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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 楊健志 | |
| dc.contributor.author | Yu-Peng Wang | en |
| dc.contributor.author | 王玉芃 | zh_TW |
| dc.date.accessioned | 2021-06-14T16:46:03Z | - |
| dc.date.available | 2014-08-20 | |
| dc.date.copyright | 2011-08-20 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-12 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/40376 | - |
| dc.description.abstract | 酵母菌中damage reponse protein 1 (Dap1) 及大鼠中25-Dx與阿拉伯芥中AtMAPRs同屬MAPR家族成員，此家族蛋白質具有保守性區塊cytochrome b5 like heme/steroid binding domain。本實驗室對阿拉伯芥之MAPR同源蛋白質AtMAPRs進行研究，發現重組之AtMAPRs蛋白質在胞外具有不需E3 ligase參與的「自身泛素化修飾能力」(E3-independent autoubiquitination)，序列比對結果指出AtMAPRs之cytochrome b5 like heme/steroid binding domain上可能具有類似UIM (ubiquitin-interacting motif) 的序列，其為一種泛素結合區塊UBDs (ubiquitin binding domains)，而研究指出帶有UBD之蛋白質可能會進行自身泛素化修飾。本論文利用大腸桿菌表現Dap1、25-Dx及其他參與泛素化修飾之E2，以胞外泛素化修飾實驗 (in vitro ubiquitination assay) 分析Dap1與25-Dx是否具有自身泛素化修飾之能力，發現兩者皆不具有自身泛素化修飾能力。而序列比對結果指出AtMAPR2與Dap1及25-Dx有一段較不相似之序列，此序列可能為影響Dap1及25-Dx不具自身泛素化修飾能力之主要因素。因此自身泛素化修飾能力可能為阿拉伯芥中AtMAPRs特有之能力，此推論有待進一步實驗來證實。 | zh_TW |
| dc.description.abstract | AtMAPRs in Arabidopsis are homologous to Dap1 in Saccharomyces cerevisiae and 25-Dx in Rattus norvegicus. They are members of MAPR (membrane-associated progesterone receptor) family which characterized by a conserved cytochrome b5 like heme/steroid binding domain. In previous study, it has been demonstrated that the recombinant proteins of AtMAPRs, the MAPR homologs in Arabidopsis thaliana, have E3-independent autoubiquitination activity in vitro. A putative ubiquitin-interacting motif (UIM) was detected in the cytochrome b5 like heme/steroid binding domain of AtMAPRs from a multiple sequence alignment. UIM is a member of UBDs (ubiquitin-binding domains), and the UBD-containing proteins have shown autoubiquitination activity. In this study, recombinant proteins required for the in vitro ubiquitination assay, E2 (UBC4, UBE2D2) and substrate (Dap1, 25-Dx), were produced in E. coli. In vitro ubiquitination assays indicated that Dap1 and 25-Dx did not have autoubiquitination activity. There are some differences in Dap1 and 25-Dx sequences compared with AtMAPR2. This may explain that Dap1 and 25-Dx did not have autoubiquitination activity. These findings suggested that E3-independent autoubiquitination might be an unique character of AtMAPRs in Arabidopsis. Further studies are needed to support this assumption. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-14T16:46:03Z (GMT). No. of bitstreams: 1 ntu-100-R98b47210-1.pdf: 3130995 bytes, checksum: 848f32d161b08ec3b282606c365f8c78 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 縮寫表 I
摘要 IV Abstract V 第一章 緒論 1 1.1 泛素化修飾作用 1 1.1.1 泛素 (ubiquitin) 1 1.1.2 泛素活化酶 (ubiquitin-activating enzyme, E1) 2 1.1.3 泛素銜接酶 (ubiquitin-conjugating enzyme, E2) 3 1.1.4 泛素接合酶 (ubiquitin ligase, E3) 3 1.1.5 泛素化修飾形式 4 1.1.5.1 Lys48相連聚泛素化修飾 (Lys48-linked polyubiquitination) 4 1.1.5.2 Lys63相連聚泛素化修飾 (Lys63-linked polyubiquitination) 4 1.1.5.3 單一泛素化修飾 (monoubiquitination) 4 1.1.5.4 直線型聚泛素化修飾 (linear polyubiquitination) 5 1.1.5.5 其他聚泛素化修飾形式 5 1.1.6 ubiquitin-binding domains (UBDs) 6 1.1.6.1 UBD種類 6 1.1.6.1.1 Helix domains 7 1.1.6.1.2 ZnF domains 8 1.1.6.1.3 Ubc (ubiquitin-conjugating enzyme) -related domains 10 1.1.6.2 Coupled monoubiquitination 11 1.2 MAPR (PGRMC1) 家族 12 1.2.1 MAPR家族特性 13 1.2.2 MAPR家族成員 13 1.2.2.1 