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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李士傑(Shyh-Jye Lee) | |
dc.contributor.author | Ku-Chi Tsao | en |
dc.contributor.author | 曹古驥 | zh_TW |
dc.date.accessioned | 2021-06-13T15:27:49Z | - |
dc.date.available | 2009-07-21 | |
dc.date.copyright | 2008-07-21 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-16 | |
dc.identifier.citation | Anliker, B., Chun, J., 2004a. Cell surface receptors in lysophospholipid signaling. Semin Cell Dev Biol. 15, 457-65.
Anliker, B., Chun, J., 2004b. Lysophospholipid G protein-coupled receptors. J Biol Chem. 279, 20555-8. Bandoh, K., et al., 1999. Molecular cloning and characterization of a novel human G-protein-coupled receptor, EDG7, for lysophosphatidic acid. J Biol Chem. 274, 27776-85. Bussmann, J., et al., 2007. Early endocardial morphogenesis requires Scl/Tal1. PLoS Genet. 3, e140. Chan, L. C., et al., 2007. LPA3 receptor mediates chemotaxis of immature murine dendritic cells to unsaturated lysophosphatidic acid (LPA). J Leukoc Biol. Chen, J., et al., 2006. Specific receptor subtype mediation of LPA-induced dual effects in cardiac fibroblasts. FEBS Lett. 580, 4737-45. Contos, J. J., Chun, J., 1998. Complete cDNA sequence, genomic structure, and chromosomal localization of the LPA receptor gene, lpA1/vzg-1/Gpcr26. Genomics. 51, 364-78. Contos, J. J., Chun, J., 2001. The mouse lpA3/Edg7 lysophosphatidic acid receptor gene: genomic structure, chromosomal localization, and expression pattern. Gene. 267, 243-53. Contos, J. J., et al., 2000. Lysophosphatidic acid receptors. Mol Pharmacol. 58, 1188-96. Detrich, H. W., 3rd, et al., 1995. Intraembryonic hematopoietic cell migration during vertebrate development. Proc Natl Acad Sci U S A. 92, 10713-7. Dooley, K. A., et al., 2005. Zebrafish scl functions independently in hematopoietic and endothelial development. Dev Biol. 277, 522-36. Ferry, G., et al., 2003. Autotaxin is released from adipocytes, catalyzes lysophosphatidic acid synthesis, and activates preadipocyte proliferation. Up-regulated expression with adipocyte differentiation and obesity. J Biol Chem. 278, 18162-9. Fukushima, N., et al., 2001. Lysophospholipid receptors. Annu Rev Pharmacol Toxicol. 41, 507-34. Goetzl, E. J., An, S., 1998. Diversity of cellular receptors and functions for the lysophospholipid growth factors lysophosphatidic acid and sphingosine 1-phosphate. Faseb J. 12, 1589-98. Hama, K., et al., 2006. Lysophosphatidic receptor, LPA3, is positively and negatively regulated by progesterone and estrogen in the mouse uterus. Life Sci. 79, 1736-40. Im, D. S., et al., 2000. Molecular cloning and characterization of a lysophosphatidic acid receptor, Edg-7, expressed in prostate. Mol Pharmacol. 57, 753-9. Ishii, I., et al., 2000. Functional comparisons of the lysophosphatidic acid receptors, LPA1/VZG-1/EDG-2, LPA2/EDG-4, and LPA3/EDG-7 in neuronal cell lines using a retrovirus expression system. Mol Pharmacol. 58, 895-902. Ishii, I., et al., 2004. Lysophospholipid receptors: signaling and biology. Annu Rev Biochem. 73, 321-54. Juarez, M. A., et al., 2005. Distinct roles for SCL in erythroid specification and maturation in zebrafish. J Biol Chem. 280, 41636-44. Kobashi, H., et al., 2006. Lysophospholipid receptors are differentially expressed in rat terminal schwann cells, as revealed by a single cell RT-PCR and in situ hybridization. Acta Histochem Cytochem. 39, 55-60. Lee, H., et al., 2000. Lysophosphatidic acid and sphingosine 1-phosphate stimulate endothelial cell wound healing. Am J Physiol Cell Physiol. 