請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/40257
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 賴爾?(Erh-Min Lai) | |
dc.contributor.author | Yi-Chun Chen | en |
dc.contributor.author | 陳怡君 | zh_TW |
dc.date.accessioned | 2021-06-14T16:43:31Z | - |
dc.date.available | 2011-08-04 | |
dc.date.copyright | 2008-08-04 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-08-01 | |
dc.identifier.citation | Alt-Moerbe, J., P. Neddermann, J. von Lintig, , & J. Schroder, (1988) Temperature-sensitive step in Ti plasmid vir-region induction and correlation with cytokinin secretion by Agrobacteria. Mol Gen Genet 213:1–8.
Aly, K. A. & C. Baron, (2007) The VirB5 protein localizes to the T-pilus tips in Agrobacterium tumefaciens. Microbiology 153: 3766-3775. Anand, A., S. R. Uppalapati, C. M. Ryu, S. N. Allen, L. Kang, Y. Tang & K. S. Mysore,(2008) Salicylic acid and systemic acquired resistance play a role in attenuating crown gall disease caused by Agrobacterium tumefaciens. Plant Physiol 146: 703-715. Anderson, L. B., A. V. Hertzel & A. Das, (1996) Agrobacterium tumefaciens VirB7 and VirB9 form a disulfide-linked protein complex. Proc Natl Acad Sci U S A 93:8889-8894. Aoyama, T., M. Takanami & A. Oka, (1989) Signal structure for transcriptional activation in the upstream regions of virulence genes on the hairy-root-inducing plasmid A4. Nucleic Acids Res 17: 8711-8725. Atmakuri, K., E. Cascales & P. J. Christie, (2004) Energetic components VirD4, VirB11 and VirB4 mediate early DNA transfer reactions required for bacterial type IV secretion. Mol Microbiol 54: 1199-1211. Backert, S. & T. F. Meyer, (2006) Type IV secretion systems and their effectors in bacterial pathogenesis. Curr Opin Microbiol 9:207-217. Ballas, N. & V. Citovsky, (1997) Nuclear localization signal binding protein from Arabidopsis mediates nuclear import of Agrobacterium VirD2 protein. Proc Natl Acad Sci U S A 94: 10723-10728. Baron, C., M. Llosa, S. Zhou & P. C. Zambryski, (1997) VirB1, a component of the T-complex transfer machinery of Agrobacterium tumefaciens, is processed to a C-terminal secreted product, VirB1. J Bacteriol 179: 1203-1210. Beijersbergen, A., A. D. Dulk-Ras, R. A. Schilperoort & P. J. Hooykaas, (1992)Conjugative transfer by the virulence system of Agrobacterium tumefaciens. Science 256: 1324-1327. Berger, B. R. & P. J. Christie, (1994) Genetic complementation analysis of the Agrobacterium tumefaciens virB operon: virB2 through virB11 are essential virulence genes. J Bacteriol 176: 3646-3660. Bevan, M. W. & M. D. Chilton, (1982) T-DNA of the Agrobacterium Ti and Ri plasmids. Annu Rev Genet 16: 357-384. Bolton, G. W., E. W. Nester & M. P. Gordon, (1986) Plant phenolic compounds induce expression of the Agrobacterium tumefaciens loci needed for virulence. Science 232: 983-985. Boone, D. R., R. W. Castenholz, & G. M. Garrity, (2001) Bergey's manual of systematic bacteriology / George M. Garrity, editor-in-chief, 2nd ed. Springer, New York. Braun, A.C. (1947) Thermal studies on the factors responsible for tumor initiation in crown-gall. Am J Bot 34:234–240. Brencic, A. & S. C. Winans, (2005) Detection of and response to signals involved in host-microbe interactions by plant-associated bacteria. Microbiol Mol Biol Rev 69: 155-194. Burr, T. J., C. Bazzi, S. Sule, & L. Otten, (1998) Crown gall of grape: biology of Agrobacterium vitis and the development of disease control strategies. Plant Dis 82:1288-1297. Cangelosi, G. A., R. G. Ankenbauer & E. W. Nester, (1990) Sugars induce the Agrobacterium virulence genes through a periplasmic binding protein and a transmembrane signal protein. Proc Natl Acad Sci U S A 87: 6708-6712. Cangelosi, G. A., L. Hung, V. Puvanesarajah, G. Stacey, D. A. Ozga, J. A. Leigh & E.W. Nester, (1987) Common loci for Agrobacterium tumefaciens and Rhizobium meliloti exopolysaccharide synthesis and their roles in plant interactions. J