請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/39187完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 章良渭 | |
| dc.contributor.author | Ching-Yuan Wu | en |
| dc.contributor.author | 吳青原 | zh_TW |
| dc.date.accessioned | 2021-06-13T17:24:08Z | - |
| dc.date.available | 2005-02-16 | |
| dc.date.copyright | 2005-02-16 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-01-27 | |
| dc.identifier.citation | Bombaro, K. M., L. H. Engrav, et al. (2003). 'What is the prevalence of hypertrophic scarring following burns?' Burns 29(4): 299-302.
Cheng, J. C., J. H. Evans, et al. (1984). 'Pressure therapy in the treatment of post-burn hypertrophic scar--a critical look into its usefulness and fallacies by pressure monitoring.' Burns Incl Therm Inj 10(3): 154-63. Chipev, C. C. and M. Simon (2002). 'Phenotypic differences between dermal fibroblasts from different body sites determine their responses to tension and TGFbeta1.' BMC Dermatol 2(1): 13. Clark, J. A., J. C. Cheng, et al. (1996). 'Mechanical properties of normal skin and hypertrophic scars.' Burns 22(6): 443-6. Clark, J. A., J. C. Cheng, et al. (1987). 'Mechanical characterisation of human postburn hypertrophic skin during pressure therapy.' J Biomech 20(4): 397-406. Clark, J. A., K. S. Leung, et al. (1996). 'The hypertrophic scar and microcirculation properties.' Burns 22(6): 447-50. Daniels, J. T., G. S. Schultz, et al. (2003). 'Mediation of transforming growth factor-beta(1)-stimulated matrix contraction by fibroblasts: a role for connective tissue growth factor in contractile scarring.' Am J Pathol 163(5): 2043-52. Demling, R. H. and L. DeSanti (2001). 'Scar management strategies in wound care.' Rehab Manag 14(6): 26-30. Dimitrijevich, S. D., S. Paranjape, et al. (1999). 'Effect of hyperbaric oxygen on human skin cells in culture and in human dermal and skin equivalents.' Wound Repair Regen 7(1): 53-64. Eastwood, M., D. A. McGrouther, et al. (1998). 'Fibroblast responses to mechanical forces.' Proc Inst Mech Eng [H] 212(2): 85-92. Eastwood, M., V. C. Mudera, et al. (1998). 'Effect of precise mechanical loading on fibroblast populated collagen lattices: morphological changes.' Cell Motil Cytoskeleton 40(1): 13-21. Hirano, S., D. M. Bless, et al. (2003). 'Morphological and functional changes of human vocal fold fibroblasts with hepatocyte growth factor.' Ann Otol Rhinol Laryngol 112(12): 1026-33. Kossi, J., M. Vaha-Kreula, et al. (2004). 'Hexose sugars differentially alter collagen gene expression and synthesis in fibroblasts derived from granulation tissue, hypertrophic scar and keloid.' Arch Dermatol Res. Krotzsch-Gomez, F. E., J. Furuzawa-Carballeda, et al. (1998). 'Cytokine expression is downregulated by collagen-polyvinylpyrrolidone in hypertrophic scars.' J Invest Dermatol 111(5): 828-34. Kuroyanagi, Y., K. Kubo, et al. (2004). 'Establishment of banking system for allogeneic cultured dermal substitute.' Artif Organs 28(1): 13-21. Leung, K. S., J. C. Cheng, et al. (1984). 'Complications of pressure therapy for post-burn hypertrophic scars. Biomechanical analysis based on 5 patients.' Burns Incl Therm Inj 10(6): 434-8. Leung, K. S., A. Sher, et al. (1989). 'Microcirculation in hypertrophic scars after burn injury.' J Burn Care Rehabil 10(5): 436-44. Martin, P. (1997). 'Wound healing--aiming for perfect skin regeneration.' Science 276(5309): 75-81. Moulin, V., S. Larochelle, et al. (2004). 'Normal skin wound and hypertrophic scar myofibroblasts have differential responses to apoptotic inductors.' J Cell Physiol 198(3): 350-8. Nedelec, B., A. Ghahary, et al. (2000). 