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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳俊宏(Jiun-Hong Chen) | |
| dc.contributor.author | Tzu-Lun Tseng | en |
| dc.contributor.author | 曾子倫 | zh_TW |
| dc.date.accessioned | 2021-05-13T08:37:08Z | - |
| dc.date.available | 2016-08-24 | |
| dc.date.available | 2021-05-13T08:37:08Z | - |
| dc.date.copyright | 2016-08-24 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-08-02 | |
| dc.identifier.citation | Agata, K., Saito, Y., & Nakajima, E. (2007). Unifying principles of regeneration I: Epimorphosis versus morphallaxis. Dev Growth Differ, 49(2), 73-78.
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Zhang, Z., Song, T., Jin, Y., Pan, J., Zhang, L., Wang, L., & Li, P. (2009). Epidermal growth factor receptor regulates MT1-MMP and MMP-2 synthesis in SiHa cells via both PI3-K/AKT and MAPK/ERK pathways. Int J Gynecol Cancer, 19(6), 998-1003. Zoran, M. J. (2010). Regeneration in Annelids. doi:10.1002/9780470015902.a0022103 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/3820 | - |
| dc.description.abstract | 蛋白酶參與在生物體內許多重要的生理機制，像是消化、凝血及組織重組等。而在眾多蛋白酶中，金屬蛋白酶是一群具有最多元酵素功能的蛋白酶，其中基質金屬蛋白酶(Matrix metalloproteinase, MMP)、ADAM及ADAMST為金屬蛋白酶中主要的三個類群，他們都需要金屬離子的協助才能具有正常的酵素活性。而MMP是目前具有最多研究的金屬蛋白酶，他們是主要調控細胞外間質(Extracellular matrix)的分解及構成，已被發現參與在發育及再生過程中的細胞增生及移動。雖然在斑馬魚及渦蟲中MMP已被發現參與再生的過程，但MMP在再生中的機制仍不甚清楚。由於淡水生的環節動物瓢體蟲 (Aeolosoma viride) 具有很強的再生能力，並且使用和脊椎動物類似的機制來進行再生，因此在此篇研究中被用來進行MMP與再生的研究。在此論文的研究結果中，選殖出瓢體蟲中三種基質金屬蛋白酶，其中兩種是鑲嵌在膜上的MMP (MT-MMP)而另外一種是分泌型的MMP。其中兩種MMP在再生早期會高量表現於新生成的組織中，顯示該兩種MMP可能參與在再生的早期過程。進一步去檢測在再生過程中MMP的活性變化，發現到MMP在再生24小時的時候會在新再生組織中具有很高的酵素活性。再以MMP活性的抑制劑GM6001去抑制在再生中MMP的活性，來了解MMP在再生過程中的功能，發現到只有在再生0-12小時內抑制酵素活性會顯著地降低再生的成功率。依據這些結果都確實MMP參與了瓢體蟲的早期頭部再生。 | zh_TW |
| dc.description.abstract | Proteases play important roles in many biological processes. Among these proteases, metalloproteases represent the largest catalytic classes of proteases in many organisms. The three major classes of metalloproteinases are ADAMs (a disintegrin and metalloproteinase domain), ADAMTS (ADAMs with thrombospondin domain), and MMP (matrix metalloproteinase). MMPs play key roles in turnover of extracellular matrix (ECM) and serve as important regulators of cell-ECM interaction during development and regeneration. Although MMPs were found involved in the regeneration in planarian and zebrafish, there is a knowledge gap between planarian and vertebrates. Therefore, the annelid was chosen to fill the gap due to the similarity of regenerative mechanism to vertebrate. In this study, a fresh water annelid with high regenerative ability, Aeolosoma viride, was used to solve this question. In the NGS transcriptome data of A. viride, three MMPs, named as Avi-MMP14, Avi-MMP21 and Avi-MMP17, and one MMP-like gene, Avi-MMP-like gene, were found. Gene expression of Avi-mmp21 and Avi-mmp17 significantly increased at the early stage of anterior regeneration. And, gene expression of Avi-mmp-like gene significantly increased at the late stage of anterior regeneration. However, Avi-mmp14 showed no differences in expression during anterior regeneration. On the other hand, SDS-PAGE gelatin zymography showed the highest protease activity was detected at 24 hours post amputation (hpa) blastema. Furthermore, after treated with GM6001, a MMPs inhibitor, at 0-12 hpa, regeneration was inhibited. These results suggested that MMPs have some effects on early stage of anterior regeneration, and Avi-MMP17 and Avi-MMP21 might be the key factors in this processes. The relationship between MMPs and the regulation of MMPs needs further research to be resolved. