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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄭景暉 | |
dc.contributor.author | Hung-Wei Yeh | en |
dc.contributor.author | 葉宏偉 | zh_TW |
dc.date.accessioned | 2021-06-13T15:24:09Z | - |
dc.date.available | 2008-08-13 | |
dc.date.copyright | 2008-08-13 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-22 | |
dc.identifier.citation | Aardema, M. J., S. Albertini, et al. (1998). 'Aneuploidy: a report of an ECETOC task force.' Mutat Res 410(1): 3-79.
Acosta, D., T. E. Stege, et al. (1986). 'Cytotoxicity of ethanol in primary cultures of rat midbrain neurons.' Toxicol Lett 30(3): 231-5. Adams, L. K., D. Y. Lyon, et al. (2006). 'Comparative toxicity of nano-scale TiO2, SiO2 and ZnO water suspensions.' Water Sci Technol 54(11-12): 327-34. Albertini, S., B. Miller, et al. (1997). 'Detailed data on in vitro MNT and in vitro CA: industrial experience.' Mutat Res 392(1-2): 187-208. Al-Hiyasat, A. S., H. Darmani, et al. (2005). 'Cytotoxicity evaluation of dental resin composites and their flowable derivatives.' Clin Oral Investig 9(1): 21-5. Atsumi, T., M. Ishihara, et al. (2004). 'Comparative radical production and cytotoxicity induced by camphorquinone and 9-fluorenone against human pulp fibroblasts.' J Oral Rehabil 31(12): 1155-64. Atsumi, T., I. Iwakura, et al. (2001). 'The production of reactive oxygen species by irradiated camphorquinone-related photosensitizers and their effect on cytotoxicity.' Arch Oral Biol 46(5): 391-401. Bakeeva, L. E., V. A. Zamyatnina, et al. (2001). 'Effect of the antioxidant ionol (BHT) on growth and development of etiolated wheat seedlings: control of apoptosis, cell division, organelle ultrastructure, and plastid differentiation.' Biochemistry (Mosc) 66(8): 850-9. Becher, R., R. B. Hetland, et al. (2001). 'Rat lung inflammatory responses after in vivo and in vitro exposure to various stone particles.' Inhal Toxicol 13(9): 789-805. Bowen, R. L. (1965). 'Adhesive bonding of various materials to hard tooth tissues. II. Bonding to dentin promoted by a surface-active comonomer.' J Dent Res 44(5): 895-902. Buonocore, M. G. (1955). 'A simple method of increasing the adhesion of acrylic filling materials to enamel surfaces.' J Dent Res 34(6): 849-53. Burke, F. J. and A. D. McCaughey (1995). 'The four generations of dentin bonding.' Am J Dent 8(2): 88-92. Carter, J. M. and K. E. Driscoll (2001). 'The role of inflammation, oxidative stress, and proliferation in silica-induced lung disease: a species comparison.' J Environ Pathol Toxicol Oncol 20 Suppl 1: 33-43. Catelas, I., A. Petit, et al. (2005). 'Quantitative analysis of macrophage apoptosis vs. necrosis induced by cobalt and chromium ions in vitro.' Biomaterials 26(15): 2441-53. Cerottini, J.C. and K. T. Brunner (1972). 'Reversible inhibition of lymphocyte-mediated cytotoxicity by cytochalasin B.' Nat New Biol 237(78): 272-3. Chang, H. H., M. K. Guo, et al. (2005). 'Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA.' Biomaterials 26(7): 745-53. Chen, R. S., C. C. Liu, et al. (2003). 'Cytotoxicity of three dentin bonding agents on human dental pulp cells.' J Dent 31(3): 223-9. Chen, R. S., C. C. Liuiw, et al. (2001). 'The effect of curing light intensity on the cytotoxicity of a dentin-bonding agent.' Oper Dent 26(5): 505-10. Chen, S. Y. and K. K. Sulik (1996). 'Free radicals and ethanol-induced cytotoxicity in neural crest cells.' Alcohol Clin Exp Res 20(6): 1071-6. Ciaravino, V., M. J. Suto, et al. (1993). 