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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 齊肖琪 | |
| dc.contributor.author | Yu-Hsuan Kai | en |
| dc.contributor.author | 蓋玉軒 | zh_TW |
| dc.date.accessioned | 2021-06-13T07:47:41Z | - |
| dc.date.available | 2008-07-28 | |
| dc.date.copyright | 2005-07-28 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-26 | |
| dc.identifier.citation | 林青丘。2004。魚用生物包埋型口服疫苗製備與有效性測試。國立成功大學生物科技研究所碩士論文。
張鴻猷、王建雄、蔡貴雄。2001。水產動物疾病細菌篇、黴菌篇，雲林縣家畜防治所21-28頁，40-50頁。 廖睿雯。2004。自不同魚種分離之神經壞死症病毒株病源性的比較。國立台灣大學動物學研究所碩士論文。 鄭存明。2001。魚類結病毒持續性感染之研究。國立台灣大學動物學研究所碩士論文。 謝淨如。2002。魚類神經壞死症病毒單源抗體之建立、定性及應用。國立台灣大學動物學研究所碩士論文。 顧倩君。2002。台灣石斑虹彩病毒防治對策之相關研究。國立台灣海洋大學水產養殖研究所碩士論文。 Alonso, A., de Mello, P.A., Gormes, I. Rosenberg, F.（1975）The use of virus infection associated antigen（VIA）in the detection of cattle exposed to foot and mouth disease. Bull. C.P.F.A., 17-24 Amend D.F., Fender D.C.（1976）Uptake of bovine serum albumin by rainbow trout from hyperosmotic infiltration: a model for vaccinating fish . Science 192, 793-794 Arimoto, M., Mushiake, K., Mizuta, Y.（1992）Detection of striped jack nervous necrosis virus（SJNNV）by enzyme-linked immunosorbent assay（ELISA）. Fish Pathology 27,191-195 Arimoto, M., Mori, K., Nakai, T., Muroga, K. ＆ Furusuwa, I.（1993）Pathogenecity of the causative agent of viral nervous necrosis disease of striped jack, Pseudocarnax dentex（Bloch＆Schneider）. Journal of Fish Diseases 16, 461-469 Arimoto, M., Sato, J., Maruyama, K., Mimura, G. ＆ Furusuwa, I.（1996）Effect of chemical and physical treatment on the inactivation of striped jack nervous necrosis virus（SJNNV）. Aquaculture 143, 15-22 Awad M.A., Nusbaum K.E., Brady Y.J.（1989）Preliminary studies of a newly developed subunit vaccine for channel catfish virus disease. Journal of aquatic animal health 1, 233-237 Azad I.S., Shekhar M.S., Thirunavukkarasu A.R., Poornima M., Kailasam M., Rajan J.J.S., Ali S.A., Abraham M., Ravichandran P.（2005）Nodavirus infection causes mortalities in hatchery produced larvae of Lates calcarifer：first report from India. Diseases of Aquatic Organisms 63,113-118 Beck, E. and Strohmaier, K.（1987）. Subtyping of European foot and mouth disease strains by nucleotide sequence determination. Journal of virology. 61, 1621-1629 Bloch, B., Gravningen, K., Larsen, J.L.（1991）Encephalomyelitis among turbot associated with a picornavirus-like agent. Diseases of Aquatic Organisms 10, 65-70 Bovo, G., Maltese, C., Mutinelli, F., Rosin, R. ＆ Montesi, F.（1999a）A rapid and efficient method for diagnosing viral encephalopathy in symptomatic specimens. Abstract P-162. In: Ninth Intenational Conference of European Association of Fish Pathologistis, 19-24 September, Rhodes. Bovo, G., Nishizawa, T., Maltese, C., Borghesan, F., Mutinelli, F., Montesi, F., De Mas, S.（1999b）Viral encephalopathy and retinopathy of farmed marine fish species in Italy. Virus Research. 63, 143-146 Breuil, G., Bonami, J.R., Pepin, J.F., Pichot, Y.（1991）Viral infection（picona-like virus）asscociated with mass mortalities in hatchery-reared sea-bass（Dicentrarchus labrax）larvae and juveniles. Aquaculture 97, 109-116 Breuil, G., Pepin, J.F., Castric,J., Fauvel, C., Thiery, R.