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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃慶璨(Ching-Tsan Huang) | |
| dc.contributor.author | Chian-Wan Lin | en |
| dc.contributor.author | 林千椀 | zh_TW |
| dc.date.accessioned | 2021-06-14T16:41:41Z | - |
| dc.date.available | 2013-08-04 | |
| dc.date.copyright | 2008-08-04 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-08-01 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/40138 | - |
| dc.description.abstract | 靈芝具有抗腫瘤、抗發炎、增強免疫力及許多生理上的療效主要是因多醣體、三萜類及小分子蛋白質等活性物質。小分子蛋白質最早是由靈芝 (Ganoderma lucidum) 的菌絲體中分離，為一個分子量 12.4 kDa 且具有免疫調節活性的物質，命名為 LZ-8，原態是以同源雙體的形式存在。後來由不同真菌菌絲體分離出與 LZ-8 序列相近的免疫調節蛋白質，通稱為 FIPs (fungal immunomodulatory proteins)，GMI為本實驗室由小孢子靈芝 (Ganoderma microsporum) 菌絲體選殖之免疫調節蛋白質。LZ-8 與 GMI 目前已被證實具有抗自體免疫疾病及糖尿病等療效，經過 LZ-8 處理的人類周邊淋巴細胞會被誘導而分泌 IL-2、IL-4 及 IFN- | zh_TW |
| dc.description.abstract | The activity of anti-tumor, anti-inflammentary and increasing immunity of Ganoderme lucidum were resulted from its major components, polysaccharide, triterperoid and immunomodulatory protein. The first immunomodulatory protein, know as LZ-8, was isolated from the mycelium of Ganoderma lucidum. The formation of homodimer is necessary for LZ-8 to exhibit immunomodulatory activity. GMI is another immunomodulatory protein gene cloned from Ganoderma microsporum.
The immune activity of LZ-8 was closely related to the 5th, 7th and 9th amino acids, leucine, phenylalanine and leucine, respectively, in | en |
| dc.description.provenance | Made available in DSpace on 2021-06-14T16:41:41Z (GMT). No. of bitstreams: 1 ntu-97-R95b47401-1.pdf: 1518419 bytes, checksum: 90c52339533de0a37d8de1dd87f545c2 (MD5) Previous issue date: 2008 | en |
| dc.description.tableofcontents | 目錄 I
表目錄 IV 圖目錄 V 摘要 VII Abstract VIII 第一章 前言 1 一、靈芝 1 二、免疫調節蛋白質 4 1. 靈芝免疫調節蛋白質 4 1.1 LZ-8 4 1.2 FIP-gts 5 1.3 GMI 6 1.4 生理活性 6 2. 其他真菌免疫調節蛋白質 9 2.1 FIP-fve 與 FIP-vvo 9 2.2 蛋白質結構 9 三、分子演化技術 12 四、異源表達系統 13 五、Pichia pastoris 表達系統 16 1. 篩選標誌 16 1.1 營養需求篩選標誌 17 1.2 抗藥性篩選標誌 17 2. 增加異源蛋白質產量 18 2.1 啟動子 19 2.2 蛋白質酶缺陷株 19 2.3 拷貝數 20 六、研究動機與目的 21 第二章 材料與方法 24 一、 實驗材料 24 1. 菌株與培養條件 24 1.1 細菌 24 1.2 真菌 24 2. 細胞株與培養條件 24 3. 質體與引子 25 3.1 pPICZaA 25 3.2 pGW 25 3.3 pPGMI 25 3.4 pPGMI-L6C 25 3.5 pPGMI-L10C 25 3.6 pPGMI-L6C&L10C 26 3.7 引子 26 二、 實驗方法 27 1. 靈芝免疫調節蛋白質胺基酸序列突變 27 1.1 突變位置預測 27 1.2 質體建構 27 1.3 gmi-L6C、gmi-L10C 及 gmi-L6C&L10C 27 2. 靈芝免疫蛋白質於 P. pastoris 之異源表現 28 2.1 pGMI-L6C、pGMI-L10C、pGMI-L6C&L10C 表現載體之建構 28 2.2 P. pastoris 電穿孔轉形法 28 2.3 P. pastoris 轉形株之篩選 29 2.4 P. pastoris 異源蛋白質之表現 30 2.5 P. pastoris 異源蛋白質之濃縮與純化 30 3. P. pastoris 重組蛋白質之分析 31 3.1 蛋白質定量 31 3.2 ELISA 分析 31 3.3 CBR 染色及西方轉印 32 3.4 免疫調節活性測試 33 3.5 蛋白質單體/雙體 (monomer/dimer) 之測定 34 4. Trypan blue 染劑排除法 34 第三章 結果 35 一、 嗜甲醇酵母菌轉形系統的建構 35 1. 突變型 GMI 之質體建構 35 1.1 突變引子設計 35 1.2 GMI-L6C、GMI-L10C及GMI-L6C& pGMI-L10C基因釣取 35 1.3 pGMI-L6C、pGMI-L10C及pGMI-L6C& pGMI-L10C構築 36 2. 突變型 GMI 轉形株篩選 37 3. 野生型及突變型 GMI 蛋白質純化 37 二、 蛋白質分析 39 1. 西方雜合 39 2. 野生型及突變型 GMI 蛋白質的免疫活性 40 三、 條件測試 41 1. 溫度與時間 41 2. 試劑 41 第四章 討論 43 一、蛋白質分析 43 二、條件測試 45 1. 溫度與時間 45 2. 試劑 45 第五章 總結 46 第六章 未來展望 47 第七章 表與圖 48 參考文獻 79 | |
| dc.language.iso | zh-TW | |
| dc.subject | Cys | zh_TW |
| dc.subject | LZ-8 | zh_TW |
| dc.subject | 同源雙體 | zh_TW |
| dc.subject | fungal immunomodulatory protein | zh_TW |
| dc.subject | GMI | zh_TW |
| dc.subject | IL-2 | zh_TW |
| dc.subject | L5/F7/L9 | zh_TW |
| dc.subject | L6/L10 | zh_TW |
| dc.subject | homodimer | en |
| dc.subject | GMI | en |
| dc.subject | fungal immunomodulatory protein | en |
| dc.subject | LZ-8 | en |
| dc.subject | Cys | en |
| dc.subject | L6/L10 | en |
| dc.subject | L5/F7/L9 | en |
| dc.subject | IL-2 | en |
| dc.title | 利用分子演化增加小孢子靈芝選殖之免疫調節蛋白質 GMI 的免疫活性 | zh_TW |
| dc.title | Enhancing of the immune activity of an immunomodulatory protein, GMI, from Ganoderma lucidum, by direct evolution | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 許瑞祥(Ruey-Shyang Hseu),楊啟伸(Chii-Shen Yang),張麗冠(Li-Kwan Chang),陳浩仁(Hau - Ren Chen) | |
| dc.subject.keyword | LZ-8,同源雙體,fungal immunomodulatory protein,GMI,IL-2,L5/F7/L9,L6/L10,Cys, | zh_TW |
| dc.subject.keyword | LZ-8,homodimer,fungal immunomodulatory protein,GMI,IL-2,L5/F7/L9,L6/L10,Cys, | en |
| dc.relation.page | 84 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2008-08-01 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
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