'High capacity in vitro micronucleus assay for assessment of chromosome damage: results with quinolone/naphthyridone antibacterials.' Mutat Res 298(4): 227-36. Dalal, N. S., X. L. Shi, et al. (1990). 'Role of free radicals in the mechanisms of hemolysis and lipid peroxidation by silica: comparative ESR and cytotoxicity studies.' J Toxicol Environ Health 29(3): 307-16. de Souza Costa, C. A., A. B. do Nascimento, et al. (2002). 'Response of human pulps following acid conditioning and application of a bonding agent in deep cavities.' Dent Mater 18(7): 543-51. de Souza Costa, C. A., A. B. Lopes do Nascimento, et al. (2001). 'Response of human pulps capped with a self-etching adhesive system.' Dent Mater 17(3): 230-40. Demarco, F. F., S. B. Tarquinio, et al. (2001). 'Pulp response and cytotoxicity evaluation of 2 dentin bonding agents.' Quintessence Int 32(3): 211-20. Eichmiller, F. C. (1993). 'A clinical perspective on dentin adhesives.' J Indiana Dent Assoc 72(5): 22-4. Engelmann, J., J. Volk, et al. (2005). 'ROS formation and glutathione levels in human oral fibroblasts exposed to TEGDMA and camphorquinone.' J Biomed Mater Res B Appl Biomater 75(2): 272-6. Erexson, G. L., M. V. Periago, et al. (2001). 'Differential sensitivity of Chinese hamster V79 and Chinese hamster ovary (CHO) cells in the in vitro micronucleus screening assay.' Mutat Res 495(1-2): 75-80. Fediukovich, L. V., V. Kotlovskii Iu, et al. (1988). '[Mutagenic and cytotoxic effects of acrylates].' Genetika 24(6): 1132-4. Fenech, M. (2000). 'The in vitro micronucleus technique.' Mutat Res 455(1-2): 81-95. Fenech, M. (2005). 'In vitro micronucleus technique to predict chemosensitivity.' Methods Mol Med 111: 3-32. Fenech, M. (2006). 'Cytokinesis-block micronucleus assay evolves into a 'cytome' assay of chromosomal instability, mitotic dysfunction and cell death.' Mutat Res 600(1-2): 58-66. Franz, A., F. Konig, et al. (2003). 'Cytotoxic effects of packable and nonpackable dental composites.' Dent Mater 19(5): 382-92. Fredericks, H. E. (1981). 'Mutagenic potential of orthodontic bonding materials.' Am J Orthod 80(3): 316-24. Fubini, B. and A. Hubbard (2003). 'Reactive oxygen species (ROS) and reactive nitrogen species (RNS) generation by silica in inflammation and fibrosis.' Free Radic Biol Med 34(12): 1507-16. Fusayama, T., M. Nakamura, et al. (1979). 'Non-pressure adhesion of a new adhesive restorative resin.' J Dent Res 58(4): 1364-70. Geurtsen, W. (2000). 'Biocompatibility of resin-modified filling materials.' Crit Rev Oral Biol Med 11(3): 333-55. Geurtsen, W., F. Lehmann, et al. (1998). 'Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblast cultures.' J Biomed Mater Res 41(3): 474-80. Gioka, C., C. Bourauel, et al. (2005). 'Light-cured or chemically cured orthodontic adhesive resins? A selection based on the degree of cure, monomer leaching, and cytotoxicity.' Am J Orthod Dentofacial Orthop 127(4): 413-9; quiz 516. Goldberg, M. (2008). 'In vitro and in vivo studies on the toxicity of dental resin components: a review.' Clin Oral Investig 12(1): 1-8. Grobler, S. R., A. Olivier, et al. (2004). 'Cytotoxicity of two concentrations of a dentine bonding agent on mouse 3T3 and human pulp fibroblast cell-lines.' SADJ 59(9): 368-70, 372. Heusele, C. and D. Bonne (1985). 'Role of DAPI in microtubule reactions at steady-state.' Biochem Biophys Res Commun 133(2): 662-9. Huget, E. F., J. C. Denniston, et al. (1979). 'Dental adhesives: a perspective.' Mil Med 144(9): 619-20. Ide, M., A. D. Morel, et al. (1998). 