（2000） Detection of serum antibodies against nodavirus in wild and farmed adult sea bass : application to the screening of broodstock in sea bass hatcheries. Bulletin of the European association of fish pathologists. 20, 95-100 Brown, F. and Crick, J.（1959）.Application of agar-gel diffusion analysis to a study of the antigenic structure of inactivated vaccines prepared from the virus of foot and mouth disease. Journal of immunology 82, 444-447 Chang, S.F., Ngoh, G.H., Kueh, L.F.S., Qin, Q.W., Chen ,C.L., Lam, T.J. Sin, Y.M.（2001）Development of a tropical marine fish cell line from Asian seabass（Lates calcarifer）for virus isolation. Aquaculture 192, 133-145 Chi, S.C., C.F. Lo, G.H. Kou, P.S. Chang, S.E. Peng, S.N. Chen. (1997) Mass mortalities associated with viral nervous necrosis(VNN) disease in two species of hatchery-reared grouper, Epinephelus fuscogutatus and Epinephelus akaara (Temminck & Schlegel). Journal of Fish Diseases 20, 185-193. Chi, S.C., Hu, W.W., Lo, B.J.（1999）Establishment and characterization of a continuous cell line（GF-1）derived from grouper, Epinephelus coioides（Hamilton）：a cell line susceptible to grouper nervous necrosis virus（GNNV）. Journal of Fish Diseases 22, 1-10. Chi, S.C., Lo, B.J., Lin, S.C., Wen, W.W., Lo, C.F., Kou, G.H., Chen, S.N.（2000）The survey of viral nervous necrosis among cultured groupers in Taiwan. In: APEC FWG02/2000. Bangkok, 18-20 Chi, S.C., Lo, B.J., Lin, S.C.（2001）Characterization of grouper nervous necrosis virus（GNNV）. Journal of Fish Diseases 24, 3-13 Chi, S.C., Shieh, J. R., Lin S.J.（2003）Genetic and antigenic analysis of betanodaviruses isolated frome aquatic organisms in Taiwan. Diseases of Aquatic Organisms 55, 221-228 Comps, M., Trindade, M., Delsert, C.L.（1996）Investigation of fish encephalitis viruses（FEV）expression in marine fishes using DIG-labelled probes. Aquaculture 143, 113-121 Corbeil, S.; LaPatra, S.E.; Anderson, E.D.; Kurath, G. （2000）Nanogram quantities of a DNA vaccine protect rainbow trout fry against heterologous strains of infectious hematopoietic necrosis virus. Vaccine 18, 2817-2824 Dalla Valle, L., Zanella, L., Patarnello, P., Paolucci, L., Belvedere, P. Colombo, L.（2000）Development of a sensitive diagnostic assay for fish nervous necrosis virus based on RT-PCR plus nested PCR. Journal of Fish Diseases 23, 321-327 Dasgupta, R., Ghosh, A., Dasmahapatra, B., Guarino, L.A., Kaesberg, P.（1984）Primary and secondary structure of black beetle virus RNA2, the genomic messenger for BBV coat protein. Nucleic Acid Res.12, 7215-7223 Dasgupta, R. and Sgro, J.Y.（1989）Nucleotide sequences of three nodavirus RNA2’s : the messengers for their coat protein precursors. Nucleic Acid Res.17, 7525-7526 Dixon P.F., Hill B.J.（1983）Rapid detection of infectious pancreatic necrosis virus (IPNV) by the enzyme-linked immunosorbent assay (ELISA). Journal of General Virology 64, 321-330. Fellowes, O.N.（1965）Comparison of the inactivation and antigenicity of foot and mouth disease virus by acetylethylenimine and by combined effect of ultraviolet light and β-propiolactone. Journal of immunology. 95: 1100-1106 Frerichs, G.N., Morgan, D., Hart, D., Skerrow, C., Roberts, R.J., Onions, D.E.（1991）Spontaneously productive C-type retrovirus infection of fish cell lines. Journal of General Virology 72, 2537-2539 Frerichs, G.N., Rodger, H.D., Peric, Z.