請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/38525
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林萬寅 | |
dc.contributor.author | Yu-Ting Tai | en |
dc.contributor.author | 戴郁庭 | zh_TW |
dc.date.accessioned | 2021-06-13T16:36:15Z | - |
dc.date.available | 2005-07-11 | |
dc.date.copyright | 2005-07-11 | |
dc.date.issued | 2005 | |
dc.date.submitted | 2005-07-07 | |
dc.identifier.citation | 1. Campbell, A. K., Chemiluminescence: Principles and Applications in Biology and Medicine. New York, 1988; p 19-67.
2. Roda, A.; Guardigli, M.; Michelini, E.; Mirasoli, M.; Pasini, P., Anal. Chem. 2003, 75(21), 462A-470A. 3. Seitz, W. R.; Neary, M. P., Anal. Chem. 1974, 46(2), A188-A200. 4. Wilson, E., Chem. Eng. News 1999, 77(3), 65-65. 5. Albrecht, H. O., Physical chemistry & chemical physics 1928, 136, 321-321. 6. Gundermann, K.-D.; McCapra, F., Chemiluminescence in organic chemistry. 1986; p 89-92. 7. White, E. H.; Bursey, M. M., J. Am. Chem. Soc. 1964, 86, 941-942. 8. White, E. H.; Zafiriou, O.; Kagi, H. H.; Hill, J. H. M., J. Am. Chem. Soc. 1964, 86, 940-941. 9. Safavi, A.; Karimi, M. A., Anal. Chim. Acta 2002, 468(1), 53-63. 10. Fletcher, P.; Andrew, K. N.; Calokerinos, A. C.; Forbes, S.; Worsfold, P. J., Luminescence 2001, 16(1), 1-23. 11. 葉慧君, 國立台灣大學博士論文 2001. 12. Saenger, W., Angew. Chem.-Int. Edit. Engl. 1980, 19(5), 344-362. 13. 吳斯燻; 丁望閒, 化學 2004, 62(4), 517-532. 14. Connors, K. A., Chem. Rev. 1997, 97(5), 1325-1357. 15. Saenger, W., Biochem. Soc. Trans. 1983, 11(2), 136-139. 16. Szejtli, J., Chem. Rev. 1998, 98(5), 1743-1753. 17. 謝茜如; 魏國佐, 化學 2001, 59(3), 409-422. 18. Ribeiro, L.; Ferreira, D. C.; Veiga, F. J. B., J. Control. Release 2005, 103(2), 325-339. 19. Uekama, K., Yakugaku Zasshi-J. Pharm. Soc. Jpn. 2004, 124(12), 909-935. 20. Yanez, C.; Salazar, R.; Nunez-Vergara, L. J.; Squella, J. A., J. Pharm. Biomed. Anal. 2004, 35(1), 51-56. 21. Satterfield, M.; Brodbelt, J. S., Anal. Chem. 2000, 72(24), 5898-5906. 22. Bravo, L., Nutr. Rev. 1998, 56(11), 317-333. 23. Cai, Q. Y.; Rahn, R. O.; Zhang, R. W., Cancer Lett. 1997, 119(1), 99-107. 24. Santos-Buelga, C.; Scalbert, A., J. Sci. Food Agric. 2000, 80(7), 1094-1117. 25. 許夏芬; 張肇麟; 朱燕華, 台灣農業化學與食品科學 2000, 38(5), 377-387. 26. Burda, S.; Oleszek, W., J. Agric. Food Chem. 2001, 49(6), 2774-2779. 27. 晏文潔; 李家璞; 杜平直, 台灣農業化學與食品科學 2000, 38(1), 80-88. 28. Lin, J.-M.; Yamada, M., Anal. Chem. 1999, 71, 1760-1766. 29. Evmiridis, N. P., Analyst 1988, 113(7), 1051-1056. 30. Zhang, G. F.; Chen, H. Y., Anal. Chim. Acta 2000, 409(1-2), 75-81. 31. Lin, J. M.; Arakawa, H.; Yamada, M., Anal. Chim. Acta 1998, 371(2-3), 171-176. 32. Lin, Q.; Guiraum, A.; Escobar, R., Anal. Chim. Acta 1993, 283, 379-385. 33. Li, Y.; Tang, Y.; Yao, H.; Fu, J., Luminescence 2003, 18, 313-317. 34. Zhou, Y.; Nagaoka, T.; Li, F.; Zhu, G., Talanta 1999, 48, 461-467. 35. 李以仁; 鄭博元, 清華大學物理化學實驗手冊-Studies of Fast Reaction Kinetics with the Stopped-Flow Method 2003. 36. Woolf, E. J.; Grayeski, M. L., J. Lumines. 1987, 39(1), 19-27. 37. Yuan, D.-Q.; Lu, J.; Atsumi, M.; Izuka, A.; Kai, M.; Fujita, K., Chem Commun. 