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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 曾顯雄 | |
dc.contributor.author | Hung-Yi Wu | en |
dc.contributor.author | 吳竑毅 | zh_TW |
dc.date.accessioned | 2021-06-13T16:30:04Z | - |
dc.date.available | 2010-07-19 | |
dc.date.copyright | 2005-07-19 | |
dc.date.issued | 2005 | |
dc.date.submitted | 2005-07-12 | |
dc.identifier.citation | 王伯徹、陳啟楨,1994。靈芝。台灣常見食藥用菇類介紹。財團法人食品工業發展研究所。新竹。台灣。
王伯徹、陳啟楨、華傑。1998。食藥用菇類的栽培與應用。財團法人食品工業發展研究所。新竹。台灣。 Adaskaveg, J. E. and Gilbertson, R. L. 1986. Vegetative incompatibility between intraspecific dikaryotic pairings of Ganoderma lucidum and Ganoderma tsugae. Mycologia 603. Arima, T., Yamamoto, M., Hirata, A., Kawano, S., and Kamada, T. 2004. The eln3 gene involved in fruiting body morphogenesis of Coprinus cinereus encodes a putative membrane protein with a general glycosyltransferase domain. Fungal. Genet Biol. 41: 805−812. Badrane, H. and May, G. 1999. The divergence−homogenization duality in the evolution of the b1 mating type gene of Coprinus cinereus. Mol. Biol. Evol. 16: 975−986. Banerjee, S. and Sarker, A. 1958. Studies on heterothallism−IV. Ganoderma lucidum (Leyss) Karst. Proc. Indian Acad. Sci. 49: 94−98. Banham, A. H., Asante−Owusu, R. N., Gottgens, B., Thompson, S., Kingsnorth, C. S., Mellor, E., and Casselton, L. A. 1995. An N−terminal dimerization domain permits homeodomain proteins to choose compatible partners and initiate sexual development in the Mushroom Coprinus cinereus. Plant Cell 7: 773−783. Bardwell, L. 2005. A walk−through of the yeast mating pheromone response pathway. Peptides 26: 339−350. Bardwell, L., Cook, J. G., Inouye, C. J., and Thorner, J. 1994. Signal propagation and regulation in the mating pheromone response pathway of the yeast Saccharomyces cerevisiae. Dev. Biol. 166: 363−379. Baxevanis, A. D. and Vinson, C. R. 1993. Interactions of coiled coils in transcription factors: where is the specificity? Curr. Opin. Genet Dev. 3: 278−285. Bourne, H. R. 1997. How receptors talk to trimeric G proteins. Curr. Opin. Cell Biol. 9: 134−142. Brown, A. J. and Casselton, L. A. 2001. Mating in mushrooms: increasing the chances but prolonging the affair. Trends Genet 17: 393−400. Burglin, T. R. 1994. A comprehensive classification og homeobox genes, p.25−72. In D. Duboule(ed), Gudiebook to the homeobox genes. Oxford University Press, Oxford, United Kingdom. Burglin, T. R. 1993. A comprehensive survey of homeodomain sequence and associated motifs. In Deboule D(ed) A guidebook for homeobox genes. Oxford University Press, Oxford (in press) Caldwell, G. A., Naider, F., and Becker, J. M. 1995. Fungal lipopeptide mating pheromones: a model system for the study of protein prenylation. Microbiol. Rev. 59: 406−422. Casselton, L. A. and Olesnicky, N. S. 1998. Molecular genetics of mating recognition in basidiomycete fungi. Microbiol. Mol. Biol. Rev. 62: 55−70. Celerin, M., Merino, S. T., Stone, J. E., Menzie, A. M., and Zolan, M. E. 2000. Multiple roles of Spo11 in meiotic chromosome behavior. EMBO J. 19: 2739−2750. Chang, T. T. and Chen, T. 1986. Studies on nuclear behavior mating type and heterokaryosis of several species of Ganoderma in Taiwan. Plant Prot. Bull 28: 231−240. Cheung, W. M., Hui, W. S., Chu, P. W., Chiu, S. W., and Ip, N. Y. 2000. Ganoderma extract activates MAP kinases and induces the neuronal differentiation of rat pheochromocytoma PC12 cells. FEBS Lett. 486: 291-296. Cohen, C. and Parry, D. A. D. 1990. Proteins: structure, function and genetics. α−helical coiled coils and bundles: how to design an α−helical protein. Protein 7: 1−15. Chomczynski, P. and Sacchi, N. 1987. Single step method of RNA isolation by acid guanidinium thiocyanate - phenol - chloroform extraction. Anal. Biochem. 162: 156. Cummings, W. J., Merino, S. T., Young, K. G., Li, L., Johnson, C. W., Sierra, E. A., and Zolan, M. E. 2002. The Coprinus cinereus adherin Rad9 functions in Mre11−dependent DNA repair, meiotic sister−chromatid cohesion, and meiotic homolog pairing. Proc. Natl. Acad. Sci. U. S. A. 99: 14958−14963. Day, P. R. 1963. Mutations affecting the A mating−type locus in Coprinus lagopus. Genet. Res. Camb 4: 323−325. De Groot, M. J., Bundock, P., Hooykaas, P. J., and Beijersbergen, A. G. 1998. Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat. Biotechnol. 16: 839-842. De Groot, P. W., Schaap, P. J., Van Griensven, L. J., and Visser, J. 1997. Isolation of developmentally regulated genes from the edible mushroom Agaricus bisporus. Microbiology 143: 1993−2001. Diatchenko, L., Lau, Y. F., Campbell, A. P., Chenchik, A., Moqadam, F., Huang, B., Lukyanov, S., Lukyanov, K., Gurskaya, N., Sverdlov, E. D., and Siebert, P. D. 1996. Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc. Natl. Acad. Sci. U. S. A. 93: 6025-6030. Dohlman, H. G. 2002. G proteins and pheromone signaling. Annu. Rev. Physiol 64: 129−152. Dohlman, H. G., Thorner, J., Caron, M. G., and Lefkowitz, R. J. 1991. Model systems for the study of seven−transmembrane−segment receptors. Annu. Rev. Biochem. 