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標題: | 利用聚氯乙烯薄膜接枝雙胺仿聚離胺酸探討其對神經元細胞之影響 The Study of the Behavior of Neurons Cultured on PVC Membranes Modified With Diamines Like PDL |
作者: | Tzong-Hann Yang 楊宗翰 |
指導教授: | 楊台鴻 |
關鍵字: | 小腦顆粒神經細胞,聚氯乙烯,雙胺,離胺酸,PC12神經細胞株,細胞電泳, cerebellum granule cells,PVC,PC12 cell line,diamine, |
出版年 : | 2005 |
學位: | 碩士 |
摘要: | 本實驗是以乾式法將高分子聚氯乙烯製成薄膜之後,用化學改質的方式將表面接枝各式雙胺,來仿照聚離胺酸結構式,以討論材料表面官能基改變對於神經細胞的生長之影響。
由改質後的薄膜培養七天大大鼠的小腦顆粒神經細胞及PC12神經細胞株,並以MTT測試來比較細胞的活性,另外在掃瞄式電子顯微鏡的觀察來了解其細胞型態上的變化,最後再以細胞電泳來觀察材料與細胞表面電位的影響。 改質過後的聚氯乙烯,從較疏水的性質轉為親水性質,改質過後的聚氯乙烯薄膜對培養神經細胞均有較佳的細胞活性,且長天期培養後,趨勢也和一般的聚離胺酸相仿,證實改質後的聚氯乙烯薄膜能有效地促進神經細胞生長。 We prepared poly (vinyl chloride) (PVC) membranes by the dry processing, and then grafted diamine on the chloride group of these membrane surface by the way of chemical modification. To follow the structure of poly-D-lysine as a model and discuss the relationship between the functional groups of the modified material and the growth of neuron cells. We cultured the cerebellum granule cells of the Wistar rat and PC12 cell lines on the modified membranes and compared with the relative activity and the cell morphology by MTT assay and scanning electron microscope (SEM). In addition, the relationship between the materials and the surface electrophoretic mobility of PC12 was observed by microelectrophoresis. The modification of PVC membranes were more hydrophilic than PVC, in general, have better relative activity. Moreover, in the long period culture they could have similarly condition of lysine.The graft of diamine on the modified PVC membrane could effectively induce the growth of neuron cells. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/36909 |
全文授權: | 有償授權 |
顯示於系所單位: | 醫學工程學研究所 |
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