Bacteriol 169: 2086-2091. Cangelosi, G. A., G. Martinetti, J. A. Leigh, C. C. Lee, C. Theines & E. W. Nester,(1989) Role for [corrected] Agrobacterium tumefaciens ChvA protein in export of beta-1,2-glucan. J Bacteriol 171: 1609-1615. Cascales, E. & P. J. Christie, (2003) The versatile bacterial type IV secretion systems. Nat Rev Microbiol 1: 137-49. Cascales, E. & P. J. Christie, (2004) Definition of a bacterial type IV secretion pathway for a DNA substrate. Science 304: 1170-1173. Cavara, F. (1897) Tubercolosi della vite. Intorno alla e ziologia de alcune malattie di piante coltivate. Stazoni Sperimentale Agrarie Italiane 30:483–487. Chilton, M. D., M. H. Drummond, D. J. Merio, D. Sciaky, A. L. Montoya, M. P. Gordon & E. W. Nester, (1977) Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell 11: 263-271. Cho, H. & S. C. Winans, (2005) VirA and VirG activate the Ti plasmid repABC operon, elevating plasmid copy number in response to wound-released chemical signals. Proc Natl Acad Sci U S A 102: 14843-14848. Christie, P. J., J. E. Ward, Jr., M. P. Gordon & E. W. Nester, (1989) A gene required for transfer of T-DNA to plants encodes an ATPase with autophosphorylating activity. Proc Natl Acad Sci U S A 86: 9677-9681. Christie, P. J., (1997) Agrobacterium tumefaciens T- complex transport apparatus: a paradigm for a new family of multifunctional transporters in eubacteria. J Bacteriol 179: 3085-3094. Christie, P. J., (2004) Type IV secretion: the Agrobacterium VirB/D4 and related conjugation systems. Biochim Biophys Acta 1694: 219-234. Christie, P. J., K. Atmakuri, V. Krishnamoorthy, S. Jakubowski & E. Cascales, (2005) Biogenesis, architecture, and function of bacterial type IV secretion systems. Annu Rev Microbiol 59: 451-485. Citovsky, V., A. Kapelnikov, S. Oliel, N. Zakai, M. R. Rojas, R. L. Gilbertson, T. Tzfira & A. Loyter, (2004) Protein interactions involved in nuclear import of the Agrobacterium VirE2 protein in vivo and in vitro. J Biol Chem 279: 29528-29533. Crane, Y. M. & S. B. Gelvin, (2007) RNAi-mediated gene silencing reveals involvement of Arabidopsis chromatin-related genes in Agrobacterium-mediated root transformation. Proc Natl Acad Sci U S A 104: 15156-15161. Dafny-Yelin, M., A. Levy & T. Tzfira, (2008) The ongoing saga of Agrobacterium-host interactions. Trends Plant Sci 13: 102-105. Dang, T. A. & P. J. Christie, (1997) The VirB4 ATPase of Agrobacterium tumefaciens is a cytoplasmic membrane protein exposed at the periplasmic surface. J Bacteriol 179: 453-462. Escobar, M. A. & A. M. Dandekar, (2003) Agrobacterium tumefaciens as an agent of disease. Trends Plant Sci 8: 380-386. Fabre, E. & F. Dunal (1853) Observations sur les maladies regantes de la vigne. Bull. Soc. Cent. Agric. Dep. Herault 40:46. Fullner, K. J. & E. W. Nester, (1996) Temperature affects the T-DNA transfer machinery of Agrobacterium tumefaciens. J Bacteriol 178: 1498-1504. Gelvin, S. B., (2000) Agrobacterium and plant genes involved in T-DNA transfer and integration. Annu Rev Plant Physiol Plant Mol Biol 51: 223-256. Gelvin, S. B., (2003) Agrobacterium-mediated plant transformation: the biology behind the 'gene- jockeying' tool. Microbiol Mol Biol Rev 67: 16-37. Gelvin, S. B., (2006) Agrobacterium virulence gene induction. Methods Mol Biol 343: 77-84. Goodner, B., G. Hinkle, S.Gattung, N. Miller, M. Blanchard, B.Qurollo, B. S. Goldman, Y. Cao, M. Askenazi, C. Halling, L. Mullin, K. Houmiel, J. Gordon, M. Vaudin, O. Iartchouk, A. Epp, F. Liu, C. Wollam, M.Allinger, D. Doughty, C. Scott, C. Lappas, B. Markelz, C. Flanagan, C. Crowell, J. Gurson, C.Lomo, C. Sear, G. Strub, C. Cielo, & S. Slater, (2001) Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58. Science 294: 2323-2328. Guyon, P., M. D. Chilton, P. Annik, & T. Jacques, (1980) Agropine in 'null-type' crown gall tumors: Evidence for generality of the opine concept. Proc Natl Acad Sci U S A 77: 