'The role of a dentine-bonding agent in reducing cervical dentine sensitivity.' J Clin Periodontol 25(4): 286-90. Jagetia, G. C. and S. K. Adiga (1998). 'Influence of vindesine exposure on the micronucleus formation and cell survival in V79 cells.' Mutat Res 421(1): 55-63. Jagielski, M., M. Zaleska, et al. (1976). '[Applicability of DAPI for the detection of Mycoplasms in cell cultures].' Med Dosw Mikrobiol 28(2): 161-73. Jin, Y., S. Kannan, et al. (2007). 'Toxicity of luminescent silica nanoparticles to living cells.' Chem Res Toxicol 20(8): 1126-33. Kanca, J., 3rd (1991). 'A method for bonding to tooth structure using phosphoric acid as a dentin-enamel conditioner.' Quintessence Int 22(4): 285-90. Kanca, J., 3rd (1991). 'A method for bonding to tooth structure using phosphoric acid as a dentin-enamel conditioner.' Quintessence Int 22(4): 285-90. Keate, K. C. and G. D. Woolsey (1988). 'The dentin-resin bond: a report on the 'classical' and 'new generation' adhesives.' Tex Dent J 105(12): 6-11, 57. Khatri, C. A., J. W. Stansbury, et al. (2003). 'Synthesis, characterization and evaluation of urethane derivatives of Bis-GMA.' Dent Mater 19(7): 584-8. Kipen, H. M. and D. L. Laskin (2005). 'Smaller is not always better: nanotechnology yields nanotoxicology.' Am J Physiol Lung Cell Mol Physiol 289(5): L696-7. Kirsch-Volders, M., M. Aardema, et al. (2000). 'Concepts of threshold in mutagenesis and carcinogenesis.' Mutat Res 464(1): 3-11. Kirsch-Volders, M., T. Sofuni, et al. (2003). 'Report from the in vitro micronucleus assay working group.' Mutat Res 540(2): 153-63. Kirsch-Volders, M., T. Sofuni, et al. (2000). 'Report from the In Vitro Micronucleus Assay Working Group.' Environ Mol Mutagen 35(3): 167-72. Koliniotou-Koubia, E., P. Dionysopoulos, et al. (2001). 'In vitro cytotoxicity of six dentin bonding agents.' J Oral Rehabil 28(10): 971-5. Kostrzewa, R. M. (2000). 'Review of apoptosis vs. necrosis of substantia nigra pars compacta in Parkinson's disease.' Neurotox Res 2(2-3): 239-50. Kugel, G. and M. Ferrari (2000). 'The science of bonding: from first to sixth generation.' J Am Dent Assoc 131 Suppl: 20S-25S. Lenz, A.G.,F. Krombach, et al. (1992). 'Oxidative stress in vivo and in vitro: modulation by quartz dust and hyperbaric atmosphere.' Free Radic Biol Med 12(1): 1-10. Lieberthal, W., V. Triaca, et al. (1996). 'Mechanisms of death induced by cisplatin in proximal tubular epithelial cells: apoptosis vs. necrosis.' Am J Physiol 270(4 Pt 2): F700-8. Lin, W., Y. W. Huang, et al. (2006). 'In vitro toxicity of silica nanoparticles in human lung cancer cells.' Toxicol Appl Pharmacol 217(3): 252-9. Marchetti, F. and A. J. Wyrobek (2003). 'PAINT/DAPI analysis of mouse zygotes to detect paternally transmitted chromosomal aberrations.' Adv Exp Med Biol 518: 131-45. Matsushima, T., M. Hayashi, et al. (1999). 'Validation study of the in vitro micronucleus test in a Chinese hamster lung cell line (CHL/IU).' Mutagenesis 14(6): 569-80. Medina, V. O., 3rd, K. Shinkai, et al. (2002). 'Histopathologic study on pulp response to single-bottle and self-etching adhesive systems.' Oper Dent 27(4): 330-42. Meng, H.,Z. Chen,et al.(2007). 'Ultrahigh reactivity provokes nanotoxicity: explanation of oral toxicity of nano-copper particles.' Toxicol Lett 175(1-3): 102-10. Meryon, S. D. and A. M. Brook (1989). 'In vitro cytotoxicity of three dentine bonding agents.' J Dent 17(6): 279-83. Miller, B., S. Albertini, et al. (1997). 'Comparative evaluation of the in vitro micronucleus test and the in vitro chromosome aberration test: industrial experience.' Mutat Res 392(1-2): 45-59, 187-208. Miller, B., F. Potter-Locher, et al. (1998). 