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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 陳進庭(Chin-Tin Chen) | |
dc.contributor.author | Tse-Hsien Wang | en |
dc.contributor.author | 王澤嫻 | zh_TW |
dc.date.accessioned | 2021-06-14T16:47:23Z | - |
dc.date.available | 2010-08-04 | |
dc.date.copyright | 2008-08-04 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-31 | |
dc.identifier.citation | 1. 楊采菱,國家衛生研究院電子報 第188期2007-02-07。
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/40428 | - |
dc.description.abstract | 為了改善疏水性光感物質於水溶液環境的溶解度及提升光動力效應,並且嘗
試以奈米載體增進光感物質與菌體之間的作用,本研究透過微脂體 (Liposome)或微胞 (Micelle) 包覆光感物質血紫質 (Hematoporphyrin,Hp) 後,藉由光譜及粒徑等相關分析探討Hp載體特性,並進一步比較free form Hp與載體Hp之間的光動力殺菌差異。 吸收與螢光光譜結果顯示,Hp以單體形式 (monomer form) 分布於載體中,粒徑可控制在100 nm大小,Hp奈米載體於低溫儲存至少可維持一個月的穩定。光動力殺菌成果方面,透過載體包埋的Hp劑型本身對菌體無毒性;光動力照光後Hp載體對革蘭氏陽性菌金黃色葡萄球菌、白色表皮葡萄球菌及化膿性鏈球菌之光動力殺菌效果優於free form;但對於革蘭氏陰性菌綠膿桿菌在現有培養條件下存活率則無顯著影響。 | zh_TW |
dc.description.abstract | To increase photodynamic efficacy and the interaction between photosensitizer and bacteria, we use liposome or micelle to encapsulate Hematoporphyrin. After the encapsulation, we first characterize these nanocarriers by analyzing the spectra and particle size, and then compare the bactericidal differences between free form Hp and carrier Hp. The UV-Vis and Fluorescence spectrophotometric spectra show that, Hp is dispersed in carriers as a monomeric form. The particles sizes of carriers are about 100 nm. And micellar formulations could maintain their storage stability as solution or lyophilized form at least for one month at 4℃.
The spectrophotometric spectra remained the same in one month, showing that no chemical degradation took place. Drug content of Hp-loaded Pluronic® micelles did not change in 1 month showing the high storage stability of these formulations. Carrier Hp themselves have no toxicity to bacteria without lighting. Under 50 J/cm2 lighting to Gram-positive bacteria S. aurues, S. epidermidis and S. pyogenes, nano-carriers achieve better phototoxicity than free form Hp, but no effect to Gram-negative bacteria P. aeruginosa survival. | en |
dc.description.provenance | Made available in DSpace on 2021-06-14T16:47:23Z (GMT). No. of bitstreams: 1 ntu-97-R95B47411-1.pdf: 1897205 bytes, checksum: 1a7e31e449d86392ca785687f2016601 (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 第一章、緒論 1
1.1 光動力殺菌 (Photodynamic inactivatin) 1 1.1.1 作用機制 1 1.2 血紫質 (Hematoporphyrin) 5 1.3 微脂體 (Liposome) 6 1.4 高分子微胞 (Polymeric micelle) 7 1.6本研究使用菌種介紹 9 1.6.1金黃色葡萄球菌 (Staphylococcus aureus) 及MRSA 9 1.6.2白色表皮葡萄球菌 (Staphylococcus epidermidis) 10 1.6.3化膿性鏈球菌 (Group A Streptococcus pyogenes,GAS) 11 1.6.4 綠膿桿菌 (Pseudomonas aeruginosa) 11 第二章、實驗動機與目的 12 第三章、材與方法 14 3.1 藥品 14 3.2 儀器 15 3.3 菌種來源與保存 16 3.3 實驗方法 17 3.3.1 微脂粒製備 17 3.3.2 微脂體藥物包覆率分析 18 3.3.3 微胞製備 19 3.3.4 Hp定量分析 20 3.3.5 Micelle藥物包覆率分析 22 3.3.6 Micelle穩定性分析 23 3.3.7 粒徑分析 Dynamic Light Scattering (DLS) 24 3.3.8 培養懸浮菌體 26 3.3.9 光動力殺菌 27 3.3.10 28 第四章、結果 29 [第一部分:Liposome及Micelle包埋Hp之基本性質分析] 29 4.1.1 光譜性質分析 29 4.1.2 Liposome與Micelle之特性分析 29 [第二部分:Liposome及Micelle應用於光動力殺菌之成效] 30 4.2.1 培養時間對金黃色葡萄球菌光動力殺菌之影響 30 4.2.2 Micellar Hp應用於金黃色葡萄球菌光動力殺菌之成效 30 4.2.3 劑型毒性之分析 31 4.2.4 Hp光動力應用於MRSA之成效 31 4.2.5 金黃色葡萄球菌攝入Hp後之光動力成效 31 4.2.6 Hp光動力應用於G(-)綠膿桿菌之成效 32 [第三部分] 33 4.3.1 34 4.3.2 34 4.3.3 35 4.3.4 36 4.3.5 36 4.3.6 37 [第四部分] 37 4.4.1 37 4.4.2 38 [第五部分:Micelle穩定性分析] 39 4.5.1 Micelle穩定性分析 (光譜) 39 4.5.2 Micelle穩定性分析 (粒徑) 40 第五章、討論 41 5.1 [第一部分:Liposome及Micelle包埋Hp之基本性質分析] 41 5.2 [第二部分:討論Liposome及Micelle應用於光動力殺菌之成效] 42 5.3 [第三部分] 46 5.4 [第四部分:Micellar Hp穩定性分析] 46 第六章、圖表 49 第七章、附錄 74 第八章、參考文獻 83 | |
dc.language.iso | zh-TW | |
dc.title | 以微脂體或微胞包埋血紫質之光動力殺菌探討 | zh_TW |
dc.title | Photodynamic inactivation against bacteria by Hematoporphyrin incorporated into liposome or micelle | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃 慶 璨(Ching-Tsan Huang),許瑞祥(Ruey-Shyang Hseu),鄧麗珍,蔡翠敏(Tsui-Min Tsai) | |
dc.subject.keyword | 微脂體,微胞,光動力,殺,菌,血紫質, | zh_TW |
dc.subject.keyword | Liposome,Micelle,Photodynamic inactivation,Hematoporphyrin, | en |
dc.relation.page | 90 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2008-07-31 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
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