2693-2697. Hamilton, R. H. & M. Z. Fall, (1971) The loss of tumor- initiating ability in Agrobacterium tumefaciens by incubation at high temperature. Experientia 27: 229-230. Hapfelmeier, S., N. Domke, P. C. Zambryski & C. Baron, (2000) VirB6 is required for stabilization of VirB5 and VirB3 and formation of VirB7 homodimers in Agrobacterium tumefaciens. J Bacteriol 182: 4505- 4511. Hooykaas, P. J., M. Hofker, H. den Dulk-Ras & R. A. Schilperoort, (1984) A comparison of virulence determinants in an octopine Ti plasmid, a nopaline Ti plasmid, and an Ri plasmid by complementation analysis of Agrobacterium tumefaciens mutants. Plasmid 11: 195-205. Howard EA, Zupan JR, Citovsky V, Zambryski PC (1992) The VirD2 protein of Agrobacterium tumefaciens contains a C-terminal bipartite nuclear localization signal: implications for nuclear uptake of DNA in plant cells.Cell 68: 109–118. Hwang, H. H. & S. B. Gelvin, (2004) Plant proteins that interact with VirB2, the Agrobacterium tumefaciens pilin protein, mediate plant transformation. Plant Cell 16: 3148-3167. Jakubowski, S. J., E. Cascales, V. Krishnamoorthy & P. J. Christie, (2005) Agrobacterium tumefaciens VirB9, an outer-membrane-associated component of a type IV secretion system, regulates substrate selection and T-pilus biogenesis.J Bacteriol 187: 3486-3495. Jayaswal R.K., K.Veluthambi, S.B.Gelvin & J.L.Slightom, (1987) Doublestranded cleavage of T-DNA and generation of single-stranded T-DNA molecules in Escherichia coli by a virD-encoded border-specific endonuclease from Agrobacterium tumefaciens. J Bacteriol 169: 5035–5045. Jin, S., T. Roitsch, R. G. Ankenbauer, M. P. Gordon & E. W. Nester, (1990a) The VirA protein of Agrobacterium tumefaciens is autophosphorylated and is essential for vir gene regulation. J Bacteriol 172: 525-530. Jin, S. G., R. K. Prusti, T. Roitsch, R. G. Ankenbauer & E. W. Nester, (1990b)Phosphorylation of the VirG protein of Agrobacterium tumefaciens by the autophosphorylated VirA protein: essential role in biological activity of VirG. J Bacteriol 172: 4945- 4950. Jin, S. G., T. Roitsch, P. J. Christie & E. W. Nester, (1990c) The regulatory VirG protein specifically binds to a cis-acting regulatory sequence involved in transcriptional activation of Agrobacterium tumefaciens virulence genes. J Bacteriol 172: 531-537. John, M. C. & R. M. Amasino, (1988) Expression of an Agrobacterium Ti plasmid gene involved in cytokinin biosynthesis is regulated by virulence loci and induced by plant phenolic compounds. J Bacteriol 170: 790-795. Jones, A. L., K. Shirasu & C. I. Kado, (1994) The product of the virB4 gene of Agrobacterium tumefaciens promotes accumulation of VirB3 protein. J Bacteriol 176: 5255-5261. Judd, P. K., R. B. Kumar & A. Das, (2005) The type IV secretion apparatus protein VirB6 of Agrobacterium tumefaciens localizes to a cell pole. Mol Microbiol 55:115-124. Kado, C. I. & M. G. Heskett, (1970) Selective media for isolation of Agrobacterium,Corynebacterium, Erwinia, Pseudomonas, and Xanthomonas. Phytopathology 60: 969-976. Kao, J. C., K. L. Perry & C. I. Kado, (1982) Indoleacetic acid complementation and its relation to host range specifying genes on the Ti plasmid of Agrobacterium tumefaciens. Mol Gen Genet 188: 425-432. Karunakaran, R., T. H. Mauchline, A. H. Hosie & P. S. Poole, (2005) A family of promoter probe vectors incorporating autofluorescent and chromogenic reporter proteins for studying gene expression in Gram-negative bacteria. Microbiology 151: 3249-3256. Keane, P. J., A. Kerr, & P. B. New, (1970) Crown gall of stone fruit: Identification and nomenclature of Agrobacterium isolates. Aust J Biol Sci 23:585-590. Kerr, A., P. Manigault & J. Tempe, (1977) Transfer of virulence in vivo and in vitro in Agrobacterium. Nature 265: 560-561. Klee, H., A. Montoya, F. Horodyski, C. Lichtenstein, D. Garfinkel, S. Fuller, C. Flores,J. Peschon, E. Nester & M. Gordon, (1984) Nucleotide