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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 徐源泰(Yuan-Tay Shyu) | |
| dc.contributor.author | Huei-Mei Fu | en |
| dc.contributor.author | 傅惠美 | zh_TW |
| dc.date.accessioned | 2021-06-13T15:18:42Z | - |
| dc.date.available | 2009-07-30 | |
| dc.date.copyright | 2008-07-30 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-07-23 | |
| dc.identifier.citation | 參考文獻
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37070 | - |
| dc.description.abstract | 目前全球基因改造生物(Genetically Modified Organisms, GMO)發展，依然快速增長中，根據Agbios 資料庫顯示2008年全球核准上市的基因改造玉米共有44種，其中約有四分之一屬於混合型(stacked)品系的玉米，這些新品系最大的爭議在於是否須要重新評估其安全性。我國目前核准基因改造玉米品系則為單一基因改造玉米品系14種，尚未核定任何混合品系，為釐清我國目前市場可見之販售玉米與核定品系間之遵循性，以做為對基改食品管理機制的基礎，故進行此調查研究，本實驗共分成基改玉米鑑定系統與基改玉米定量系統兩部份。
基改玉米鑑定系統方面，由全臺灣共取得107個取樣點取的顆粒完整之散裝玉米樣品，由樣品中選取共321個試驗樣品，進行基改玉米篩選。35S 啟動子、nos 終止子及玉米內源性基因zein進行初篩，結果共獲得17個基因改造玉米的陽性樣品，再以Onishi等學者於2005年所發表之基改玉米鑑定系統進行品系檢定，在17個陽性樣品中，篩得9個含有基因改造成分，包含NK603品系、NK603ＸMON810混合品系、NK603ＸMON863混合品系、NK603、MON863與M0N810混合品系、MON863品系等；基改玉米定量系統方面則以以Yang等學者於2007年與Kuribara等學者於2002所發表之研究報告為基礎，配合所能取得之基改玉米標準品，建立SYBR Green I 定量系統之建立，認證標準樣品包含品項混雜比例從0~5.0%不等，經過驗證後此定量系統的偏估值變動的分布主要在± 41%以內，而變異係數變動範圍主要在33%以內，顯示此定量系統可得到可靠的定量結果。 | zh_TW |
| dc.description.abstract | Currently, there is a rapid development on Genetically Modified Organisms(GMO) globally. Fourty-four kinds of GM maize have been approved to launch to the market in 2008 according to Agbios database. Appropriraely one quarter of those 44 kinds are Stacked maize. There are many disputes on the necessity for re-evaluating the safety of these new products currently. Taiwan government has not approve amy imported Stacked maize. In order to provide a basic GM-food managing mechanism, clarifying the relationship between the maize in the market and approved GM maize lines is im-portant. This study consists of two parts, namely, GMO screening PCR system and GM maize quantitative detection.
In the GMO screening PCR system, the bulk maize samples intact which 107 sam-pling in Taiwan, and 321 test samples were chosen from the sample to select GM maize. Using 35S promoter, NOS terminator and maize endogenous gene zein to proceed the first stage of selection and we get 17 GMO positive GM maize. Then, based on the “GMO screening PCR system” published by scholars including Onishi in 2005, GMO positive maize out of 17 GMO positive samples were selected, including, NK603lines、NK603ＸMON863 stacked lines、NK603ＸMON810 stacked lines、NK603、MON863 and M0N810 stacked lines、MON863 lines. The GM maize quantitative detection is based on two published reports. Using the certified reference materials include certified reference materials ranging varies from 0~5.0% to develop real-time PCR systems, which is based on SYBR Green I technologies for specific guantitative. In this experi-ment, the bias range of quantification system has been approved within ±33%, the coef-ficients of variation distribute within 41%. These results have proved that this GMO quantification system developed in this study is reliable in practice. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T15:18:42Z (GMT). No. of bitstreams: 1 ntu-97-R95628203-1.pdf: 2134331 bytes, checksum: 0d1a26d0e4402722727701fa00c339f9 (MD5) Previous issue date: 2008 | en |
| dc.description.tableofcontents | 目錄
中文摘要............................................I 英文摘要............................................II 目錄................................................III 圖次................................................VI 表次................................................X 第一章 前言.........................................1 第二章 前人研究.....................................3 第一節 基因改造生物之簡介...........................3 一、何謂基因改造生物...............................3 二、基因改造作物之優點.............................3 三、基因改造作物發展與現況.........................4 四、基因改造生物之潛在疑慮.........................5 第二節 基因改造食品之檢測...........................11 一、基因改造食品定性與定量檢測.....................11 (1).定性分析....................................11 (2).定量分析....................................13 第三節 基因改造玉米之介紹...........................22 一、玉米簡介........................................22 二、基因改造玉米介紹...............................22 (1).耐除草劑(herbicide tolerance)基因改造玉米...22 (2).抗病蟲害(insect resistance)基因改造玉米.....23 三、玉米內源性基因 ................................. 25 第三章 材料與方法...................................29 第一節 研究材料.....................................29 第二節 藥品與試劑...................................29 第三節 實驗儀器與設備...............................31 第四節 實驗方法.....................................32 一、DNA樣品製備與純化...............................32 (1).樣品前處理..................................32 (2). DNA萃取....................................33 二、收集資料與設計PCR引子對.........................34 三、聚合酶連鎖反應(PCR).............................34 四、標準參考質體的建立..............................34 五、質體DNA之抽取...................................35 六、標準參考質體之濃度..............................35 七、定性檢測........................................35 八、即時定量聚合酶鏈鎖反應(Lightcycler定量分析).....36 第四章 結果與討論...................................50 第一節 基因改造玉米之定性檢測.......................50 一、基因改造玉米初步篩檢............................50 二、基因改造玉米品系鑑定............................51 第二節 即時聚合酶鏈鎖反應檢測.......................59 一、標準參考質體的構築.............................60 二、即時聚合酶鏈鎖反應引子專一性測試................60 三、質體標準樣品之標準曲線與檢測範圍................62 四、內標比..........................................66 五、檢測系統的正確性................................68 六、多品系基因改造玉米檢測方法之應用................69 第五章、結論........................................71 參考文獻............................................119 附錄一..............................................130 附錄二..............................................131 附錄三..............................................132 附錄四..............................................133 | |
| dc.language.iso | zh-TW | |
| dc.subject | 基改玉米定量系統 | zh_TW |
| dc.subject | 基改玉米鑑定系統 | zh_TW |
| dc.subject | 混合型品系 | zh_TW |
| dc.subject | SYBR Green I | zh_TW |
| dc.subject | 基因改造玉米 | zh_TW |
| dc.subject | GM maize | en |
| dc.subject | SYBR Green I | en |
| dc.subject | GM maize quantitative detection | en |
| dc.subject | GMO screening PCR system | en |
| dc.subject | stacked lines | en |
| dc.title | 多品系基因改造玉米檢測方法之開發 | zh_TW |
| dc.title | Development of detection methods for Multiline Genetically Modified Maize | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳昭瑩,許輔,曾文聖 | |
| dc.subject.keyword | 基因改造玉米,混合型品系,基改玉米鑑定系統,基改玉米定量系統,SYBR Green I, | zh_TW |
| dc.subject.keyword | GM maize,stacked lines,GMO screening PCR system,GM maize quantitative detection,SYBR Green I, | en |
| dc.relation.page | 128 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2008-07-25 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 園藝學研究所 | zh_TW |
| 顯示於系所單位： | 園藝暨景觀學系 | |
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