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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃世佑(Shih-Yow Huang) | |
| dc.contributor.author | Shing-quing Lu | en |
| dc.contributor.author | 盧秀青 | zh_TW |
| dc.date.accessioned | 2021-06-13T08:21:06Z | - |
| dc.date.available | 2005-07-27 | |
| dc.date.copyright | 2005-07-27 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-19 | |
| dc.identifier.citation | Amorini A.M., Fazzina G., Lazzarino G., Tavazzi B., Di Pierro D., Santucci R., Sinibaldi F., Galvano F., Galvano G., 2001 Activity and mechanism of the antioxidant properties of cyanidin-3-O- beta-glucopyranoside, Free Radic. Res., 35, 953-966.
Auger C., Caporiccio B., Landrault N., Teissedre P.L., Laurent C., Cros G., Besancon P., Rouanet J.M., 2001 Red wine phenolic compounds reduce plasma lipids and apolipoprotein B and prevent early aortic atherosclerosis in hypercholesterolemic Golden Syrian hamster ( Mesocricetus auratus), Nutr. Metab., 131, 2837-2842. Bomser J., Madhavi D.L., Singletary K., Smith M.A.L., 1996 In vitro anticancer activity of fruit extracts from Vaccinum species, Planta. Med., 62, 193-288. Bridle P., Timberlake C.F. 1997 Anthocyanins as natural food colours-selected aspect, Food Chem., 58, 103-109. Cemeroglu B., Velioglu S., Isik S., 1994 Degradation kinetics of anthocyanins in sour cherry juice and concentrate, J. Food Sci., 59, 1216-1221. Chen C.C., Hsu J.D., Wang S.F., Chiang H.C., Yang M.Y., Kao E.S., Ho Y.C. Wang C.J., 2003 Extract inhibits the development of atherosclerosis in cholesterol-fed rabbits, J. Agric. Food Chem., 51, 5472-5477. Choi M. H., Kim G. H., Lee H. S., 2002 Effects of ascorbic acid retention on juice color and pigment stability in blood orange(Citrus sinensis) juice during refrigerated storage, Food Res. international, 35, 753-759. da Costa C.T., Horton D., Margolis S.A., 2000 Analysis of anthocyanins in foods by liquid chromatography, liquid chromatography-mass spectrometry and capillary electrophoresis, J. Chromatogr. A, 881, 403-410. Du C.T., Francis F.J., 1973 Anthocyanins of roselle, J. Food Sci., 38, 810-812. Dugo P., Mondello L., Errante G., Zappia G., Dugo g., 2001 Identification of anthocyanins in berries by narrow-bore high-performance liquid chromatography with electrospray ionization detection, J. Agric. Food Chem., 49, 3987-3992. Frankel E.N., Waterhouse A.L., Teissedre P.L., 1995 Principal phenolic phytochemicals in selected California wines and their antioxidant activity in inhibiting oxidation of human low-density lipoproteins, J. Agric. Food Chem., 43, 890-894. Fuhrman B., Lavy A., Aviram M., 1996 Consumption of red wine with meals reduces the susceptibility of human plasma and low-density lipoprotein to lipid peroxidation, Am. J. Clin. Nutr., 61, 549-554. Fuleki T., Francis F.J., 1968 Quantitative method for anthocyanins determination of total anthocyanin and degradation index for cranberry juice, J. Food Sci., 33, 78-83. Galvano F., Fauci L. L., Lazzarino G., Fogliano V., Ritieni A., Ciappellano S., Battistini N.C., Tavazzi B., Galvano G., 2004 Cyanidins: metabolism and biological properties, J. Nutr. Biochem., 15, 2-11. Garcia-Alonso M., Rimbach G., and Rivas-Gonzalo J. C., 2004 Antioxidant and cellular activities of anthocyanins and their corresponding vitisins A-studies in