sequence of the tms genes of the pTiA6NC octopine Ti plasmid: two gene products involved in plant tumorigenesis. Proc Natl Acad Sci U S A 81: 1728-1732. Klee, H. J., M. P. Gordon & E. W. Nester, (1982) Complementation analysis of Agrobacterium tumefaciens Ti plasmid mutations affecting oncogenicity. J Bacteriol 150: 327-331. Klee, H. J., F. F. White, V. N. Iyer, M. P. Gordon & E. W. Nester, (1983) Mutational analysis of the virulence region of an Agrobacterium tumefaciens Ti plasmid. J Bacteriol 153: 878-883. Krall, L., U. Wiedemann, G. Unsin, S. Weiss, N. Domke & C. Baron, (2002) Detergent extraction identifies different VirB protein subassemblies of the type IV secretion machinery in the membranes of Agrobacterium tumefaciens. Proc Natl Acad Sci U S A 99: 11405-11410. Kumar, R. B. & A. Das, (2001) Functional analysis of the Agrobacterium tumefaciens T-DNA transport pore protein VirB8. J Bacteriol 183: 3636-3641. Labes, M., A. Puhler & R. Simon, (1990) A new family of RSF1010-derived expression and lac-fusion broad-host- range vectors for gram-negative bacteria. Gene 89: 37-46. Lai, E. M. & C. I. Kado, (1998) Processed VirB2 is the major subunit of the promiscuous pilus of Agrobacterium tumefaciens. J Bacteriol 180: 2711-2717. Lai, E. M., H. W. Shih, S. R. Wen, M. W. Cheng, H. H. Hwang & S. H. Chiu, (2006)Proteomic analysis of Agrobacterium tumefaciens response to the vir gene inducer acetosyringone. Proteomics 6: 4130-4136. Li, L., Y. Jia, Q. Hou, T. C. Charles, E. W. Nester & S. Q. Pan, (2002) A global pH sensor: Agrobacterium sensor protein ChvG regulates acid-inducible genes on its two chromosomes and Ti plasmid. Proc Natl Acad Sci U S A 99:12369-12374. Lichtenstein, C., H. Klee, A. Montoya, D. Garfinkel, S. Fuller, C. Flores, E. Nester & M. Gordon, (1984) Nucleotide sequence and transcript mapping of the tmr gene of the pTiA6NC octopine Ti-plasmid: a bacterial gene involved in plant tumorigenesis. J Mol Appl Genet 2: 354-362. Liu, C. N., X. Q. Li & S. B. Gelvin, (1992) Multiple copies of virG enhance the transient transformation of celery, carrot and rice tissues by Agrobacterium tumefaciens. Plant Mol Biol 20: 1071-1087. Llosa, M., J. Zupan, C. Baron & P. Zambryski, (2000) The N- and C-terminal portions of the Agrobacterium VirB1 protein independently enhance tumorigenesis. J Bacteriol 182: 3437-3445. Matthysse, A. G., K. V. Holmes & R. H. Gurlitz, (1981) Elaboration of cellulose fibrils by Agrobacterium tumefaciens during attachment to carrot cells. J Bacteriol 145:583-595. Merritt, P. M., T. Danhorn & C. Fuqua, (2007) Motility and chemotaxis in Agrobacterium tumefaciens surface attachment and biofilm formation. J Bacteriol 189: 8005-8014. Mysore, K. S., J. Nam & S. B. Gelvin, (2000) An Arabidopsis histone H2A mutant is deficient in Agrobacterium T-DNA integration. Proc Natl Acad Sci U S A 97:948-953. Okker, R. J., H. Spaink, J. Hille, T. A. van Brussel, B. Lugtenberg & R. A. Schilperoort,(1984) Plant- inducible virulence promoter of the Agrobacterium tumefaciens Ti plasmid. Nature 312: 564-566. Pazour, G. J. & A. Das, (1990) Characterization of the VirG binding site of Agrobacterium tumefaciens. Nucleic Acids Res 18: 6909-6913. Quandt, J. & M. F. Hynes, (1993) Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteria. Gene 127: 15-21. Rogowsky, P. M., T. J. Close, J. A. Chimera, J. J. Shaw & C. I. Kado, (1987) Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58. J Bacteriol 169: 5101-5112. Sagulenko, V., E. Sagulenko, S. Jakubowski, E. Spudich & P. J. Christie, (2001) VirB7 lipoprotein is exocellular and associates with the Agrobacterium tumefaciens T pilus. J Bacteriol 183: 3642-3651 Sambrook, J. & D. W. Russell. (2001) Molecular cloning : a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. Schägger, H. & von Jagow, G. (1987) Tricine–sodium dodecyl sulfate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1– 