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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳力騏 | |
| dc.contributor.author | Huan-Chang Lin | en |
| dc.contributor.author | 林煥彰 | zh_TW |
| dc.date.accessioned | 2021-06-13T08:19:52Z | - |
| dc.date.available | 2005-07-26 | |
| dc.date.copyright | 2005-07-26 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-19 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/36864 | - |
| dc.description.abstract | 以毛細管電泳技術發展出對喜樹鹼（camptothecin）及幾丁質（chitin）之去乙醯率（Degree of Deacetylation, DD）的定量分析方法。前者的分析法乃基於毛細管電泳線上微萃取的技術，可監測在萃取台灣原種青脆枝（Nothapodytes nimmoniana）中喜樹鹼的純化過程。後者乃基於硼酸根離子與胺基之間的錯合反應，此方法可用以估計幾丁物質的去乙醯率，並可應用於檢測食物中的非法添加物－硼砂。
結果顯示，利用DMSO萃取出青脆枝中的喜樹鹼溶液，可以直接注入毛細管電泳儀中做分析，此線上微萃取技術可增加樣品檢測的準確性並減少萃取過程至量測過程所需的時間。由於硼酸根離子與胺基之間的錯合反應改變了泳動率與吸光係數，促進了毛細管電泳的分離與檢測。這兩種分析方法所需的時間均在20分鐘以內。 | zh_TW |
| dc.description.abstract | Capillary electrophoresis procedures for quantifying camptothecin and glucosamine/N-acetyl glucosamine were developed. The former was based on in-capillary micro-extraction technique and can be used in monitoring the purification process of the anti-cancer agent, camptothecin, from a Taiwan medicinal tree, Nothapodytes nimmoniana. The latter was based on the complex reaction between borate ion and the aminosugars; the method is valuable for either determining deacetylation degree of chitinous material or analyzing the illegal food additive, borax.
As the result shown, the DMSO-extract of Nothapodytes nimmoniana can be directly injected, extracted and analyzed with a commercialized capillary electrophoresis system. The borate complex reaction improved the separation and detection by altering the electrophoresis mobility and the optical properties of the aminosugars. Both separation procedures were completed in less than 20 minutes | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T08:19:52Z (GMT). No. of bitstreams: 1 ntu-94-R91631018-1.pdf: 5243344 bytes, checksum: 68d7193bf44b1cc98b64962a903297ac (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 目 錄
＝＝＝＝＝＝＝＝ 口試委員簽名單(i) 序(ii) 誌謝(iii) 中文摘要(iv) 英文摘要(v) 目錄 圖目錄 表目錄 前言(1) 第ㄧ章 文獻探討(2) 1.1 毛細管電泳(2) 1.1.1 毛細管電泳之發展史(2) 1.1.2 毛細管電泳之分離原理(4) 1.1.3 電滲透流(6) 1.1.4 焦耳熱與溫度梯度(14) 1.1.5 毛細管電泳之量測方法(15) 1.1.6 毛細管電泳之限制(17) 1.1.7 毛細管電泳之影響分離效果的因素(19) 1.2 喜樹鹼(22) 1.2.1 高沸點溶媒萃取於分析上應用(24) 1.2.2 線上微萃取毛細管電泳技術(26) 1.3 幾丁物質之去乙醯率(29) 1.3.1 和尚蟹皮膜(31) 1.3.2 去乙醯率量測法簡介(35) 1.3.3 硼砂、食品添加物與殺蟲劑(37) 第二章 材料與方法(38) 第三章 結果與討論(41) 3.1 喜樹鹼的測定(41) 3.2 去乙醯率的測定(47) 第四章 結論(56) 後記(57) 參考文獻(58) 附錄一：2004年於Analytical Biochemistry所發表的論文(62) 附錄二：2004年於Journal of Food and Drug Analysis所發表的論文(66) 附錄三：九十二年生機論文發表會所發表的學會論文(70) 附錄四：九十三年生機論文發表會所發表的學會論文(78) 圖 目 錄 ＝＝＝＝＝＝＝＝ 圖1-1 毛細管兩種不同的材質之比較(4) 圖1-2 毛細管內Si-OH基解離之情形(5) 圖1-3 為毛細管電泳裝置圖(6) 圖1-4 管壁內電雙層示意圖及ζ電位(7) 圖1-5 電雙層與溶液中粒子的微觀圖(7) 圖1-6 電滲透流與層流的差異圖(9) 圖1-7 電滲透流使訊號更加明顯(10) 圖1-8 毛細管中電滲透流與樣品之示意圖(12) 圖1-9 毛細管內電解質溶液造成電流分佈的溫度梯度圖(14) 圖1-10 毛細管電泳之檢測示意圖(16) 圖1-11 50μm、75μm兩種毛細管其偵測示意圖(16) 圖1-12 毛細管電層析(CEC)之示意圖(18) 圖1-13 青脆枝之果實照片(23) 圖1-14 高沸點溶媒對喜樹鹼溶解度之比較(24) 圖1-15 醬油未經線上萃取過程之電泳圖(27) 圖1-16 醬油線上萃取過程之示意圖(27) 圖1-17 醬油經線上萃取過程之電泳圖(28) 圖1-18 幾丁質與幾丁聚醣之化學結構式(30) 圖1-19 和尚蟹於台灣西岸溼地分佈圖(31) 圖1-20 新竹香山濕地之和尚蟹照片(32) 圖1-21 經酸鹼處理過之和尚蟹幾丁皮膜(32) 圖1-22 和尚蟹皮膜應用於酵素固定之裝置示意圖(33) 圖1-23 幾丁質皮膜應用於工作電極之反應示意圖(34) 圖1-24 UV與NMR所量測去乙醯率之比較圖(36) 圖 3-1 DMSO的光譜分布圖(42) 圖 3-2 DMSO當作baseline的camptothecin溶液光譜分布圖(43) 圖 3-3 1 mg/ml camptothecin之電泳圖(44) 圖 3-4 0.1 mg/ml camptothecin之電泳圖(45) 圖 3-5 0.05 mg/ml camptothecin之電泳圖(46) 圖 3-6 Glucosamine與N-acetyl glucosamine溶於水之電泳圖(48) 圖 3-7 Glucosamine泡製2天後顏色變化（無 | |
| dc.language.iso | zh-TW | |
| dc.subject | 毛細管電泳 | zh_TW |
| dc.subject | 幾丁質 | zh_TW |
| dc.subject | 幾丁聚醣 | zh_TW |
| dc.subject | 喜樹鹼 | zh_TW |
| dc.subject | camptothecin | en |
| dc.subject | capillary electrophoresis | en |
| dc.subject | chitin | en |
| dc.subject | chitosan | en |
| dc.title | 以毛細管區帶電泳法分析幾丁聚醣品質與青脆枝中喜樹鹼含量 | zh_TW |
| dc.title | Quality Analysis of Chitosan and Nothapodytes foietida by Capillary Zone Electrophoresis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張鴻民,陳世銘,周楚洋 | |
| dc.subject.keyword | 毛細管電泳,幾丁質,幾丁聚醣,喜樹鹼, | zh_TW |
| dc.subject.keyword | capillary electrophoresis,chitin,chitosan,camptothecin, | en |
| dc.relation.page | 81 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-07-19 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 生物產業機電工程學研究所 | zh_TW |
| 顯示於系所單位： | 生物機電工程學系 | |
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