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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 朱元南(Yuan-Nan Chu) | |
| dc.contributor.author | Guo – Cheng He | en |
| dc.contributor.author | 何國誠 | zh_TW |
| dc.date.accessioned | 2021-06-13T17:06:11Z | - |
| dc.date.available | 2010-02-02 | |
| dc.date.copyright | 2005-02-02 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-01-27 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/39174 | - |
| dc.description.abstract | 本論文研究薄層聚合酵素連鎖反應(PCR)系統及螢光檢測系統，薄層聚合酵素連鎖反應系統之反應槽以PDMS做為材質，並且以單一鑄模方式製作，製程簡單且具有高製造良率。昇降溫部份以熱電致冷晶片做為加熱元件，搭配離心風扇做強制對流泠却。溫控部份以LabVIEW軟體擷取Thermocouple量測的溫度資料，再進行PID控制以改變TEC的加熱功率輸出。經實驗證明此薄層聚合酵素連鎖系統可成功的複製DNA序列。螢光檢測系統分為二部份裝置，雷射誘導螢光檢測部份以532nm 波長的綠光半導體雷射做為激發光源，搭配適當光學元件，最後以光電倍增管偵測樣本螢光訊號，並以 LabVIEW 軟體程式做為資料擷取系統。螢光影像系統則以UV光做為DNA染料激發光源，再以CCD擷取螢光影像。本螢光檢測系統利用樣本步進平台可作定點或掃描之螢光偵測，達成多用型螢光檢測系統之目的，實驗證實可成功的偵測DNA螢光訊號。 | zh_TW |
| dc.description.abstract | A thin-film Polymerase Chain Reaction (TF-PCR) system and a fluorescence detection system have been developed. Made of PDMS, the TF-PCR is fabricated by casting with easy of manufacturing and very high quality. The TF-PCR contains thermoelectric cooling components (TEC) as heaters, and uses a centrifuge system fan for forced convention cooling. The temperature controlling system is composed of a thermocouple as a temperature sensor, a DAQ card gather to voltage signals and a PC with the LabVIEW program. The monitoring and controlling of the TEC heater is through a PID program. Successful amplification of DNA strains of a shrimp virus with 120 and 226 base pair has been demonstrated by this TF-PCR. The fluorescence detection system can be divided into two subsystems. The LASER-induced fluorescence detecting subsystem includes a Green color semiconductor LASER (532nm in wavelength) as an exciting light source, followed by proper optical components and a photo multiplying tube (PMT), then fluorescence signals from DNA samples can be caught. The fluorescence image subsystem has a UV light source and can extract images from a CCD camera. This fluorescence detection system also uses step motors to control the sample stand, so that fixed scanning or moved scanning is possible. This fluorescence detection system is a multiple functional detecting system. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T17:06:11Z (GMT). No. of bitstreams: 1 ntu-94-R91631006-1.pdf: 1667023 bytes, checksum: fd17d3198566d77d9817cac44174131b (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 目 錄
誌 謝 I 中文摘要 II 英文摘要 III 目 錄 IV 圖目錄 VI 表目錄 VII 第一章 前言與研究目的 1 第二章 文獻探討 2 2-1 電泳簡介 2 2-2 電泳分離模式 3 2-2-1 平板凝膠電泳(Slab Gel Electrophoresis) 3 2-2-2 毛細管電泳(Capillary Electrophoresis， CE) 3 2-2-3 毛細管凝膠電泳(Capillary Gel Electrophoresis，CGE) 4 2-2-4 毛細管區帶電泳(Capillary Zone Electrophoresis，CZE) 4 2-3 毛細管電泳原理 5 2-3-1 電泳淌度 5 2-3-2 電滲透流(Electroosmotic flow ，EOF)的產生 7 2-4 分離效能評估 9 2-5 電泳檢測 10 2-5-1 吸收光譜偵測法 11 2-5-2 電化學偵測法 11 2-5-3 螢光偵測法 12 2-5-4 螢光染料分子 13 2-6 PCR簡介 14 2-6-1 PCR原理 14 2-7 相關應用 18 2-7-1 PMMA相關應用 19 2-7-2 PDMS相關應用 19 2-7-3 PCR-CE相關應用 22 第三章 研究設備與材料方法 25 3-1 電泳晶片 25 3-1-1 光罩設計與製作 25 3-1-2 晶片電極製程 26 3-1-3 清潔(Clean) 27 3-1-4 金屬薄膜沉積 (Deposit ) 27 3-1-5 Lift-Off 製程 28 3-2 高分子晶片製程 29 3-2-1 玻璃母模之製程 30 3-2-2 PDMS晶片製作 32 3-2-3 PMMA晶片製作 33 3-3 聚合酵素連鎖反應實驗操作方法 34 3-4 洋菜膠電泳實驗操作方法 36 3-4-1 儀器用具 36 3-4-2 藥品及試劑 36 3-4-3 實驗步驗 37 3-5 薄層PCR系統 38 3-5-1 PCR反應晶片 39 3-5-2 薄層PCR系統操作步驟 40 3-5-3 昇降溫裝置 41 3-5-4 溫度控制系統 41 3-5-5 溫度量測裝置 43 3-6 螢光檢測系統 43 3-6-1 螢光檢測影像系統 44 3-6-2 雷射誘導螢光檢測系統裝置 44 3-6-3 光電子倍增管原理 46 3-6-4 訊號擷取與分析系統 46 3-7 步進平台系統 47 第四章 結果與討論 49 4-1 薄層PCR系統 49 4-2 螢光偵測系統之背景雜訊對樣本檢測之影響 51 4-3 螢光樣本濃度梯度訊號 52 4-4 電泳分離螢光檢測訊號 53 第五章 結論 56 參考文獻 57 | |
| dc.language.iso | zh-TW | |
| dc.subject | 聚合酵素連鎖反應:螢光檢測:凝膠電泳 | zh_TW |
| dc.subject | Polymerase chain reaction (PCR):fluorescence detection: gel electrophoresis | en |
| dc.title | 薄層聚合酵素連鎖反應系統與螢光檢測系統之開發 | zh_TW |
| dc.title | Development of a Thin Film Polymerase Chain Reaction and Fluorescence Detection System | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳力騏(Richie Chen),艾群(Chyung Ay) | |
| dc.subject.keyword | 聚合酵素連鎖反應:螢光檢測:凝膠電泳, | zh_TW |
| dc.subject.keyword | Polymerase chain reaction (PCR):fluorescence detection: gel electrophoresis, | en |
| dc.relation.page | 59 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-01-28 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 生物產業機電工程學研究所 | zh_TW |
| 顯示於系所單位： | 生物機電工程學系 | |
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