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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 劉春櫻(Chuen-Ying Liu) | |
| dc.contributor.author | Yi-Chin Sung | en |
| dc.contributor.author | 宋易瑾 | zh_TW |
| dc.date.accessioned | 2021-06-13T07:57:17Z | - |
| dc.date.available | 2006-07-28 | |
| dc.date.copyright | 2005-07-28 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-24 | |
| dc.identifier.citation | (1) Turiel, E., Esteban, A.M. J. Sep. Sci. 2005, 28, 719.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/36331 | - |
| dc.description.abstract | I
本研究以9-乙基腺嘌呤(9-ethyladenine)與tri-O-acetyladenosine 為分 子模版，用於製備整體式分子烙印聚合物管柱。其製備方法為將分子模 版、單體、交聯劑與progen 的混合溶液導入預先以γ-MPS 矽烷化之內 徑75 µm 毛細管，在聚合起始劑催化下聚合，接著利用針筒通入甲醇與 醋酸之混合溶液萃取分子模版。將所製備的管柱分別應用於核苷酸鹼 基、核苷以及核苷酸混合物的分離。對於核苷酸鹼基，在不同pH 值(4~9) 磷酸緩衝溶液，其滯留順序為cytosine > thymine > guanine > adenine，結 果顯示孔洞對adenine 有較強的滯留力，guanine 次之，thymine 與cytosine 較無明顯的滯留。核苷酸鹼基混合物最佳化分離條件為分離電壓15 kV，磷酸緩衝溶液(10 mM, pH 4)，相同管柱連續注入4 次其滯留時間的 RSD 值< 4% ，平均理論板數為3165 m-1。繼而應用於核苷混合物的分 離，在pH 值(7~8)時，核苷所帶的有效電荷接近，故adenosine 滯留較 顯著。核苷混合物最佳化分離條件為分離電壓15 kV，磷酸緩衝溶液(10 mM, pH 8)，相同管柱連續注入4 次其滯留時間RSD 值< 2.5%，平均理 論板數12169 m-1。對於核苷酸混合物，在磷酸緩衝溶液pH 值(3~5)時， 其滯留順序與有效電荷順序ㄧ致，得知AMP 並無特殊滯留，故本研究 進而以tri-O-acetyladenosine 為分子模版，在10 mM、pH 8 之磷酸緩衝 溶液AMP 有明顯滯留，但同為嘌呤類鹼基之GMP 其滯留力較弱，其最 II 佳分離條件pH 8、10 mM 之磷酸緩衝溶液，分離電壓為-25 kV，相同管 柱連續注入4 次其滯留時間RSD 值< 3.5 %，平均理論板數5039 m-1。 利用上述所建立系統為探討其複雜基質樣品的可行性，本研究選用主要 成份為多醣體並含少量核苷酸鹼基及核苷的市售靈芝膠囊作分析，以 9-ethyladenine 為分子模版所製備管柱，在硼酸緩衝溶液(10 mM, pH 9) 電壓15 kV 的環境下，成功的分離靈芝內的uracil、uridine 與adenosine， 其滯留順序為uracil > uridine > adenosine。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-13T07:57:17Z (GMT). No. of bitstreams: 1 ntu-94-R92223039-1.pdf: 4812627 bytes, checksum: 8c401fc2c56a642bb26a4fbcc7f5b7b3 (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 摘要……………………………………………………………………..Ⅰ
目錄……………………………………………………………………..Ⅲ 圗目錄…………………………………………………………………..Ⅷ 表目錄………………………………………………………………....X 第一章 序論…………………………………………………………....1 第一節 分子烙印聚合物…………………………………………..1 1.1 簡介……………………………………………………….1 1.2 分子烙印聚合物之應用………………………………….2 1.3 毛細管電層析管柱種類………………………………….2 1.3.1 填充式管柱……………………………………………..3 1.3.2 整體式管柱……………………………………………. 7 1.3.3 開管式管柱……………………………………………..8 1.5 以腺嘌呤衍生物為分子模板應用於分子烙印聚合物...10 1.6 研究動機………………………………………………...12 第二節 毛細管電層析簡介………………………………………14 2.1 前言……………………………………………………...14 2.2 毛細管電層析原理……………………………………...15 2.3 毛細管電層析管柱種類介紹…………………………...18 2.3.1 填充式管柱…………………………………………..19 2.3.2 開管式毛細管電層析………………………………..20 2.3.3 整體式毛細管電層析………………………………..21 1. 水溶性單體與分散法製備聚合物………………….21 2. 