'Control of wound contraction. Basic and clinical features.' Hand Clin 16(2): 289-302. Nikkonen, M. M., J. M. Pitkanen, et al. (2001). 'Problems associated with the use of silicone gel sheeting for hypertrophic scars in the hot climate of Saudi Arabia.' Burns 27(5): 498-501. Nissen, N. N., R. L. Gamelli, et al. (2003). 'Differential angiogenic and proliferative activity of surgical and burn wound fluids.' J Trauma 54(6): 1205-10; discussion 1211. Pohlman, T. H. and J. M. Harlan (2000). 'Adaptive responses of the endothelium to stress.' J Surg Res 89(1): 85-119. Reno, F., M. Sabbatini, et al. (2003). 'In vitro mechanical compression induces apoptosis and regulates cytokines release in hypertrophic scars.' Wound Repair Regen 11(5): 331-6. Rivers, E. A., R. G. Strate, et al. (1979). 'The transparent face mask.' Am J Occup Ther 33(2): 108-13. Roques, C. (2002). 'Massage applied to scars.' Wound Repair Regen 10(2): 126-8. Roques, C. (2002). 'Pressure therapy to treat burn scars.' Wound Repair Regen 10(2): 122-5. Shin, D. and K. W. Minn (2004). 'The effect of myofibroblast on contracture of hypertrophic scar.' Plast Reconstr Surg 113(2): 633-40. Tredget, E. E., B. Nedelec, et al. (1997). 'Hypertrophic scars, keloids, and contractures. The cellular and molecular basis for therapy.' Surg Clin North Am 77(3): 701-30. Zhu, K. Q., L. H. Engrav, et al. (2004). 'Further similarities between cutaneous scarring in the female, red Duroc pig and human hypertrophic scarring.' Burns 30(6): 518-30. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/39187 | - |
| dc.description.abstract | 前言：壓力療法，是臨床用以治療疤痕中，最常見的非侵入式療法，如何去改善其療效，並增進病人的舒適度，是本研究的動機。透過對壓力療法以及肥厚性疤痕的瞭解，瞭解各種療效的不同假設，並選擇「抑制膠原纖維的堆積」作為本實驗的基礎假設，並以「疤痕纖維母細胞」作為主要的研究對象，討論其生長與活性，其中活性針對生長因子TGF-beta1為主。期望可以由此方面證明壓力的確有助於抑制纖維母細胞的生長與活性，而達到改善疤痕的效果。
材料與方法：在壓力的施加上，選擇以氣壓的方式來進行，並自行設定了一個可以自主控制施加臨床治療壓力20至40毫米汞柱的加壓儀器。實驗中所選擇的參數是：「壓力」、「加壓週期」。而進行評估的方式是：生長曲線以及酵素連結免疫吸附分析，生長曲線反應該細胞的生長情況，而酵素連結免疫吸附分析則是為了瞭解生長因子TGF-beta1的分泌情況。 結果與討論：從實驗後結果顯示，壓力的確造成細胞數量上的明顯下降，不過不同的氣壓之下，彼此間的不同，存在於影響細胞所需要的加壓週期，20毫米汞柱的壓力需要全天的時間，而40毫米汞柱的壓力只需要每天18小時，就可以達到同樣的效果，而且在延長加壓40毫米拱柱的時間，卻沒有進一步的影響。在生長因子TGF-beta1的結果上，也呈現相同的效果。 結論：壓力如預期般可以抑制疤痕纖維母細胞的生長與活性，並且壓力與加壓週期中存在一定的關係，壓力越大，所需的週期愈小。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-13T17:24:08Z (GMT). No. of bitstreams: 1 ntu-94-R91548033-1.pdf: 1919440 bytes, checksum: 09aa732cf2c955c7bc701bba275bbd22 (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 總目錄
致謝 II 摘要 III Abstract IV 總目錄 V 圖目錄 VII 表目錄 IX 第一章 緒論 1 1.1研究動機 1 1.2文獻回顧 2 1.2.1壓力治療 3 1.2.2傷口癒合 4 1.2.3肥厚性疤痕 8 1.3研究目的 11 第二章 材料與方法 13 2.1實驗設備 13 2.2實驗方法 15 2.2.1實驗細胞 15 2.2.2生長曲線 17 2.2.3生長激素檢定 18 2.3 藥品配置 20 2.3.1 DMEM培養基配置方法 20 2.3.2 PBS配置方法 20 第三章 實驗流程 22 3.1參數設定 24 3.2實驗流程 25 第四章 結果與討論 28 4.1生長曲線 28 4.1.2壓力控制 30 4.1.2週期控制 32 4.2 生長激素檢定 37 4.2.1 壓力控制 39 4.2.2週期控制 40 第五章 結論與未來工作 44 5.1結論 44 5.2未來工作 44 參考文獻 46 | |
| dc.language.iso | zh-TW | |
| dc.subject | 生長 | zh_TW |
| dc.subject | 疤痕 | zh_TW |
| dc.subject | 壓力 | zh_TW |
| dc.subject | 纖維母細胞 | zh_TW |
| dc.subject | TGF-beta | en |
| dc.subject | scar | en |
| dc.subject | fibroblast | en |
| dc.subject | proliferation | en |
| dc.subject | pressure | en |
| dc.title | 壓力環境對於疤痕組織纖維母細胞之影響 | zh_TW |
| dc.title | Pressure Effects on the Growth of the Human Skin Fibroblast | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 楊永健,鄧文炳 | |
| dc.contributor.oralexamcommittee | 戴浩志 | |
| dc.subject.keyword | 疤痕,壓力,纖維母細胞,生長, | zh_TW |
| dc.subject.keyword | scar,fibroblast,pressure,TGF-beta,proliferation, | en |
| dc.relation.page | 49 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-01-27 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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