100 kDalton. Ana Biochem 166:368–379. Scheiffele, P., W. Pansegrau & E. Lanka, (1995) Initiation of Agrobacterium tumefaciens T-DNA processing. Purified proteins VirD1 and VirD2 catalyze site- and strand-specific cleavage of superhelical T-border DNA in vitro. J Biol Chem 270: 1269-1276. Schmidt-Eisenlohr, H., N. Domke, C. Angerer, G. Wanner, P. C. Zambryski & C.Baron, (1999) Vir proteins stabilize VirB5 and mediate its association with the T pilus of Agrobacterium tumefaciens. J Bacteriol 181: 7485-7492. Schrammeijer, B., A. den Dulk-Ras, A. C. Vergunst, E. Jurado Jacome & P. J.Hooykaas, (2003) Analysis of Vir protein translocation from Agrobacterium tumefaciens using Saccharomyces cerevisiae as a model: evidence for transport of a novel effector protein VirE3. Nucleic Acids Res 31: 860-868. Shaw, C. H., (1991) Swimming against the tide: chemotaxis in Agrobacterium.Bioessays 13: 25-29. Shirasu, K. & C. I. Kado, (1993) Membrane location of the Ti plasmid VirB proteins involved in the biosynthesis of a pilin-like conjugative structure on Agrobacterium tumefaciens. FEMS Microbiol Lett 111: 287-294. Shirasu, K., Z. Koukolikova-Nicola, B. Hohn & C. I. Kado, (1994) An inner-membrane-associated virulence protein essential for T-DNA transfer from Agrobacterium tumefaciens to plants exhibits ATPase activity and similarities to conjugative transfer genes. Mol Microbiol 11: 581-588. Simon, R., U.Priefer & A. Puhler, (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Biotechnology 1: 784-791. Smith, E. F. & C. O. Townsend, (1907) A plant-tumor of bacterial origin. Science 24:671-673. Smith V.A.&J. Hindley (1978) Effect of agrocin 84 on attachment of Agrobacterium tumefaciens to cultured tobacco cells. Nature 276:498–500. Spudich, G. M., D. Fernandez, X. R. Zhou & P. J. Christie, (1996) Intermolecular disulfide bonds stabilize VirB7 homodimers and VirB7/VirB9 heterodimers during biogenesis of the Agrobacterium tumefaciens T-complex transport apparatus. Proc Natl Acad Sci U S A 93: 7512-7517. Stachel, S. E., E. Messens, M. Vanmontagu& P. Zambryski, (1985) Identification of signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens. Nature 318:624-629. Stachel, S. E. & E. W. Nester, (1986) The genetic and transcriptional organization of the vir region of the A6 Ti plasmid of Agrobacterium tumefaciens. EMBO J 5:1445-1454. Stachel, S. E., E. W. Nester & P. C. Zambryski, (1986) A plant cell factor induces Agrobacterium tumefaciens vir gene expression. Proc Natl Acad Sci U S A 83: 379-383. Stachel, S. E. & P. C. Zambryski, (1986) virA and virG control the plant-induced activation of the T-DNA transfer process of Agrobacterium tumefaciens. Cell 46: 325-333. Thomashow, M. F., J. E. Karlinsey, J. R. Marks & R. E. Hurlbert, (1987) Identification of a new virulence locus in Agrobacterium tumefaciens that affects polysaccharide composition and plant cell attachment. J Bacteriol 169:3209-3216. Thorstenson, Y. R., G. A. Kuldau & P. C. Zambryski, (1993) Subcellular localization of seven VirB proteins of Agrobacterium tumefaciens: implications for the formation of a T-DNA transport structure. J Bacteriol 175: 5233-5241. Thorstenson, Y. R. & P. C. Zambryski, (1994) The essential virulence protein VirB8 localizes to the inner membrane of Agrobacterium tumefaciens. J Bacteriol 176:1711-1717. Tombolini, R., A. Unge, M. E. Davey, F. J. deBruijn & J. K. Jansson, (1997) Flow cytometric and microscopic analysis of GFP-tagged Pseudomonas fluorescens bacteria. FEMS Microbiol Ecol 22:17-28. Turk, S. C. H. J., L. S. Melchers, H. den Dulk-Ras, A. J. A. Regensburg-Tuink,& P. J. J. Hooykass, (1991) Environmental conditions differentially affect vir gene induction in different Agrobacterium strains. Role of the VirA sensor protein. Plant Mol Biol 16: 1051–1059. Tzfira T., M. Vaidya & V.Citovsky, (2001) VIP1, an Arabidopsis protein that interacts with Agrobacterium VirE2, is