（1996）Cell culture isolation of piscine neuropathy nodavirus from juvenile sea bass , Dicentrchus labrax. Journal of Virology 77, 2067-2071 Frerichs, G.N.; Tweedie, A.; Starkey, W.G.; Richards, R.H.（2000） Temperature, pH and electrolyte sensitivity, and heat, UV and disinfectant inactivation of sea bass (Dicentrarchus labrax) neuropathy nodavirus. Aquaculture 185, 13-24 Frost, P. and Ness, A.（1997）Vaccination of Atlantic salmon with recombinant VP2 of infectious pancreatic necrosis virus (IPNV), added to a multivalent vaccine, suppresses viral replication following IPNV challenge. Fish & Shellfish Immunology 7, 609-619 Fukuda, Y., Neuyen, H.D., Furuhashi, M., Nakai, T.（1996）Mass mortality of cultured sevenband grouper, Epinephelus septemfasciatus, associated with viral nervous necrosis. Fish Pathology 31, 165-170 Glazebrook, J.S., Heaaman, M.P., de Beer, S.W.（1990）Picona-like viral particles associated with mass mortalities in larval barramundi, lates calcarifer Bloch. Journal of Fish Diseases 13, 245-249 Grotmol, S., Totland, G.K., Kvellestad, A., Fjell, K., Olsen, A.B.（1995）Mass mortality of larval and juveniles hatchery-reared halibut（Hippoglossus hippoglossus L.）associated with the presence of virus-like particles in vacuolated lesions in the central nervous system and retina. Bull Eur Ass Fish Pathol 15（5）,176-180 Grotmol, S., Totland, G.K., Kryvi, H.（1997）Detection of a nodavirus-like agent in heart tissue from reared Atlantic salmon Salmo salar suffering from cardiac myopathy syndrome（CMS）. Diseases of Aquatic Organisms 29, 79-84 Grotmol, S., Bergh, O., Totland, G.K.（1999）Transmission of viral encephalopathy and retinopathy（VER）to yolk-sac larvae of the Atlantic halibut Hippoglossus hippoglossus：occurrence of nodavirus in various organs and a possible route of infection. Diseases of Aquatic Organisms 39, 95-106 Grotmol,S. and Totland,G.K.（2000）Surface disinfection of Atlantic halibut Hippoglossus hippoglossus eggs with ozonated sea-water inactivates nodavirus and increases survival of the larvae. Diseases of Aquatic Organisms 39, 89-96 Heppell, J., Lorenzen, N., Armstrong, N.K.., Wu, Tong, Lorenzen, E., Einer-Jensen, K., et. al. （1998）Development of DNA vaccines for fish: vector design, intramuscular injection and antigen expression using viral haemorrhagic septicaemia virus genes as model. Fish & Shellfish Immunology 8, 271-286 Húsgarõ, S., Grotmol, S., Hjeltnes, B.K., Rodseth, O.M., Biering, E.（2001）Immune response to a recombinant capsid protein of striped jack nervous necrosis virus（SJNNV）in turbot Scophthalmus maximus and Atlantic hulibut Hippoglossus hippoglossus, and evalution of a vaccine against SJNNV. Diseases of Aquatic Organisms 45, 33-44 Iwamoto, T., Mori, K., Arimoto, M., Nakai, T.（1999）High permissivity of the fish cell line SSN-1 for piscine nodaviruses. Diseases of Aquatic Organisms 39, 37-47 Iwamoto, T., Nakai, T., Mori, K., Arimoto, M., Furusawa, I.（2000）Cloning of the fish cell line SSN-1 for piscine nodaviruses. Diseases of Aquatic Organisms 43, 81-89 Iwamoto, T., Mori, K., Arimoto, M., Nakai, T.（2001）A combined cell-culture and RT-PCR method for rapid detection of piscine nodaviruses. Journal of Fish Diseases 24, 231-236 Jarp, J., Tverdal, A.