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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/38226完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 翁秀貞 | |
| dc.contributor.author | Chien-Hsin Chu | en |
| dc.contributor.author | 朱建勳 | zh_TW |
| dc.date.accessioned | 2021-06-13T16:28:19Z | - |
| dc.date.available | 2005-08-02 | |
| dc.date.copyright | 2005-08-02 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-14 | |
| dc.identifier.citation | 1. Alderete, J. F., D. P. and M. W. Lehker (1995) Iron mediates Trichomonas vaginalis resistance to complement lysis. Microbial Pathogenesis 19:93–103.
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Upcroft (2003) Drug resistance in the sexually transmitted protozoan Trichomonas vaginalis. Cell Res. 13:239-249. 13. Dyall, S. D., W. Yan, M. G. Delgadillo-Correa, A. Lunceford, J. A. Loo, C. F. Clarke and P. J. Johnson (2004) Non-mitochondrial complex I proteins in a hydrogenosomal oxidoreductase complex. Nature 431:1103-1107. 14. Engbring, J. A. and J. F. Alderete (1998) Three genes encode distinct AP33 proteins involved in Trichomonas vaginalis cytoadherence. Mol. Microbiol. 28:305-313. 15. Garcia, A. F., T. H. Chang, M. Benchimol, D. J. Klumpp, M. W. Lehker and J. F. Alderete (2003) Iron and contact with host cells induce expression of adhesions on surface of Trichomonas vaginalis. Mol. Microbiol. 47:1207-1224. 16. Hrdy, I., R. P. Hirt, P. Dolezal, L. Bardonova´, P. G. Foster, J. Tachezy and T. M. Embley (2004) Trichomonas hydrogenosomes contain the NADH dehydrogenase module of mitochondrial complex I. Nature 432:618-622. 17. Keister, D. (1983) Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Transaction and The Royal Society of Tropical Medicine and Hygiene 72:431-432 18. Laga, M., A. Manoka, M. Kivuvu, B. Malele, M. Tuliza, N. Nzila, J. B. Goeman, F. V. Batter, M. Alary (1993) Non-ulcerative sexually transmitted diseases as risk factors for HIV-1 transmission in women: results from a cohort study. AIDS 7:95–102. 19. Laga, M., M. Alary, N. Nzila, A. T. Manoka, M. Tuliza, F. Behets, J. S. Goeman, M. Louis, and P. Piot (1994) Condom promotion, sexually transmitted disease treatment, and declining incidence of HIV-1 infection in female Zairian sex workers. Lancet 344:246–248. 20. Lau, A., D. R. Liston , S. Vanacova and P. J. Johnson (2003) Trichomonas vaginalis initiator binding protein, IBP39, contains a novel DNA binding motif. Mol. Biochem. Parasitol. 130:167-171 21. Lehker, M. W., R. Arroyo and J. F. Alderete (1991) The regulation by iron of the synthesis of adhesions and cytoadherence levels in the protozoan Trichomonas vaginalis. J Exp Med. 174:311-318 22. Liston, D. R. and P. J. Johnson（1998）Gene Transcription in Trichomonas vaginalis. Parasitol Today. 14:261-265. 23. Liston, D. R. and P. J. Johnson (1999) Analysis of a ubiquitous promoter element in a primitive eukaryote: early evolution of the initiator element. Mol. Cell. Biol. 19:2380-2388. 24. Liston, D. R., A. O. T. Lau, D. Ortiz, S. T. Smale and P. J. Johnson (2001) Initiator Recognition in a Primitive Eukaryote: IBP39, an Initiator-Binding Protein from Trichomonas vaginalis. Mol. Cell. Biol. 21:7872-7882 25. Marmorstein, R. (2003) Transcription initiation at its most basic level. Cell 115:370-372 26. Moreno-Brito V., Yanez-Gomez C., Meza-Cervantez P., Avila-Gonzalez L., Rodriguez M.A., Ortega-Lopez J., Gonzalez-Robles A. and R. Arroyo (2005) A Trichomonas vaginalis 120 kDa protein with identity to hydrogenosome pyruvate:ferredoxin oxidoreductase is a surface adhesin induced by iron. Cell Microbiol. 