60: 653−688. Dohlman, H. G. and Thorner, J. W. 2001. Regulation of G protein−initiated signal transduction in yeast: paradigms and principles. Annu. Rev. Biochem. 70: 703−754. Doyle, J. J. and Doyle, J. L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 11−15. Doyle, J. J. and Doyle, J. L. 1990. A rapid total DNA preparation procedure for fresh plant tissue. Focus 12: 13−15. El-Mekkawy, S., Meselhy, M. R., Nakamura, N., Tezuka, Y., Hattori, M., Kakiuchi, N., Shimotohno, K., Kawahata, T., and Otake, T. 1998. Anti-HIV-1 and anti-HIV-1-protease substances from Ganoderma lucidum. Phytochemistry 49: 1651-1657. Elion, E. A. 2000. Pheromone response, mating and cell biology. Curr. Opin. Microbiol. 3: 573−581. Eo, S. K., Kim, Y. S., Lee, C. K., and Han, S. S. 2000. Possible mode of antiviral activity of acidic protein bound polysaccharide isolated from Ganoderma lucidum on herpes simplex viruses. J Ethnopharmacol. 72: 475-481. Fernandez Espinar, M. T. and Labarere, J. 1997. Cloning and sequencing of the Aa−Pri1 gene specifically expressed during fruiting initiation in the edible mushroom Agrocybe aegerita, and analysis of the predicted amino−acid sequence. Curr. Genet 32: 420−424. Fowler, T. J., Mitton, M. F., Rees, E. I., and Raper, C. A. 2004. Crossing the boundary between the Bα and Bβ mating−type loci in Schizophyllum commune. Fungal. Genet Biol. 41: 89−101. Fowler, T. J., Mitton, M. F., Vaillancourt, L. J., and Raper, C. A. 2001. Changes in mate recognition through alterations of pheromones and receptors in the multisexual mushroom fungus Schizophyllum commune. Genetics 158: 1491−1503. Gan, K. H., Fann, Y. F., Hsu, S. H., Kuo, K. W., and Lin, C. N. 1998. Mediation of the cytotoxicity of lanostanoids and steroids of Ganoderma tsugae through apoptosis and cell cycle. J Nat. Prod. 61: 485-487. Gehring, W. J., Qian, Y. Q., Billeter, M., Furukubo−Tokunaga, K., Schier, A. F., Resendez−Perez, D., Affolter, M., Otting, G., and Wuthrich, K. 1994. Homeodomain−DNA recognition. Cell 78: 211−223. Gerecke, E. E. and Zolan, M. E. 2000. An mre11 mutant of Coprinus cinereus has defects in meiotic chromosome pairing, condensation and synapsis. Genetics 154: 1125−1139. Giasson, L., Specht, C. A., Milgrim, C., Novotny, C. P., and Ullrich, R. C. 1989. Cloning and comparison of A alpha mating−type alleles of the Basidiomycete Schizophyllum commune. Mol. Gen. Genet. 218: 72−77. Gieser, P. T. and May, G. 1994. Comparison of two b1 alleles from within the A mating−type of the basidiomycete Coprinus cinereus. Gene 146: 167−176. Giesy, R. M. and Day, P. R. 1965. The septal pores of Coprinus lagopus (Fr.) sensu Buller in relation to nuclear migration. Am. J. Bot. 52: 287−294. Gustin, M. C., Albertyn, J., Alexander, M., and Davenport, K. 1998. MAP kinase pathways in the yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62: 1264−1300. Halsall, J. R., Milner, M. J., and Casselton, L. A. 2000a. Three subfamilies of pheromone and receptor genes generate multiple B mating specificities in the mushroom Coprinus cinereus. Genetics 154: 1115−1123. Hamer, J. E. and Timberlake, W. E. 1987. Functional organization of the Aspergillus nidulans trpC promoter. Mol. Cell Biol. 7: 2352−2359. Hargrave, P. A. and McDowell, J. H. 1992. Rhodopsin and phototransduction: a model system for G protein−linked receptors. FASEB J. 6: 2323−2331. Haylock, R. W., Economou, A., and Casselton, L. A. 1980. Dikaryon formation in Coprinus cinereus: selection and identification of B factor mutants. J. Gen. Microbiol. 121: 17−26. Herskowitz, I. 1995. MAP kinase pathways in yeast: for mating and more. Cell 80: 187−197. Horton, J. S., Palmer, G. E., and Smith, W. J. 1999. Regulation of dikaryon−expressed genes by FRT1 in the basidiomycete Schizophyllum commune. Fungal. Genet Biol. 26: 33−47. Horton, J. S. and Raper, C. A. 1991. A mushroom−inducing DNA sequence isolated from the Basidiomycete, Schizophyllum commune. Genetics 129: 707−716. Horton, J. S. and Raper, C. A. 1995. The mushroom−inducing gene Frt1 of Schizophyllum commune encodes a putative nucleotide−binding protein. Mol. Gen. Genet 247: 358−366. Inada, K., Morimoto, Y., Arima, T., Murata, Y., and Kamada, T. 2001. The clp1 gene of the mushroom Coprinus cinereus is essential for A−regulated sexual development. Genetics 157: 133−140. James, T. Y., Kues, U., Rehner, S. A., and Vilgalys, R. 2004a. Evolution of the gene encoding mitochondrial intermediate peptidase and its cosegregation with the A mating−type locus of mushroom fungi. Fungal. Genet Biol. 41: 381−390. James, T. Y., Liou, S. R., and Vilgalys, R. 2004b. The genetic structure and diversity of the A and B mating−type genes from the tropical oyster mushroom, Pleurotus djamor. Fungal. Genet Biol. 41: 813−825. Jarvis, W. D., Turner, A. J., Povirk, L. F., Traylor, R. S., and Grant, S. 1994. Induction of apoptotic DNA fragmentation and cell death in HL-60 human promyelocytic leukemia cells by pharmacological inhibitors of protein kinase C. Cancer Res 54: 1707- 1714. Jeansonne, N. E. 1994. Yeast as a model system for mammalian seven−transmembrane segment receptors. Proc. Soc. Exp. Biol. Med. 206: 35−44. Jersild, R., Mishkin, S., and Niederpruem, D. 1967. Origin and ultrastructure of complex septa in Schizophyllum commune development. Arch. Microbiol. 