（1997）Statitical aspects of fish vaccination trials. Fish vaccinology. Developments in Biological standardization. 90, 311-320 Johnson KA., Flynn JK., Amend DF.（1982）Onset of immunity in salmonid fry vaccinated by direct immersion in Vibrio anguillarum and Yersinia ruckeri bacterins. Journal of Fish Diseases 5,197-205 Jósefsson, Snorri and Tatner, Mary F.（1993） Histogenesis of the lymphoid organs in sea bream (Sparus aurata L.). Fish & Shellfish Immunology 3, 35-49 Jung, S.J., Miyazaki, T., Miyata, M., Oishi, T.（1996）Histopathological studies on viral nervous necrosis in a new host Japanese sea bass Lateolabrax japonicus. Bulletin of the Faculty of Bioresources, Mie University 16, 9-16 Kaesberg, P. ＆ Johnson, J.E.（1990）Structural homology among four nodaviruses as dedused by sequencing and X-ray crystallography. J. Mol. Biol. 214, 423-435 Lai, Y.S., Chiu, H.C., Murali, S., Guo, I.C., Chen, S.C., Fang, K., Chang, C.Y.（2001）In vitro neutralization by monoclonal antibodies against yellow grouper nervous necrosis virus （YGNNV）and immunolocalization of virus infection in yellow grouper, Epinephelus awoara（Temminck＆Schlegel）. Journal of Fish Diseases 24, 237-244 Le Breton, A., Grisez, L., Sweetman, J., Ollevier, F.（1997）Viral nervous necrosis（VNN）associated with mass mortalities in cage-reared sea bass, Dicentrarchus labrax（L.）. Journal of Fish Diseases 20, 145-151 Lillehaug A., Ramstad A., Baekken K., Reitan LJ.（1993）. Protective immunity in Atlantic salmon（Salmo salar L.）vaccinated at different water temperatures. Fish and shellfish immunology 3, 143-156 Lin, H.Y., Kuo, T.Y., Huang, H.I., Yu, C.J., Liang, H.C., Chi, S.C., and Yang, H.L.（2001）Immuno-response to killed vaccine and recombinant sub-unit vaccine of nervous necrosis virus in grouper. Abstract P-185. In: Tenth Intenational Conference of European Association of Fish Pathologistis, 9-14 September, Dublin Magarino B., Romalde JL., Santos Y., Casal JF., Barja JL., Toranzo AE（1994）. Vaccination trials on gilthead seabream（Sparus aurata）against Pasteurella piscicida. Aquaculture, 120:201-208 Mano, N., T. Inui, D. Arai, H. Hirose and Y. Deguchi（1996）. Immune response in the skin of eel against Cytophaga columnaris. Fish pathology, 31, 65-70 Moore, J., Ototake, M., Nakanishi, T.（1998）Particulate antigen uptake during immersion immunization of fish: The effectiveness of prolonged exposure and the roles of skin and gill. Fish and Shellfish Immunology 8, 393-407 Mori, K., Nakai, T., Negahara, M., Muroga, K., Mckuchi, T. ＆ Kanno, T.（1991）A viral disease in hatchery-reared larvae and juveniles of redspotted grouper. Fish Pathology 26, 209-210 Mori, K., Nakai, T., Muroga, K., Arimoto, M., Mushiake, K., Furusawa, I.（1992）Properties of a new virus belonging to nodaviridae found in larval striped jack（Pseudocaranx dentex）with nervous necrosis. Virology 187, 368-371 Munday, B.L., Langdon, J.S., Hyatt, A., Humphrey, J.D.（1992）Mass mortality associated with a viral-induced vacuolating encephalopathy and retinopathy of larval and juvenile barramundi, Lates calcarfer Bloch. Aquaculture 103, 197-211 Munday, B.L. ＆ Nakai, T.（1997）Special topic review : nodaviruses as pathogens in larval and juvenile marine finfish. World J. Micro. and Biotech. 13, 375-381 Munday, B.L., Kwang, J., Moody, N.