platelets, monocytes, and human endothelial cells, J. Agric. Food Chem., 52, 3378-3384. Garcia-Viguera C., Bridle P., 1999 Influence of structure on color stability of anthocyanins flavylium salts with ascorbic acid, Food Chem., 64, 21-26. Garcia-Viguera C., Zafrilla P., Tomás-Barberán F.A. 1998 The use of acetone as an extraction solvent for anthocyanins from strawberry Fruit, Phytochemical Anal., 9, 274-277. Ghiselli A., Nardini M., Baldi A., Scaccini C., 1998 Antioxidant activity of different phenolic fractions separated from an Italian red wine, J. Agric. Food Chem., 46, 361-367. Hagiwara A., Miyashita K., Nakanishi T., Sano M., Tamano S., Kadota T., Koda T., Nakamura M., Imaida K., Ito N., Shirai T., 2001 Pronounced inhibition by a natural anthocyanin, purple corn color, of 2-amino-1-methyl-6-phenylimidazo[4, 5- b]pyridine (PhIP)-associated colorectal carcinogenesis in male F344 rats pretreated with 1, 2-dimethylhydrazine, Cancer Lett., 171, 17-25. Haruna A.K., 1997 Cathartic activity of soborodo: the aqueous extract of calyx of Hibiscus sabdariffa L., Phytotherapy Res., 11, 307-308. Hendry G.A.F., Houghton J.D., 1996 Natural food colorants, Blackie Academic & Professional press. Hu C., Zawistowski J., Kitts D.D., 2002 Phytochemical characterization and bioactivities of anthocyanins from pigmented rice, Annual Meeting and Food Expo – Anaheim, California. Session 84, Nutraceuticals & Functional Foods. Iris F., Benzie F., Strain J.J., 1996 The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay, Anal. Biochem., 239, 70-76. Ishikura N., 1975 A survey of anthocyanins in fruits of some angiosperm, I*. Bot. Mag. Tokyo, 88, 41-47. Jankowski A., Jankowska B., and Niedworok J., 2000 The influence of aronia melanocapra in experimental pancreatitis, Merkuriusz Lek., 48, 395-398. Jankowski A., Jankowska B., and Niedworok J., 2000 The effect of anthocyanin dye from grapes on experimental diabetes, Folia Med Cracov., 41, 5-15. Jayaprakasam B., Vareed S. K., Olson L. K., Nair M. G., 2005 Insulin by bioactive anthocyanins and anthocyanidins present in fruits, J. Agric. Food Chem, 53, 28-31. Joseph J.A., Shukitt-Hale B., Denisova N.A., Bielinski D., Martin A., McEwen J.J., and Bickford P.C., 1999 Reversals of age-related declines in neuronal signal transduction cognitive, and motor behavioral deficits with blueberry, spinach, or strawberry dietary supplementation, The Journal of Neuroscience, 19, 8114-8121. Kamei H., Hashimoto Y., Koide T., Kojima T., Hasegawa M., 1998 Anti-tumor effect of methanol extracts from red and white wines, Cancer Biotherapy and Radiopharmacology, 13, 447-452. Kanget S.Y., Seeram N.P., Nair M.G., Bourquin L.D., 2003 Tart cherry anthocyanins inhibit tumor development in ApcMin mice and reduce proliferation of human colon cancer cells, Cancer Lett., 194, 13-19. Kim M-Y., Iwai K., Onodera A., Matsue H., 2003 Identification and antiradical properties of anthocyanins in fruits of Viburnum dilatatum Thunb., J. Agric. Food Chem., 51, 6173—6177. Kondo K., Matsumoto A., Kurata H., Tanahashi H., Koga H., Amachi T., Itakura H., 1994 Inhibition of low density lipoprotein with red wine, Lancet, 344, 1152. Kong J.M., Chia L.S., Goh N.K., Chia T.F., Brouillard R., 2003 Analysis and biological activities of anthocyanins, Phytochem., 64, 923-933. Kouniaki S., Kajda