57: 20−32. Jong, S. C. and Birmingham, J. M., 1992. Medicinal benefits of the mushroom Ganoderma. Advances in Applied Microbiology 37: 101–134. Kajiwara, S., Yamaoka, K., Hori, K., Miyazawa, H., Saito, T., Kanno, T., and Shishido, K. 1992. Isolation and sequence of a developmentally regulated putative novel gene, priA, from the basidiomycete Lentinus edodes. Gene 114: 173−178. Kamper, J., Reichmann, M., Romeis, T., Bolker, M., and Kahmann, R. 1995. Multiallelic recognition: nonself−dependent dimerization of the bE and bW homeodomain proteins in Ustilago maydis. Cell 81: 73−83. Koltin, Y. 1968. The genetics structure of the incompatibility factors of Schizophyllum commune. Comparative studies of primary mutations in the B factor. Mol. Gen. Genet. 102: 196−303. Koltin, Y. and Flexer, A. S. 1969. Alteration of nuclear migration in B−mutant strains of Schizophyllum commune. J. Cell Sci. 4: 739−749. Koltin, Y., Raper, J. R., and Simchen, G. 1967. The genetic structure of the incompatibility factors of Schizophyllum commune. The B factor. Proc. Natl. Acad. Sci. U. S. A. 57: 55−62. Koltin, Y., Stamberg, J., Bawnik, N., Tamaekin, R., and Werczberger, R. 1979. Mutational analysis of natural alleles in and affecting the B incompatibility factor of Schizophyllum. Genetics 93: 383−391. Kothe, E. 2001. Mating−type genes for basidiomycete strain improvement in mushroom farming. Appl. Microbiol. Biotechnol. 56: 602−612. Kothe, E. 1999. Mating types and pheromone recognition in the Homobasidiomycete Schizophyllum commune. Fungal. Genet Biol. 27: 146−152. Kronstad, J. W. and Leong, S. A. 1990. The b mating−type locus of Ustilago maydis contains variable and constant regions. Genes Dev. 4: 1384−1395. Kuchler, K., Sterne, R. E., and Thorner, J. 1989. Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells. EMBO J. 8: 3973−3984. Kues, U. 2000. Life history and developmental processes in the basidiomycete Coprinus cinereus. Microbiol. Mol. Biol. Rev. 64: 316−353. Kues, U. and Casselton, L. A. 1992. Homeodomains and regulation of sexual development in basidiomycetes. Trends Genet. 8: 154−155. Kues, U. and Casselton, L. A. 1993. The origin of multiple mating−types in mushrooms. J. Cell Sci. 104: 227−230. Kues, U., Gottgens, B., Stratmann, R., Richardson, W. V., O'Shea, S. F., and Casselton, L. A. 1994a. A chimeric homeodomain protein causes self−compatibility and constitutive sexual development in the mushroom Coprinus cinereus. EMBO J. 13: 4054−4059. Kues, U., Richardson, W. V., Tymon, A. M., Mutasa, E. S., Gottgens, B., Gaubatx, S., Gregoriades, A., and Casselton, L. A. 1992. The combination of dissimilar alleles of the Aα and Aβ gene complexes, whose proteins contain homeodomain motifs, determines sexual development in the mushroom Coprinus cinereus. Genes Dev. 6: 568−577. Kues, U., sante−Owusu, R. N., Mutasa, E. S., Tymon, A. M., Pardo, E. H., O'Shea, S. F., Gottgens, B., and Casselton, L. A. 1994b. Two classes of homeodomain proteins specify the multiple a mating types of the mushroom Coprinus cinereus. Plant Cell 6: 1467−1475. Kues, U., Tymon, A. M., Richardson, W. V., May, G., Gieser, P. T., and Casselton, L. A. 1994c. A mating−type factors of Coprinus cinereus have variable numbers of specificity genes encoding two classes of homeodomain proteins. Mol. Gen. Genet. 245: 45−52. Lengeler, K. B., Davidson, R. C., D'souza, C., Harashima, T., Shen, W. C., Wang, P., Pan, X., Waugh, M., and Heitman, J. 2000. Signal transduction cAscades regulating fungal development and virulence. Microbiol. Mol. Biol. Rev. 64: 746−785. Lengeler, K. B. and Kothe, E. 1999b. Mated: a putative peptide transporter of Schizophyllum commune expressed in dikaryons. Curr. Genet 36: 159−164. Lengeler, K. B. and Kothe, E. 1999a. Identification and characterization of brt1, a gene down−regulated during B−regulated development in Schizophyllum commune. Curr. Genet 35: 551−556. Lieu, C. W., Lee, S. S., and Wang, S. Y. 1992. The effect of Ganoderma lucidum on induction of differentiation in leukemic U937 cells. Anticancer Res 12: 1211-1215. Lupas, A. 1996. Coiled coils: new structures and new functions. Trends Biochem. Sci. 21: 375−382. Magae, Y., Novotny, C., and Ullrich, R. 1995. Interaction of the A alpha Y and Z mating−type homeodomain proteins of Schizophyllum commune detected by the two−hybrid system. Biochem. Biophys. Res. Commun. 