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標題: | 解脂酵素固定在不同官能基及孔徑之短通道介孔材料SBA-15上的應用與鑑定 Immobilization of Lipase in Short-channel Mesoporous SBA-15 Functionalized with Different Groups and Pore Diameters |
作者: | Chih-Chuan Huang 黃智詮 |
指導教授: | 鄭淑芬(Soofin Cheng) |
關鍵字: | 解脂酵素,三乙酸甘油酯,官能基,孔徑, lipase,triacetin,functionalized,pore size, |
出版年 : | 2008 |
學位: | 碩士 |
摘要: | 在合成溶液中藉由加入少量鋯(IV),可以合成出短通道之SBA-15。此外,變化合成中之水熱溫度,合成出三種不同孔徑之SBA-15 (7 nm, 8 nm, 9.2 nm),並且在孔洞材料上修飾各種不同官能基,例如,苯基、丙基硫、羧基、丙基胺和辛基。上述之孔洞材料皆用來當作解脂酵素(來自猪的胰臟)的載體,使解脂酵素固定在上面。
實驗結果發現,越大孔徑之SBA-15,對解脂酵素有越快的吸附效果。改變固定解脂酵素之pH 值發現,pH 為5 時,較適合含苯基、丙基硫、和羧基之SBA-15吸附解脂酵素,而pH 為7.4 時,較適合含丙基胺之SBA-15 吸附解脂酵素。在催化三乙酸甘油酯方面,自由解脂酵素在pH 為7.4 下活性最好,並且可以藉由再加入疏水之甲苯增加約1 倍的活性。 固定解脂酵素後之SBA-15,有著最高活性之材料為含丙基胺和辛基之SBA-15,在pH 為7.4 下催化三乙酸甘油酯水解,保有自由解脂酵素活性的五分之四。可能的原因為,丙基胺較不容易讓解脂酵素的結構變形而導致失活。此外,辛基為疏水性之官能基,三乙酸甘油酯較容易接近解脂酵素的活性中心。 Short-channel SBA-15 with three different pore diameters (7.0, 8.2, and 9.2 nm) were synthesized by hydrothermal reaction at different temperatures with the aid of a small amount of Zr(IV) in the synthesis solution. The mesoporous silica with various functional groups including phenol, thio, carboxylic acid, amino, and octyl were also prepared. They were used as the supports for lipase (from porcine pancreas) immobilization. The large pore facilitated the adsorption of the enzyme. The optimal pH value for immobilization of lipase changed with the functional groups, and pH 5 is the best for pure siliceous SBA-15 and those functionalized with phenol, thiol, and carboxylic acid while pH 7.4 is the best for amino functionalized SBA-15. In catalytic hydrolysis of triacetin, highest catalytic activity was achieved at pH 7.4 and in the presence of a small amount of hydrophobic co-solvent. Among the functionalized SBA-15 materials, the one functionalized with amino or octyl groups gave the highest catalytic activity. It is attributed to the amino group could keep in lipase at pH 7.4 through the electrostatic interaction and the octyl group facilitates the approach of triacetin to the active sites of lipase. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37438 |
全文授權: | 有償授權 |
顯示於系所單位: | 化學系 |
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