P., Zebetakis I. 2004 The effect of high hydrostatic pressure on anthocyanins and ascorbic acid in blackcurrants (Ribes nigrum). Flavour. and Fragrance J., 19, 281-286. Lazze M.C., Pizzala R., Savio M., Stivala L.A., Prospei E., Bianchi L., 2003 Anthocyanins protect against DNA damage induced by tert-butyl-hydroperoxide in rat smooth muscle and hepatoma cells., Mutat. Res., 336, 103-109. Liu M., Qi Li X., Weber C., Lee C.Y., Brown J., Liu R.H., 2002 Antioxidant and antiproliferative activities of raspberries, J. Agric. Food Chem., 50, 2926-2930. Maki Z., Inamoto H., 1973 Anthocyanins of mulberry, Chem. Absor., 78, 82073-82078. Markakis Pericles, 1982 Athocyanins as food colors, Academic press (U.S.A). Matsumoto H., Inaba H., Kishi M., Tominaga S., Hirayama M., Tsuda T., 2001 Orally administered delphinidin 3-rutinoside and cyanidin 3-rutinoside are directly absorbed in rats and humans and appear in the blood as the intact forms, J. Agric. Food Chem., 49, 1546-1551. Mcghie T.K., Ainge G.D., Barnett L.E., Cooney J.M. Jensen D.J., 2003 Anthocyanin glycosides from berry fruit are absorbed and excreted unmetabolized by both humans and rats, J. Agric. Food Chem., 51, 4539-4548. Meiers S., Kemeny M., Weyand U., Gastpar R., von Angerer E., Marko D., 2001 The anthocyanidins cyanidin and delphinidin are potent inhibitors of the epidermal growth-factor receptor, J. Agric. Food Chem., 49, 958-962. Milbury P. E., Cao G., Prior R. L., Blumberg J. 2002 Bioavailability of elderberry anthocyanins, mechanisms of ageing and development., 123, 997-1006. Miyata M., Takano H., Takahashi K., Sasaki Y.F., Yamazoe Y., 2000 Suppression of 2-amino-1-methyl-6-phenylimidazo[4,5- b]pyridine-induced DNA damage in rat colon after grapefruit juice intake, Cancer Lett., 183, 17-22. Miyazawa T., Nakagawa K., Kudo M., Muraishi K., Someya K., 1999 Direct intestinal absorption of red fruit anthocyanins, cyaniding-3-glucoside and cyaidin-3, 5-diglucoside, into rats and humans, J. Agric. Food Chem., 47, 1083-1091. Morazzoni P. Vaccinium Myrtillus anthocyanosides, In Eppos – Rivista Italiana, Actes des 13èmes Journèes Internationales Huiles Essentielles. 1-3 September Digne-Les-Bains, France, 515-54. Murkovic M., Toplak H., Adam U., and Pfannhauser W., 2000 Analysis of anthocyanins in plasma for determination of their bioavailability. J. Food Composition and Anal., 13, 291-296. Mülleder U., Murkovic M. and Pfannhauser W., 2002 Urinary excretion of cyanidin glycosides, J. Biochem. Biophys. Methods., 53, 61-66. Netzel M., Strasse G., Janssen M., Bitsch I., Bitsch R., 2001 Bioactive anthocyanins detected in human urine after ingestion of blackurrant juice, J. Environ. Pathol. Toxicol. Oncol., 20, 89-95. Nielsen I.L.F. Dragsted L.O. Ravn-Haren G., Freese R., Rasmussen S.E., 2003 Absorption and excretion of black currant anthocyanins in humans and watanabe heritable hyperlipidemic rabbits, J. Agric. Food Chem., 51, 2813-2820. Nørbæk R., Kondo T., Anthocyanins from flowers of Lilium (Liliaceae), Phytochem., 50, 71181-1184. Noda Y., Kaneyuki T., Mori A., and Packer L., 2002 Antioxidant activities of pomegranate fruit extract and its anthocyanidins: delphinidin, cyanidin, and pelargonidin, J. Agric. Food Chem., 50, 166-171. Noda Y., Kaneyuki T., Igarashi K., Mori A., Packer L., 1998 Antioxidant activity of nasunin, an