請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35652完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 孔繁璐 | |
| dc.contributor.author | Kai-Wei Lin | en |
| dc.contributor.author | 林愷薇 | zh_TW |
| dc.date.accessioned | 2021-06-13T07:03:09Z | - |
| dc.date.available | 2015-07-27 | |
| dc.date.copyright | 2005-08-04 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-27 | |
| dc.identifier.citation | 參考文獻
1. Glenner, G.G. and Wong, C.W., Alzheimer's disease: initial report of the purification and characterization of a novel cerebrovascular amyloid protein. Biochem Biophys Res Commun, 1984. 120(3): pp. 885-90. 2. Selkoe, D.J., Normal and abnormal biology of the beta-amyloid precursor protein. Annu Rev Neurosci, 1994. 17: pp. 489-517. 3. Yankner, B.A., Mechanisms of neuronal degeneration in Alzheimer's disease. Neuron, 1996. 16(5): pp. 921-32. 4. Mattson, M.P., Cellular actions of beta-amyloid precursor protein and its soluble and fibrillogenic derivatives. Physiol Rev, 1997. 77(4): pp. 1081-132. 5. Arveson, W., The curvature of a Hilbert module over. Proc Natl Acad Sci U S A, 1999. 96(20): pp. 11096-99. 6. Golde, T.E., Eckman, C.B., and Younkin, S.G., Biochemical detection of Abeta isoforms: implications for pathogenesis, diagnosis, and treatment of Alzheimer's disease. Biochim Biophys Acta, 2000. 1502(1): pp. 172-87. 7. Goldgaber, D., Lerman, M.I., McBride, O.W., Saffiotti, U., and Gajdusek, D.C., Characterization and chromosomal localization of a cDNA encoding brain amyloid of Alzheimer's disease. Science, 1987. 235(4791): pp. 877-80. 8. Yoshikai, S., Sasaki, H., Doh-ura, K., Furuya, H., and Sakaki, Y., Genomic organization of the human amyloid beta-protein precursor gene. Gene, 1990. 87(2): pp. 257-63. 9. Caswell, M.D., Mok, S.S., Henry, A., Cappai, R., Klug, G., Beyreuther, K., Masters, C.L., and Small, D.H., The amyloid beta-protein precursor of Alzheimer's disease is degraded extracellularly by a Kunitz protease inhibitor domain-sensitive trypsin-like serine protease in cultures of chick sympathetic neurons. Eur J Biochem, 1999. 266(2): pp. 509-16. 10. Ladror, U.S., Kohnken, R.E., Wang, G.T., Manelli, A.M., Frail, D.E., Klein, W.L., Holzman, T.F., and Krafft, G.A., Evidence against a role for the Kunitz domain in amyloidogenic and secretory processing of the amyloid precursor protein. J Neurochem, 1994. 63(6): pp. 2225-30. 11. Pahlsson, P., Shakin-Eshleman, S.H., and Spitalnik, S.L., N-linked glycosylation of beta-amyloid precursor protein. Biochem Biophys Res Commun, 1992. 189(3): pp. 1667-73. 12. McFarlane, I., Georgopoulou, N., Coughlan, C.M., Gillian, A.M., and Breen, K.C., The role of the protein glycosylation state in the control of cellular transport of the amyloid beta precursor protein. Neuroscience, 1999. 90(1): pp. 15-25. 13. Mills, J. and Reiner, P.B., Regulation of amyloid precursor protein cleavage. J Neurochem, 1999. 72(2): pp. 443-60. 14. Passer, B.J., Pellegrini, L., Vito, P., Ganjei, J.K., and D'Adamio, L., Interaction of Alzheimer's presenilin-1 and presenilin-2 with Bcl-X(L). A potential role in modulating the threshold of cell death. J Biol Chem, 1999. 274(34): pp. 24007-13. 15. Wilquet, V. and De Strooper, B., Amyloid-beta precursor protein processing in neurodegeneration. Curr Opin Neurobiol, 2004. 14(5): pp. 582-8. 16. Masters, C.L., Simms, G., Weinman, N.A., Multhaup, G., McDonald, B.L., and Beyreuther, K., Amyloid plaque core protein in Alzheimer disease and Down syndrome. Proc Natl Acad Sci U S A, 1985. 