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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 李百祺(Pai-Chi Li) | |
dc.contributor.author | Wen-Shao Wu | en |
dc.contributor.author | 吳文卲 | zh_TW |
dc.date.accessioned | 2021-05-13T08:41:14Z | - |
dc.date.available | 2017-08-20 | |
dc.date.available | 2021-05-13T08:41:14Z | - |
dc.date.copyright | 2017-08-20 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-08-19 | |
dc.identifier.citation | [1] S. M. Weis and D. A. Cheresh, 'Tumor angiogenesis: molecular pathways and therapeutic targets', Nature Medicine, 17.11 (2011): 1359-1370.
[2] D. Fukumura and R. K. Jain, 'Tumor microvasculature and microenvironment: targets for anti-angiogenesis and normalization', Microvascular Research,74.2 (2007): 72-84. [3] K. M. Yamada and E. Cukierman, 'Modeling tissue morphogenesis and cancer in 3D', Cell, 130.4 (2007): 601-610. [4] C. Fischbach, et al., 'Cancer cell angiogenic capability is regulated by 3D culture and integrin engagement', Proceedings of the National Academy of Sciences, 106.2 (2009): 399-404. [5] Y. Zheng, et al., 'In vitro microvessels for the study of angiogenesis and thrombosis', Proceedings of the National Academy of Sciences, 109.24 (2012): 9342-9347. [6] M. Balu, et al., 'Effect of excitation wavelength on penetration depth in nonlinear optical microscopy of turbid media', Journal of Biomedical Optics, 14.1 (2009): 010508-010508. [7] J. Yao and L. V. Wang, 'Sensitivity of photoacoustic microscopy', Photoacoustics, 2.2 (2014): 87-101. [8] L. V. Wang and S. Hu, 'Photoacoustic tomography: in vivo imaging from organelles to organs', Science, 335.6075 (2012): 1458-1462. [9] L. Zeng, et al., 'Portable optical-resolution photoacoustic microscopy with a pulsed laser diode excitation', Applied Physics Letters,102.5 (2013): 053704. [10] T. Wang, et al., 'A low-cost photoacoustic microscopy system with a laser diode excitation', Biomedical Optics Express, 5.9 (2014): 3053-3058. [11] T. Buma, M. Spisar and M. O’Donnell, 'High-frequency ultrasound array element using thermoelastic expansion in an elastomeric film', Applied Physics Letters,79.4 (2001): 548-550. [12] Y. Hou, et al., 'Broadband all-optical ultrasound transducers', Applied Physics Letters, 91.7 (2007): 073507. [13] Y. Wang, et al., 'Integrated photoacoustic and fluorescence confocal microscopy', IEEE Transactions on Biomedical Engineering, 57.10 (2010): 2576-2578. [14] N. F. Schwenzer, et al., 'Non-invasive assessment and quantification of liver steatosis by ultrasound, computed tomography and magnetic resonance', Journal of Hepatology, 51.3 (2009): 433-445. [15] G. Xu, et al., 'Photoacoustic and ultrasound dual-modality imaging of human peripheral joints', Journal of Biomedical Optics, 18.1 (2013): 010502-010502. [16] S. Y. Nam, et