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-13T08:37:08Z (GMT). No. of bitstreams: 1 ntu-105-R03b21001-1.pdf: 4762514 bytes, checksum: 05c259d4a47eeae04c1dfb0026cd1280 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 口試委員審定書 i
致謝 ii 中文摘要 iii Abstract iv Introduction 1 Protease 1 Roles of MMPs in development and regeneration 2 Regeneration 4 Aeolosoma viride in regeneration research 7 Aims 8 Material and method 9 Aeolosoma viride 9 RNA extraction 9 Reverse transcription 10 Gene cloning 11 Real-time quantitative PCR 11 In situ hybridization 12 Zymograph 14 Statistic 14 Results 15 Sequencing of Avi-mmp14, Avi-mmp17, Avi-mmp21 and a MMP-like gene, Avi-mmp-like gene 15 The quantification gene expression of mmps in A. viride during anterior regeneration 17 Gene expression of Avi-mmps at the blastema during anterior regeneration 18 The enzymatic activity of Avi-MMPs at the early stage of anterior regeneration 19 Inhibition of the activity of MMPs causing impaired regeneration 20 The model of MMPs involved in the anterior regeneration in A. viride 21 Discussion 23 Reference 28 Figures 38 Figure 1: Sequences of Avi-mmps. 44 Figure 2: Phylogenetic tree of Avi-MMPs. 48 Figure 3: A MMP-like gene, Avi-MMP-like gene found related to anterior regeneration in A. viride. 50 Figure 4: Gene expression of Avi-mmps during the anterior regeneration. 52 Figure 5: Avi-mmp14 expressing localization during the anterior regeneration. 54 Figure 6: Avi-mmp17 expressing localization during the anterior regeneration. 56 Figure 7: Avi-mmp21 expressing localization during the anterior regeneration. 58 Figure 8: Zymograph showed the active MMPs in A. viride during regeneration. 60 Figure 9: The inhibitory effect of MMP inhibitor, GM6001, on anterior regeneration in A. viride. 63 Figure 10: The model of MMPs involved in the anterior regeneration in A. viride. 65 Tables 66 Table 1: Primers used for cloning Avi-mmp 66 Table 2: Primers used for the RNA probes 67 Table 3: Primers used for qPCR 67 | |
| dc.language.iso | en | |
| dc.subject | GM6001 | zh_TW |
| dc.subject | 瓢體蟲 | zh_TW |
| dc.subject | 再生 | zh_TW |
| dc.subject | 基質金屬蛋白? | zh_TW |
| dc.subject | GM6001 | en |
| dc.subject | Aeolosoma viride | en |
| dc.subject | regeneration | en |
| dc.subject | matrix metalloproteinases | en |
| dc.title | 基質金屬蛋白酶在瓢體蟲Aeolosoma viride前端再生扮演的角色 | zh_TW |
| dc.title | The Roles of Matrix Metalloproteinases in Anterior Regeneration in Aeolosoma viride | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 朱家瑩(Chia-Ying Chu),郭典翰(Dian-Han Kuo) | |
| dc.subject.keyword | 瓢體蟲,再生,基質金屬蛋白?,GM6001, | zh_TW |
| dc.subject.keyword | Aeolosoma viride,regeneration,matrix metalloproteinases,GM6001, | en |
| dc.relation.page | 67 | |
| dc.identifier.doi | 10.6342/NTU201601565 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2016-08-02 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生命科學系 | zh_TW |
| 顯示於系所單位： | 生命科學系 | |
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