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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 曾雪峰 | |
| dc.contributor.author | Hung-Hsin Tsai | en |
| dc.contributor.author | 蔡宏鑫 | zh_TW |
| dc.date.accessioned | 2021-06-13T15:55:16Z | - |
| dc.date.available | 2008-07-10 | |
| dc.date.copyright | 2008-07-10 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-06-16 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37991 | - |
| dc.description.abstract | 金屬表面的自由電子在適當的電磁波激發下，自由電子將會與電磁波耦合產生集體震盪的行為，此即所謂的表面電漿共振（surface plasmon resonance），其發生於金屬與介質交界面。表面電漿共振會因奈米粒子在不同形狀、大小下使金屬具有不同的光學特性。根據文獻記載，許多的癌細胞將會在細胞表面累積大量的表皮細胞生長因子接受體（epidermal growth factor receptor：EGFR），實驗上可利用抗體先與金屬奈米粒子結合，由於抗會體針對EGFR結合，且正常細胞和癌細胞的EGFR數目差異很大，故可標定細胞，而標定後的細胞在光譜或是影像上將會有很大的差異。
本論文使用時域有限差分法（finite-difference time-domain method），探討奈米粒子在細胞中造成的光學現象。本論文分析在不同金粒子大小下，奈米粒子在正常細胞與癌細胞中的光散射現象與對比度的影響；並探討使用OIT法（optical immersion technique）對於對比度的影響；與不同殼層厚度的二氧化矽/金奈米殼層粒子在細胞中所造成光散射現象的差異。金屬奈米粒子具備光學性質穩定、不會有光漂白（photobleaching）的現象，尤其是金（gold）具有對人體無毒性的優點，因此在生物細胞標定上的應用非常具有潛力。 | zh_TW |
| dc.description.abstract | Surface plasmon resonance (SPR) is a phenomenon of free electrons on the surface of metal coupled to the electromagnetic excitations exhibiting coherent collective oscillations. The optical properties of SPR of metal are sensitive to particle size, shape and the surrounding medium. The SPR of metal nanoparticles have special optical properties that can be applied for biomedical applications; nanoparticles can be conjugated with anti-epidermal growth factor receptor (anti-EGFR) antibodies that bind specifically to the cells due to the overexpressed EGFR on the cytoplasmic membrane of the cancerous cells. In this research, we build a simple model to describe the nanoparticles’ distribution in cancerous and normal cells. The finite-difference time-domain (FDTD) method and the Drude-CP (Drude-critical point) model are employed to simulate the nanoparticles in the biological cells. The calculation of total scattering cross-section (TSCS) spectrum and intensity contrast of nanoparticles in cancerous and normal cells, respectively, are compared. The optical immersion technique (OIT) effect of contrast is discussed. The silica/gold nanoshells in cells with different thickness of shells are also compared. Metal nanoparticles are in general optically stable, and resistive to photobleaching. In particular, gold nanoparticles are toxic-free, which makes them very suitable for human body. Therefore, metal nanoparticles have potential for labeling of biological cells and other applications. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T15:55:16Z (GMT). No. of bitstreams: 1 ntu-97-R95941079-1.pdf: 1567110 bytes, checksum: fa921e6a7fa43be4c7f5ae699875aec4 (MD5) Previous issue date: 2008 | en |
| dc.description.tableofcontents | 口試委員會審定書 Ⅰ
誌謝 Ⅱ 中文摘要 Ⅲ 英文摘要 Ⅳ 目錄 Ⅴ 圖目錄 Ⅶ 表目錄 ⅩⅠ 第一章 序論 1 1.1 前言 1 1.2 文獻回顧 2 1.3 本文內容 4 第二章 光與物質之交互作用 5 2.1 金屬的介電常數 5 2.2 表面電漿子（Surface Plasmon Polariton） 7 2.3 侷域性表面電漿子（Localized Surface Plasmon） 11 第三章 奈米粒子與生物細胞 14 3.1 簡介光學染劑 14 3.2 奈米粒子標定癌細胞之方法 16 3.3 建立奈米粒子在細胞中的分佈模型 19 第四章 時域有限差分法 21 4.1 FDTD演算法 21 4.2 Courant穩定準則 27 4.3 總場/散射場（Total-Field/Scattered-Field, TF/SF） 28 4.4 完美吸收邊界（PML） 30 4.5 近場至遠場轉換（Near-to-Far-Field Transformation） 33 4.6 Drude-CP model 36 第五章 數值模擬結果與分析 41 5.1 金奈米粒子在細胞中的影響 42 5.2 不同金奈米粒子的大小對TSCS頻譜與對比度的影響 49 5.3 OIT方法對於TSCS頻譜與對比度的影響 56 5.4 Nanoshells在細胞中對光散射的影響 59 第六章 結論與未來展望 65 6.1 結論 65 6.2 未來展望 66 參考文獻 67 | |
| dc.language.iso | zh-TW | |
| dc.title | 金奈米粒子應用於細胞標定之模擬與分析 | zh_TW |
| dc.title | Simulation and Analysis of Cellular Labeling with Gold Nanoparticles | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 江衍偉,張世慧 | |
| dc.subject.keyword | 時域有限差分法,表面電漿共振,金,奈米粒子,奈米殼層粒子,細胞標定, | zh_TW |
| dc.subject.keyword | FDTD method,Drude-CP model,surface plasmon resonance,gold,nanoparticles,nanoshells,cellular labeling, | en |
| dc.relation.page | 71 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2008-06-17 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 光電工程學研究所 | zh_TW |
| 顯示於系所單位： | 光電工程學研究所 | |
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