anthocyanin in eggplant, Res. Communication in Molecular Pathology and Pharmacology 102, 175-187. Noda Y., Kaneyuki T., Igarashi K., Mori A., Packer L., 2000 Antioxidant activity of nasunin, an anthocyanin in eggplant peels. Toxicology, 148, 119-123. Noda Y., Kaneyuki T., Igarashi K., Mori A., Packer L., 2002 Antioxidant activity of pomegranate fruit extract and its anthocyanidins: delphinidin, cyanidin, and pelargonidin, J. Agric. Food Chem., 50, 166-171. Obi F., Usenu I., Osayande J., 1998 Prevention of carbon tetrachloride-induced hepatotoxicity in the rat by H. Rosasinensis anthocyanin extract administered in ethanol, Toxicology, 131, 93-98. Pawlowicz P., Wilczynski J., Stachowiak G., Hincz P., 2000 Administration of natural anthocyanins derived from chokeberry retardation of idiopathic and preclamptic origin. Influence on metabolism of plasma oxidized lipoproteins: the role of autoantibodies to oxidize low density lipoproteins, Ginekol Pol., 71, 848-853. Piga A., Caro A.D., Pinna I., Agabbio M. 2005 Anthocyanin and colour evolution in naturally black table olives during anaerobic processing, Swiss Society of Food Science and Technology, 38, 425-429. Pouget M.P., Lejeune B., Vennat B., Pourrat A., 1990 Extraction, analysis and study of the stability of Hibiscus anthocyanins, Lebensm-Wiss. u.-Technol., 23, 103-105. Pouget M. P., Vennat B., Lejeune B., and Pourrat A., 1990 Identification of anthocyanins of Hibiscus sabdariffa L., Lebensm-Wiss. u.-Technol., 23, 101-102. Prior R., Cao G., Martin A., Sofic E., McEwen J., O’Brien C., Lischner N., Ehlenfeldt M., Kalt W., Krewer G., 1998 Antioxidation capacity as influenced by total phenolic and anthocyanin content, maturity, and variety of Vaccinium Species, J. Agric. Food Chem., 46, 2686-2693. Proteggente A.R., Pannala A.S., Paganga G., Buren L.V., Wagner E., Wiseman S., 2002 The antioxidant activity of regularly consumed fruit and vegetables reflects their phenolic and vitamin C composition, Free Radic. Res., 36, 217-233. Ramirez-Tortosa C., Andersen O.M., Gardner P.T., Morrice P.C., Wood S.G., Duthie S.J., Collins A.R., Duthie G.G., 2001 Anthocyanin-rich extract decreases indices of lipid peroxidation and DNA damage in vitamin E-depleted rats, Free Radic. Biol. Med., 31, 1033-1037. Revilla E., Ryan J., Martín-Ortega G., 1998 Comparison of several procedures used for the extraction of anthocyanins from red grapes, J. Agric. Food Chem., 46, 4592-4595. Russo A., Bonina F., Acquaviva R., Campisi A., Galvano F., Ragusa N., Vanella A., 2002 Antioxidant activity and protective effect on DNA cleavage of a red orange extract, J. Food Sci., 67, 2814-2818. Salah A.M., Gathumbi J., Vierling W., 2002 Inhibition of instestinal mobtility by methanol extracts of Hibiscus sabdariffa L. (Malvaceae) in Rats, Phytother. Res., 16, 283-285. Sarma A.D., Sreelakshmi Y., Sharma R., 1997 Antioxidant ability of anthocyanins against ascorbic acid oxidation, Phytochem., 45, 671-674. Sarma A.D., Sharma R., 1999 Anthocyanin-DNA copigmentation complex - mutual protection against oxidative damage, Phytochem., 52, 1313-1318. Satue-Garcia M.T., Heinonen M., Frankel E.N., 1997 Anthocyanins as Antioxidants on human low-density lipoprotein and lecithin-liposome systems, J. Agric. Food Chem. 45, 3362-3367. Schwarz M., Hillebrand S., Habben S., Degenhardt