82(12): pp. 4245-9. 17. Lin, X., Koelsch, G., Wu, S., Downs, D., Dashti, A., and Tang, J., Human aspartic protease memapsin 2 cleaves the beta-secretase site of beta-amyloid precursor protein. Proc Natl Acad Sci U S A, 2000. 97(4): pp. 1456-60. 18. Citron, M., Oltersdorf, T., Haass, C., McConlogue, L., Hung, A.Y., Seubert, P., Vigo-Pelfrey, C., Lieberburg, I., and Selkoe, D.J., Mutation of the beta-amyloid precursor protein in familial Alzheimer's disease increases beta-protein production. Nature, 1992. 360(6405): pp. 672-4. 19. Cai, H., Wang, Y., McCarthy, D., Wen, H., Borchelt, D.R., Price, D.L., and Wong, P.C., BACE1 is the major beta-secretase for generation of Abeta peptides by neurons. Nat Neurosci, 2001. 4(3): pp. 233-4. 20. Richardson, J.C., Kendal, C.E., Anderson, R., Priest, F., Gower, E., Soden, P., Gray, R., Topps, S., Howlett, D.R., Lavender, D., Clarke, N.J., Barnes, J.C., Haworth, R., Stewart, M.G., and Rupniak, H.T., Ultrastructural and behavioural changes precede amyloid deposition in a transgenic model of Alzheimer's disease. Neuroscience, 2003. 122(1): pp. 213-28. 21. Vassar, R., Bennett, B.D., Babu-Khan, S., Kahn, S., Mendiaz, E.A., Denis, P., Teplow, D.B., Ross, S., Amarante, P., Loeloff, R., Luo, Y., Fisher, S., Fuller, J., Edenson, S., Lile, J., Jarosinski, M.A., Biere, A.L., Curran, E., Burgess, T., Louis, J.C., Collins, F., Treanor, J., Rogers, G., and Citron, M., Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science, 1999. 286(5440): pp. 735-41. 22. Sinha, S., Anderson, J.P., Barbour, R., Basi, G.S., Caccavello, R., Davis, D., Doan, M., Dovey, H.F., Frigon, N., Hong, J., Jacobson-Croak, K., Jewett, N., Keim, P., Knops, J., Lieberburg, I., Power, M., Tan, H., Tatsuno, G., Tung, J., Schenk, D., Seubert, P., Suomensaari, S.M., Wang, S., Walker, D., Zhao, J., McConlogue, L., and John, V., Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature, 1999. 402(6761): pp. 537-40. 23. Cook, D.G., Forman, M.S., Sung, J.C., Leight, S., Kolson, D.L., Iwatsubo, T., Lee, V.M., and Doms, R.W., Alzheimer's A beta(1-42) is generated in the endoplasmic reticulum/intermediate compartment of NT2N cells. Nat Med, 1997. 3(9): pp. 1021-3. 24. Hussain, I., Powell, D., Howlett, D.R., Tew, D.G., Meek, T.D., Chapman, C., Gloger, I.S., Murphy, K.E., Southan, C.D., Ryan, D.M., Smith, T.S., Simmons, D.L., Walsh, F.S., Dingwall, C., and Christie, G., Identification of a novel aspartic protease (Asp 2) as beta-secretase. Mol Cell Neurosci, 1999. 14(6): pp. 419-27. 25. Bennett, B.D., Denis, P., Haniu, M., Teplow, D.B., Kahn, S., Louis, J.C., Citron, M., and Vassar, R., A furin-like convertase mediates propeptide cleavage of BACE, the Alzheimer's beta -secretase. J Biol Chem, 2000. 275(48): pp. 37712-7. 26. Vassar, R., The beta-secretase, BACE: a prime drug target for Alzheimer's disease. J Mol Neurosci, 2001. 17(2): pp. 157-70. 27. Haniu, M., Denis, P., Young, Y., Mendiaz, E.A., Fuller, J., Hui, J.O., Bennett, B.D., Kahn, S., Ross, S., Burgess, T., Katta, V., Rogers, G., Vassar, R., and Citron, M., Characterization of Alzheimer's beta -secretase protein BACE. A pepsin family member with unusual properties. J Biol Chem, 2000. 275(28): pp. 21099-106. 28. Davies, P. and Maloney, A.J., Selective loss of central cholinergic neurons in Alzheimer's disease. Lancet, 1976. 2(8000): pp. 1403. 29. Blusztajn, J.K. and Berse, B., The cholinergic neuronal phenotype in Alzheimer's disease. Metab Brain Dis, 2000. 15(1): pp. 45-64. 