'Evaluation of the in vitro micronucleus test as an alternative to the in vitro chromosomal aberration assay: position of the GUM Working Group on the in vitro micronucleus test. Gesellschaft fur Umwelt-Mutations-forschung.' Mutat Res 410(1): 81-116. Miyazaki, M., K. Iwasaki, et al. (2002). 'Adhesion of single application bonding systems to bovine enamel and dentin.' Oper Dent 27(1): 88-94. Mohsen, N. M., R. G. Craig, et al. (1998). 'Cytotoxicity of urethane dimethacrylate composites before and after aging and leaching.' J Biomed Mater Res 39(2): 252-60. Moodley, D., S. R. Grobler, et al. (2005). 'Cytotoxicity of a dentine bonding agent on four different cell-lines.' SADJ 60(6): 234-6. Morikawa, K. and M. Yanagida (1981). 'Visualization of individual DNA molecules in solution by light microscopy: DAPI staining method.' J Biochem 89(2): 693-6. Nassiri, M. R., C. T. Hanks, et al. (1994). 'Application of flow cytometry to determine the cytotoxicity of urethane dimethacrylate in human cells.' J Biomed Mater Res 28(2): 153-8. Nel, A., T. Xia, et al. (2006). 'Toxic potential of materials at the nanolevel.' Science 311(5761): 622-7. Newman, G. V., W. H. Snyder, et al. (1965). 'Adhesives and orthodontic attachments. (Preliminary investigation).' J N J State Dent Soc 37(3): 113-20 passim. Oberdorster, G., E. Oberdorster, et al. (2005). 'Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles.' Environ Health Perspect 113(7): 823-39. O'Driscoll, M. C., D. Scott, et al. (1998). 'Radiation-induced micronuclei in human fibroblasts in relation to clonogenic radiosensitivity.' Br J Cancer 78(12): 1559-63. Pagoria, D. and W. Geurtsen (2005). 'The effect of N-acetyl-l-cysteine and ascorbic acid on visible-light-irradiated camphorquinone/N,N-dimethyl-p-toluidine-induced oxidative stress in two immortalized cell lines.' Biomaterials 26(31): 6136-42. Palenik, C. J. and J. C. Setcos (1996). 'Antimicrobial abilities of various dentine bonding agents and restorative materials.' J Dent 24(4): 289-95. Pameijer, C. H. and H. R. Stanley (1995). 'Pulp reaction to a dentin bonding agent.' Am J Dent 8(3): 140-4. Paris, S., H. Meyer-Lueckel, et al. (2007). 'Penetration coefficients of commercially available and experimental composites intended to infiltrate enamel carious lesions.' Dent Mater 23(6): 742-8. Pashley, D. H., J. A. Horner, et al. (1992). 'Interactions of conditioners on the dentin surface.' Oper Dent Suppl 5: 137-50. Pashley, D. H. and F. R. Tay (2001). 'Aggressiveness of contemporary self-etching adhesives. Part II: etching effects on unground enamel.' Dent Mater 17(5): 430-44. Porter, D. W., L. L. Millecchia, et al. (2006). 'Nitric oxide and reactive oxygen species production causes progressive damage in rats after cessation of silica inhalation.' Toxicol Sci 90(1): 188-97. Prantera, G., M. Di Castro, et al. (1981). 'Inhibition of human chromosome condensation induced by DAPI as related to cell cycle.' Exp Cell Res 135(1): 63-8. Prati, C. and D. H. Pashley (1992). 'Dentin wetness, permeability and thickness and bond strength of adhesive systems.' Am J Dent 5(1): 33-8. Prica, D., N. Galic, et al. (2006). 'Genotoxicity evaluation of five different dentin bonding agents by chromosomal aberration analysis.' J Oral Rehabil 33(6): 462-71. Reichl, F. X., M. Seiss, et al. (2008). 'Toxicity potentiation by H2O2 with components of dental restorative materials on human oral cells.' Arch Toxicol 82(1): 21-8. Rocchi, A., M. Di Castro, et al. (1980). 