有機多孔性聚合物………………………………….22 (1) Acrylamide-based monoliths……………………….22 (2) Polystyrene-based monolithic capillary columns…..23 (3) Methacrylate ester-based monolithic columns……..24 第三節 分析物介紹……………………………………………..25 3.1 核酸…………………………………………………….25 3.1.1 核甘酸鹼基…………………………………………26 3.1.2 核苷…………………………………………………26 3.1.3 核苷酸………………………………………………27 3.2 靈芝…………………………………………………….27 第二章 實驗部份……………………………………………………..34 第一節 儀器部分…………………………………………………34 第二節 藥品部分…………………………………………………37 第三節 分子模板的合成與鑑定…………………………………39 3.1 分子模板的合成………………………………………...39 3.1.1 9-乙基腺嘌呤分子模板之合成………………………39 3.1.2 tri-O-acetyladenosine分子模版之合成….....................39 3.2 分子模板性質的鑑定.....................................................40 3.2.1 核磁共振光譜鑑定......................................................40 第四節 毛細管靜相製備與鑑定..................................................41 4.1 毛細管內部表面前處理................................................41 4.2 毛細管內壁表面矽烷基衍生化.................................. 41 4.3 毛細管內聚合反應...................................................... 41 4.3.1 9-乙基腺嘌呤分子模板之聚合……………………41 4.3.2 tri-O-acetyladenosine分子模版之聚合…................42 第五節 毛細管電層析的操作....................................................43 5.1 藥品製備.....................................................................43 5.2 毛細管電層析操作條件.............................................44 第三章 結果與討論..............................................................................48 第一節 分子模版之製備與鑑定....................................................48 1.1 分子模版之製備...............................................................48 1.1.1 9-乙基腺嘌呤之合成..................................................48 1.1.2 Tri-O-acetyladenosine..................................................49 1.2 分子模版之鑑定...............................................................49 第二節 毛細管製備與鑑定............................................................53 2.1 毛細管內部前處理...........................................................53 2.2 毛細管內部矽烷基衍生化...............................................53 2.3 毛細管內靜相聚合物的製備...........................................54 2.3.1 以9-乙基腺嘌呤為分子模板製備整體式管柱..........54 2.3.2 以tri-O-acetyladenosine為分子模板製備整體式 . 管柱..............................................................................56 2.4 毛細管電滲流測試..........................................................57 2.5 掃描式電子顯微鏡鑑定..................................................58 第三節 毛細管電層析之分析應用……………………………..65 3.1 核苷酸鹼基之分離…………………………………….65 3.2 核苷之分離…………………………………………….69 3.3 核苷酸之分離………………………………………….76 3.4 真實樣品之分析……………………………………….80 第四章 結論…………………………………………………………113 參考文獻………………………………………………………………115 | |
| dc.language.iso | zh-TW | |
| dc.subject | 分子烙印聚合物 | zh_TW |
| dc.subject | molecularly imprinted polymer | en |
| dc.title | 以腺嘌呤衍生物為分子模板之整體式分子烙印聚合物管柱的製備及應用 | zh_TW |
| dc.title | Monolithic Column with Adenine Derivatives as Template of Molecularly Imprinted Polymer
for CEC Separation | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 吳劍侯(Chien-Hou Wu),陳明娟(Min-Jane Chen) | |
| dc.subject.keyword | 分子烙印聚合物, | zh_TW |
| dc.subject.keyword | molecularly imprinted polymer, | en |
| dc.relation.page | 120 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-07-24 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 化學研究所 | zh_TW |
| 顯示於系所單位： | 化學系 | |
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