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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35664完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 嚴震東(Chen-Tung Yen) | |
| dc.contributor.author | Chao-Yi Ho | en |
| dc.contributor.author | 何昭儀 | zh_TW |
| dc.date.accessioned | 2021-06-13T07:03:46Z | - |
| dc.date.available | 2005-07-30 | |
| dc.date.copyright | 2005-07-30 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-27 | |
| dc.identifier.citation | Angel A, Clarke KA. An analysis of the representation of the forelimb in the ventrobasal thalamic complex of the albino rat. J Physiol. 1975 Jul;249(2):399-423
Barbaresi P, Spreafisco R, Frassoni C, Rustioni A. GABAergic neurons are present in the dorsal column nuclei but not in the ventroposterior complex of rats. Brain Res. 1986 Sep 24;382(2):305-26. Chung JM, Lee KH, Surmeier DJ, Sorkin LS, Kim J, Willis WD. Response characteristics of neurons in the ventral posterior lateral nucleus of the monkey thalamus. J Neurophysiol. 1986 Aug;56(2):370-90. Chiaia NL, Rhoades RW, Fish SE, Killackey HP. Thalamic processing of vibrissal information in the rat: II. Morphological and functional properties of medial ventral posterior nucleus and posterior nucleus neurons. J Comp Neurol. 1991 Dec 8;314(2):217-36. De Biasi S., Frassoni C. and Spreafisco R. GABA immunoreactivity in the thalamic reticular nucleus of the rat. A light and electron microscopical study. Brain Res. 1986 Dec 3;399(1):143-7. Fuentealba P, Steriade M. The reticular nuecleus revisited: Intrinsic and network properties of a thalamic pacemaker. Progress in Neurobiology 2005 (75): 125-141. Guilbaud G, Peschanski M, Gautron M, Binder D. Neurones responding to noxious stimulation in VB complex and caudal adjacent regions in the thalamus of the rat. Pain. 1980 Jun;8(3):303-18. Hartings JA, Temereanca S, Simons DJ. High responsiveness and direction sensitivity of neurons in the rat thalamic reticular nucleus to vibrissa deflections. J Neurophysiol. 2000 May;83(5):2791-801. Jones EG. The Thalamus. Plenum Press, New York. 1985. Kandel ER, Schwartz JH, Jessell TM. Principles of Neural Science, 4th edition. McGraw-Hill Companies.2000. Lubke J. Morphology of neurons in the thalamic reticular nucleus (TRN) of mammals as revealed by intracellular injections into fixed brain slices. J Comp Neurol. 1993 Mar 22;329(4):458-71. Kenshalo DR Jr, Giesler GJ Jr, Leonard RB, Willis WD. Responses of neurons in primate ventral posterior lateral nucleus to noxious stimuli. J Neurophysiol. 1980 Jun;43(6):1594-614. Montagne-Clavel J, Oliveras JL. Does barbiturate anesthesia modify the neuronal properties of the somatosensory thalamus? A single-unit study related to nociception in the awake-pentobarbital-treated rat. Neurosci Lett. 1995 Aug 18;196(1-2):69-72. Peschanski M, Guilbaud G, Gautron M, Besson JM. Encoding of noxious heat messages in neurons of the ventrobasal thalamic complex of the rat. Brain Res. 197, 401-414. Peschanski M, Guilbaud G, Gautron M. Neuronal responses to cutaneous electrical and noxious mechanical stimuli in the nucleus reticularis thalami of the rat. Neurosci Lett. 1980 Nov;20(2):165-70. Pinault D. The thalamic reticular nucleus: structure, function and concept. Brain Res Brain Res Rev. 2004 Aug;46(1):1-31. Review. Spreafico R, De Curtis M, Frassoni C, Avanizini G. Electrophysiological characteristics of morphologically identified reticular thalamic neurons from rat slices. Neuroscience. 1988 Nov;27(2):629-38. Steriade M, Jones EG, Llina RR. Thalamic oscillations and signaling. Wiley- Interscience, New York. 1990. Steriade M, Jones EG, McCormick DA. Thalamus, Vol.1. Elsevier Science. 1997. Steriade M, Jones EG, McCormick DA. Thalamus, Vol.2. Elsevier Science. 1997. Thierry B, McCormick DA. Mechanisms of oscillatory activity in guinea-pig nucleus reticularis thalami in vitro: a mammalian pacemaker. J Physio. 1993 ;468:669-691. Yen CT, Shaw FZ. Reticular thalamic responses to nociceptive inputs in anesthetized rats. Brain Res. 2003 Apr 11;968(2):179-91. Yen CT, Conley M, Jones EG. Morphological and functional types of neurons in cat ventral posterior thalamic nucleus. J Neurosci. 1985 May;5(5):1316-38. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35664 | - |
| dc.description.abstract | 大鼠腦中的腹背側核 (VPL) 和網狀核 (Rt) 是傳遞體感覺的重要中繼站，來自週遭的體感覺訊息在此會合, 然後上傳至大腦皮質，產生感覺。這個實驗在研究並比較大鼠VPL和Rt中神經元的生理及形態特性，並進一步依照神經元對自然刺激和電刺激的反應, 來訂出在這兩個核區中的亞群。在麻醉的大鼠VPL和Rt內，我們紀錄了細胞外單一神經元的自發性電生理訊號，並分析神經元在週遭受到自然刺激或電刺激時的活性改變。進一步地，我們在腦片中，用whole-cell patch-clamp recording的方式，紀錄Rt和VPL的電生理特性，同時在實驗結束後，染色分析Rt和VPL細胞核的形態。
實驗結果顯示，VPL和Rt神經元有著顯著不同，例如: 波形、對不同刺激的反應、和形態。但是在對刺激有著不同反映的次亞群中，我們無法找出他們在波形上的差異。 | zh_TW |
| dc.description.abstract | Thalamic reticular nucleus (Rt) and ventroposter somatosensory signals en route to the cerebral cortex. This study sought to compare and characterize ior lateral nucleus (VPL) are two important thalamic nuclei processing electrical and morphological properties of Rt and VPL, and to classify functional subclasses in the two regions by their electrical properties. Extracellular single-unit activities of Rt and VPL were recorded in anesthetized rats. Spontaneous firing pattern and responsive pattern to tactile or noxious stimuli were used to subdivide the units into functional subclasses. In addition, Rt and VPL neurons were recorded in slice preparations by whole-cell patch technique. Morphological differences between Rt and VPL neurons were analyzed.