7:245-258. 27. Mundodi, V., A. S. Kucknoor, D. J. Klumpp, T. H. Chang and J. F. Alderete (2004) Silencing the ap65 gene reduces adherence to vaginal epithelial cells by Trichomonas vaginalis. Mol.Microbiol. 53:1099-1108. 28. O’Brien, J. L., C. M. Lauriano and J. F. Alderete (1996) Molecular characterization of a third malic enzyme-like AP65 adhesin gene of Trichomonas vaginalis. Microbial pathogenesis 20:335-349. 29. Ong, S. J., S. C. Huang, H. W. Liu and J. H. Tai (2004) Involvement of multiple DNA element in iron-inducible transcription of the ap65-1 gene in the protozoan parasite Trichomonas vaginalis. Mol.Microbiol. 52:1721-1730. 30. Schumacher, M. A., A. O. T. Lau, and P. J. Johnson (2003) Structural basis of core promoter recognition in a primitive eukaryote. Cell 115:413-424. 31. Smale, S. T. and J. T. Kadonaga (2003) The RNA polymerase II core promoter. Annu. Rev. Biochem. 72:449-479. 32. Soper, D. (2004) Trichomoniasis: Under control or undercontrolled? Am. J. Obstet. Gynecol. 190:281-90 33. Sutak, R., P. Dolezal, H. L. Fiumera, I. Hrdy, A. Dancis, M. Delgadillo-Correa, P. J. Johnson, M. Muller, and J. Tachezy (2004) Mitochondrial-type assembly of FeS centers in the hydrogenosomes of the amitochondriate eukaryote Trichomonas vaginalis. PNAS 101:1038-10373. 34. Tai, J. H., Su, H.M. Tsai, J., Shaio, M. F. and Wang C.C. (1993) The divergence of Trichomonas vaginalis virus RNAs among various isolates of Trichomonas vaginalis. Expermental Parasitology. 76:278-286. 35. Tocher, J. H., and D. I. Edwards (1994) Evidence for the direct interaction of reduced metronidazole derivatives with DNA bases. Biochem. Pharmacol. 48:1089–1094. 36. Tsai, C. D., H. W Liu. and J. H. Tai (2002) Characterization of an Iron- responsive Promoter in the Protozoan Pathogen Trichomonas vaginalis. The Journal of Biological Chemistry. 227:5153-5162. 37. Vanacovaa, S., D. R. Listona, J. Tachezyb, P. J. Johnson (2003) Molecular biology of the amitochondriate parasites, Giardia intestinalis, Entamoeba histolytica and Trichomonas vaginalis. International Journal for Parasitology 33:235-255. 38. Wang, A., C. C. Wang, and J. F. Alderete (1987) Trichomonas vaginalis phenotypic variation occurs only among trichomonads infected with the double-stranded RNA virus. J. Exp. Med. 166:142-150. 39. 黃勝祈 (2002) 探討鐵離子級細胞生長對陰道滴蟲ap65-1啟動子的調控表現. 台灣大學微生物學研究所寄生蟲學組碩士論文. 40. 許弘明 (2004) 陰道滴蟲tvMyb 家族蛋白質生化特性分析. 台灣大學微生物學研究所寄生蟲學組碩士論文. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/38226 | - |
| dc.description.abstract | 在過去對陰道滴蟲ap65-1啟動子的研究中，了解ap65-1啟動子的活性會受到生長以及鐵離子的正向調控，而ap65-2啟動子也受到類似的正向調控。而在本研究中將培養液中的鐵離子進一步降低後，對於ap65-1啟動子調控有更深入的認識。
ap65-1啟動子內包含數個核蛋白結合序列，其中MRE-1/MRE-2r和MRE-2f區域對基礎活性與鐵調控都有相當的重要性，而且MRE-1，MRE-2r分別突變有不一致的變化，顯示此區域的調控並非單一啟動子元素所主控。基礎活性可能受MRE-1、MRE-2f、AGTGA區域負向調控，生長活性可能受MRE-1/MRE-2r及AGTGA區域正向調控，鐵活性可能受MRE-2f正向調控以及MRE-2r和AGTGA區域的負向調控。 由近化學平衡培養的實驗中發現，當培養環境較穩定時，ap65-1啟動子的生長調控即消失，故生長調控可能是因為環境變化所誘發出的反應。 另一方面在ap65-2的啟動子調控分析，由啟動子刪短實驗得知影響ap65-2啟動子活性的最高的區域為-112~-160之間，此區域中又以T-rich區域的突變對活性影響最大，故其週遭區域可能有其他重要的調控相關序列存在。 | zh_TW |
| dc.description.abstract | Previous research showed that ap65-1 promoter is positively regulated by iron and growth. And ap65-2 promoter has similar regulation character. In this research, we deplete the iron in the medium, it could help us more deeply to understand the regulation of ap65-1 promoter.