al., 'In vivo ultrasound and photoacoustic monitoring of mesenchymal stem cells labeled with gold nanotracers', PLoS One, 7.5 (2012): e37267. [17] T. Harrison, et al., 'Combined photoacoustic and ultrasound biomicroscopy', Optics Express, 17.24 (2009): 22041-22046. [18] E. M. Strohm, M. J. Moore and M. C. Kolios, 'High resolution ultrasound and photoacoustic imaging of single cells', Photoacoustics, 4.1 (2016): 36-42. [19] S. Y. Hung, et al., 'Concurrent photoacoustic-ultrasound imaging using single-laser pulses', Journal of Biomedical Optics,20.8 (2015): 086004-086004. [20] T. Liu, et al., 'Photoacoustic generation by multiple picosecond pulse excitation', Medical Physics, 37.4 (2010): 1518-1521. [21] T. J. Allen, B. T. Cox and P. C. Beard, 'Generating photoacoustic signals using high-peak power pulsed laser diodes', Biomedical Optics 2005, International Society for Optics and Photonics, 2005. [22] Huang, C. H., et al. 'Calculation of the absorption coefficients of optical materials by measuring the transmissivities and refractive indices.' Optics and Laser Technology 34.3 (2002): 209-211. [23] D. S. Lin, et al., 'Encapsulation of capacitive micromachined ultrasonic transducers using viscoelastic polymer', Journal of Microelectromechanical Systems,19.6 (2010): 1341-1351. [24] J. T. Oh, et al., 'Three-dimensional imaging of skin melanoma in vivo by dual-wavelength photoacoustic microscopy', Journal of Biomedical Optics, 11.3 (2006): 034032-034032. [25] M. Liu, et al., 'In vivo three dimensional dual wavelength photoacoustic tomography imaging of the far red fluorescent protein E2-Crimson expressed in adult zebrafish', Biomedical Optics Express, 4.10 (2013): 1846-1855. [26] H. Dortay, et al., 'Dual-wavelength photoacoustic imaging of a photoswitchable reporter protein', SPIE BiOS., International Society for Optics and Photonics, 2016. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/4050 | - |
dc.description.abstract | 本研究致力於開發一套光聲/超音波雙模態顯微系統,以建立腫瘤血管新生三維模型之影像為目標。本研究開發之系統具有微米等級解析度與高影像深度,且不同於傳統光聲影像系統與超音波影像系統,僅使用單一雷射二極體即能產生出共模態影像。系統概念採用雷射誘發式超音波(Laser induced ultrasound),以PDMS混和物之多層膜結構取代傳統超音波探頭作為超音波激發源,能夠產生高頻、窄頻超音波訊號,藉由頻譜分離法將兩種模態影像分離與分別成像,多層膜所產生之超音波訊號頻譜可創造於12 MHz以下與光聲訊號10 dB影像對比。線與腫囊複合仿體的影像建立,驗證系統具備血管及腫瘤三維影像之潛力,透過標定金奈米粒子於觀測細胞上,近紅外光雷射二極體能夠激發光聲訊號,觀測血管內皮細胞,以利進一步建立新生血管影像。 | zh_TW |
dc.description.abstract | A dual-modality photoacoustic-ultrasound system was developed for mapping three-dimensional models of tumor angiogenesis. The system features micron scale spatial resolution in photoacoustic imaging, high resolution in ultrasound imaging and high imaging depth. With a single pulsed laser diode, the system is able to make dual-modality images by laser induced ultrasound without an additional ultrasonic pulser. A multilayer film made by PDMS