（2002）Betanodavirus infection of teleost fish : a review. Journal of Fish Diseases 25, 127-142 Muroga, K.（1995）Viral and bacterial disease in larval and juvenile marine fish and shellfish: a review. Fish Pathology 30, 71-85 Mushiake, K., Arimoto, M., Furusawa, T., Furusawa, I., Nakai, T. ＆Muroga, K.（1992）Detection of antibodies against striped jack nervous necrosis virus（SJNNV）from brood stocks of striped jack. Nippon Suisan Gakkaishi 58, 2351-2356 Mushiake, K., Nishizawa, T., Nakai, T., Furusawa, I., ＆ Muroga, K.（1994）Control of VNN in striped jack : selection of spawners based n the detection of SJNNV gene by polymerase chain reaction（PCR）. Fish Pathology 29,177-182 Nakai, T., Nguyen, H.D., Nishizawa, T., Muroga, K., Arimoto, M., Ootsuki, K.（1994）Occurrence of viral nervous necrosis in kelp grouper and tiger puffer. Fish Pathology 29, 211-214 Nakai, T., Mori, K., Nishizawa, T. and Muroga, K.（1995）Viral nervous necrosis of larval and juvenile marine fish. Asian Fisheries Society Special Publication 10,147-152 Nakanishi, T. and Ototake, M.（1997）Antigen uptake and immune responses after immersion vaccination. Fish Vaccinology. Dev Biol stand 90,59-68 Nakanishi T., Kiryu I., Ototake M.（2002）Development of a new vaccine delivery method for fish: percutaneous administration by immersion with application of a multiple puncture instrument. Vaccine 20, 3764-3769 Nam,B.H., Hirono,I. and Aoki,T. （2003）The four TCR genes of teleost fish:the cDNA and genomic DNA analysis of Japanese flounder （paralichthys olivaceus）TCR alpha-,beta-,gamma-,and delta-chains. Journal of Immunology.170, 3081-3090 Nguyen, H.D., Mekuchi, T., Imura, K., Nakai, T., Nishizawa, T., Muroga, K.（1994）Occurrence of viral nervous necrosis（VNN）in hatchery-reared juvenile Japanese flounder Paralichthys olivaceus. Fisheries Science. 60, 551-554 Nguyen, H.D., Nakai, T., Muroga, K.（1996）Progression of striped jack nervous necrosis virus（SJNNV）in adult striped jack Pseudocaranx dentex larvae. Diseases of Aquatic Organisms 24, 99-105 Nishizawa, T., Mori, K., Nakai, T.（1994）Polymerase chain reaction（PCR）amplification of RNA of striped jack nervous necrosis virus（SJNNV）. Diseases of Aquatic Organisms 18, 103-107 Nishizawa, T., Masaaki, K., Nakai, T. and Muroga, K.（1995）Comparison of the coat protein genes of five fish nodaviruses, the causative agent of nervous necrosis in marine fish. Journal of General Virology 76, 1563-1569 Nishizawa, T., Furuhashi, M., Nagal, T. ＆ Muroga, K.（1997）Genomic classification of fish nodaviruses by molecular phylogenetic analysis of the coat protein gene. Appl. and Envir. Micro. 63, 1633-1636 Ototake, M., Ikeda, K., Nakanishi, T.（1992）Comparison of uptake of bovine serum albumin by marine and freshwater fish after hyperosmotic infiltration treatment. Aquaculture 103,187-194 Ototake, M., Iwama, G., Nakanishi, T.（1996）The uptake of bovine serum albumin by the of bath-immunised rainbow trout Oncorhynchus mykiss. Fish and Sellfish Immunology 6, 321-333 Perelberg, A., Ronen, A., Hutoran, M., Smith, Y., Kotler, M.（2005）. Protection of cultured Cyprinus carpio against a lethal viral disease by an attenuated virus vaccine. Vaccine 23, 3396-3403 Renault, T., Haffner, P.H., Baudin, L.F., Breuil, G., Bonami, J.R.