211: 1071−1076. Marcus, S., Polverino, A., Barr, M., and Wigler, M. 1994. Complexes between STE5 and components of the pheromone−responsive mitogen−activated protein kinase module. Proc. Natl. Acad. Sci. U. S. A. 91: 7762−7766. McGrath, J. P. and Varshavsky, A. 1989. The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P−glycoprotein. Nature 340: 400−404. Merino, S. T., Cummings, W. J., Acharya, S. N., and Zolan, M. E. 2000. Replication−dependent early meiotic requirement for Spo11 and Rad50. Proc. Natl. Acad. Sci. U. S. A. 97: 10477−10482. Miyazaki, Y., Nakamura, M., and Babasaki, K. 2005. Molecular cloning of developmentally specific genes by representational difference analysis during the fruiting body formation in the basidiomycete Lentinula edodes. Fungal. Genet Biol. 42: 493−505. Miyazaki, Y., Sakuragi, Y., Yamazaki, T., and Shishido, K. 2004. Target genes of the developmental regulator PRIB of the mushroom Lentinula edodes. Biosci. Biotechnol. Biochem. 68: 1898−1905. Miyazaki, Y., Tsunoka, O., and Shishido, K. 1997. Determination of the DNA−binding sequences of the Zn(II)2Cys6 zinc−cluster−containing PRIB protein, derived from the basidiomycete Lentinus edodes gene. J. Biochem. (Tokyo) 122: 1088−1091. Mullaney, E. J., Hamer, J. E., Roberti, K. A., Yelton, M. M., and Timberlake, W. E. 1985. Primary structure of the trpC gene from Aspergillus nidulans. Mol. Gen Genet 199: 37−45. Muraguchi, H. and Kamada, T. 1998. The ich1 gene of the mushroom Coprinus cinereus is essential for pileus formation in fruiting. Development 125: 3133−3141. Muraguchi, H. and Kamada, T. 2000. A mutation in the eln2 gene encoding a cytochrome P450 of Coprinus cinereus affects mushroom morphogenesis. Fungal. Genet Biol. 29: 49−59. Murata, Y., Fujii, M., Zolan, M. E., and Kamada, T. 1998. Molecular analysis of pcc1, a gene that leads to A−regulated sexual morphogenesis in Coprinus cinereus. Genetics 149: 1753−1761. Mutasa, E. S., Tymon, A. M., Gottgens, B., Mellon, F. M., Little, P. F. R., and Casselton, L. A. 1990. Molecular organization of an A mating type factor of the basidiomycete fungus Coprinus cinereus. Curr. Genet. 18: 223−229. Naider, F. and Becker, J. M. 2004. The α−factor mating pheromone of Saccharomyces cerevisiae: a model for studying the interaction of peptide hormones and G protein−coupled receptors. Peptides 25: 1441−1463. Nara, T., Saka, T., Sawado, T., Takase, H., Ito, Y., Hotta, Y., and Sakaguchi, K. 1999. Isolation of a LIM15/DMC1 homolog from the basidiomycete Coprinus cinereus and its expression in relation to meiotic chromosome pairing. Mol. Gen Genet 262: 781−789. Novotny, C. P., Stankis, M. M., Specht, C. A., Yang, H., Giasson, L., and Ullrich, R. C. 1991. The Aα mating type locus of Schizophyllum commune. In: Bennett JW, Lasure LL(eds) More gene manipulations in fungi. Academic Press, San Diego, California, pp 235−257 O'Shea, E. K., Rutkowski, R., and Kim, P. S. 1992. Mechanism of specificity in the Fos−Jun oncoprotein heterodimer. Cell 68: 699−708. O'Shea, S. F., Chaure, P. T., Halsall, J. R., Olesnicky, N. S., Leibbrandt, A., Connerton, I. F., and Casselton, L. A. 1998. A large pheromone and receptor gene complex determines multiple B mating type specificities in Coprinus cinereus. Genetics 148: 1081−1090. Papazian, H. P. 1950. Physiology of the incompatibility factors in Schizophyllum commune. Bot. Gaz. 112: 143−163. Papazian, H. P. 1951. The incompatibility factors and a related gene in Schizophyllum commune. Genetics 36: 441−459. Paragy, Y. 1962. Mutations in the B incompatibility factor of Schizophyllum commune. Proc. Natl. Acad. Sci. U. S. A. 743−750. Pardo, E. H., O'Shea, S. F., and Casselton, L. A. 1996. Multiple versions of the A mating type locus of Coprinus cinereus are generated by three paralogous pairs of multiallelic homeobox genes. Genetics 144: 87−94. Posas, F., Takekawa, M., and Saito, H. 1998. Signal transduction by MAP kinase cascades in budding yeast. Curr. Opin. Microbiol. 1: 175−182. Punt, P. J., Dingemanse, M. A., Kuyvenhoven, A., Soede, R. D., Pouwels, P. H., and van den Hondel, C. A. 1990. Functional elements in the promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde−3−phosphate dehydrogenase. Gene 93: 101−109. Ramirez, L., Larraya, L. M., and Pisabarro, A. G. 2000. Molecular tools for breeding basidiomycetes. Int. Microbiol. 