A., Winterhalter P., 2003 Application of high-speed countercurrent chromatography to the large-scale isolation of anthocyanins, Biochem. Eng. J., 14, 179-189. Seeram N.P., Momin R.A., Nair M.G., Bourquin L.D., 2001 Cyclooxygenase inhibitory and antioxidant cyanidin glycosides in cherries and berries, Phytomedicine, 8, 362-369. Serrraino I, Dugo L., Dugo P., and Mondello L., 2003 Protective effects of cyanidin-3-O-glucoside from blackberry extract against peroxynitrite-induced endothelial dysfunction and vascular failure, Life Sci. 73, 1097-1114. Stintzing F.C., Clarle R., 2004 Functional propertied of anthocyanins and betalains in plants, food and in human nutrition, Trends in Food Sci. and Tech., 15, 19-38. Talavera S. Felgines C., Texier O., Besson C., Gil-Izquierdo A., Lamaison J.L.. Remesy C., 2005 Anthocyanin metabolism in rats and their distribution to digestive area, kidney, and brain, J. Agric. Food Chem., 53, 3902-3908. Tedesco I., Gian L.R., Nazzaro F., Russo M., Palumbo R., 2001 Antioxidant effect of red wine anthocyanins in normal and catalase-inactive human erythrocyte, J. Nutr. Biochem., 12, 505-511. Tee P.L., Yusof S., Mohamed S., Umar N.A., Mustapha M., 2002 Effect of roselle (Hibiscus sabdariffa L.) on serum lipids of Sprague Dawley rats, Nutri. and Food Sci., 32, 190-196. Tee P. L., Yusof S., and Mohamed S., 2002 Antioxidative properties of roselle (Hibiscus sabdariffa L.) in linoleic acid model system, Nutri. and Food Sci., 32, 17-20. Timberlake C.F. Bridle P., 1980 Anthocyanins in developments in food colours (Walford, J. ed.), 1, 115-149, Applied Science Publishers Ltd., London. Torskangerpoll K. Andersen Ø.M., 2005 Colour stability of anthocyanins in aqueous solution at various pH values, Food Chem., 89, 427-440. Toscaon M.A., Lamonica G., 1975 Pigement from Morus nigra fruits, Chem. Absor., 83, 55669-55675. Toyokuni S., Itani T., Morimitsu Y., Okada K., Ozeki M., Kondo S., Uchida K., Hiai H., Tashiro T., 2001 Protective effect of colored rice over white rice on Fenton reaction-based renal lipid peroxidation in rats, Free Radic Res., 36, 583-592. Tsai P.J., McIntosh J., Pearce P., Camden B., Jordan B.P., 2002 Anthocyanin and antioxidant capacity in roselle (Hibiscus sabdariffa L.) extract, Food Res. International, 35, 351-356. Tseng T. H., Kao E. S., Chu C. Y., Chou F. P., Lin H. W., Wang C. J., 1997 Protective effects of dried flower extracts of Hibiscus sabdariffa L. against oxidative stress in rat primary hepatocytes, Food Chem. Toxicol., 35, 1159-1164. Tsuda T., Shiga K., Ohshima K., Kawakishi S., Osawa T., 1996 Inhibition of lipid peroxidation and the active oxygen radical scavenging effect of anthocyanin pigments isolated from Phaseolus vulgaris L., Biochem. Pharmacol., 52, 1033-103952. Tsuda T., Horio F., Osawa T., 1998 Dietary cyanidin 3-O- beta-D-Glucoside increases ex vivo oxidation resistance of serum in rats, Lipids, 33, 583-588. Tsuda T., Horio F., Kitoh J., Osawa T., 1999 Protective effects of dietary cyanidin 3-O-beta-D-Glucoside on liver ischemia-reperfusion injury in rats, Arch Biochem. Biophys., 368, 361-366. Tsuda T., Horio F., Kato Y., 2002 Cyanidin 3-O-beta-D-glucoside attenuates the hepatic ischemia-reperfusion injury through a decrease in the neutrophil chemoattractant production in rats, J. Nutr. Sci. Vitaminol, 48, 