菇蕈多醣體對四氯化碳誘導大白鼠肝損傷之修復效果評估

Abstract

本研究採用菇蕈多醣體 (mushroom β-Glucan) 為實驗材料，探討其對於四氯化碳 (CCl4) 誘導處理之大白鼠慢性肝損傷的影響。結果顯示大白鼠的體重變化率在三組實驗組與正對照組中，均比負對照組高且有顯著性差異 (p<0.05)；肝、腎臟器重量變化方面，負對照組明顯較高 (p<0.05)；在血清生化指標glutamate oxaloacetate transaminase (GOT) 與glutamate pyruvate transaminase (GPT) 方面，三種劑量多醣體處理組與正對照組，均比負對照組有顯著性的下降 (p<0.05)；肝臟蛋白質含量在負對照組有顯著性的下降 (p<0.01)，中、高劑量的多醣體處理組蛋白質含量則有顯著性的增加 (p<0.01)；在抗氧化酵素與物質方面，多醣體處理組均顯著的提升superoxide dismutase (SOD)、total sulfhydryl groups (TSH)、non-protein sulfhydryl groups (NPSH) 、protein sulfhydryl groups (PSH)、glutathione peroxidase (GPx) 、catalase (CAT) 活性與含量 (p<0.01)，並且具有劑量反應 (dose-dependent)。在肝均質液脂質過氧化程度方面，除了低劑量多醣體與負對照組沒有顯著性差異外 (p>0.01)，中、高劑量多醣體處理組和正對照組均與負對照組有顯著性差異 (p<0.01)。

This study attempts to investigate the effect of mushroom β-Glucan on the function of liver in experimental rats following the treatment of carbon tetrachloride (CCl4). The results, demonstrated that the body weight of three experimental groups and the positive control group were significantly (p<0.05) higher than that of the negative control rats. Comparatively, the liver and kidney weights of the negative control group were higher (p<0.05) than the other groups. The results showed that when β-Glucan and silymarin were used, biochemical values of liver including glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) were significantly lower (p<0.05) than those derived from the positive rats. In contrast, liver protein in the β-Glucan and silymarin treated rats was significantly higher (p<0.01) than that of the CCl4 treated animals. The present experiment also demonstrated that the activities of superoxide dismutase (SOD), total sulfhydryl groups (TSH), non-protein sulfhydryl groups (NPSP), protein sulfhydryl groups (PSH), glutathione peroxidase (GPx) and catalase (CAT) in the experimental rats were significantly (p<0.01) upgraded when β-Glucan and silymarin were used. In the experiment performed on the detection of lipid peroxidation in liver homogenate revealling that only medium and high dosage of β-Glucan treated and silymarin treated animals obtained a significant (p<0.01) activity comparing with the negative animals.