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DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 宋孔彬 | |
dc.contributor.author | Lian-Jing Liou | en |
dc.contributor.author | 留連晉 | zh_TW |
dc.date.accessioned | 2021-06-14T16:43:42Z | - |
dc.date.available | 2008-08-04 | |
dc.date.copyright | 2008-08-04 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-31 | |
dc.identifier.citation | [1] 胡品文,”應用表面電漿感測器於生物分子作用之動力學及構型分析”,國立成功大學醫學工程研究所博士論文,民國九十五年九月.
[2] Homola ,“Surface plasmon resonance sensors: review”, Sensors and Actuators B 54 (1999) 3–15. [3] 吳民耀、劉威志,表面電漿子理論與模擬,物理雙月刊(廿八卷二期)2006 年4月 [4] H.Raeter ,”Surface Plasmons on Smooth and Rough Surface and on Grating”, Springer Tracts in Modern Physics, vol. III,Springer, Berlin–Heidelberg, 1988, 136 pp. [5] A. Sommerfeld, Ann. der Physik, 28, 665--736 (1909). [6] A. Otto, Excitation of surface plasma waves in silver by the method of frustrated total reflection, Z. Physik 216 (1968) 398–410. [7] E. Kretschmann, H. Raether, Radiative decay of non-radiative surface plasmons excited by light, Z. Naturforsch. 23A (1968) 2135–2136 [8] Homola ,“Present and future of surface plasmon resonance biosensors”, Anal Bioanal Chem (2003) 377 : 528–539 [9] Homola ,“Surface plasmon resonance sensors based on diffraction gratings and prism couplers: sensitivity comparison”, Sensors and Actuators B 54 (1999) 16–24 [10] Gupta,“Tuning and sensitivity enhancement of surface plasmon resonance sensor”, Sensors and Actuators B 122 (2007) 381–388. [11] Hizuru Nakajima ,“A palm-sized surface plasmon resonance sensor with microchip flow cell”, Talanta 70 (2006) 419–425. [12] Gaurav Gupta,” Use of a low refractive index prism in surface plasmon resonance biosensing ”, Sensors and Actuators B 130 (2008) 689–695. [13] B.E.A. Saleh , M.C Teich , “FUNDAMENTALS OF PHOTONICS” ,P.253 [14] Pharmacia Biosensor AB Product Catalog, 1995. [15] E. Stenberg, B. Persson, H. Roos, C. Urbaniczky, Quantitative determination of surface concentration of proteins with surface plasmon resonance using radiolabeled proteins, J. Colloid. Interf. Sci. 143 (1991) 513–526. [16] Jihua Guo , ” Small-angle measurement based on surface-plasmon resonance and the use of magneto-optical modulation”, APPLIED OPTICS , Vol. 38, No. 31 ,1 November 1999 [17] J. Rooney, “Designing a curved surface SPR device”, Sensors and Actuators B 114 (2006) 804–811 [18] B.E.A. Saleh , M.C Teich , “FUNDAMENTALS OF PHOTONICS” , P.205 [19] Homola ,“On the sensitivity of surface piasmon resonance sensors with spectral interrogation”, Sensors and Actuators B 41 (1997) 207-211 [20] Assistant catalog, http://www.hecht-assistent.de [21] CVI technical notes. http://www.cvilaser.com/Common/PDFs/Dispersion_Equations.pdf [22] Homola ,“Novel polarization control scheme for spectral surface plasmon resonance sensors”, Sensors and Actuators B 54 (1999) 3–15. [23] THORLABS catalog, http://www.thorlabs.com/catalogPages/772.pdf [24] http://asemblon.com/node/60/print [25] PIERCE catalog . http://www.piercenet.com/Products/Browse.cfm?fldID=02040114&WT.mc_id=keyname [26] 蘇明啟,”微奈米結構設計於SPR生醫感測元件的應用”, 民國九十四年七月. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/40267 | - |
dc.description.abstract | 表面電漿共振技術近年來被大量應用在發展生物分子感測器上,這是一種利用光學的方式,便可達到非標記、高敏感度、少量樣本、即時的檢驗方法,利用生物分子免疫分析的特殊選擇性,可以在複雜的混合物中偵測到相當低濃度的特定分子。本研究著重於光學系統架設與應用,先從理論與模擬開始探討,利用稜鏡耦合的方式激發表面電漿波,進而建立一組可靠的生物分子感測系統,使用不同濃度的蔗糖水溶液對系統進行測試其靈敏度147.34◦/RIU與解析度(每個像素:3.5×10-5RIU),最後應用於微奈米流道晶片,利用微奈米流道尺寸上的特性,提高分子偵測的濃度範圍,從蛋白質免疫分析實驗可知SPR角度的偏移在普通金膜實驗上為0.1°而在微流道晶片中增加至1.1°。 | zh_TW |
dc.description.abstract | Surface plasmon resonance (SPR) is a kind of optical detecting method and popular using in biosensor recent years. Compared with other technologies, SPR biosensor has several advantages such as real-time monitoring, label-free detection and small sample volume .Compare with the immunoassay of biomolecule , SPR sensing could provide high specificity and sensitivity in detect analytes in complex biological media.