'Effect of DAPI on Chinese hamster chromosomes.' Cytogenet Cell Genet 27(1): 70-2. Sayes, C. M., K. L. Reed, et al. (2007). 'Assessing toxicity of fine and nanoparticles: comparing in vitro measurements to in vivo pulmonary toxicity profiles.' Toxicol Sci 97(1): 163-80. Schedle, A., A. Franz, et al. (1998). 'Cytotoxic effects of dental composites, adhesive substances, compomers and cements.' Dent Mater 14(6): 429-40. Schweikl, H., A. Hartmann, et al. (2007). 'Inhibition of TEGDMA and HEMA-induced genotoxicity and cell cycle arrest by N-acetylcysteine.' Dent Mater 23(6): 688-95. Schweikl, H., K. A. Hiller, et al. (2005). 'Cytotoxic and mutagenic effects of dental composite materials.' Biomaterials 26(14): 1713-9. Schweikl, H. and G. Schmalz (1999). 'Triethylene glycol dimethacrylate induces large deletions in the hprt gene of V79 cells.' Mutat Res 438(1): 71-8. Schweikl, H., G. Spagnuolo, et al. (2006). 'Genetic and cellular toxicology of dental resin monomers.' J Dent Res 85(10): 870-7. Seagrave, J., J. L. Mauderly, et al. (2003). 'In vitro relative toxicity screening of combined particulate and semivolatile organic fractions of gasoline and diesel engine emissions.' J Toxicol Environ Health A 66(12): 1113-32. Seagrave, J., J. D. McDonald, et al. (2002). 'Mutagenicity and in vivo toxicity of combined particulate and semivolatile organic fractions of gasoline and diesel engine emissions.' Toxicol Sci 70(2): 212-26. Seagrave, J., J. D. McDonald, et al. (2005). 'In vitro versus in vivo exposure to combustion emissions.' Exp Toxicol Pathol 57 Suppl 1: 233-8. Seymour, C. B., C. Mothersill, et al. (1987). 'The effect of ethanol on the radiation response of CHO-K1 cells.' Br J Radiol 60(714): 577-81. Shackelford, R. E., W. K. Kaufmann, et al. (1999). 'Cell cycle control, checkpoint mechanisms, and genotoxic stress.' Environ Health Perspect 107 Suppl 1: 5-24. Shackelford, R. E., W. K. Kaufmann, et al. (2000). 'Oxidative stress and cell cycle checkpoint function.' Free Radic Biol Med 28(9): 1387-404. Sideridou, I. D. and D. S. Achilias (2005). 'Elution study of unreacted Bis-GMA, TEGDMA, UDMA, and Bis-EMA from light-cured dental resins and resin composites using HPLC.' J Biomed Mater Res B Appl Biomater 74(1): 617-26. Smith, M. L. and A. J. Fornace, Jr. (1996). 'Mammalian DNA damage-inducible genes associated with growth arrest and apoptosis.' Mutat Res 340(2-3): 109-24. Spagnuolo, G., V. D'Anto, et al. (2006). 'Effect of N-acetyl-L-cysteine on ROS production and cell death caused by HEMA in human primary gingival fibroblasts.' Biomaterials 27(9): 1803-9. Stanislawski, L., M. Lefeuvre, et al. (2003). 'TEGDMA-induced toxicity in human fibroblasts is associated with early and drastic glutathione depletion with subsequent production of oxygen reactive species.' J Biomed Mater Res A 66(3): 476-82. Subay, R. K. and M. Demirci (2005). 'Pulp tissue reactions to a dentin bonding agent as a direct capping agent.' J Endod 31(3): 201-4. Tay, F. R. and D. H. Pashley (2001). 'Aggressiveness of contemporary self-etching systems. I: Depth of penetration beyond dentin smear layers.' Dent Mater 17(4): 296-308. Touil, N., A. Elhajouji, et al. (2000). 'Analysis of chromosome loss and chromosome segregation in cytokinesis-blocked human lymphocytes: non-disjunction is the prevalent mistake in chromosome segregation produced by low dose exposure to ionizing radiation.' Mutagenesis 15(1): 1-7. Umegaki, K. and M. Fenech (2000). 