30. Nordberg, A., Alafuzoff, I., and Winblad, B., Nicotinic and muscarinic subtypes in the human brain: changes with aging and dementia. J Neurosci Res, 1992. 31(1): pp. 103-11. 31. Francis, P.T., Palmer, A.M., Snape, M., and Wilcock, G.K., The cholinergic hypothesis of Alzheimer's disease: a review of progress. J Neurol Neurosurg Psychiatry, 1999. 66(2): pp. 137-47 32. Bartus, R.T., Dean, R.L., 3rd, Beer, B., and Lippa, A.S., The cholinergic hypothesis of geriatric memory dysfunction. Science, 1982. 217(4558): pp. 408-14. 33. Scarpini, E., Scheltens, P., and Feldman, H., Treatment of Alzheimer's disease: current status and new perspectives. Lancet Neurol, 2003. 2(9): pp. 539-47. 34. Solomon, B., Koppel, R., Hanan, E., and Katzav, T., Monoclonal antibodies inhibit in vitro fibrillar aggregation of the Alzheimer beta-amyloid peptide. Proc Natl Acad Sci U S A, 1996. 93(1): pp. 452-5. 35. Roberds, S.L., Anderson, J., Basi, G., Bienkowski, M.J., Branstetter, D.G., Chen, K.S., Freedman, S.B., Frigon, N.L., Games, D., Hu, K., Johnson-Wood, K., Kappenman, K.E., Kawabe, T.T., Kola, I., Kuehn, R., Lee, M., Liu, W., Motter, R., Nichols, N.F., Power, M., Robertson, D.W., Schenk, D., Schoor, M., Shopp, G.M., Shuck, M.E., Sinha, S., Svensson, K.A., Tatsuno, G., Tintrup, H., Wijsman, J., Wright, S., and McConlogue, L., BACE knockout mice are healthy despite lacking the primary beta-secretase activity in brain: implications for Alzheimer's disease therapeutics. Hum Mol Genet, 2001. 10(12): pp. 1317-24. 36. Luo, Y., Bolon, B., Kahn, S., Bennett, B.D., Babu-Khan, S., Denis, P., Fan, W., Kha, H., Zhang, J., Gong, Y., Martin, L., Louis, J.C., Yan, Q., Richards, W.G., Citron, M., and Vassar, R., Mice deficient in BACE1, the Alzheimer's beta-secretase, have normal phenotype and abolished beta-amyloid generation. Nat Neurosci, 2001. 4(3): pp. 231-2. 37. Hong, L., Koelsch, G., Lin, X., Wu, S., Terzyan, S., Ghosh, A.K., Zhang, X.C., and Tang, J., Structure of the protease domain of memapsin 2 (beta-secretase) complexed with inhibitor. Science, 2000. 290(5489): pp. 150-3. 38. Tung, J.S., Davis, D.L., Anderson, J.P., Walker, D.E., Mamo, S., Jewett, N., Hom, R.K., Sinha, S., Thorsett, E.D., and John, V., Design of substrate-based inhibitors of human beta-secretase. J Med Chem, 2002. 45(2): pp. 259-62. 39. Hu, J., Cwi, C.L., Smiley, D.L., Timm, D., Erickson, J.A., McGee, J.E., Yang, H.C., Mendel, D., May, P.C., Shapiro, M., and McCarthy, J.R., Design and synthesis of statine-containing BACE inhibitors. Bioorg Med Chem Lett, 2003. 13(24): pp. 4335-9. 40. Derossi, D., Chassaing, G., and Prochiantz, A., Trojan peptides: the penetratin system for intracellular delivery. Trends Cell Biol, 1998. 8(2): pp. 84-7. 41. Andrau, D., Dumanchin-Njock, C., Ayral, E., Vizzavona, J., Farzan, M., Boisbrun, M., Fulcrand, P., Hernandez, J.F., Martinez, J., Lefranc-Jullien, S., and Checler, F., BACE1- and BACE2-expressing human cells: characterization of beta-amyloid precursor protein-derived catabolites, design of a novel fluorimetric assay, and identification of new in vitro inhibitors. J Biol Chem, 2003. 278(28): pp. 25859-66. 42. Lefranc-Jullien S., Lisowski V., Hernandez J.-F., Martinez J., and Checler F., Design and characterization of a new cell-permeant inhibitor of the b-secretase BACE1. Br J Pharmacol, 2005. 145(2): pp. 228-35. 43. Qiao, L. and Etcheberrigaray, R. Patent WO 02/96897, 2002. 44. Bhisetti, G. R., Saunders, J. O., Murko, M. A., Lepre, C. A., Britt, S. D., Come, J. H.,Deninger, D. D., and Wang, T. Patent WO 02/88101, 2002. 