3: 147−152. Raper, C. A. 1983. Control for development and differentiation in basidiomycetes. In: Bennett JW, Ciegler A(eds)Secondary metabolism and differentiation in fungi. Marcel Dekker, NewYork, pp 195−238 Raper, J. R. 1966. Genetics of sexuality in higher fungi. Ronald Press, New York. Raper, J. R., Baxter, M. G., and Ellingboe, A. H. 1960. The genetic structure of the incompatibility factors of Schizophyllum commune: the A factor. Proc. Natl. Acad. Sci. U. S. A. 46: 833−842. Raper, J. R., Baxter, M. G., and Middleton, R. B. 1958a. The genetics structure of the incompatibility factors in Schizophyllum commune. Proc. Natl. Acad. Sci. U. S. A. 44: 889−900. Raper, J. R., Boyd, D. H., and Raper, J. R. 1965. Primary and secondary mutations at the incompatibility loci in Schizophyllum. Proc. Natl. Acad. Sci. U. S. A. 53: 1324−1332. Raper, J. R., Krongelb, G. S., and Baxter, M. G. 1958b. The number and distribution of incompatibility factors in Schizophyllum commune. Am. Natl. 92: 221−232. Raudaskoski, M., Stamberg, J., Bawnik, N., and Koltin, Y. 1976. Mutational analysis of natural alleles at the B incompatibility factor of Schizophyllum commune. Genetics 83: 507−516. Sakmar, T. P. 2002. Structure of rhodopsin and the superfamily of seven−helical receptors: the same and not the same. Curr. Opin. Cell Biol. 14: 189−195. Schaefer, B. C. 1995. Revolutions in rapid amplification of cDNA ends: new strategies for polymerase chain reaction cloning of full-length cDNA ends. Anal. Biochem. 227: 255-273. Schulz, B., Banuett, B., Dahl, M., Schlesinger, R., Schafer, W., Martin, T., Herskowitz, I., and Kahmann, R. 1990. The b alleles of U. maydis, whose combinations programme pathogenic development, code for polypeptides containing a homeodomain−related motif. Cell 60: 295−306. Scott, M. P., Tamkum, J. W., and Hatzell, G. W. I. 1989. The Structure and function of the homeodomain. Biochim. Biophys. Acta. 989: 25−48. Seitz, L. C., Tang, K., Cummings, W. J., and Zolan, M. E. 1996. The rad9 gene of Coprinus cinereus encodes a proline−rich protein required for meiotic chromosome condensation and synapsis. Genetics 142: 1105−1117. Shen, G. P., Park, D. C., Ullrich, R. C., and Novotny, C. P. 1996. Cloning and characterization of a Schizophyllum gene with A beta 6 mating−type activity. Curr. Genet. 29: 136−142. Shiao, M. S. 2003. Natural products of the medicinal fungus Ganoderma lucidum: occurrence, biological activities, and pharmacological functions. The Chem. Rec. 3: 172-180. Shiao, M-S., K. R. Lee, L.J. Lin & C. T. Wang. 1994. Natural products and biological activities of the Chinese medical fungus, Ganoderma lucidum. In Food Phytochemicals for Cancer Prevention. II: Teas, Spices, and Herbs. C.T. Ho, T. Osawa, M.T. Huang & R.T. Rosen, Eds.: 342-354. American Chemical Society. Washington, DC. Specht, C. A. 1995. Isolation of the B alpha and B beta mating−type loci of Schizophyllum commune. Curr. Genet 28: 374−379. Specht, C. A., Stankis, M. M., Giasson, L., Novotny, C. P., and Ullrich, R. C. 1992. Functional analysis of the homeodomain−related protein of the Aα locus of Schizophyllum commune. Proc. Natl. Acad. Sci. U. S. A. 89: 7174−7178. Specht, C. A., Stankis, M. M., Novotny, C. P., and Ullrich, R. C. 1994. Mapping the heterogeneous DNA region that determines the nine A alpha mating−type specificities of Schizophyllum commune. Genetics 137: 709−714. Stankis, M. M., Specht, C. A., Yang, H., Giasson, L., Ullrich, R. C., and Novotny, C. P. 1992. The A alpha mating locus of Schizophyllum commune encodes two dissimilar multiallelic homeodomain proteins. Proc. Natl. Acad. Sci. U. S. A. 89: 7169−7173. Swamy, S., Uno, I., and Ishikawa, T. 1984. Morphogenetic effects of mutations at the A and B incompatibility factors in Coprinus cinereus. J. Gen. Microbiol. 130: 3219−3224. Swiezynski, K. M. and Day, P. R. 1960. Heterokaryon formation in Coprinus cinereus. Genet. Res. Camb 1: 114−128. Tymon, A. M., Kues, U., Richardson, W. V., and Casselton, L. A. 1992. A fungal mating type protein that regulates sexual and asexual development contains a POU−related domain. EMBO J. 11: 1805−1813. Ullrich, R. C., Specht, C. A., Stankis, M. M., Yang, H., Giasson, L., and Novotny, C. P. 1991. Molecular biology of mating−type determination in Schizophyllum commune. In: Setlow JK (ed) Genetic engineering, principles and methods, vol. 13. Plenum Press, New York, pp 279−306 van Wetter, M. A., Wosten, H. A., and Wessels, J. G. 2000. SC3 and SC4 hydrophobins have distinct roles in formation of aerial structures in dikaryons of Schizophyllum commune. Mol. Microbiol. 36: 201−210. Vogelstein, B. and Gillespie, D. 1979. Preparative and analytical purification of DNA from agarose. Proc. Natl. Acad. Sci. U. S. A. 76: 615-619. Wang, S. Y., Hsu, M. L., Hsu, H. C., Tzeng, C. H., Lee, S. S., Shiao, M. S., and Ho, C. K. 1997. The anti-tumor effect of Ganoderma lucidum is mediated by cytokines released from activated macrophages and T lymphocytes. Int. J Cancer 70: 699-705. Wendland, J., Vaillancourt, L. J., Hegner, J., Lengeler, K. B., Laddison, K. J., Specht, C. A., Raper, C. A., and Kothe, E. 1995. The mating−type locus B alpha 1 of Schizophyllum commune contains a pheromone receptor gene and putative pheromone genes. EMBO J. 14: 5271−5278. Woese, C. R. 1998. Default taxonomy: Ernst Mayr's view of the microbial world. Proc. Natl. Acad. Sci. U. S. A. 