2002, (7), 730-731. 38. Hinze, W. L.; Riehl, T. E.; Singh, H. N.; Baba, Y., Anal. Chem. 1984, 56(12), 2180-2191. 39. Vasilescu, M.; Constantinescu, T.; Voicescu, M.; Lemmetyinen, H.; Vuorimaa, E., J. Fluoresc. 2003, 13(4), 315-322. 40. Cao, Z.; Lau, C.; Lu, J., Analyst 2004, 129, 1262-1266. 41. Stauff, J.; Schmidkunz, H.; Hartmann, G., Nature 1963, 198, 281. 42. 蕭寧馨, 董氏基金會食品營養組-營養教育資訊網-油溶性維生素 2004. 43. Madhavi, D. L.; Deshpande, S. S.; Salunkhe, D. K., Food antioxidants: technological, toxicological, and health perspectives. New York: Marcel Dekker: 1996. 44. Vora, J.; Boroujerdi, M., Drug Dev. Ind. Pharm. 1995, 21(4), 495-502. 45. Hirayama, O.; Takagi, M.; Hukumoto, K.; Katoh, S., Anal. Biochem. 1997, 247(2), 237-241. 46. Wei, Y. L.; Ding, L. H.; Dong, C.; Niu, W. P.; Shuang, S. M., Spectroc. Acta Pt. A-Molec. Biomolec. Spectr. 2003, 59(12), 2697-2703. 47. Dotsikas, Y.; Kontopanou, E.; Allagiannis, C.; Loukas, Y. L., J. Pharm. Biomed. Anal. 2000, 23(6), 997-1003. 48. Nishijo, J.; Nagai, M.; Yasuda, M.; Ohno, E.; Ushiroda, Y., J. Pharm. Sci. 1995, 84(12), 1420-1426. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/38525 | - |
dc.description.abstract | 本研究主要是利用停止流儀(Stopped-flow spectrometry)發展以β-環糊精(β-cyclodextrin)增強過碘酸鉀(KIO4)氧化luminol的化學發光系統。探討影響化學發光的因素,包括pH值、反應物(luminol、過碘酸鉀和環糊精)濃度等等,找出最佳化條件。實驗中亦發現pH值的控制對於訊號再現性的影響非常重要。於是選擇在pH 12.0的強鹼溶液下,添加5 mM β-環糊精增強0.05 mM luminol和0.2 mM過碘酸鉀的化學發光強度約190倍左右。若比較不同腔體大小的α-或γ-環糊精,則增強效果比β-環糊精降低約6倍左右,推測是形成可以穩定化學發光中間態的β-環糊精包絡物所致,因此利用檢測物與化學發光中間態彼此競爭形成β-環糊精包絡物而抑制訊號的機制,可應用於檢測抗氧化劑,例如:trolox、BHA、類黃酮(flavonoids)等。其中,trolox在1.5 x 10-8∼2.5 x 10-7 M內以指數形式呈現快速且明顯的抑制效果,對於抑制化學發光訊號強度一半之濃度(IC50值)達0.05 μM;另外,亦可區分類黃酮的抗氧化能力在不同的氫氧取代基位置,具有不同的抑制效果。最後,此系統亦嘗試應用於非抗氧化劑的檢測,如螢光標記物-TNS,得到IC50值為19.9 μM,推論TNS和luminol共同競爭β-環糊精的疏水性腔體導致訊號降低。未來,將持續對於系統的機制作更進一步的探討,以及增加其應用性。 | zh_TW |
dc.description.abstract | In this study, a novel chemiluminescence (CL) method based on the luminol-KIO4-β-cyclodextrin(β-CD) system has been developed. The stopped-flow spectrometry was used to measure the CL produced from the oxidation of luminol by KIO4 in alkaline condition (pH 12.0). Various factors, including pH, concentration of reagents (luminol, KIO4, and β-CD), and temperature, that affect the CL intensity were also discussed. The results indicate that maintaining a constant pH is very important for the reproducibility of the CL signal. In addition, the CL intensity was enhanced about 190-fold by the presence of 5 mM β-CD in 0.05 mM luminol and 0.2 mM KIO4. α– and γ–CD caused much weaker enhancement (about 1/6) as compared to β-CD, suggesting that cavity of correct size was required. It’s proposed that the enhancement is due to the stabilization of CL intermediate by the formation of β-CD inclusion complex. Therefore, the inhibition of CL intensity by the competition between analytes and luminol intermediate to form the β-CD inclusion complex was employed to determine the antioxidants, such as trolox, butylated hydroxyanisole (BHA), and flavonoids. The trolox quenched the CL