6 2.4 Plasmid construction 8 2.5 Transformation of competent cell 9 2.6 Isolation of Plasmid DNA 10 2.7 Quantification of RNA (Quant-iTTM RNA assay Kit ,Invitrogen) 11 2.8 Nouthern bloting 12 2.9 Virus induced gene silencing (VIGS) 13 2.10 Assessment of plant response to the infection of R. solanacearum 14 2.11 Semiquantitative RT-PCR 15 2.12 Subcellular localization assay using Arabidopsis protoplast 16 2.13 Agro-transformation of Arabidopsis by floral dip 17 2.14 Bioinformatic and Statistic analysis 17 3 RESULTS 19 3.1 Spatial and temporal analysis of VIGS efficacy 19 3.2 VIGS-mediated simultaneous silencing of multiple genes 19 3.3 Silencing of genes known or predicted to involve in SA- and MAPK-related signaling pathways 20 3.4 Silencing of genes involved in SA- and MAPK-related signaling pathways reduced tomato resistance to bacterial wilt 22 3.5 Validation of the involvement of genes selected from microarray analysis in tomato resistance to BW 23 3.6 Further characterization of SlCLL and SlRLI2 24 4 DISCUSSION 26 4.1 Optimization of VIGS system 26 4.2 Tomato resistance to BW involved SA- and MAPK-related signaling transduction pathways 28 4.3 VIGS of genes selected from array 31 4.4 Functional study of SlCLL 32 4.5 Functional study of SlRLI2 33 4.6 Conclusion 34 REFERENCES 36 | |
| dc.language.iso | en | |
| dc.subject | 水楊酸 | zh_TW |
| dc.subject | 青枯病菌 | zh_TW |
| dc.subject | 番茄 | zh_TW |
| dc.subject | MAPK | zh_TW |
| dc.subject | VIGS | zh_TW |
| dc.subject | Solanum lycopersicum | en |
| dc.subject | MAPK | en |
| dc.subject | VIGS | en |
| dc.subject | SA | en |
| dc.subject | Ralstonia solanacearum | en |
| dc.title | 番茄對抗青枯病菌之
分子機制研究 | zh_TW |
| dc.title | Molecular defense mechanism of tomato against bacterial wilt | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 葉開溫,邱子珍,葉信宏,鄭石通 | |
| dc.subject.keyword | 番茄,青枯病菌,水楊酸,VIGS,MAPK, | zh_TW |
| dc.subject.keyword | Solanum lycopersicum,Ralstonia solanacearum,SA,VIGS,MAPK, | en |
| dc.relation.page | 41 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2008-07-30 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-97-1.pdf 未授權公開取用 | 1.86 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。