involved in VirE2 nuclear import and Agrobacterium infectivity. EMBO J 20:3596-3607. Tzfira, T. & V. Citovsky, (2002) Partners-in-infection: host proteins involved in the transformation of plant cells by Agrobacterium. Trends Cell Biol 12: 121-129. Tzfira, T., M. Vaidya & V. Citovsky, (2004) Involvement of targeted proteolysis in plant genetic transformation by Agrobacterium. Nature 431: 87-92. Tzfira, T., B. Lacroix & V. Citovsky, (2005). Nuclear import of Agrobacterium T-DNA. in: Nuclear import and export./T. Tzfira and V. Citovsky (eds.), Landes Bioscience and Kluwer Academic. 83-99. Tzfira, T. & V. Citovsky, (2006) Agrobacterium-mediated genetic transformation of plants: biology and biotechnology. Curr Opin Biotechnol 17: 147-154. Vergunst, A. C., B. Schrammeijer, A. den Dulk-Ras, C. M. de Vlaam, T. J.Regensburg-Tuink & P. J. Hooykaas, (2000) VirB/D4-dependent protein translocation from Agrobacterium into plant cells. Science 290: 979-982. Vergunst, A. C., M. C. van Lier, A. den Dulk-Ras & P. J. Hooykaas, (2003)Recognition of the Agrobacterium tumefaciens VirE2 translocation signal by the VirB/D4 transport system does not require VirE1. Plant Physiol 133: 978-988. Wang K., S.E.Stachel, B.Timmerman, M.Van Montagu & P.C. Zambryski (1987) Site-specific nick in the T-DNA border sequence as a result of Agrobacterium vir gene expression. Science 235: 587–591. Ward E.R. & Barnes W.M.(1988) VirD2 protein of Agrobacterium tumefaciens very tightly linked to the 5' end of T-strand DNA. Science 242:927–930. Ward, J. E., Jr., E. M. Dale, E. W. Nester & A. N. Binns, (1990) Identification of a VirB10 protein aggregate in the inner membrane of Agrobacterium tumefaciens. J Bacteriol 172: 5200-5210. Ward, D. V., J. R. Zupan & P. C. Zambryski, (2002) Agrobacterium VirE2 gets the VIP1 treatment in plant nuclear import. Trends Plant Sci 7: 1-3.White, C. E. & S. C. Winans, (2007) Cell-cell communication in the plant pathogen Agrobacterium tumefaciens. Philos Trans R Soc Lond B Biol Sci 362: 1135-1148. Winans, S. C., (1990) Transcriptional induction of an Agrobacterium regulatory gene at tandem promoters by plant-released phenolic compounds, phosphate starvation,and acidic growth media. J Bacteriol 172: 2433-2438. Winans, S. C., (1992) Two-way chemical signaling in Agrobacterium-plant interactions.Microbiol Rev 56: 12- 31. Wood, D. W., J. C. Setubal, R. Kaul, D. E. Monks, J. P. Kitajima, V. K. Okura,Y. Zhou, L. Chen, G. E. Wood, N. F. Almeida, Jr., L. Woo, Y. Chen, I. T. Paulsen, J. A. Eisen, P. D. Karp, D. Bovee, Sr., P. Chapman, J. Clendenning, G.Deatherage, W. Gillet, C. Grant, T. Kutyavin, R. Levy, M. J. Li, E. McClelland, A. Palmieri, C. Raymond, G. Rouse, C. Saenphimmachak, Z. Wu, P. Romero,D. Gordon, S. Zhang, H. Yoo, Y. Tao, P. Biddle, M. Jung, W. Krespan, M.Perry, B. Gordon- Kamm, L. Liao, S. Kim, C. Hendrick, Z. Y. Zhao, M. Dolan,F. Chumley, S. V. Tingey, J. F. Tomb, M. P. Gordon, M. V. Olson & E. W.Nester, (2001) The genome of the natural genetic engineer Agrobacterium tumefaciens C58. Science 294: 2317-2323. Wu, H. Y., P. C. Chung, H. W. Shih, S. R. Wen & E. M. Lai, (2008) Secretome analysis uncovers an Hcp-family protein secreted via a type VI secretion system in Agrobacterium tumefaciens. J Bacteriol 190: 2841-2850. Yadav, N. S., J. Vanderleyden, D. R. Bennett, W. M. Barnes & M. D. Chilton, (1982) Short direct repeats flank the T-DNA on a nopaline Ti plasmid. Proc Natl Acad Sci U S A 79: 6322-6326. Yuan, Q., A. Carle, C. Gao, D. Sivanesan, K. A. Aly, C. Hoppner, L. Krall, N. Domke & C. Baron, (2005) Identification of the VirB4-VirB8-VirB5-VirB2 pilus assembly sequence of type IV secretion systems. J Biol Chem 280: 26349-26359. Yuan, Z. C., P. Liu, P. Saenkham, K. Kerr & E. W. Nester, (2008) Transcriptome profiling and functional analysis of Agrobacterium tumefaciens reveals a general conserved response to acidic conditions (pH 5.5) and a