Dap1 (damage response protein 1; damage-associated response protein 1) 13 1.2.2.2 25-Dx (IZA; ratp28; VEMA; mPR) 15 1.2.3 MAPR家族可能之受質 16 1.2.3.1 黃體激素 (progesterone) 16 1.2.3.2 血基質 (heme) 17 1.3 AtMAPR家族 19 1.4 研究動機與方向 20 第二章 材料與方法 22 2.1 實驗材料 22 2.1.1 載體 (Vectors) 22 2.1.2 菌種 22 2.2 實驗藥品 23 2.2.1 一般化學藥劑 23 2.2.2 酵素 23 2.2.3 培養基 24 2.3 儀器設備 25 2.4 實驗方法 27 2.4.1 DNA相關之基本操作方法 27 2.4.1.1 質體DNA小量製備 27 2.4.1.2 DNA限制酶切割反應 (Restriction enzyme digestion) 28 2.4.1.3 DNA洋菜瓊脂糖膠體電泳 (Agarose gel electrophoresis) 28 2.4.1.4 DNA之定量 28 2.4.1.5 質體轉型－熱衝擊轉型法 (heat-shock transformation) 29 2.4.1.6 Gateway cloning 29 2.4.2 RNA相關之基本操作方法 31 2.4.2.1 Total RNA之抽取 31 2.4.2.2 RNA之定量 32 2.4.2.3 甲醛洋菜瓊脂膠體電泳 (formaldehyde-agarose gel electrophoresis)32 2.4.2.4 RT –PCR (reverse transcriptase polymerase chain reaction) 33 2.4.3目標基因之選殖及載體建構 34 2.4.3.1 Damage resistance protein 1 (Dap1) 保存載體與表現載體之建構 34 2.4.3.2 Ubiquitin-conjugating enzyme E2 4 (UBC4) 保存載體與表現載體之建構 35 2.4.3.3 25-DX保存載體與表現載體之建構 35 2.4.3.4 Ubiquitin-conjugating enzyme E2 D2 (UBE2D2) 保存載體與表現載體之建構 36 2.4.4 蛋白質相關之基本操作方法 37 2.4.4.1 蛋白質電泳分析 37 2.4.4.1.1 SDS膠體電泳 (SDS-PAGE) 37 2.4.4.1.2 梯度SDS膠體電泳 (gradient SDS-PAGE) 39 2.4.4.2 Coomassie Brilliant Blue R-250 (CBR) 膠片染色法 42 2.4.4.3 膠片乾燥法 43 2.4.4.4 蛋白質轉印 43 2.4.4.5 免疫呈色 44 2.4.4.6 Bradford蛋白質定量法 45 2.4.4.7 各目標基因重組蛋白質表現與純化 45 2.4.4.7.1 His6-Dap、His6-25-DX及His10-AtMAPR2之表現與純化 45 2.4.4.7.2 GST-UBC4、GST-UBE2D2及GST-AtUBC8之表現與純化 47 2.4.4.8 胞外泛素化修飾分析 (In vitro ubiquitination assay) 48 2.4.4.9 蛋白質質譜分析 49 2.4.4.9.1 In-gel digestion 50 2.4.4.9.2 MALDI-Q-TOF (matrix-assisted laser desorption and ionization- quadrupore-time of flight mass spectrometer) 51 2.4.4.9.3 LC/MS-MS (liquid chromatography-tandem mass spectrometry) 51 第三章 結果與討論 53 3.1 胞外泛素化修飾分析 (In vitro ubiquitination assay) 53 3.1.1 胞外泛素化修飾分析之材料 53 3.1.2 表現質體之建構與確認 54 3.1.2.1 attB-Dap1與attB-UBC4基因序列選殖 54 3.1.2.2 pDONR221-Dap1與pDONR221-UBC4保存質體建構及確認 54 3.1.2.3 pDEST17-Dap1與pDEST15-UBC4表現質體建構及確認 55 3.1.3 重組蛋白質表現與純化分析 55 3.1.3.1 His6-Dap1重組蛋白質表現與純化分析 55 3.1.3.2 GST-UBC4重組蛋白質表現與純化分析 56 3.1.3.3 GST-AtUBC8重組蛋白質表現與純化分析 57 3.1.3.4 His10-AtMAPR2重組蛋白質表現與純化分析 57 3.1.3.5 His6-25-Dx重組蛋白質表現與純化分析 58 3.1.3.6 GST-UBE2D2重組蛋白質表現與純化分析 59 3.1.4 His6-Dap1可能不具有胞外自身泛素化活性 59 3.1.4.1 以GST-UBC4作為泛素化修飾分析之E2 59 3.1.4.2 以GST-AtUBC8作為泛素化修飾分析之E2 61 3.1.5 His6-25-Dx可能不具有胞外自身泛素化活性 62 3.1.6 蛋白質身分鑑定與泛素化修飾色帶分析 63 3.2 Dap1、25-Dx與AtMAPR2蛋白質序列比對分析 64 3.3 結論 65 第四章 未來展望 67 4.1 探討原生物種中表現之Dap1與25-Dx是否具有胞外自身泛素化修飾活性 67 4.2 探討細胞內過度表現之Dap1與25-Dx是否具有自身泛素化修飾活性 67 4.3 尋找AtMAPR2上可能之UBD位置以確認Dap1與25-Dx序列上是否具有UBD 68 4.4 探討其他物種之MAPR是否具有UBD 68 參考文獻 69 圖與表 81 附錄 103 口試問答集 115 | |
| dc.language.iso | zh-TW | |
| dc.subject | 內噬作用 | zh_TW |
| dc.subject | 泛素化修飾 | zh_TW |
| dc.subject | 自身泛素化修飾 | zh_TW |
| dc.subject | 泛素結合區塊 | zh_TW |
| dc.subject | 黃體激素 | zh_TW |
| dc.subject | autoubiquitination | en |
| dc.subject | UBD | en |
| dc.subject | MAPR | en |
| dc.subject | Dap1 | en |
| dc.subject | 25-Dx | en |
| dc.title | 探討Dap1與25-Dx是否具有自身泛素化修飾能力之研究 | zh_TW |
| dc.title | Studies on the Potential Autoubiquitination of Dap1 and 25-Dx | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蘇仲卿,陳佩燁,常怡雍,張俊哲 | |
| dc.subject.keyword | 泛素化修飾,自身泛素化修飾,泛素結合區塊,黃體激素,內噬作用, | zh_TW |
| dc.subject.keyword | Dap1,25-Dx,autoubiquitination,MAPR,UBD, | en |
| dc.relation.page | 117 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-12 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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