'Cytokinesis-block micronucleus assay in WIL2-NS cells: a sensitive system to detect chromosomal damage induced by reactive oxygen species and activated human neutrophils.' Mutagenesis 15(3): 261-9. Vairetti, M., A. Ferrigno, et al. (2005). 'Apoptosis vs. necrosis: glutathione-mediated cell death during rewarming of rat hepatocytes.' Biochim Biophys Acta 1740(3): 367-74. Wang, B., W. Y. Feng, et al. (2006). 'Acute toxicity of nano- and micro-scale zinc powder in healthy adult mice.' Toxicol Lett 161(2): 115-23. Warheit, D. B., J. F. Hansen, et al. (1997). 'Inhalation of high concentrations of low toxicity dusts in rats results in impaired pulmonary clearance mechanisms and persistent inflammation.' Toxicol Appl Pharmacol 145(1): 10-22. Warheit, D. B. and M. A. Hartsky (1990). 'Species comparisons of proximal alveolar deposition patterns of inhaled particulates.' Exp Lung Res 16(2): 83-99. Warheit, D. B., L. H. Overby, et al. (1988). 'Pulmonary macrophages are attracted to inhaled particles through complement activation.' Exp Lung Res 14(1): 51-66. Watanabe, I. and N. Nakabayashi (1993). 'Bonding durability of photocured phenyl-P in TEGDMA to smear layer-retained bovine dentin.' Quintessence Int 24(5): 335-42. Winter,K.,D. Pagoria, et al.(2005).'The effect of antioxidants on oxidative DNA damage induced by visible-light-irradiated CQ/N,N-dimethyl-p-toluidine.' Biomaterials 26(26): 5321-9. Wu, H., K. K. Bhopale, et al. (2008). 'Ethanol-induced cytotoxicity in rat pancreatic acinar AR42J cells: role of fatty acid ethyl esters.' Alcohol Alcohol 43(1): 1-8. Yoshii, E. (1997). 'Cytotoxic effects of acrylates and methacrylates: relationships of monomer structures and cytotoxicity.' J Biomed Mater Res 37(4): 517-24. Zhang, J., H. Wang, et al. (2005). 'Comparison of short-term toxicity between Nano-Se and selenite in mice.' Life Sci 76(10): 1099-109. Ziady, A. G., C. R. Gedeon, et al. (2003). 'Minimal toxicity of stabilized compacted DNA nanoparticles in the murine lung.' Mol Ther 8(6): 948-56. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37313 | - |
dc.description.abstract | 實驗目的: 本實驗是藉由中國倉鼠卵巢細胞的體外試驗,評估並比較市售的五種牙本質黏著劑對細胞毒性及基因毒性的影響,所選擇的廠品包括3種成分為奈米填料(B:Voco Futurabond NR, C: 3M Singlebond 2, D:Dentsply Prime & Bond NT)、2種成分為傳統的非奈米填料(A:Voco Solobond M, E:Dentsply Xeno III)。
實驗材料與方法: 中國倉鼠卵巢細胞在五種牙本質黏著劑以不同濃度(1/40000-1/20, v/v, %)及陰性對照組(0.5%的DSMSO)作用下暴露24小時。細胞毒性的評估是利用細胞群落形成效率分析(CFE),並分別利用PI (40ug/ml)和DCFH-DA的染色下,利用流式細胞儀進行細胞週期及活性氧的評估。再利用Modfit軟體進行G0/G1期, S期, G2/M期和sub-G0/G1等細胞週期所佔比例的評估,並利用mean DCF fluorescence的值評估活性氧產生的程度。此外,並結合細胞質分裂阻斷之微核分析 (CBMN)輔以DAPI染色的技術下,計算凋亡細胞、微核細胞、核質間橋等細胞的數量,進行基因毒性的檢測。 實驗結果: 五種不同的牙本質黏著劑對中國倉鼠卵巢細胞產生了不同程度的細胞毒性影響。計算TC50所獲得的細胞毒性排名依序是:(D)>>(E)≧(C)>(A)>(B)。在高濃度的條件下,五種牙本質黏著劑皆會誘導sub-G0/G1值的增加。利用Modfit軟體進行細胞週期的評估,則可以看到在24小時的給藥時間下,B.C.D組出現顯著的G2/M期細胞週期停滯;A.C.D組則出現G0/G1期停滯的現象。而利用mean DCF fluorescence偵測到的活性氧含量,A,C,D,E在濃度為1/40 (%)時與其他濃度出現顯著差異;B組則在1/40 (%)和1/20(%)較其他濃度出現統計上的顯著差異。基因毒性的測試結果,誘導凋亡細胞產生的比例與細胞存活率實驗獲得一致性的結果;較高的微核頻率則發生在含有較小奈米填料的組別 (B,C,D);核質間橋的數量則伴隨微核細胞頻率的增加,也顯示增加的趨勢。核質間橋的出現一方面代表了染色體的修復,另一方面也顯示了基因受損的程度。 結論: 五種不同的牙本質黏著劑分別產生了不同程度的細胞毒性影響,這可能與牙本質黏著劑內含的各種成分有關。細胞毒性可能來自於成分中活性氧的產生,而誘導細胞凋亡和細胞週期的停滯所致。由我們的實驗結果得知,奈米填料的存在與否將不是決定毒性大小的唯一因素,但是奈米填料的出現,卻有可能提高染色體的損傷或遺失,導致細胞中微核及核質間橋的增加,而此結果我們推論奈米填料的出現可能引致進一步的基因毒性。這些結果與進一步的發現將對於臨床復型牙科在牙本質黏著劑的運用與瞭解上有實質性的幫助。 | zh_TW |
dc.description.abstract | Objectives: To compare the cytotoxicity & genotoxicity of five dentin bonding agents (DBAs), including three nano-DBAs (DBA-B:Voco Futurabond NR, DBA-C: 3M Singlebond 2, and DBA-D:Dentsply Prime & Bond NT) and two non-nano-DBAs (DBA-A:Voco Solobond M and DBA-E:Dentsply Xeno III) by using Chinese hamster ovary (CHO-K1) cells.