Several nuclear protein binding sequences exist in ap65-1 promoter. MRE-1/MRE-2r and MRE-2f regions are very important to basal activity and iron-inducible activity. And individually mutating MRE-1 and MRE-2r result in different activity change. This result show the MRE-1/MRE-2r region is not a single regulation unit. Basal activity might be negatively regulated by MRE-1, MRE-2f and AGTGA regions. Growth-related activity might be positively regulated by MRE-1/MRE-2r and AGTGA regions. Iron-inducible activity might be positively regulated by MRE-2f region, and negatively regulated by MRE-2r and AGTGA regions. According to experiment of the semi-chemostatic culture, when culture environment is stable, the growth-related activity of ap65-1 promoter disappear. Suggesting the growth-related activity might result form environment change. In the analysis of ap65-2 promoter, we discover the most important region to ap65-2 promoter activity is between -112~-160. In this range, the mutation of T-rich region could result to the hugest influence to ap65-2 promoter activity. Suggesting maybe some important regulation regions are located near T-rich region. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T16:28:19Z (GMT). No. of bitstreams: 1 ntu-94-R91445203-1.pdf: 456546 bytes, checksum: ed2dbee0965b4aa86173350db3749763 (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 目錄
縮寫表．．．．．．．．．．．．．．．．．．．１ 英文摘要．．．．．．．．．．．．．．．．．．２ 中文摘要．．．．．．．．．．．．．．．．．．３ 前言．．．．．．．．．．．．．．．．．．．．４ 一、陰道滴蟲簡介．．．．．．．．．．．．．．．．．４ 二、陰道滴蟲的特殊胞器─成氫體及相關代謝路徑．．．４ 三、鐵離子濃度對陰道滴蟲的影響．．．．．．．．．．４ 四、陰道滴蟲的基因轉錄．．．．．．．．．．．．．．５ 五、陰道滴蟲之黏著蛋白質．．．．．．．．．．．．．６ 六、ap65-1，ap65-2啟動子及相關基因調控．．．．．．７ 材料方法．．．．．．．．．．．．．．．．．．８ 一、陰道滴蟲細胞株的培養．．．．．．．．．．．．．８ 二、轉殖質體的建構與萃取．．．．．．．．．．．．．８ 三、陰道滴蟲轉染與選殖．．．．．．．．．．．．．．９ 四、陰道滴蟲蟲株之保存與活化．．．．．．．．．．１０ 五、啟動子活性分析．．．．．．．．．．．．．．．１１ 六、萃取陰道滴蟲DNA．．．．．．．．．．．．．．１１ 七、點墨點染色法．．．．．．．．．．．．．．．．１２ 八、近化學平衡培養法．．．．．．．．．．．．．．１２ 結果．．．．．．．．．．．．．．．．．．．１３ 一、穩定轉殖蟲株WT13活性表現特性．．．．．．．１３ 二、ap65-1啟動子的調控區域分析．．．．．．．．．１３ 三、近化學平衡培養法．．．．．．．．．．．．．．１５ 四、ap65-2啟動子的調控分析．．．．．．．．．．．１５ 討論．．．．．．．．．．．．．．．．．．．１７ 一、ap65-1啟動子的基礎活性．．．．．．．．．．．１７ 二、ap65-1啟動子MRE-1/MRE-2r重疊區域的分析．．１７ 三、ap65-1啟動子的調控與Myb蛋白質的關係．．．．１８ 四、影響ap65-1啟動子生長調控的因子．．．．．．．２０ 五、ap65-2啟動子的調控．．．．．．．．．．．．．２１ 附圖．．．．．．．．．．．．．．．．．．．２３ 附表．．．．．．．．．．．．．．．．．．．３９ 參考文獻．．．．．．．．．．．．．．．．．４４ | |
| dc.language.iso | zh-TW | |
| dc.subject | 生長 | zh_TW |
| dc.subject | 調控 | zh_TW |
| dc.subject | 鐵 | zh_TW |
| dc.subject | ap65啟動子 | zh_TW |
| dc.subject | 陰道滴蟲 | zh_TW |
| dc.subject | Trichomonas vaginalis | en |
| dc.subject | ap65promoter | en |
| dc.subject | iron | en |
| dc.subject | growth | en |
| dc.subject | regulation | en |
| dc.subject | MRE | en |
| dc.subject | Myb | en |
| dc.title | 陰道滴蟲ap65基因之啟動子調控分析 | zh_TW |
| dc.title | Promoter analysis of the ap65 genes in Trichomonas vaginalis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 戴榮湘,許翠瑛 | |
| dc.subject.keyword | 陰道滴蟲,ap65啟動子,鐵,生長,調控, | zh_TW |
| dc.subject.keyword | Trichomonas vaginalis,ap65promoter,iron,growth,regulation,MRE,Myb, | en |
| dc.relation.page | 47 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-07-14 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
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