（1991）Mass mortalities in hatchery-reared sea bass（Lates calcarifer）larvae associated with the presence in the brain and retina of virus-like particles. Bull Eur Ass Fish Pathol.11, 68-73 Soria Moria Hotel, O.（1996）Fish vaccinology. 50-65 Skliris, G.P. and Richards, R.H.（1998）. Assessment of the susceptibility of the brine shrimp Artemia salina and rotifer Brachionus plicatilis to experimental nodavirus infection. Aquaculture 169, 133-141 Snorri Josefsson and Tatner M.F. （1993）Histogenesis of the lymphoid organs in sea bream. Fish and shellfish immunol 3, 35-49 Sommerset, I., Husgaard, S., Nerland, A.H.（2001） Development of vaccines against nodavirus affecting Atlantic halibut（Hippoglossus hippoglossus）. Abstract P-113. In: Tenth Intenational Conference of European Association of Fish Pathologistis, 9-14 September, Dublin Tanaka, S., Mori, K., Arimoto, M., Iwamoto, T., Nakai, T.（2001）Protective immunity of sevenband grouper Epinephelus septemfasciatus Thunberg, against experimental viral nevous necrosis. Journal of Fish Diseases 24, 15-22 Tatner M.F. and Horne M.T.（1983）Factors influencing the uptake of 14C-labelled Vibrio anguillarum vaccine in direct immersion experiments with rainbow trout, Salmo gairdneri Richardson. J Fish Biol 22, 585-591. Tatner MF. and Horne MT.（1985）The effect of vaccine dilution, length of immersion time, and booster vaccinations on the protection levels induced by direct immersion vaccination of brown trout, Salmo trutta , with Yersinia ruckeri（ERM）vaccine. Aquaculture 46, 11-18 Tatner MF.（1987）The quantitative relationship between vaccine dilution length of immersion time and antigen uptake using a radiolabelled Aeromonas salmonicida bath in direct immersion experiments with rainbow trout, Salmo gairdneri. Aquaculture 62, 173-186 Watanabe, K., Nishizawa, T., Yoshimizu, M.（2000）Selection of brood stock candidates of barfin flounder using an ELISA system with recombinant protein of barfin flounder nervous necrosis virus. Diseases of Aquatic Organisms 41, 219-223 Yoshikoshi, K. and Inoue, K.（1990）Viral nervous necrosis in hatchery- reared larvae and juveniles of Japanese parrotfish, Oplegnathus fasciatus（Temminck＆Schlegel）. Journal of Fish Diseases 13, 69-77 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35867 | - |
| dc.description.abstract | 在台灣，病毒性神經壞死症（Viral nervous necrosis disease, VNN disease）每年均造成養殖業重大經濟損失，神經壞死症病毒主要感染石斑魚苗及幼魚，並引起高達80%~100%的死亡率。本研究主要為發展NNV去活化疫苗，並以浸泡法免疫魚苗，達到對抗NNV感染之目的。將NNV感染GF-1細胞，收集出現完整CPE後的病毒液，用於製備去活化疫苗及攻毒實驗。以相對存活率（Relative Percentage Survival, RPS）來評估疫苗的保護效果，免疫魚隻攻毒後RPS值至少要大於30%才具有顯著效果。本實驗先以四組不同的去活化疫苗（F-I,F-II,BPL,BEI）免疫石斑魚苗，結果顯示F-I,F-II和BEI組皆有顯著的保護效果，但BPL卻無。四組疫苗中，以BEI所製備的疫苗保護效果最好，RPS值高達83%~95%。其次，比較奈米包埋後的疫苗對免疫效果的影響，將F-II疫苗經奈米包埋後，以105 TICD50/ml病毒劑量免疫魚苗，攻毒後RPS值高達83%，保護效果最好，其RPS值甚至高於100倍病毒劑量的F-II未包埋疫苗組。比較不同浸泡濃度及浸泡時間對疫苗保護效果的影響，結果BEI疫苗的最低有效劑量為106 TICD50/ml。浸泡免疫20分鐘即可引發良好的保護效果。由浸泡攻毒實驗結果顯示，浸泡免疫一次BEI疫苗，保護效果至少可維持3個月。因此，使用NNV去活化疫苗浸泡免疫石斑八分苗，將成為重要且有效的防疫方法。 | zh_TW |