complex acid-mediated signaling involved in Agrobacterium-plant interactions. J Bacteriol 190: 494-507. Zaenen, I., N. Van Larebeke, M. Van Montagu & J. Schell, (1974) Supercoiled circular DNA in crown-gall inducing Agrobacterium strains. J Mol Biol 86: 109- 127. Zambryski, P., H. Joos, C. Genetello, J. Leemans, M. Van Montagu & J. Schell (1983) Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity. EMBO J 2:2143–2150. Zhu, J., P. M. Oger, B. Schrammeijer, P. J. Hooykaas, S. K. Farrand & S. C. Winans,(2000) The bases of crown gall tumorigenesis. J Bacteriol 182: 3885-3895. Zhu, Y., J. Nam, J. M. Humara, K. S. Mysore, L. Y. Lee, H. Cao, L. Valentine, J. Li, A. D. Kaiser, A. L. Kopecky, H. H. Hwang, S. Bhattacharjee, P. K. Rao, T. Tzfira, J.Rajagopal, H. Yi, Veena, B. S. Yadav, Y. M. Crane, K. Lin, Y. Larcher, M. J. Gelvin, M. Knue, C. Ramos, X. Zhao, S. J. Davis, S. I. Kim, C. T. Ranjith-Kumar, Y. J. Choi, V. K. Hallan, S. Chattopadhyay, X. Sui, A.Ziemienowicz, A. G. Matthysse, V. Citovsky, B. Hohn & S. B. Gelvin, (2003)Identification of Arabidopsis rat mutants. Plant Physiol 132: 494-505. Zupan, J., C. A. Hackworth, J. Aguilar, D. Ward & P. Zambryski, (2007) VirB1* promotes T-pilus formation in the vir-Type IV secretion system of Agrobacterium tumefaciens. J Bacteriol 189: 6551-6563. Zupan, J., T. R. Muth, O. Draper & P. Zambryski, (2000) The transfer of DNA from Agrobacterium tumefaciens into plants: a feast of fundamental insights. Plant J 23: 11-28. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/40257 | - |
dc.description.abstract | 農桿菌是一植物病原細菌,其可感染大多數的雙子葉植物,產生腫瘤的病徵;腫瘤的產生係由於農桿菌會將其T-DNA (Transferred DNA) 切下並送至植物基因組中,而將植物細胞轉形為腫瘤。當植物細胞受傷時會釋出酚類化合物(例如acetosyringone,AS),進而誘導農桿菌ㄧ巨大質體Ti (tumor-inducing) 質體之毒性基因 (vir genes) 的表現,其表現係由VirA/VirG two-component 系統所調控,故稱為vir regulon。 vir regulon中之virB操縱子包含 (virB1~virB11)會轉譯出11個VirB蛋白質,與VirD4組成第四型分泌系統,負責T-DNA及其他基質的轉移。農桿菌培養於含AS之酸性AB-MES minimal培養基中可有效地誘導毒性基因在轉錄層級之表現,但在酸性rich培養基中是否可有效地誘導其研究結果卻不一致,而對於毒性基因在後轉錄層級的調控也未有系統性的分析。所以,本論文利用已知適合毒性基因誘導的酸性AB-MES minimal培養基,以及選用酸性的523 rich培養基當作控制組,針對virB毒性基因受AS誘導表現之轉錄、轉譯及蛋白質穩定狀態進行探討,期了解virB基因是否在不同培養基中受到不同層級的調控。首先,利用轉錄融合分析毒性基因啟動子的活性,發現virB、virD及virE啟動子在AB-MES及523培養基皆可有效地進行轉錄,但在AB-MES中較523培養基在AS誘導前期有較高的表現;進一步針對virB毒性基因之表現再使用西方雜合反應分析VirB蛋白質穩定狀態,發現不同的VirB蛋白質在不同培養基中累積的狀況不同,可將其概分為三群。第一群包含VirB2、VirB7及VirB9,此群蛋白質在AS誘導前期(16小時前)在AB-MES中之累積量較高於其在523中之累積量;第二群包含VirB1、VirB4、VirB5、VirB8及VirB11,此群蛋白質在AS誘導後期 (24小時後) 在AB-MES中之累積量較高於523中之累積量;第三群包含VirB3及VirB10,此群蛋白質在AB-MES中之累積量遠高於其在523中之累積量。再透過轉譯融合分析VirB蛋白質轉譯效率,與virB啟動子活性比較,發現轉譯融合菌株除了VirB1-GFP無法偵測到GFP螢光值,其餘VirB2-GFP、VirB3-GFP及VirB10-GFP皆能有效地被AS誘導表現,推測當農桿菌523培養基受AS誘導時,可有效率地轉錄及轉譯出VirB蛋白質,但不同之VirB蛋白質則有不同的穩定度。利用IncQ質體RSF1010經由VirB/D4在農桿菌間之轉移試驗,發現RSF1010在523培養基的轉移效率極低,為在AB-MES培養基之0.41%,推測在523培養基RSF1010之低轉移效率可能係由於部分VirB蛋白質之低累積量,進而無法組合成有功能之T4SS來進行DNA之轉移。 | zh_TW |
dc.description.abstract | Agrobacterium tumefaciens is a plant pathogenic bacterium, the causal agent of crown gall diseases on wide range of dicotyledons. The tumors are caused by transferring T-DNA (Transferred DNA) from bacterium into the host plant genome. A. tumefaciens is capable of sensing the plant-released signal molecules such as sugars and phenolic compounds (e.g. acetosyringone,AS) to activate the expression of virulence (vir) genes encoded by the tumor-inducing (Ti) plasmid. Among these, the virB operon encoding 11 VirB proteins and VirD4 comprises a type IV secretion system (T4SS) to transfer T-DNA and effectors from bacteria into the host plant cells. AS-induced vir gene expression is regulated at transcriptional level via VirA/VirG with maximal activity in acidic AB-MES minimal medium. However, it is not clear whether vir genes can be efficiently induced by AS when grown in rich medium or are regulated at posttranscriptional levels. In this study, we aim to understand whether virB genes are expressed and regulated differently when A. tumefaciens is grown in