278, C612-8. Lin, C. I., et al., 2007. Lysophosphatidic acid regulates inflammation-related genes in human endothelial cells through LPA1 and LPA3. Biochem Biophys Res Commun. 363, 1001-8. Moolenaar, W. H., 2000. Development of our current understanding of bioactive lysophospholipids. Ann N Y Acad Sci. 905, 1-10. Moolenaar, W. H., et al., 2004. The ins and outs of lysophosphatidic acid signaling. Bioessays. 26, 870-81. Murakami, M., et al., 2008. Identification of the orphan GPCR, P2Y10 receptor as the sphingosine-1-phosphate and lysophosphatidic acid receptor. Biochem Biophys Res Commun. 371, 707-12. Noguchi, K., et al., 2003. Identification of p2y9/GPR23 as a novel G protein-coupled receptor for lysophosphatidic acid, structurally distant from the Edg family. J Biol Chem. 278, 25600-6. Pages, C., et al., 2001. Lysophosphatidic acid synthesis and release. Prostaglandins Other Lipid Mediat. 64, 1-10. Panetti, T. S., et al., 2000. Sphingosine-1-phosphate and lysophosphatidic acid stimulate endothelial cell migration. Arterioscler Thromb Vasc Biol. 20, 1013-9. Pasternack, S. M., et al., 2008. G protein-coupled receptor P2Y5 and its ligand LPA are involved in maintenance of human hair growth. Nat Genet. 40, 329-34. Patterson, L. J., et al., 2005. Scl is required for dorsal aorta as well as blood formation in zebrafish embryos. Blood. 105, 3502-11. Saatian, B., et al., 2006. Transcriptional regulation of lysophosphatidic acid-induced interleukin-8 expression and secretion by p38 MAPK and JNK in human bronchial epithelial cells. Biochem J. 393, 657-68. Saba, J. D., 2004. Lysophospholipids in development: Miles apart and edging in. J Cell Biochem. 92, 967-92. Shah, B. H., Catt, K. J., 2005. Roles of LPA3 and COX-2 in implantation. Trends Endocrinol Metab. 16, 397-9. Songhet, P., et al., 2007. fgf1 is required for normal differentiation of erythrocytes in zebrafish primitive hematopoiesis. Dev Dyn. 236, 633-43. Sumanas, S., Lin, S., 2006. Ets1-related protein is a key regulator of vasculogenesis in zebrafish. PLoS Biol. 4, e10. Tabata, K., et al., 2007. The orphan GPCR GPR87 was deorphanized and shown to be a lysophosphatidic acid receptor. Biochem Biophys Res Commun. 363, 861-6. Tanaka, M., et al., 2006. Autotaxin stabilizes blood vessels and is required for embryonic vasculature by producing lysophosphatidic acid. J Biol Chem. 281, 25822-30. Thisse, C., et al., 1994. Goosecoid expression in neurectoderm and mesendoderm is disrupted in zebrafish cyclops gastrulas. Dev Biol. 164, 420-9. Tokumura, A., et al., 2002. Identification of human plasma lysophospholipase D, a lysophosphatidic acid-producing enzyme, as autotaxin, a multifunctional phosphodiesterase. J Biol Chem. 277, 39436-42. Umezu-Goto, M., et al., 2002. Autotaxin has lysophospholipase D activity leading to tumor cell growth and motility by lysophosphatidic acid production. J Cell Biol. 158, 227-33. van Meeteren, L. A., et al., 2006. Autotaxin, a secreted lysophospholipase D, is essential for blood vessel formation during development. Mol Cell Biol. 26, 5015-22. Watanabe, N., et al., 2007. Plasma lysophosphatidic acid level and serum autotaxin activity are increased in liver injury in rats in relation to its severity. Life Sci. 81, 1009-15. Watsky, M. A., et al., 2000. Phospholipid growth factors and corneal wound healing. Ann N Y Acad Sci. 905, 142-58. Xie, Y., et al., 2002. Lysophosphatidic acid as an autocrine and paracrine mediator. Biochim Biophys Acta. 1582, 270-81. Ye, X., et al., 2005. LPA3-mediated lysophosphatidic acid signalling in embryo implantation and spacing. Nature. 435, 104-8. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37428 | - |