Methods: CHO-K1 cells were exposed to different concentrations of DBAs (1/40000-1/20, v/v, %) and negative control(DMSO) for 24 hrs. Cytotoxicity of DBAs was evaluated by colony formation efficiency. For cell cycle analysis and reactive oxygen species (ROS) production, CHO-K1 cells were treated with DBAs and then stained with propidium iodide (40ug/ml) or dichlorofluorescein–diacetate (DCFH-DA), respectively, and finally subjected for flow cytometric analysis. The percentage of cells in G0/G1, S, G2/M and sub-G0/G1 phases were determined. The mean DCF fluorescence was used to evaluate cellular ROS levels. Additionally, genotoxicity of DBAs was evaluated by CBMN & DAPI staining. Results: DBAs showed differential cytotoxicity to CHO-K1 cells. The potency of cytotoxicity was (D)>>(E)≧(C)>(A)>(B). All five DBAs induced sub-G0/G1 cell population at higher concentrations. After exposure of CHO-K1 cells to B.C.D for 24 h, marked G2/M cell cycle arrest was noted, whereas A.C.D induced G0/G1 cell cycle arrest. DBAs also induced ROS production as indicated by an increase in mean DCF fluorescence after exposure to DBAs (A,C,D,E at a concentration of 1/40 (%), and B at a concentration of 1/40(%) or higher,1/20(%)). About genotoxicity, significant increase of MN frequency & NPB was noted in DBAs(B,C,D), including composition of nano-filler. Conclusions: These five DBAs showed differential cytotoxicity to CHO-K1 cells. Cytotoxicity can be due to induction of apoptosis and arresting of cell cycles by components in DBAs and associated with ROS production. Absence or presence of nano-fillers is not the major cytotoxic factor for these DBAs, but significant increase of MNs & NPB could represent DNA loss or damage from nano-filler of DBAs. These results are useful for clinical operative restorative procedures. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T15:24:09Z (GMT). No. of bitstreams: 1 ntu-97-R94422012-1.pdf: 3076428 bytes, checksum: b806baaccf714e42bfd56176e16dc29c (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 謝誌 i
