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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張?仁 | |
| dc.contributor.author | Ju-Yi Chien | en |
| dc.contributor.author | 簡如一 | zh_TW |
| dc.date.accessioned | 2021-06-13T16:27:58Z | - |
| dc.date.available | 2011-07-26 | |
| dc.date.copyright | 2011-07-26 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-07-19 | |
| dc.identifier.citation | 1. Tan, Q., et al., Activation mutants in yeast RNA polymerase II subunit RPB3 provide evidence for a structurally conserved surface required for activation in eukaryotes and bacteria. Genes Dev, 2000. 14(3): p. 339-48.
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Ishihama, Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts. Nucleic Acids Res, 2003. 31(16): p. 4696-701. 18. Kimura, M., H. Suzuki, and A. Ishihama, Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II. Mol Cell Biol, 2002. 22(5): p. 1577-88. 19. Runner, V.M., V. Podolny, and S. Buratowski, The Rpb4 Subunit of RNA Polymerase II Contributes to Cotranscriptional Recruitment of 3' Processing Factors. Molecular and Cellular Biology, 2008. 28(6): p. 1883-1891. 20. Cai, G., et al., Mediator Head module structure and functional interactions. Nature Structural & Molecular Biology, 2010. 17(3): p. 273-279. 21. Hirtreiter, A., D. Grohmann, and F. Werner, Molecular mechanisms of RNA polymerase--the F/E (RPB4/7) complex is required for high processivity in vitro. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/38207 | - |
| dc.description.abstract | Gene expression involves several distinct stages, which are tightly controlled and coordinated. Although the detailed mechanism of each stage is well established, we are still lacking the linkage of how different processes are connected. In Saccharomyces cerevisiae, Rpb4p, which was discovered as a RNA polymerase subunit, is reported to involve in mRNA processing, mRNA export, translation and mRNA degradation, suggesting a role of coordinator. In this study, we aimed to explore the functional characters of RPB4 in both transcription and mRNA degradation in mammalian system. We found that RPB4 located at P-bodies, which accommodate translationally repressed mRNPs and proteins involved in mRNA degradation. In addition, RPB4 interacted with DCP1a, DCP2, DDX6, EDC3 and CAF1a in RNA-independent manner. By tethering RPB4 to luciferase reporter mRNA, we further provided evidences that RPB4 is functionally related to mRNA decay machinery. Interestingly, we observed that RPB4 can translocate from cytoplasm to nucleus and associate with phosporylated RNA polymerase II in response to heat shock stress. Knockdown of RPB4 resulted in down-regulation of proteins involved in mRNA metabolism. Taken together, these findings imply that RPB4 might modulate specific class mRNAs’ turnover by coordinating their metabolic processes. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T16:27:58Z (GMT). No. of bitstreams: 1 ntu-100-R98b46002-1.pdf: 1610836 bytes, checksum: d8cd9ee28a6dc4b18796d4b518378cc7 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 摘要……………………………………………………………………………………...i
Abstract………………………………………………………………………...………ii Contents………………………………………………………………………..……….iv I. Introduction RPB4 in transcription....................................................................................................1 RPB4 in post-transcription............................................................................................4 Deadenylation-dependent degradation pathway………………………………………5 Processing bodies..........................................................................................................6 II. Materials and Methods……………………………………………………………..8 III. Results……………………………………………………………………………..14 IV. Discussion ………….…………………………………………………………...…20 V. Figures Figure 1. RPB4 physically interacted with P-body components.................................26 Figure 2. RPB4 down-regulated the luciferase reporter expression............................29 Figure 3. Truncates of RPB4 had less interaction with P-body proteins…..….…….31. Figure 4. Knockdown P-body proteins restored the decrease of luciferase activities.34 Figure 5. RPB4 associated with RNA polymerase II in response to heat shock.……37 VI. Refferences ……………………………………………………………………......39 | |
| dc.language.iso | en | |
| dc.title | 核醣核酸聚合酶第四次單元RPB4在轉錄及訊息核醣核酸降解過程之功能分析 | zh_TW |
| dc.title | Functional Characterization of RNA Polymerase II Subunit RPB4 in Transcription and mRNA Decay Machinery | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 呂勝春,徐駿森,顏雪琪 | |
| dc.subject.keyword | 核醣核酸聚合酶,轉錄,處理體,核醣核酸降解,訊息核醣核酸代謝, | zh_TW |
| dc.subject.keyword | RNA polymerase II,transcription,P-body,mRNA decay,mRNA meatabolism, | en |
| dc.relation.page | 42 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-07-19 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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