134-141. Tsuda T., Watanabe M., Ohshima K., Norinobu S., Choi S.W., et al., 1994 Antioxidative activity of the anthocyanin pigments cyanidin 3-O-beta-D-glucoside and cyanidin, J. Agric. Food Chem., 42, 2407-2410. Turker N., Aksay S., and Ekiz H. I., 2004 Effect of storage temperature on the stability of anthocyanins of a fermented black carrot (Daucus carota var L.) beverage: Shalgam, J. Agric. Food Chem., 52, 3807-3813. Van Sumere C.F., Van de Casteele K., De Loose R., Heursel J., 1985 In biochemistry of plant phenolics (Van Sumere C.F. and Lea P. J. eds.) 17-43, Clarendon Press, Oxford. Wang H., Cao G.H., Prior R.L., 1997 Oxygen radical absorbing capacity of anthocyanins, J. Agric. Food Chem., 2, 304-309. Wang H., Nair M.G., Strasburg G.M., Chang Y.C., Booren A.M., Gray J.I., DeWitt D.L., 1999 Antioxidant and antiinflammatory activities of anthocyanins and their aglycon, cyanidin, from tart cherries, J Nat. Prod., 62, 294-296. Wang J., Mazza G., 2002 Inhibitory effects of anthocyanins and other phenolic compounds on nitric oxide production in LPS/IFN-gamma-activated RAW 264.7 macrophages, J. Agric. Food Chem., 50, 850-857. Wong P. K., Yusof S., Ghazali H. M., Che Y. B., 2002 Physico-chemical characteristics of roselle (Hibiscus sabdariffa L.), Nutri. and Food Sci., 32, 68-73. Youdim K.A., Martin A., Joseph J.A., 2000 Incorporation of the elderberry anthocyanins by endothelial cells increases protection against oxidative stress, Free Radic. Biol. Med., 29, 51-60. Yoshimoto M., Okuno S., Yoshinaga M., Yamakawa O., Yamaguchi O., Yamada J., 1999 Antimutagenicity of sweetpotato (Ipomoea batatas) roots, Biosci. Biotecnol. Biochem., 63, 537-541. 王俊民， 2000 洛神花色素成分及其抗氧化活性之分析，中山醫學大學生物化學研究所碩士論文。 朱淯維，2003 荷葉乙醇萃取物抗氧化性之探討，國立台灣大學食品科技研究所碩士論文。 李妙真，2003 原兒茶酸及洛神花水萃取物抑制動脈粥狀硬化機轉之研究，中山醫學大學生物化學研究所博士論文。 李宜娟，2003 洛神花多酚酸及花青素萃取物對含有突變粒線體DNA之人類腫瘤細胞生長之研究，中山醫學大學生物化學研究所碩士論文。 林進財，盧煌勝，2001 臺灣桑樹之生產研究，科學農業，49，281-287。 吳靜芬，2003 桑椹花青素誘發程序性死亡及細胞週期停止之機轉，中山醫學大學生物化學研究所碩士論文。 邱思綺，2004 洛神花多酚萃取物對肝細胞麩胱甘肽轉硫酶異構元表現之探討及合併doxorubicin毒殺肝癌細胞效果之研究，中山醫學大學生物化學研究所碩士論文。 陳怡潁，2003 台灣土肉桂葉精油之組成與抗氧化性，國立台灣大學食品科技研究所碩士論文。 黃安中，2003 洛神花花青素萃取物抗氧化型低密度脂蛋白所引發動脈粥狀硬化的機轉，中山醫學大學生物化學研究所碩士論文。 曾永安，1991 洛神葵花青素之研究，國立台灣大學農業化學研究所碩士論文。 楊正憲，蔡正宗，1994 桑椹之Anthocyanins結構鑑定，食品科學，21，319-330。 劉力綱，2002 桑椹萃取物降血脂及預防動脈粥狀硬化形成之作用，中山醫學大學生物化學研究所碩士論文。 翁義宗，2003 桑椹之抗氧化及對脂多醣所誘導RAW 264.7巨噬細胞產生PGE2及NO之影響，國立中興大學食品科學系碩士論文。 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/36888 | - |
| dc.description.abstract | 本研究從新鮮桑椹果實及乾燥的洛神葵花萼粉末中萃取分離出花青素，並進行抗氧化活性測定。將樣品之甲醇粗萃取物經由快速液相層析儀(Flash chromatograph)作初步分離，以25%甲醇(含1%醋酸)及50%甲醇(含1%醋酸)溶離，再利用HPLC作定性定量分析，進一步以HPLC-PDA、LC/MS、NMR鑑定花青素之結構。鑑定結果顯示，桑椹中含有cyanidin 3-glucoside、cyanidin-3-rutinoside及pelargonidin 3- rutinoside，其中cyanidin 3-glucoside、cyanidin-3-rutinoside含量為237.0±14.26mg (100g FW)-1、158.8±8.80mg (100g FW)-1，因此每100克新鮮桑椹果實可分離出395.8±14.26mg，而乾燥洛神葵花萼粉末中，可得到花青素8.7±0.05mg/g Dry Wt.。抗氧化活性測定方面，以FRAP(Ferric Reducing Ability of Plasma)及抑制H2O2誘發老鼠腦漿脂質過氧化(Lipid peroxidation)等方法，評估甲醇粗萃取物及快速層析儀分離出的色層之抗氧化活性。在抑制脂質過氧化表現上，F1-25及F1-50的ID50(μg)為2.0、1.7，相較於Trolox的ID50 4.3μg，抑制效果相當顯著。 | zh_TW |
| dc.description.abstract | This work focuses on the separation, purification and identification of anthocyanins, mulberry (Morus spp.) and roselle (Hibiscus Sabdariffa L.) grown in Taiwan. Their antioxidative activities were also determined and compared.