95: 11043−11046. Woese, C. R. and Fox, G. E. 1977. Phylogenetic Structure of the Prokaryotic Domain: The Primary Kingdoms. Proc. Natl. Acad. Sci. U. S. A. 74: 5088−5090. Yee, A. R. and Kronstad, J. W. 1993. Construction of chimeric alleles with altered specificity at the b incompatibility locus of Ustilago maydis. Proc. Natl. Acad. Sci. U. S. A. 90: 664−668. Yoon, S. Y., Eo, S. K., Kim, Y. S., Lee, C. K., and Han, S. S. 1994. Antimicrobial activity of Ganoderma lucidum extract alone and in combination with some antibiotics. Arch. Pharm. Res 17: 438-442. Yue, C., Osier, M., Novotny, C. P., and Ullrich, R. C. 1997a. The specificity determinant of the Y mating−type proteins of Schizophyllum commune is also essential for Y−Z protein binding. Genetics 145: 253−260. Zhao, J. and Kwan, H. S. 1999. Characterization, molecular cloning, and differential expression analysis of laccase genes from the edible mushroom Lentinula edodes. Appl. Environ. Microbiol. 65: 4908−4913. Zolan, M. E., Crittenden, J. R., Heyler, N. K., and Seitz, L. C. 1992. Efficient isolation and mapping of rad genes of the fungus Coprinus cinereus using chromosome−specific libraries. Nucleic Acids Res. 20: 3993−3999. Zolan, M. E., Tremel, C. J., and ukkila, P. J. 1988. Production and characterization of radiation−sensitive meiotic mutants of Coprinus cinereus. Genetics 120: 379−387. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/38308 | - |
dc.description.abstract | 靈芝為一種極具價值的藥用真菌,曾被報導具多種抗癌與增進免疫的功能;過去在靈芝交配型與出菇有過基礎生物學之研究,但在分子生物學層次之深入探討則尚付闕如。為瞭解靈芝調控交配型與出菇之基因,首先利用已建構之靈芝基因體與表現序列標記(Expressed Sequence Tag)資料庫,藉由比對與分析,找出可能位於靈芝A mating type locus 之4個交配型基因,以及可能位於靈芝B mating type locus的19個pheromone receptor與20個putative pheromone precursor相關基因,並組合出長約15 kbp的靈芝A mating type locus contig,再藉由RACE (Rapid amplification of cDNA ends)選殖出位於A locus之a1與a2 mating type基因,其中a1 cDNA全長1428 bp,與Pleurotus djamor 之HD1 homeodomain mating type protein具有48%之相似度,並具有與mating type protein 結合與辨識之domain,以及被預測出可能的NLS (Nuclear Localization signal): RKRRR;a2 cDNA全長1680 bp,與P. djamor 之HD2 homeodomain mating type protein具有39%之相似度,並具有保守之homeobox domain,以及可能的NLS:RRSRCRKE。並藉由基因專一性引子對,偵測出交配型A1具有a1與a2基因,A2具有b1與b2基因,B1具有PR3、PR5、PR15與PR19基因,B2具有PR3、PR7與PR8基因。並根據不同單核體交配型之交配試驗,經由細胞學與發生學之鏡檢與觀察,證實並探討不同交配基因調控交配型所獲之結論。為證實a1與a2的功能,已完成建構以靈芝glyceraldehyde - 3 - phosphate dehydrogenase (gpd)基因之promoter驅動下游抗抗生素基因與a1或a2基因表現之binary vector:pCGl-a1與pCGl-a2,並將藉由農桿菌轉型法(Agrobacterium - mediated - transformation)於不同之交配型內表現;並且為了組合出更完整的A與B locus,分別以b1與PR7基因片段做為探針,篩檢已建構完成之Fosmid library,已獲得呈正反應訊號,於A mating type locus有8個選殖株,B mating type locus有5個選殖株,將再進行雜合加以驗證,並做Fosmid兩端定序,與靈芝基因體相關資料庫比對,以建構完整之A、B基因座之基因圖譜(gene map)。另一方面,建構靈芝成熟子實體與菌絲體之雙向扣除式cDNA資料庫(subtractive cDNA library),並配合於靈芝基因體與EST資料庫比對分析,初步結果發現一些可能與靈芝子實體型態分化相關之基因,未來並擬利用基因剔除(gene knockout)之方法來分析個別基因之功能。 | zh_TW |
dc.description.abstract | Ganoderma lucidum is a well-known and important medicinal mushroom demonstrated with anti-cancer and immunomodulatory activities. Although classic study on the mating and fruiting in G. lucidum have been carried out in recent decades and accumulated tremendous valuable knowledge, in-depth exploration of the biologically interesting phenomenon via molecular biology approaches was unavailable and deserved further study. In order to understand the genes in G. lucidum which regulated the mating and fruiting, first we BLAST the Expressed Sequence Tag (EST) from G. lucidum cDNA library, and accessed 4 mating type genes in A mating type locus, and 20 pheromone receptor genes and 19 pheromone precursor genes which controlled mating in B mating locus. A 15 kbp A mating type locus was assembled by linking contigs in whole genomic DNA library of G. lucidum. Full-length open reading frames of genes, a1, a2, encoded homeodomain protein HD1 and HD2, respectively, were cloned by Rapid Amplification of cDNA Ends (RACE). a1 gene is 1428 bp in full-length, having 48% similarity to HD1 of Pleurotus djamor, and also with Nuclear Localization Signal (NLS):RKRRR, while a2 gene is 1680 bp in full length, having 39% similarity to conserved HD2 of P. djamor, and also with NLS:RRSRCRKE. By polymerase chain reaction using specific primers derived from mating genes a1, a2, b1, b2, PR3, PR5, PR7, PR8, PR15 and PR19, A1 mating type was demonstrated possessing a1, a2 genes; A2 mating type b1, b2 genes; B1 PR3, PR5, PR15 and PR19 genes; B2 PR3, PR7 and PR8 genes, respectively. The mating genes in A1B1, A1B2, A2B1, A2B2 of G. lucidum were further verified by dual mating in vitro on YMSA plates based on cytology, and ontology of clamp connection and pseudoclamp connection formation. And also, two binary vectors pCGl-a1 and pCGl-a2, having the a1 or a2 gene insertions, driven by glyceraldehyde - 3 - phosphate dehydrogenase (gpd) promoter and the downstream hygromycin resistance gene, were constructed, and will be transformed into compatible mating type to prove the function of these genes in mating. In addition, a Fosmid library constructed from monokaryotic strain BCRC 37180 (A2B2) of G. lucidum screened by labeled b1 and PR7 gene probes indicated 8 clones showing positive signals against A locus genes, and 5 clones to B locus genes. These specific Fosmid clones will be both terminal ends sequenced and assembled with the EST and genome databases to constructed a complete A, B, mating type locus genetic map. We also constructed a subtracted cDNA library of G. lucidum, genes which differentially expressed during fruiting body development will be cloned and their function proved by gene disruption. