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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳凱儀 | |
| dc.contributor.author | Yu-Ya Liang | en |
| dc.contributor.author | 梁祐雅 | zh_TW |
| dc.date.accessioned | 2021-05-13T08:40:53Z | - |
| dc.date.available | 2017-02-16 | |
| dc.date.available | 2021-05-13T08:40:53Z | - |
| dc.date.copyright | 2016-02-16 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-01-25 | |
| dc.identifier.citation | 鄭安秀. (2001) 番茄與馬鈴薯晚疫病. 台南區農業專訊第37期：13-16.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/4034 | - |
| dc.description.abstract | 本研究室先前的數量性狀基因座遺傳定位研究指出，野生番茄L3708 (Solanum pimpinellifolium L.) 除了在第九對染色體具有晚疫病抗病基因Ph-3以外，於第二對染色體上亦具有另一個抗病基因qPh2.1。延續先前研究結果，本研究使用與先前研究相同的野生番茄L3708與栽培番茄T3224雜交族群，以其衍生之自交F3:4子代為接種材料進行研究。並設定兩個研究目標：(1) Ph-3抗病基因的精確定位；(2) 新抗病基因qPh2.1對於晚疫病抗病能力的確認，並評估qPh2.1基因座的遺傳特性。
研究結果指出，qPh2.1確實能賦予番茄對晚疫病之抗性，其抗病對偶基因型相對於感病對偶基因型為隱性。根據番茄全基因體組參照序列SL 2.5，本研究將Ph-3抗病基因定位於番茄第九對染色體鹼基序列71,356,855 bp至71,501,998 bp區間；qPh2.1則被定位於番茄第二對染色體鹼基序列 28,042,265 bp的前方染色體區域。 | zh_TW |
| dc.description.abstract | Our previous QTL mapping study showed that the wild tomato accession L3708 (Solanum pimpinellifolium L.) harbors a new late blight resistant gene qPh2.1 on chromosome 2, in addition to the Ph-3 gene on chromosome 9. The aims of present study were as following: (1) Ph-3 fine mapping; (2) genetic characterization of the resistance of qPh2.1. In the current study, the qPh2.1 resistance to late blight disease was validated and characterized using isogenic lines derived from the previous F2:3 genetic mapping population which was generated using L3708 as the resistant parent. Our results indicated that the resistant allele of qPh2.1 was recessive to the susceptible allele. The qPh-3 gene was delimited in the region between 71,356,855 bp to 71,501,998 bp; and the qPh2.1 gene was delimited in the chromosomal region ahead of 28,042,265 bp on chromosome 2, according to the tomato SL 2.5 genomic reference sequences. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-13T08:40:53Z (GMT). No. of bitstreams: 1 ntu-105-R02621109-1.pdf: 1833790 bytes, checksum: c17bdc9159561b193617acc1d5009490 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 致謝 i
中文摘要 ii Abstract iii 目錄 iv 圖目錄 v 表目錄 vi 第一章 前言 1 第一節 晚疫病簡介 1 第二節 晚疫病抗病基因之研究 2 第三節 本研究室先前的相關研究 4 第二章 研究目的與研究策略 6 第三章 材料與方法 7 第一節 試驗材料 7 第二節 病原菌材料與病徵評估 12 第三節 DNA抽取 14 第四節 分子標記設計與基因型定型 (genotyping) 15 第四章 結果與討論 19 第一節 病原菌生理小種之界定 19 第二節 Ph-3之精確定位 (fine mapping) 19 第三節 qPh2.1抗病能力 21 第四節 qPh2.1為隱性抗病基因 22 第五節 qPh2.1之精確定位 24 第六節 番茄第二對染色體重組率分析 25 第五章 結論 26 引用文獻 27 附錄 40 | |
| dc.language.iso | zh-TW | |
| dc.subject | qPh2.1 | zh_TW |
| dc.subject | 番茄 | zh_TW |
| dc.subject | 晚疫病 | zh_TW |
| dc.subject | 抗病基因 | zh_TW |
| dc.subject | Ph-3 | zh_TW |
| dc.subject | Tomato | en |
| dc.subject | qPh2.1 | en |
| dc.subject | Ph-3 | en |
| dc.subject | resistant gene | en |
| dc.subject | late blight | en |
| dc.title | 番茄晚疫病抗病基因座qPh2.1遺傳特性探討 | zh_TW |
| dc.title | Genetic characterization of the late blight resistant locus qPh2.1 in tomato | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 劉瑞芬,王肇芬,胡凱康 | |
| dc.subject.keyword | 番茄,晚疫病,抗病基因,Ph-3,qPh2.1, | zh_TW |
| dc.subject.keyword | Tomato,late blight,resistant gene,Ph-3,qPh2.1, | en |
| dc.relation.page | 64 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2016-01-25 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農藝學研究所 | zh_TW |
| 顯示於系所單位： | 農藝學系 | |
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