Extracts from the fruit samples were fractionated by flash chromatography using 25 and 50% methanol (with 1% acetic acid each). Qualitatative and quantitative analysis of fractionated products were carried out by using HPLC and identified the structures by employing HPLC-PDA, LC-Mass as well as NMR. The results showed that the mulberry contained cyanidin 3-glucoside (237.0±14.26mg/100g FW), cyanidin 3-rutinoside (158.8±8.80mg/100g FW) and a trace amount of plargonidin 3-rutinoside. 8.7±0.05mg/g DW of anthocyanins were isolated form the dried roselle. The method of Ferric Reducing Ability of Plasma (FRAP) and the inhibition of H2O2-induced lipid peroxidation in the rat brain homogenates were employed to assess their antioxidant activities. The ID 50 values of F1-25 and F1-50 were 2.0 and 1.7 μg, respectively. Comparing to the Trolox (ID 50 4.3μg), the inhibition capability of lipid peroxidation of mulberry was remarkable | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T08:21:06Z (GMT). No. of bitstreams: 1 ntu-94-R92524020-1.pdf: 1996610 bytes, checksum: c79d4d99dea2162c6489e6a9f5b81b04 (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 誌謝…………………………………………………………………… Ⅰ
摘要……………………………………………………………………. Ⅲ Abstract………………………………………………………………… Ⅳ 目錄……………………………………………………………………. Ⅴ 圖索引………………………………………………………………… Ⅷ 表索引………………………………………………………………… Ⅹ 第一章 緒論…………………………………………………………… 1 第二章 文獻回顧……………………………………………………… 3 2.1桑椹簡介………………………………… …………………………3 2.2洛神葵之簡介……………………………………………………… 5 2.3花青素之簡介………………………………………. ……………...7 2.4花青素之結構…………… …………………………………………8 2.5影響花青素顏色與穩定度之因素……… ………………………13 2.6萃取與分析……………………… ………………………………..18 2.7花青素的代謝與分泌…………………………………… ………..19 2.8花青素的抗氧化活性……………………………………………..20 2.9花青素的生理活性………………………………………………..23 第三章 材料與方法 …………………………………………………26 3.1實驗流程……………………… …………………………………26 3.2實驗材料…………………………………………………. ……….26 3.3實驗藥品…………………………………………………. ……….26 3.4儀器裝置………………………………………………… ………..27 3.5消耗器材………………………………………………… ………..28 3.6實驗方法…………………………………………………. ……….29 3.6.1不同溶劑萃取新鮮桑椹果實的比較………………… ……….29 3.6.2還原能力的測定(FRAP)…………………………………. .…..30 3.6.3萃取………………………………………………………….. ...31 3.6.4快速層析…………………………………………………….. ...31 3.6.5高效液相層析HPLC (定性定量分析)…………………..……31 3.6.7 H2O2誘導老鼠腦部均質液脂質過氧化…………………..…..32 3.6.8快速層析儀裝置圖……………………………………………. 34 第四章 結果與討論…………… …………………………………… 35 4.1不同溶劑對新鮮桑椹果中花青素的萃取效果比較………… …..35 4.2快速層析……………………………………… …………………36 4.3以HPLC分析快速層析儀所分離出的色層…………… ………..40 4.4化合物的分析與鑑定……………………………………………. 42 4.5還原力的測定FRAP(Ferric Reducing Ability of Plasma)……… .54 4.6 H2O2誘導老鼠腦部均質液脂質過氧化………………………… 60 4.7定量分析………………………………………………………….. 64 第五章結論………………………………………… …………………..67 第六章參考文獻………………………………………… ……………68 | |
| dc.language.iso | zh-TW | |
| dc.title | 桑椹果及洛神葵中花青素之分離與純化 | zh_TW |
| dc.title | Separation and Purification of Anthocyanins of Mulberry (Morus spp.) and Roselle (Hibiscus sabdariffa L.) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 郭悅雄(Yueh-Hsiung Kuo),周綠蘋(L-P Chow) | |
| dc.subject.keyword | 花青素,桑椹,洛神葵,抗氧化活性,快速層析, | zh_TW |
| dc.subject.keyword | anthocyanin,mulberry,roselle,antioxidative activity,flash chromatography, | en |
| dc.relation.page | 79 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-07-19 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| 顯示於系所單位： | 化學工程學系 | |
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