In this paper, we focus on SPR optical system’s building and application of biosensing. We discuss SPR from theory to simulation and build a SPR biosensing system by using prism coupler mode to excite Surface plasmon wave. Then we use different concentration of sucrose solution to test our SPR system and provide a good system sensitivity(147.34◦/RIU) and resolution(each CCD pixel : 3.5×10-5RIU). At last, we combine SPR system with micro/nano-fluidic chip and use the advantage of the micro channel size to enhance the detection range of biomolecule immunoassay. From the result of protein immunoassay experiment, we find out the SPR shift angle is 0.1° in bulk gold chip and it raise increase to 1.1° in micro/nano- fluidic chip. | en |
dc.description.provenance | Made available in DSpace on 2021-06-14T16:43:42Z (GMT). No. of bitstreams: 1 ntu-97-R95921114-1.pdf: 2313592 bytes, checksum: a59730b832cc770ed6bf9338cd1a998a (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 致謝.............................................II
中文摘要....................................III ABSTRACT.....................................IV 目錄........................................V 圖目錄....................................VII 表目錄......................................XI 第一章 導論...............................1 1.1 研究背景.............................1 1.2 研究動機與目的......................4 1.3 本文結構............................5 第二章 實驗理論與模擬....................6 2.1表面電漿理論.........................6 2.2 相關文獻回顧........................12 2.3稜鏡耦合組態之原理...................16 2.4 SPR角度計算與模擬...................19 第三章 實驗材料與方法....................23 3.1 實驗系統架設........................24 3.2金屬膜與稜鏡的選擇與製備.............27 3.2.1 稜鏡的選擇.......................27 3.2.2 金屬膜的選擇與製備...............29 3.3 待測樣本的製備......................34 3.3.1 蔗糖水溶液及折射率分析...........34 3.3.2 待測蛋白質的分子固化流程.........35 3.4 SPR訊號量測方式與分析方法...........40 3.5 微奈米流道晶片......................43 第四章 實驗結果與討論....................45 4.1普通金膜晶片之訊號分析結果............45 4.1.1 蔗糖水溶液之訊號分析..............45 4.1.2 蛋白質樣本之訊號分析..............49 4.2 微流道晶片之訊號分析結果.............54 4.2.1 蔗糖水溶液之訊號分析...............55 4.2.2 蛋白質樣本之訊號分析..............58 第五章 結論與未來展望.....................59 參考文獻...................................61 | |
dc.language.iso | zh-TW | |
dc.title | 表面電漿共振感測系統應用於微奈米流體晶片之蛋白質檢測 | zh_TW |
dc.title | Application of Surface Plasmon Resonance sensing for protein immunoassay in a micro/nano-fluidic chip | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林啟萬,林致廷 | |
dc.subject.keyword | 表面電漿共振,生物分子感測器,免疫分析,稜鏡耦合,微奈米流道晶片, | zh_TW |
dc.subject.keyword | Surface plasmon resonance,biosensor,immunoassay,prism coupler,micro/nano- fluidic chip, | en |
dc.relation.page | 62 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2008-08-01 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 電機工程學研究所 | zh_TW |
顯示於系所單位: | 電機工程學系 |
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