Our results showed that VPL and Rt neurons differed significantly in their electrical activities, such as spike waveforms, response patterns to different stimuli, and cell morphology. However, subclasses in the two regions differed in their response patterns but not their waveforms. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T07:03:46Z (GMT). No. of bitstreams: 1 ntu-94-R92b41008-1.pdf: 582448 bytes, checksum: b8192279ac109902646e57fa91ea54f0 (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 1. 中文摘要…………………………………………………………………………1
2. Abstract…………………………………………………………………………..2 3. Introduction………………………………………………………………………3 3.1. Somatosensory system………………………………………………………..3 3.2. Modulation of somatosensory information at the thalamic level………….4 3.3. Morphological and physiological properties of VPL……………………...4 3.4. Morphological and physiological properties of Rt………………………...6 3.5. Goals of the study……………………….…..……………………………….7 4. Materials and Methods………………………………………………………...8 4.1. In vivo single unit recording………………………………………………..8 4.1.1. Animal preparations ………………………………………………......8 4.1.2. Extracellular single-unit recording………………………………….8 4.1.3. Data collection and analysis………………………………………...9 4.1.4 Histology……………………………………………………………...10 4.2. In vitro whole-cell patch-clamp recording…………………………………..10 4.2.1. Slice preparation…………………………………………………...10 4.2.2. Whole-cell patch-clamp recording…………………………………...10 4.2.3. Histology……………………………………………………………..11 5. Result………………………………………………………………………… 13 5.1. Classification of Rt and VPL neurons…………………………………….13 5.2. Quantification and comparison of Rt and VPL waveform parameters……...15 5.3. Responses of Rt and VPL to natural simulation…………………………….16 5.4. Responses of Rt and VPL to electrical stimulation……………………….16 5.5. Correlation between functional subclasses defined by natural stimulation and by electrical stimulation…………………… ………………………….17 5.6. Correlation between functional subclasses and spike waveforms…………..18 5.7. Morphology of recorded neurons in vitro………………………………… 19 5.8. Electrical properties of recorded neurons in vitro…………………………19 6. Discussion……………………………………………………………………..20 6.1. Waveforms of Rt and VPL…………………………………………………..20 6.2. Functional Subclasses of Rt and VPL……………………………………….22 6.3. Neurons in different functional subclasses have similar waveforms………..24 6.4. Electrical properties of Rt and VPL neurons in vitro……………...……….25 7. Reference……………………………………………………………………...27 8. Tables…………………………………………………………………………...30 9. Figures…………………………………………………………………………...32 | |
| dc.language.iso | en | |
| dc.subject | 體感覺 | zh_TW |
| dc.subject | 丘腦 | zh_TW |
| dc.subject | 腹後側核 | zh_TW |
| dc.subject | 網狀核 | zh_TW |
| dc.subject | 電生理 | zh_TW |
| dc.subject | 波形 | zh_TW |
| dc.subject | Thalamic Reticular nucleus(Rt) | en |
| dc.subject | somatosensory system | en |
| dc.subject | waveform | en |
| dc.subject | extracellular single unit recording | en |
| dc.subject | Ventroposterior Lateral nucleus (VPL) | en |
| dc.title | 大鼠丘腦腹後側核及網狀核神經元的生理及形態特性比較 | zh_TW |
| dc.title | Comparison of Physiological and Morphological Characteristics of Thalamic Reticular and Ventroposterior Lateral Neurons of the Rat | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 閔明源(Ming-Yuan Min),陳瑞芬(Ruei-Feng Chen),徐百川(Bai-Chuang Shyu) | |
| dc.subject.keyword | 丘腦,腹後側核,網狀核,電生理,波形,體感覺, | zh_TW |
| dc.subject.keyword | Thalamic Reticular nucleus(Rt),Ventroposterior Lateral nucleus (VPL),extracellular single unit recording,waveform,somatosensory system, | en |
| dc.relation.page | 52 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-07-27 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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