dc.description.abstract | 水解磷脂酸 (Lysophosphatidic acid, LPA) 廣泛存在生物體中,為一具有多種功能的生物活性脂類,其透過LPA受器參與多種生物性機制,包含細胞生長、分化、存活、凋亡。先前研究顯示LPA受器之一lpa3為內皮細胞分化基因,表現在血管內皮細胞,並可能參與血管生成機制。在本研究中,我選殖出一段斑馬魚lpa3基因含全部編碼區域 (coding region),利用RT-PCR發現lpa3基因表現於各個不同時期之早期胚更廣泛存在不同成體組織中,以原位雜合技術確認了lpa3基因在早期胚均有表現,而在受精後24小時高量表現於頭部及脊索上下區域。利用LPA3反義基因阻斷劑 (anti-sense morpholino oligonucleotides, zlpa3 MO),我發現zlpa3-MO可造成心臟循環障礙,水腫、血球及神經生成等缺陷。此等缺陷可因異位表現zlpa3而回復,此證明了zlpa3-MO的專一性及LPA3在此等生理作用之必需性。 | zh_TW |
dc.description.abstract | Lysophosphatidic acid (LPA) is a commonly existing bioactive lipid with multiple functions. Its functions are known to be mediated via its cognate G-protein-coupled receptors. It involves in many biological activities, including cell growth, differentiation, survival, apoptotic, and so on. LPA receptor 3 (EDG7) is a member of endothelial cell differentiation gene (EDG) family, which expresses in blood endothelial cells and is expected to participate in angiogenesis. However, the lpa3-null mice fail to show notable defects in angiogenesis. To further explore the role of LPA3, I have cloned an lpa3 gene with its complete sequence from zebrafish. Expression analysis by RT-PCR showed that zebrafish lpa3 (zlpa3) ubiquitously expresses in early embryos and adult tissues. By whole-mount in situ hybridization, it revealed that zlpa3 expresses abundantly in the head and regions surrounding notochord. Using the zlpa3 anti-sense morpholino oligonucleotides (zlpa3-MO), I observed that knockdown of zlpa3 resulted in defects in heart circulation, blood cell formation and neurogenesis. The zlpa3-MO resultant defects are specific since they could be rescued by ectopic expression of zlpa3. In conclusion, this study suggests that LPA3 plays an important role in regulating circulation, hematopoiesis and neurogenesis. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T15:27:49Z (GMT). No. of bitstreams: 1 ntu-97-R95b41014-1.pdf: 5040107 bytes, checksum: 2b812bcc28fab93c5d66646985ba14b6 (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | CONTENTS I
LIST OF FIGURES III LIST OF TABLES IV 中文摘要 1 ABSTRACT 2 INTRODUCTION 3 MATERIALS AND METHODS 6 Zebrafish Maintenance and Embryo Collection 6 Total RNA Isolation 6 Preparation of Single Strain cDNA 7 Zebrafish lpa3 Amplification 7 RT-PCR Analysis 8 Rapid Amplification of zlpa3 cDNA Ends 8 Sequence Analysis of zlpa3 9 Whole-Mount in situ Hybridization 9 Morpholino Anti-sense Oligonucleotids and Microinjection Procedures 9 zLPA3 tMO Efficiency Check 10 Microangiography 10 ο-dianisidine Blood Cell Staining 11 Statistical Analysis 11 RESULTS 12 Zebrafish lpa3 Gene Analysis 12 Zebrafish lpa3 Expression Analysis by RT-PCR 12 Zebrafish lpa3 Whole Mount In Situ Hybridization 13 zLPA3 tMO Efficiency Check 13 The Morphological Defects Induced by zlpa3 tMO Knockdown 14 The Zebrafish zlpa3 tMO Knockdown Affects on DLAV 14 The Zebrafish zlpa3 tMO Knockdown Affects on Hematopoiesis 15 The Zebrafish zlpa3 tMO Knockdown Affects on EG Formation 15 DISCUSSION 17 REFERENCES 21 FIGURES 26 TABLES 40 | |
dc.language.iso | en | |
dc.title | 水解磷脂酸受器3 (LPA3) 在斑馬魚早期胚發育所扮演角色之探討 | zh_TW |
dc.title | Roles of Lysophosphatidic Acid Receptor 3 (LPA3) in Zebrafish (Danio Rerio) Early Embryonic Development | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李心予,沈湯龍 | |
dc.subject.keyword | 水解磷脂酸,內皮細胞分化基因,水腫,心臟循環,血球生成,神經生成,斑馬魚, | zh_TW |
dc.subject.keyword | Lysophosphatidic acid,endothelial cell differentiation gene,heart circulation,hematopoiesis,neurogenesis, | en |
dc.relation.page | 45 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2008-07-17 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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