中文摘要 iii 英文摘要 v 目錄 vii 圖次 x 附錄 xi 第一章 文獻綜論 1 1.1 牙科奈米科技的發展及牙本質黏著劑在臨床上的應用 1 1.1.1 牙本質黏著劑的歷史發展暨系統的演變 1 1.1.2 牙本質黏著劑在臨床上的運用 5 1.1.3 牙科奈米科技的發展 6 1.2 不同黏著劑單體對細胞的影響 7 1.3 牙本質黏著劑(單體)對細胞週期與細胞凋亡的影響 8 1.3.1 活性氧物種(reactive oxygen species, ROS)對細胞的影響 9 1.3.2 細胞的凋亡(Apoptosis) 10 1.3.3 細胞週期的調控分子-p53 11 1.4 染色體變異試驗(in vitro chromosome aberration test) 11 1.5 基因毒性與微細胞核(Micronucle)的產生 12 1.6 細胞質分裂阻斷之微核分析技術(Cytokinesis-block micronucleus assay)..13 1.7 細胞模式實驗 14 實驗動機與目的 15 第二章 實驗材料與方法 16 2.1 實驗材料與藥品來源 16 2.2 牙本質黏著劑的濃度(v/v %)與分裝 18 2.3 細胞株與細胞培養 19 2.4 細胞存活率試驗 20 2.5 基因毒性與免疫螢光顯微鏡 (Immunofluorescence microscopy)的運用 20 2.5.1 利用DAPI染色標記細胞核的位置 20 2.5.2 CBMN methodology under DAPI stain 21 2.6 利用流式細胞技術分析細胞內活性氧(ROS)含量 22 2.7 利用流式細胞儀分析細胞週期之變化 23 2.8 統計分析 24 第三章 實驗結果 25 3.1 牙本質黏著劑在高濃度下會抑制CHO-K1 細胞的生長與造成細胞毒性 25 3.1.1 長期細胞存活率分析 ~ 細胞群落形成效率分析 25 3.1.2 長期細胞存活率分析 ~ TD50的計算 25 3.2 牙本質黏著劑在高濃度的條件下會改變CHO-K1細胞之細胞形態 26 3.3 牙本質黏着劑會造成細胞內活性氧含量的上昇 26 3.4 牙本質黏着劑對細胞週期的影響 27 3.5 牙本質黏著劑誘導的基因毒性對細胞死亡的影響 28 3.5.1 Apoptosis/necrosis 的實驗結果 30 3.5.2 微核的實驗結果分析 30 3.5.3 核質小橋的實驗結果分析 31 第四章 討論 33 4.1 不同牙本質黏着劑對細胞毒性所造成的影響 33 4.2 牙本質黏着劑引起活性氧的增加及抑制劑的使用 34 4.3 牙本質黏着劑造成細胞週期停滯的討論 35 4.4 不同牙本質黏着劑對基因毒性所造成的影響 36 4.5 單體差異與牙本質黏着劑毒性的的影響 37 4.6 牙本質黏着劑其餘組成及光聚合程度對毒性造成的影響 39 第五章 總結 41 參考文獻 42 圖 次 圖1、牙本質黏著劑滲入牙本質小管的電子顯微鏡圖片 53 圖2、特殊實驗材料化學結構式 54 圖3、DMSO所產生的細胞毒性影響 55 圖4、Bis-GMA的結構式 56 圖5、細胞存活率實驗統計圖 57 圖6、細胞存活率實驗照片 58 圖7、五種牙本質黏著劑TC50的比較 59 圖8、不同濃度牙本質黏著劑作用下細胞形態的改變 60 圖9、活性氧含量的比較 61 圖10、樣本(A)� PI染色 & 細胞週期的分析 62 圖11、樣本(B)� PI染色 & 細胞週期的分析 63 圖12、樣本(C)� PI染色 & 細胞週期的分析 64 圖13、樣本(D)� PI染色 & 細胞週期的分析 65 圖14、樣本(E)� PI染色 & 細胞週期的分析 66 圖15、Apoptosis (DAPI染色下的記數結果) 67 圖16、微核 (DAPI染色下的記數結果) 68 圖17、核質間橋 (DAPI染色下的記數結果) 69 圖18、利用ICP-MS 分析偵測內含的金屬成分 70 附 錄 附錄1、染色體受損時的修復機制 71 附錄2、DNA障礙下,錯誤修復產生NPB & MN 的方式 72 附錄3、真核細胞有絲分裂的分期 73 附錄4、細胞週期 74 附錄5、染色體染色判讀 75 附錄6、DAPI染色 (MN & NPB) 76 | |
dc.language.iso | zh-TW | |
dc.title | 比較五種傳統與奈米級牙本質黏著劑毒性機轉之探討 | zh_TW |
dc.title | Mechanisms of Cytotoxicity by Five Conventional and Nano Dentin Bonding Agents to CHO-K1 cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 林俊彬 | |
dc.contributor.oralexamcommittee | 李士元,許明倫 | |
dc.subject.keyword | 細胞凋亡,細胞週期,中國倉鼠卵巢細胞,細胞毒性,牙本質黏著劑,奈米,活性氧, | zh_TW |
dc.subject.keyword | Apoptosis,Cell cycle,CHO-K1 cells,Cytotoxicity,Dentin bonding agent,Nano-materials,Reactive oxygen species, | en |
dc.relation.page | 76 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2008-07-22 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
顯示於系所單位: | 臨床牙醫學研究所 |
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