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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/36230完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳宏文 | |
| dc.contributor.author | Shu-Jui Yang | en |
| dc.contributor.author | 楊書睿 | zh_TW |
| dc.date.accessioned | 2021-06-13T07:54:24Z | - |
| dc.date.available | 2008-07-28 | |
| dc.date.copyright | 2005-07-28 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-25 | |
| dc.identifier.citation | A.J.G.Po¨tgens, S.Drewlo, M.Kokozidou and P.Kaufmann. (2004) Syncytin: the major regulator of trophoblast fusion? Recent developments and hypotheses on its action. Human Reproduction Update, 10, 487–496.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/36230 | - |
| dc.description.abstract | 利用資料分析比對人類基因體序列，可發現有兩個相關於人類內生性反轉錄病毒（human endogenous retrovirus, HERV）家族的膜蛋白質：其中一個是屬於 HERV-W 家族，也稱為 syncytin；另一個則是屬於 HERV-FRD 家族，被命名為 syncytin 2。這兩個基因被發現僅表現在胎盤，特別是胎盤的滋養層細胞，滋養層在囊胚的著床以及發育成為母體與胎兒之間的胎盤，扮演著舉足輕重的角色。我們便想針對其中具有融合功能的蛋白質 syncytin 2 進行研究。
首先，我們製備具有專一性的 syncytin 2 抗體，並且與 syncytin 抗體一起對子癇前症（pre-eclampsia）病人的胎盤組織進行西方墨點法分析，再以相同週數的正常人組織相互比較，發現 syncytin 與 syncytin 2 的蛋白質表現量在子癇前症病人之中明顯少於正常人組織。 為了進一步探討 syncytin 與 syncytin 2 的結構與功能上的關係，我們將兩蛋白質的 SU 以及 TM 次單元互相交換，而後利用 SU1–TM2 鑲嵌蛋白質（chimeric protein）進行分析，發現此鑲嵌蛋白質都可在細胞內進行水解成為 SU 與 TM 次單元，但卻無法促使融合的發生，推測 SU 與 TM 兩部分存在著某種交互作用以維持其正常功能。 我們也發現了在胎盤細胞株：BeWo、JEG3、JAR，三者之中只有 BeWo 與 JEG3 可進行自發性的細胞融合，而 JAR 細胞卻缺失了細胞融合的能力。我們排除了 JAR 細胞中扮演水解 syncytin 與 syncytin 2 的水解酶 furin 或是受器方面上的缺失。並且提出可能在 JAR 細胞中的 syncytin 與 syncytin 2 基因序列發生了突變，導致 JAR 細胞株缺失了促使細胞融合的能力。 | zh_TW |
| dc.description.abstract | Data mining of the human genome sequence has revealed two human endogenous retrovirus (HERV) envelope proteins: one is HERV-W, also called syncytin and the other is HERV-FRD named syncytin2. These two genes appear to be almost exclusively expressed in placenta, in particular, in trophoblasts that are the specialized cells of the placenta playing a major role in implantation and formation of the maternal-fetal interface. In the current study, we characterize the fusogenic function of syncytin2.
First, we induced syncytin2 antibody and then using this antibody and syncytin antibody to screen pre-eclamptic placenta and gestation age-matched controls. We found that the protein level of syncytin and syncytin2 in pre-eclampsia patient is decreased compared with the controls. We further performed domain swapping analysis and constructed chimeric protein, SU1-TM2. We found that both SU1-TM2 can be processing into SU and TM domains. On the observation of fusion assay, chimeric protein, SU1-TM2, can not mediated cell-cell fusion and propose that SU1-TM2 lost some kinds of interaction between. SU and TM domains We also studied cell fusion in three placental cell lines, BeWo, JEG3, and JAR. BeWo and JEG3 cells underwent spontaneous cell fusion, but not JAR cells. We investigated the possible mechanisms underlying the loss of spontaneous cell fusion in JAR. We ruled out the possibilities of furin processing and receptor deficiency in JAR cells and proposed that mutation in syncytin and syncytin2 genes in JAR cell line is a possible reason attributed to the loss of spontaneous cell fusion. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T07:54:24Z (GMT). No. of bitstreams: 1 ntu-94-R92b46016-1.pdf: 7655804 bytes, checksum: 4919aa140565de6b71969ccf43ab8d68 (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 目錄
目錄………………………………………………………………………....……............. I 圖表目錄........................................................................................................................... III 縮寫表.............................................................................................................................. IV 中文摘要…………………………………………………………………...............…… V 英文摘要 Abstract……………………………………………………………............. VI 第一章 緒論……………………………………………………………...............……. 1 1-1 胎盤……………………………………………………………………............. 1 1-2 胎盤的發育……………………………………………………………............. 1 1-3 細胞滋養層（trophoblast）的發育…………………………………...........…. 3 1-4 融合……………………………………………………………………............. 