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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃耀輝,張靜文 | |
| dc.contributor.author | Wan-Yun Cheng | en |
| dc.contributor.author | 鄭宛芸 | zh_TW |
| dc.date.accessioned | 2021-06-13T07:59:35Z | - |
| dc.date.available | 2005-08-03 | |
| dc.date.copyright | 2005-08-03 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-22 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/36402 | - |
| dc.description.abstract | Legionella. pneumophila在自然界中分佈甚廣，建築體中也容易大量孳生，尤其辦公大樓、工業場所冷卻水塔與醫療院所冷熱水系統，都是L. pneumophila的可能滋生源，因此以控制水中L. pneumophila的生長，降低感染可能性，是防止退伍軍人症感染的主要方法
本研究建立加熱及加氯殺菌系統，並進行殺菌效能評估。結果顯示，L. pneumophila在60℃與0.5 mg/L 自由餘氯劑量下皆快速失去可培養力。低劑量(0.5 mg/L) 自由餘氯可使細胞膜完整之細菌減低至5 %以下，且總蛋白質大量減少。然而高溫殺菌後仍有5-40 %細菌保持細胞膜完整且總蛋白質量不受影響。當溫度又回復至37℃後，細胞膜完整之細菌比率亦有上升的可能，加氯殺菌後則無此現象。針對蛋白質表現分析，加熱殺菌後與壓力抵抗相關之蛋白質的表現量上升，尤其侵犯宿主之相關蛋白質在長時間加熱後表現量上升，代表細菌在高溫加熱後仍然存活，且欲以感染宿主作為環境壓力抵抗之可能機制。 上述結果顯示加氯殺菌對L. pneumophila控制效果優於加熱殺菌。根據目前的研究結果，建議在控制非飲用水時採用加氯法，而對熱水供應系統以加熱法為控制時，另需定期以不同指標監控環境中L. pneumophila分佈情形，以達到防治退伍軍人症的目的。 | zh_TW |
| dc.description.abstract | Legionella pneumophila is widely spread in both natural and man-made environment. The cooling tower of office buildings and the drinking water of hospitals are both likely to habit L. pneumophila. In order to protect the workers from L. pneumophila infection, it is necessary to eliminate or inhibit the growth of L. pneumophila in aquatic environment.
In this study, we evaluated the effectiveness of heat and chlorine disinfection. The culturability of L. pneumophila was lost rapidly under the disinfection condition of 60℃ and 0.5 mg/L free chlorine. The membrane integrant cell percentages were reduced down to 5 % when treated with 0.5 mg/L free chlorine and protein mass was reduced substantially. However, 5~40% cells remained membrane integrant after heat disinfection at 60℃ and 70℃ for 30 min while protein mass was not affected. As incubation temperature back to 37℃, there is a possibility for percentage of membrane integrant cells to increase after heat disinfection. In contrast, no similar observations were found in chlorine-disinfected L. pneumophila. As for protein profile of the heat-disinfected L. pneumophila, host-invasion related proteins were particularly up-regulated in 24 hr heating, implying the survival and infectious potential for the long-term heat disinfected L. pneumophila. Based on the above results, chlorination is better than heating terms of the disinfection efficiency on L. pneumophila. To control L. pneumophila in environments, it is suggested to apply chlorination as the disinfection strategy for the non-potable water system. The route surveillance with non-culture assay methods (e.g., Polymerase Chain reaction) should be performed for L. pneumophila examination in the hot water supply system when the heat treatment is served as the control method. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T07:59:35Z (GMT). No. of bitstreams: 1 ntu-94-R92841006-1.pdf: 2821738 bytes, checksum: 5663f738fa2d09da0e784abbc052e3e6 (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 摘 要 I
ABSTRACT II CONTENTS III LIST OF TABLES V LIST OF FIGURES VI LIST OF TABLES IN APPENDIX VII LIST OF FIGURES IN APPENDIX VIII A. INTRODUCTION 1 1. L. PNEUMOPHILA AND LEGIONNAIRES’ DISEASE 1 1.1. L. PNEUMOPHILA 1 1.2. LEGIONNAIRES’ DISEASE 1 2. CONTROL OF L. PNEUMOPHILA 2 2.1. OFFICIAL SUGGESTIONS 2 2.2. HEAT 3 2.3. CHLORINATION 4 3. STARVATION 5 4. EVALUATION METHODS OF DISINFECTION EFFICIENCY 5 4.1. CULTURABILITY 5 4.2. MEMBRANE INTEGRITY 6 4.3. PROTEIN MASS 6 4.4. PROTEIN PROFILE 6 B. PURPOSE 11 C. STUDY DESIGN 12 D. MATERIAL AND METHOD 15 1. BACTERIAL STRAIN AND STARVED CONDITION 15 2. HEAT TREATMENT ON STARVED L. PNEUMOPHILA 15 3. CHLORINE TREATMENT ON STARVED L. PNEUMOPHILA 16 4. CULTURABILITY OF L. PNEUMOPHILA 17 5. MEMBRANE INTEGRITY OF L. PNEUMOPHILA 17 6. DATA ANALYSIS FOR MEMBRANE INTEGRITY 19 6.1. PARAMETERS DERIVED 19 6.2. STATISTICAL ANALYSIS 20 7. PROTEOME ANALYSIS 20 7.1. Sample preparation and protein quantification 20 7.2. 