| dc.description.abstract | Nervous necrosis virus (NNV) is the most economically important pathogen of cultured groupers in Taiwan causing mass mortality 80~100% in fry and larvae. The aim of this study is to develop inactivated NNV vaccine, and a method of treating or preventing VNN through immersion immunization of grouper fry. The culture supernatant of NNV-infected GF-1 cells was collected after cytopathic effect (CPE) complete, and used for the preparation of inactivated vaccine and for challenge test. The effectiveness of vaccine was expressed by relative percentage survival (RPS), and significant protection usually means a RPS for vaccinated fish of at least above 30%. Four sorts of inactivated NNV vaccines (F-I, F-II, BPL, BEI) were prepared and used for immersion immunization of grouper fry. The result revealed that the inactivated vaccines (F-I, F-Il, BEI) could protect grouper fry from NNV infection, but BPL-prepared NNV vaccine failed to provide protection. Particularly, the RPS of BEI group was very high (83%~95%), and no virus was detected in vaccinated fish. Subsequently, the comparative results of vaccine without and with nano-encapsulation showed that effectiveness of nano-encapsulated vaccine improved. The RPS of fish immunized by encapsulated F-II vaccine was 83%, much higher than that of un-capsulated F-II vaccine with 100-fold viral concentration. The minimal NNV concentration for effective protection of BEI un-capsulated vaccine was 106 TICD50/ml. In addition, it was found that 20 min was enough for immersion immunization, but one month was necessary for the development of efficacious immunity after vaccination. Finally, the result of immersion challenge indicated that the protection of the vaccination could last for at least three months (RPS=82%). Consequently, NNV inactivated vaccine is believed to become an important approach for the control of NNV disease. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T07:47:41Z (GMT). No. of bitstreams: 1 ntu-94-R92b41024-1.pdf: 540909 bytes, checksum: e82b16484495b1a2907707dab53b1dda (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 目錄 Ⅰ
圖表目次 Ⅳ 摘要 Ⅴ Abstract Ⅵ 前言… Ⅶ 第一章 文獻回顧 1.神經壞死症病毒 1.1神經壞死症病毒之病史及病癥 1 1.2神經壞死症病毒特性及分類地位 1 1.3神經壞死症病毒的物、化特性 2 1.4神經壞死症病毒地理分佈與宿主 3 1.5神經壞死症病毒流行病學 3 1.6神經壞死症病毒散佈之病程及在宿主體內之分佈 5 1.7神經壞死症病毒診斷方法 6 1.8神經壞死症病毒之防疫 8 2. 魚類病毒性疾病疫苗的發展 2.1減毒疫苗 10 2.2去活化疫苗 10 2.3 DNA疫苗 11 2.4次單位疫苗 11 3.免疫方式 3.1注射式免疫 12 3.2口服式免疫 12 3.3浸泡式疫苗 13 第二章 材料與方法 1. NNV去活化條件測試 1.1細胞株 16 1.2病毒 16 1.3病毒力價測定 16 1.4去活化疫苗的製備及安全性評估 16 1.5間接酵素連結免疫吸附法 17 2. 去活化疫苗對魚苗的保護效果評估 2.1實驗魚隻 18 2.2病毒 18 2.3疫苗 18 2.4浸泡式免疫 18 2.5活體攻毒測試 19 2.6 RNA之萃取.. 19 2.7反轉錄-聚合酶連鎖反應 19 2.8 Semi - nested PCR 20 2.9 疫苗保護效力的評估 20 第三章 結果 1.NNV去活化條件測試 1.1 Formalin 22 1.2 BPL 22 1.3 BEI 22 2.去活化藥劑的處理對病毒抗原保留性之初步評估 2.1 Formalin 23 2.2 BPL 23 3.NNV去活化疫苗對石斑魚苗保護效果評估 3.1不同藥品製備之疫苗對石斑魚苗保護效果評估 23 3.2奈米包埋後疫苗對石斑魚苗保護效果評估 …23 3.3浸泡條件測試 24 3.3.1浸泡濃度 24 3.3.2浸泡時間 24 3.4有效保護期限測試 24 第四章 討論 1.各藥品製成疫苗的比較 25 2.奈米包埋後NNV去活化疫苗對石斑魚苗的保護效果比較 28 3.疫苗使用的適當濃度及時間 29 4.有效保護期限測試 30 5.疫苗保護的機制 31 6.展望 32 第五章 參考文獻 34 | |
| dc.language.iso | zh-TW | |
| dc.title | 石斑魚神經壞死症病毒去活化疫苗的製備及效能評估 | zh_TW |
| dc.title | Development and evaluation of the inactivated vaccine against grouper nervous necrosis virus | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 周信佑,羅竹芳,宋延齡 | |
| dc.subject.keyword | 神經壞死症病毒,疫苗,石斑魚, | zh_TW |
| dc.subject.keyword | NNV,vaccine,grouper, | en |
| dc.relation.page | 56 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-07-26 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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