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T16:30:04Z (GMT). No. of bitstreams: 1 ntu-94-R92633011-1.pdf: 2833801 bytes, checksum: d74c81560d5ddbbbd937d9fcaa1c2dd5 (MD5) Previous issue date: 2005 | en |
dc.description.tableofcontents | 中文摘要...................................................................................................1
Abstract......................................................................................................3 前 言..........................................................................................................5 壹 、前人研究.........................................................................................6 一 、靈芝的分類學地位及相關研究...............................................6 二 、靈芝交配型之研究..................................................................7 三 、擔子菌模式種生物交配型之研究..........................................7 (一) 交配型因子A與B之經典研究.......................................7 (1) 交配型因子位置之研究.................................................7 (2) 交配型因子調節菌絲型態發展之研究.........................8 (3) 交配型因子突變種之研究.............................................9 (二) 現代分子遺傳學層次對於交配型因子A之研究..........10 (1) 位在A mating type locus上的基因..............................10 (2) Homeodomain蛋白質..................................................11 (3) 擔子菌中的homeodomain蛋白質...............................12 (三) 現代分子遺傳學層次對於交配型因子B之研究..........13 (1) 位在B mating type locus上的基因.............................13 (2) 酵母菌之pheromone與pheromone receptor...............15 (3) 高等擔子菌之pheromone與pheromone receptor.......16 (4) pheromone與pheromone receptor之辨認...................17 四 、擔子菌出菇相關基因之研究..................................................18 (一) Pseudoclamp connection formation 1 (pcc1)基因...........19 (二) Clampless 1 (clp1)基因....................................................19 (三) Ichijiku 1 (ich1)基因........................................................20 (四) Elongationless 2 (eln2)基因.............................................20 (五) Elongationless 3 (eln3)基因.............................................21 (六) rad 9 基因.......................................................................21 (七) Meiotic recombination 11 (mre11)基因...........................22 (八) spo11基因.......................................................................22 (九) B-regulated transcript 1 (brt1)基因..................................23 (十) mtd1基因.........................................................................23 (十一) FRT1基因.....................................................................23 (十二) SC4基因.......................................................................24 (十三) 在模式種生物外所發現與出菇相關的基因...............25 貳 、材料與方法...................................................................................26 一 、靈芝菌株之來源與保存........................................................26 二 、靈芝之培養............................................................................26 三 、靈芝之核酸萃取與分析........................................................28 (一) 靈芝Genomic DNA萃取................................................28 (二) DNA洋菜電泳膠分析.....................................................30 (三) 靈芝Total RNA萃取.......................................................32 (四) RNA Formaldehyde變性電泳膠分析.............................34 四 、聚合酵素鏈鎖反應.................................................................35 (一) PCR (polymerase chain reaction) ....................................35 (二) RT-PCR (Reverse Transcriptase-PCR).............................36 五 、PCR-Select cDNA Subtraction...............................................37 (一) 合成subtraction所需之cDNA........................................38 (二) PCR-select subtraction.....................................................40 六 、RNA-ligase-medicated Rapid amplification of cDNA ends (RLM-RACE) .........................................................................45 (一) Dephosphorylating RNA..................................................46 (二) Removing the mRNA Cap Structure................................46 (三) Ligating the RNA Oligo to Decapped mRNA.................47 (四) Reverse Transcribing mRNA........................................47 (五) Amplifying 5’ cDNA Ends..............................................48 (六) Amplifying 3’ cDNA