different culture media for AS induction. The virB gene expression was analyzed at transcriptional/translational levels and protein steady state when induced by AS in both acidic AB-MES minimal medium and acidic 523 rich medium. By transcriptional fusion to gfp (green fluorescent protein), the promoter activities of virB, virD, and virE are efficiently induced when grown in either AB-MES or 523 media although the promoter activities are higher in AB-MES medium than in 523 medium at early time points. To further investigate virB gene expression at protein levels, Western blotting and translational fusions were carried out. By Western blot analysis, three groups of VirB proteins are classified based on their protein accumulation patterns in both AB-MES and 523 cultures. VirB2, VirB7, and VirB9 belong to group I as they accumulate at higher levels at early time points (up to 16 hr) in AB-MES in comparison to those in 523. The second group of proteins include VirB1, VirB4, VirB5, VirB8, and VirB11 as they accumulate at higher levels at late time points (after 24 hr) in AB-MES in comparison to those in 523. The third group includes VirB3 and VirB10 as they accumulate at very low levels in 523, in contrast to those at much higher levels in AB-MES. Interestingly, several VirB-GFP translational fusions result in efficient AS-induced activity in both AB-MES and 523 media. By VirB/D4-mediated IncQ plasmid RSF1010 transfer between A. tumefaciens strains, only very little transfer events are detected when conjugation was performed on 523. In conclusions, our data suggest that virB is transcribed and translated efficiently when A. tumefaciens was induced by AS in both AB-MES and 523 media. However, while all tested VirB proteins accumulated stably when grown in AB-MES medium, certain VirB proteins are less stable when grown in 523 medium, which may lead to the failure in assembly of the functional T4SS for DNA transfer. | en |
dc.description.provenance | Made available in DSpace on 2021-06-14T16:43:31Z (GMT). No. of bitstreams: 1 ntu-97-R95633007-1.pdf: 709459 bytes, checksum: b352af3eb4904011316d42a218bc03c8 (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 口試委員會審定書....................................I
誌謝...............................................II 中文摘要..........................................III 英文摘要............................................V 表目錄...........................................VIII 圖目錄.............................................IX 壹、前言............................................1 一、農桿菌簡介...................................1 二、農桿菌研究的重要里程碑.......................1 三、農桿菌感染植物的分子機制.....................3 四、農桿菌毒性基因的表現及調控之研究.............8 貳、研究目的.......................................11 参、研究材料與方法.................................12 肆、研究結果.......................................24 一、農桿菌在不同培養基其virB基因表現的分析......24 二、 IncQ質體 RSF1010的轉移效率試驗.............30 伍、討論...........................................31 參考文獻...........................................36 附錄...............................................70 附錄圖.............................................75 | |
dc.language.iso | zh-TW | |
dc.title | 農桿菌virB基因表現及調控之研究 | zh_TW |
dc.title | Expression and regulation analyses of virB genes in
Agrobacterium tumefaciens | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 陳昭瑩(Chao-Ying Chen) | |
dc.contributor.oralexamcommittee | 黃皓瑄(Hau-Hsuan Hwang) | |
dc.subject.keyword | 農桿菌,酚類化合物,virB毒性基因,基因表現,基因調控,第四型分泌系統, | zh_TW |
dc.subject.keyword | Agrobacterium tumefaciens,acetosyringone,vir regulon,gene expression,gene regulation,type IV secretion system, | en |
dc.relation.page | 84 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2008-08-01 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
顯示於系所單位: | 植物病理與微生物學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-97-1.pdf 目前未授權公開取用 | 692.83 kB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。