intensity exponentially in the range of 1.5×10-8~2.5×10-7M, and the half-inhibition concentration (IC50) was 0.05 μM. On the other hand, the antioxidant activities of different flavonoids can be distinguished by their abilities in CL inhibition. Furthermore, the CL method was also applied to determine a non-antioxidant, such as fluorescence probe-TNS, and the IC50 value was 19.9 μM. In the future, we will try to explain the mechanism of the CL system and to develop more applications. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T16:36:15Z (GMT). No. of bitstreams: 1 ntu-94-R92223029-1.pdf: 2124808 bytes, checksum: a27f0a9cf66b95e3078d9dd7782002e0 (MD5) Previous issue date: 2005 | en |
dc.description.tableofcontents | 中文摘要..............................................I
英文摘要.............................................II 目 錄........................................... III 圖 目 錄.............................................VI 表 目 錄.............................................XI 第一章 緒論 第一節 化學發光之簡介...........................1 第二節 環糊精的結構與性質.......................5 第三節 類黃酮的介紹.............................9 第四節 以IO4-為氧化劑之化學發系統介紹.........13 第五節 研究動機................................14 第二章 實驗部分 第一節 實驗藥品................................15 第二節 溶液的配製..............................16 第三節 實驗儀器................................17 第四節 實驗方法................................19 2.4.1 停止流光譜儀的原理....................19 2.4.2 化學發光之偵測........................22 2.4.3 形成常數之偵測........................24 第三章 結果與討論 第一節 Luminol-KIO4-β-CD之化學發光系統.........25 3.1.1 簡 介..................................25 3.1.2 β-環糊精對化學發光強度的影響............25 3.1.3 pH值對化學發光的影響....................28 3.1.4 KIO4和luminol濃度對化學發光強度的影響...32 3.1.5 環糊精種類對化學發光強度及效應的影響....35 3.1.6 溫度對化學發光的影響....................37 3.1.7 發光光譜的量測..........................39 3.1.8 luminol-KIO4-β-CD系統發光機制之探討.....40 第二節 luminol-KIO4-β-CD系統之應用................43 3.2.1 抗氧化劑之偵測-簡 介...................43 3.2.2 抗氧化劑之偵測-trolox..................46 3.2.3 抗氧化劑之偵測-BHA.....................49 3.2.4 抗氧化劑之偵測-類黃酮..................51 3.2.5 抗氧化能力的比較-IC50值................64 3.2.6 檢測物與β-環糊精的形成常數..............67 3.2.7 非抗氧化劑之偵測-TNS...................77 第四章 結論與未來展望............................82 第五章 參考文獻..................................84 | |
dc.language.iso | zh-TW | |
dc.title | β-環糊精增強過碘酸鉀氧化Luminol之化學發光及應用 | zh_TW |
dc.title | Enhancement of Chemiluminescence by β-Cyclodextrin for the Oxidation of Luminol with Potassium Periodate and Its Applications | en |
dc.type | Thesis | |
dc.date.schoolyear | 93-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 何東英,周禮君 | |
dc.subject.keyword | 化學發光,過碘酸鉀,β-環糊精,類黃酮, | zh_TW |
dc.subject.keyword | Chemiluminescence,Potassium Periodate,β-Cyclodextrin,Flavonoids, | en |
dc.relation.page | 86 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2005-07-07 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 化學研究所 | zh_TW |
顯示於系所單位: | 化學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-94-1.pdf 目前未授權公開取用 | 2.08 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。