Ends..............................................49 (七) Nested PCR for Amplifying 5’ cDNA Ends.....................50 (八) Nested PCR for Amplifying 3’ cDNA Ends.....................50 (九) 電泳膠純化DNA片段...................................................51 (十) T and A cloning................................................................52 (十一) Colony check................................................................53 (十二) 質體小量萃取..............................................................53 七 、Fosmid Library Screen...........................................................55 (一) DIG-labeled probe...........................................................55 (二) Fosmid colony hybridization...........................................55 八 、Plasmid construction..............................................................57 (一) 建構pGlGT帶有合成之GlGPD+Terminator片段......57 (二) 建構pGlGH帶有合成之GlGPD+ hpt +35S 3’UTR片段.....................................................................................58 (三) 電穿孔competent cell製備............................................59 (四) 電穿孔(electroporation)轉型...........................................60 (五) pCAM7000 (HygR gene deleted) plasmid建構..............60 (六) pCAM−Hyg plasmid 建構.............................................61 (七) pCGl plasmid 建構........................................................61 (八) a1與a2基因之cDNA全長度選殖.............................62 (九) pCGl-a1 與pCGl-a2 plasmid建構..............................63 九 、Agrobacterium – Medated - Transformation............................64 十 、Sequence analysis...................................................................65 (一) 一般序列之比對與分析..................................................65 (二) Subtraction之結果序列比對分析...................................66 (三) 胺基酸的序列分析..........................................................67 (四) 親緣演化分析..................................................................67 參 、結果................................................................................................68 一 、Mating相關基因之結果........................................................68 (一) 靈芝mating type a2基因................................................68 (二) 靈芝mating type a1基因................................................70 (三) 靈芝mating type b1與b2基因......................................71 (四) 靈芝的pheromone與pheromone receptor gene............71 (五) 不同交配型靈芝的mating gene分析............................72 二 、Subtraction與fruiting相關基因之結果...............................73 三 、Fosmid Library Screening......................................................74 四 、Plasmid Construction..............................................................75 (一) 靈芝glyceraldehyde - 3 - phosphate dehydrogenase ( gpd )基因..................................................................................75 (二) 建構pGlGT帶有合成之GlGPD + Terminator片段......76 (三) 建構pGlGH帶有合成之GlGPD + hpt + 35S 3’UTR片段....................................................................................77 (四) 電穿孔(electroporation)轉型..........................................78 (五) pCAM-Hyg plasmid 建構.............................................78 (六) pCGl plasmid 建構..........................................................79 (七) a1與a2基因之cDNA全長度選殖...............................79 肆 、討論................................................................................................80 一 、靈芝位於A locus上之交配型相關基因...............................80 二 、靈芝位於B locus上之交配型相關基因...............................83 三 、靈芝mating相關基因於不同交配型分布的情形................85 四 、靈芝出菇相關基因.................................................................86 五 、未來的展望.............................................................................88 伍 、圖....................................................................................................89 陸 、表.......................................................................................,,.........111 柒 、參考文獻......................................................................................131 捌 、附錄..............................................................................................144 玖 、附圖..............................................................................................151 | |
dc.language.iso | zh-TW | |
dc.title | 靈芝交配型與出菇相關基因之選殖與分析 | zh_TW |
dc.title | Molecular cloning and characterization of mating type and fruiting related genes in Ganoderma lucidum | en |
dc.type | Thesis | |
dc.date.schoolyear | 93-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蔣丙煌,蔡世峰,蕭明熙,劉瑞芬 | |
dc.subject.keyword | 靈芝,交配型,交配基因,費洛蒙,費洛蒙受體,出菇, | zh_TW |
dc.subject.keyword | Ganoderma lucidum,fruiting,homeodomain protein,mating type gene,pheromone pheromone receptor, | en |
dc.relation.page | 155 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2005-07-12 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
顯示於系所單位: | 植物病理與微生物學系 |
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