3 1-5 滋養層細胞的融合機制及其調控……………………………………............. 5 1-6 人類內生性反轉錄病毒（HERV）家族……………………............……...… 6 1-7 syncytin & syncytin 2…...................................................................................... 7 1-8 研究動機及目標………………………………………………………............. 9 第二章 材料與方法…………………………………………………..........…………. 10 第三章 實驗結果………………………………………………………..............……. 21 3-1 syncytin 與 syncytin 2 的一級氨基酸序列比較……………………............ 21 3-2 syncytin 與 syncytin 2 在 293T 細胞中的生物活性………....……............ 21 3-3 在大腸桿菌株 BL21（DE3）中表現 syncytin 2 之抗原……....…..........…. 23 3-4 syncytin 與 syncytin 2 之多株抗體具有專一性………………....….............. 23 3-5 syncytin 與 syncytin 2 含量在正常人與 pre-eclampsia 病人的胎盤組織中之差異…………………….………………………………….................……….… 24 3-6 鑲嵌蛋白質（chimeric protein）的生物活性………………………......…… 25 3-7 胎盤細胞株的自發性融合…………………………………………......……. 26 3-8 胎盤細胞株 JAR 可能具有 syncytin 與 syncytin 2基因序列上的缺失…. 26 第四章 討論…………………………………………………………………….......... 28 4-1 比較 syncytin 與 syncytin2 的一級胺基酸結構…………………......…… 28 4-2 子癇前症（pre-eclampsia）………………………………………….........… 29 4-3 鑲嵌蛋白質（chimeric protein）…………………………………….........… 30 4-4 胎盤細胞株JAR 缺失融合能力…………………………………….........… 31 4-5 融合機制的推測………………………………………………………......…. 31 第五章 圖表................................................................................................................... 33 第六章 參考文獻………………………………………………………......…………. 54 圖表目錄 《圖一》人類的胎盤結構................................................................................................. 33 《圖二》人類囊胚著床的過程......................................................................................... 34 《圖三》囊胚著床後的步驟............................................................................................. 35 《圖四》floating villi 和 anchoring villi 的分部............................................................ 36 《圖五》滋養層細胞的分化與功能............................................................................... 37 《圖六》胎盤絨毛的結構............................................................................................... 38 《圖七》人類內生性反轉錄病毒基因的結構............................................................... 39 《圖八》包膜蛋白質的結構........................................................................................... 40 《圖九》包膜蛋白質調控細胞融合的模式.................................................................... 41 《圖十》人類 syncytin 蛋白質的一級結構與疏水性程度圖...................................... 42 《圖十一》人類 syncytin2 蛋白質的一級結構與疏水性程度圖................................ 43 《圖十二》syncytin 與 syncytin 2 的一級胺基酸序列 alignment............................. 44 《圖十三》人類 syncytin 與 syncytin 2 蛋白質造成 293T 細胞的融合.................. 45 《圖十四》不同基因片段之 syncytin 2 在大腸桿菌中的表現與純化....................... 46 《圖十五》syncytin 與 syncytin 2 之多株抗體具有專一性........................................ 47 《圖十六》syncytin 與 syncytin 2 含量在 pre-eclampsia 與正常人之間的差異........ 48 《圖十七》鑲嵌蛋白的生物活性................................................................................... 49 《圖十八》BeWo、JAR、JEG3 與 293T 進行 co-culture assay.................................. 50 《圖十九》syncytin 與 syncytin 2 在 JAR 細胞中可能發生突變.............................. 51 《圖二十》鑲嵌蛋白質之探討....................................................................................... 52 =============================================================== 《註一》heptad repeats..................................................................................................... 53 《表一》表現於胎盤的人類內生性反轉錄病毒基因................................................... 53 | |
| dc.language.iso | zh-TW | |
| dc.subject | 融合 | zh_TW |
| dc.subject | 滋養層 | zh_TW |
| dc.subject | 胎盤 | zh_TW |
| dc.subject | syncytin 2 | en |
| dc.subject | trophoblast | en |
| dc.subject | placenta | en |
| dc.title | 胎盤融合性蛋白質 syncytin 2 的功能探討 | zh_TW |
| dc.title | Functional characterization of the placental fusion protein, syncytin 2 | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李明亭,張震東,張功耀,繆希椿 | |
| dc.subject.keyword | 滋養層,胎盤,融合, | zh_TW |
| dc.subject.keyword | syncytin 2,placenta,trophoblast, | en |
| dc.relation.page | 62 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-07-25 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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