2-DE 22 7.3. Image analysis 23 7.4. In-gel digestion 24 7.5. Peptide mass fingerprinting 24 7.6. Data analysis 25 E. RESULT 26 1. HEAT TREATMENT ON L. PNEUMOPHILA 26 1.1. Effect of starvation on L. pneumophila 26 1.2. The culturability of heat treated L. pneumophila 27 1.3. Total cell concentration and membrane integrity of heat treated L. pneumophila 27 1.4. The influence of 7-day incubation on L. pneumophila after heat treatment 29 2. CHLORINE TREATMENT ON L. PNEUMOPHILA 39 2.1. Chlorine concentration 39 2.2. Starvation effect on L. pneumophila at concentration of 106 – 107 cell/mL 40 2.3. Culturability of starved L. pneumophila after chlorine treatment 41 2.4. Membrane integrity of starved L. pneumophila with chlorination 41 3. PROTEOME 50 3.1. Protein concentration and mass 50 3.2. Protein expression of heat-treated L. pneumophila 50 3.3. Protein profiles of 62 qualified spots 52 F. DISCUSSION 67 1. STARVATION 67 2. HEAT DISINFECTION ON STARVED L. PNEUMOPHILA 69 2.1. Efficacy of heat disinfection on culturability of L. pneumophila 69 2.2. Heat injury on starved L. pneumophila with respect to membrane integrity 70 2.3. 7-day post-incubation after heating 73 2.4. Indicators of efficacy of heat disinfection on starved L. pneumophila 73 3. CHLORINE DISINFECTION ON STARVED L. PNEUMOPHILA 75 3.1. Efficacy of chlorine disinfection on culturability of L. pneumophila 75 3.2. Membrane injury caused by chlorine on starved L. pneumophila 76 3.3. Indicators of efficacy of chlorine disinfection on starved L. pneumophila 78 4. COMPARISON BETWEEN HEAT DISINFECTION AND CHLORINE DISINFECTION ON STARVED L. PNEUMOPHILA 79 5. PROTEIN PROFILE OF HEAT DISINFECTION L. PNEUMOPHILA 80 5.1. Metabolism related protein 80 5.2. Replication & Translation related protein 82 5.3. ATP synthesis related protein 82 5.4. Stress resistance protein 83 6. OFFICIAL SUGGESTIONS IN COMPARISONS WITH OUR RESULT 88 G. CONCLUSION 90 H. FUTURE WORK 93 I. APPENDIX 94 1. WATER BATH STABILITY MONITOR 94 2. WATER TEMPERATURE MEASUREMENT DURING HEAT TREATMENT 95 3. HACH POCKET METER VALIDITY 96 4. DETERMINATION OF INITIAL CONCENTRATION OF L. PNEUMOPHILA FOR CHLORINE TREATMENT 98 5. VALIDITY OF BACLIGHT STAIN 100 6. VOLUME OF BACLIGHT STAIN FOR CHLORINE-TREATED SAMPLES 102 7. DETERMINATION OF MINIMAL NUMBER OF COUNTING FIELDS BY FLUORESCENCE MICROSCOPY WITH BACLIGHT STAIN 103 8. LUCIA COUNTING ADJUSTMENT FOR FREE CHLORINE TREATMENT 105 9. PROTEOMIC PARAMETER SELECTION 108 J. REFERENCE LIST 114 | |
| dc.language.iso | en | |
| dc.subject | 加氯殺菌 | zh_TW |
| dc.subject | 嗜肺性退伍軍人菌 | zh_TW |
| dc.subject | 加熱殺菌 | zh_TW |
| dc.subject | chlorine disinfection | en |
| dc.subject | L. pneumophila | en |
| dc.subject | heat disinfection | en |
| dc.title | 嗜肺性退伍軍人菌加熱與加氯殺菌效能評估 | zh_TW |
| dc.title | Evaluation of heat and chlorine disinfection efficiency on Legionella pneumophila | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 潘子明,張上淳 | |
| dc.subject.keyword | 嗜肺性退伍軍人菌,加熱殺菌,加氯殺菌, | zh_TW |
| dc.subject.keyword | L. pneumophila,heat disinfection,chlorine disinfection, | en |
| dc.relation.page | 121 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-07-22 | |
| dc.contributor.author-college | 公共衛生學院 | zh_TW |
| dc.contributor.author-dept | 職業醫學與工業衛生研究所 | zh_TW |
| 顯示於系所單位： | 職業醫學與工業衛生研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-94-1.pdf 未授權公開取用 | 2.76 MB | Adobe PDF |
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