and graphite powder was used to generate high frequency and narrow band ultrasound. Accordingly, photoacoustic and ultrasound signals can be separated with simple filtering and the photoacoustic image and the ultrasound image can be generated concurrently. The scheme creates 10 dB signal contrast and was tested with a fiber and cyst phantom. Gold nanorods with the absorption peak in near infrared range were used to generate the photoacoustic signal. By targeting gold nanorods on the vessels, the system has the potential to investigate neovascularization and establish 3D tumor angiogenesis models. | en |
dc.description.provenance | Made available in DSpace on 2021-05-13T08:41:14Z (GMT). No. of bitstreams: 1 ntu-105-R03945013-1.pdf: 3392398 bytes, checksum: be51dd0c601adc9810ef49210c22ee39 (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 口試委員審定書 I
謝誌 II 摘要 III Abstract IV 圖目錄 VIII 表目錄 X 第一章 緒論 1 1.1 研究動機 1 1.2 腫瘤血管新生 2 1.2 光聲顯微術 2 1.2.1 光聲效應原理 2 1.2.2 光聲顯微術 3 1.2.3 雷射二極體光聲顯微鏡 4 1.3 雷射誘發式超音波 5 1.4 光聲/超音波雙模態影像 5 1.4.1 雙模態系統文獻探討 6 1.5 基於單發雷射光聲/超音波影像系統 7 1.5.1 雷射誘發窄頻超音波 7 1.5.2 光聲/雷射誘發窄頻超音波系統架構 9 1.6 論文架構 9 第二章 雷射二極體光聲顯微系統 11 2.1 光聲訊號理論模型 11 2.2.1 雷射脈衝寬對光聲能量影響 12 2.2.2 雷射脈衝寬對光聲頻譜影響 13 2.2 高能量雷射二極體 14 2.3 雷射二極體產生誘發式超音波 16 2.3.1 雷射誘發窄頻超音波 18 2.4 雷射二極體光聲顯微影像訊雜比 18 2.5 光聲穿透深度 18 第三章 雷射誘發窄頻超音波系統 20 3.1 多層膜製作 20 3.2 薄膜產生超音波系統架構 20 3.3 窄頻超音波頻率響應 22 3.4 雷射誘發超音波訊號強度 23 3.5合成孔徑法 26 第四章 光聲/超音波顯微使用單發雷射成像系統 27 4.1 雷射二極體光聲/超音波顯微系統架設 27 4.2 頻帶分離法 29 4.3 系統影像分析 30 4.3.1 影像深度判別 30 4.3.2 超音波強度補償 31 4.4 系統光聲影像解析度量測 31 4.5 系統超音波影像解析度量測 32 4.5.1 薄膜產生超音波影像解析度 32 4.5.2 合成孔徑法對影像解析度及品質提升 33 4.5.3 薄膜產生超音波聲場分析 34 4.7 線仿體光聲/超音波雙模態影像 36 4.7.1 薄膜式架構雙模態影像 36 4.8 系統對奈米金桿光聲影像靈敏度 37 4.8.1 奈米金桿濃度與仿體製作 37 第五章 光聲/超音波雙模態系統於腫瘤細胞血管增生應用 39 5.1 三維線/腫囊仿體影像 39 5.1.1 影像掃描及掃描速度 39 5.1.2 三維影像建立 40 5.2 奈米金粒子標定血管內皮細胞影像 41 第六章 問題與討論 43 6.1 光聲/超音波雙模態系統優勢與限制 43 6.2 雷射二極體對系統所帶來之限制 43 6.3 影像系統解析度 44 6.4 仿體影像 44 6.5 影像空間解析度改善 44 6.5.1 結合聲透鏡與超音波薄膜系統 44 6.5.2 結合聲透鏡式系統分析 45 6.5.3 聲透鏡製作 46 6.5.4結合聲透鏡與薄膜之系統架構 47 6.5.5結合聲透鏡與薄膜之超音波影像解析度 47 第七章 結論與未來工作 49 7.1 結論 49 7.2 未來工作 49 7.2.1 光聲超音波雙模態系統應用 49 7.2.2 雙波長雷射二極體光聲顯微系統 51 參考文獻 53 | |
dc.language.iso | zh-TW | |
dc.title | 使用單發雷射之雙模態光聲/超音波顯微系統 | zh_TW |
dc.title | Cost-effective Design of a Photoacoustic-Ultrasound Microscope Using Single Laser Pulses | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 宋孔彬,劉建宏,沈哲州,郭柏齡 | |
dc.subject.keyword | 光聲顯微術,雙模態影像系統,雷射誘發式超音波,三維影像系統, | zh_TW |
dc.subject.keyword | photoacoustic microscopy,dual-modality imaging,laser induced ultrasound,3D imaging system, | en |
dc.relation.page | 55 | |
dc.identifier.doi | 10.6342/NTU201603419 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2016-08-21 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
顯示於系所單位: | 生醫電子與資訊學研究所 |
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