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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林淑萍(Shwu-Bin Lin) | |
| dc.contributor.author | Ping-Chen Chung | en |
| dc.contributor.author | 鍾秉真 | zh_TW |
| dc.date.accessioned | 2021-06-13T15:25:20Z | - |
| dc.date.available | 2010-08-13 | |
| dc.date.copyright | 2008-08-13 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-07-21 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37354 | - |
| dc.description.abstract | 二苯乙烯類 (stilbenoids)為植物抵抗黴菌或病蟲害的天然抗生素，許多研究報告指出，二苯乙烯類具有抗氧化、抗發炎、防癌及抗癌等生理活性。本論文研究純化自花生仁的二苯乙烯類，包括resveratrol (RSV)、arachindin-1 (Ara-1)、arachindin-3 (Ara-3)及isopentadienylresveratrol (IPD)的抗癌活性，以HL-60血癌細胞株當作實驗模型，研究中發現，RSV、Ara-1及Ara-3可抑制HL-60細胞株的生長，其中，Ara-1在較低的濃度就可以抑制細胞生長 (EC50為4.2 μM)。以Ara-1處理HL-60細胞，在短時間內可造成細胞粒線體膜電位的下降、caspases的活化、DNA斷裂以及細胞週期改變。Ara-3處理HL-60細胞則是觀察到其細胞膜完整性的改變、細胞粒線體膜電位的下降，以及caspases的活化。綜合以上結果，Ara-1及Ara-3處理HL-60細胞皆可以引發細胞走向凋亡，而對正常不增生的周邊血單核球細胞則無影響，因此具有成為抗癌藥物的潛力。由於體外試驗Ara-1可抑制Topoisomerase II的活性，但對HL-60與HL-60/MX2 (Topoisomerase II缺乏的HL-60突變株)的毒性沒有差異，推測Ara-1造成細胞走向凋亡並非透過抑制Topoisomerase II。但仍可由以上結果顯示，Ara-1及Ara-3可能具有抗癌的潛力。 | zh_TW |
| dc.description.abstract | Stilbenoids are phytoalexins produced by plants under attack by pathogens such as fungi. Previous sutdies have demonstrated that stilbenoids have bioactivities such as anti-oxidation, anti-inflammation, cancer chemoprevention and anti-carcinogenic effect. In this study, resveratrol (RSV), arachidin-1 (Ara-1), arachidin-3 (Ara-3), and isopentadienylresveratrol (IPD) were isolated from peanuts. RSV, Ara-1 and Ara-3 display cytotoxic effect on HL-60, and Ara-1 is the most toxic. Further study showed that Ara-1 treatment on HL-60 cells caused loss of mitochondrial membrane potential (MMP), caspases activation, and changes in cell cycle distribution. Ara-3 treatment on HL-60 cells caused loss of MMP and cell membrane integrity as well as caspases activation. These results suggested that Ara-1 and Ara-3 are potential anticancer agents that induced cancer cell apoptosis. Ara-1 and RSV are cytotoxic to proliferating cells, HL-60, but did not affect on nonproliferating human peripheral blood mononuclear cells. Furthermore, Ara-1 was found to inhibit the activity of topoisomerase II in vitro, but showed no significant difference in cytotoxicity between HL-60 and Topo II defective HL-60/MX2 cells. Topoisomerase II inhibition may not be involved in the mechanisms of apoptosis induction by Ara-1 in HL-60 cells. These results indicate that Ara-1 and Ara-3 are potential anti-cancer agents. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T15:25:20Z (GMT). No. of bitstreams: 1 ntu-97-R95424016-1.pdf: 1397996 bytes, checksum: eb17e7a4bc1c782a5a361875402ec659 (MD5) Previous issue date: 2008 | en |
| dc.description.tableofcontents | 中文摘要………………………………………………………………………………4
英文摘要………………………………………………………………………….......5 縮寫表…………………………………………………………………………………6 一、序論……………………………………………………………………………….7 1.1花生中的二苯乙烯類……………………………………………………….........7 1.2二苯乙烯類相關研究…………………………………………………………….7 1.3 癌症的治療…………………………………………………….........................8 1.4 細胞凋亡 (Apoptosis) ………………………................................................9 1.5 細胞週期 (Cell cycle) …………………………………………………..........11 1.6 拓樸異構酶II (Topoisomerase II) …………………………………………....12 1.7 研究目的……………………………………………......................................13 二、材料與方法………………………………………………………………..........14 2.1花生中二苯乙烯類的純化及穩定性探討……………………………………...14 2.2細胞培養………………………………………………………………………...14 2.3人類周邊血單核球細胞 (PBMC)的分離…………………………………......15 2.4細胞活性測定…………………………………………………………………...15 2.5細胞粒線體膜電位的測定……………………………………………………...16 2.6細胞內活性氧自由基 (reactive oxygen species; ROS)測定……………....16 2.7細胞DNA含量分析………………………………………………………….....16 2.8細胞膜完整性分析……………………………………………………………...16 2.9細胞內Caspases活性的測定………………………………………………....17 2.10 Topo II活性的測定…………………………………………………………....17 三、結果………………………………………………………………………….......18 3.1 花生中二苯乙烯類的純化……………………………………………………..18 3.2 Resveratrol、arachidin-1及arachidin-3能夠抑制HL-60細胞生長…….......18 3.3 Arachidin-1及resveratrol抑制人類血癌細胞株HL-60的生長而對不增生的PBMC則無影響……………………………………………..................................18 3.4 Arachidin-1處理引發HL-60細胞凋亡………………………………………..19 3.5 Arachidin-3處理引發HL-60細胞凋亡………………………………………..21 3.6 Arachidin-1及resveratrol抑制Topo II 活性………………………………...22 3.7 Arachidin-1不會與Topo II直接結合而抑制其活性………………………….22 3.8 Arachidin-1及arachidin-3對人類血癌細胞株HL-60與HL-60/MX2的毒性沒有差異……………………………………………………………………............23 3.9 Arachidin-1、arachidin-3及Isopentadienylresveratrol穩定性探討……...23 四、討論……………………………………………………………………….........24 五、參考文獻………………………………………………………………………..52 藥品清單………………………………………………………………………........59 | |
| dc.language.iso | zh-TW | |
| dc.subject | 細胞凋亡 | zh_TW |
| dc.subject | 二苯乙烯類 | zh_TW |
| dc.subject | 拓樸異構酶 | zh_TW |
| dc.subject | II | zh_TW |
| dc.subject | Topoisomerase II | en |
| dc.subject | Apoptosis | en |
| dc.subject | stilbenoids | en |
| dc.title | 落花生二苯乙烯類對HL-60細胞株的影響 | zh_TW |
| dc.title | The effects of peanut stilbenoids on HL-60 cell line | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 歐樂君(Lo-Chun Au),胡忠怡,邱義源 | |
| dc.subject.keyword | 二苯乙烯類,細胞凋亡,拓樸異構酶,II, | zh_TW |
| dc.subject.keyword | stilbenoids,Apoptosis,Topoisomerase II, | en |
| dc.relation.page | 59 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2008-07-21 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 醫學檢驗暨生物技術學研究所 | zh_TW |
| 顯示於系所單位： | 醫學檢驗暨生物技術學系 | |
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