45. Ermolieff, J., Loy, J.A., Koelsch, G., and Tang, J., Proteolytic activation of recombinant pro-memapsin 2 (pro-beta-secretase) studied with new fluorogenic substrates. Biochemistry, 2000. 39(40): pp. 12450-6. 46. Forster, T., Energiewanderung und Fluoreszenz. Naturwissenschaften, 1946 : pp. 166-75. 47. Forster, T., Zwischenmolekulare Energiewanderung und Fluoreszenz. Ann. Phys. (Leipzig), 1648: pp. 55-75. 48. Benjannet, S., Elagoz, A., Wickham, L., Mamarbachi, M., Munzer, J.S., Basak, A., Lazure, C., Cromlish, J.A., Sisodia, S., Checler, F., Chretien, M., and Seidah, N.G., Post-translational processing of beta-secretase (beta-amyloid-converting enzyme) and its ectodomain shedding. The pro- and transmembrane/cytosolic domains affect its cellular activity and amyloid-beta production. J Biol Chem, 2001. 276(14): pp. 10879-87. 49. Huse, J.T., Pijak, D.S., Leslie, G.J., Lee, V.M., and Doms, R.W., Maturation and endosomal targeting of beta-site amyloid precursor protein-cleaving enzyme. The Alzheimer's disease beta-secretase. J Biol Chem, 2000. 275(43): pp. 33729-37. 50. Sardana, V., Xu, B., Zugay-Murphy, J., Chen, Z., Sardana, M., Darke, P.L., Munshi, S., and Kuo, L.C., A general procedure for the purification of human beta-secretase expressed in Escherichia coli. Protein Expr Purif, 2004. 34(2): pp. 190-6. 51. Tian, G., Sobotka-Briner, C.D., Zysk, J., Liu, X., Birr, C., Sylvester, M.A., Edwards, P.D., Scott, C.D., and Greenberg, B.D., Linear non-competitive inhibition of solubilized human gamma-secretase by pepstatin A methylester, L685458, sulfonamides, and benzodiazepines. J Biol Chem, 2002. 277(35): pp. 31499-505. 52. Turner, R.T., 3rd, Loy, J.A., Nguyen, C., Devasamudram, T., Ghosh, A.K., Koelsch, G., and Tang, J., Specificity of memapsin 1 and its implications on the design of memapsin 2 (beta-secretase) inhibitor selectivity. Biochemistry, 2002. 41(27): pp. 8742-6. 53. Knops, J., Johnson-Wood, K., Schenk, D.B., Sinha, S., Lieberburg, I., and McConlogue, L., Isolation of baculovirus-derived secreted and full-length beta-amyloid precursor protein. J Biol Chem, 1991. 266(11): pp. 7285-90. 54. Roch, J.M., Shapiro, I.P., Sundsmo, M.P., Otero, D.A., Refolo, L.M., Robakis, N.K., and Saitoh, T., Bacterial expression, purification, and functional mapping of the amyloid beta/A4 protein precursor. J Biol Chem, 1992. 267(4): pp. 2214-21. 55. Chong, Y.H., Jung, J.M., Choi, W., Park, C.W., Choi, K.S., and Suh, Y.H., Bacterial expression, purification of full length and carboxyl terminal fragment of Alzheimer amyloid precursor protein and their proteolytic processing by thrombin. Life Sci, 1994. 54(17): pp. 1259-68. 56. Mallender, W.D., Yager, D., Onstead, L., Nichols, M.R., Eckman, C., Sambamurti, K., Kopcho, L.M., Marcinkeviciene, J., Copeland, R.A., and Rosenberry, T.L., Characterization of recombinant, soluble beta-secretase from an insect cell expression system. Mol Pharmacol, 2001. 59(3): pp. 619-26. 57. Huang, C. Y., Establishment of assay system for b-site APP cleaving enzyme activity and evaluation of inhibitors. 58. Hom, R.K., Gailunas, A.F., Mamo, S., Fang, L.Y., Tung, J.S., Walker, D.E., Davis, D., Thorsett, E.D., Jewett, N.E., Moon, J.B., and John, V., Design and synthesis of hydroxyethylene-based peptidomimetic inhibitors of human beta-secretase. J Med Chem, 2004. 47(1): pp. 158-64. 59. Teilum, K., Ostergaard, L., and Welinder, K.G., Disulfide bond formation and folding of plant peroxidases expressed as inclusion body protein in Escherichia coli thioredoxin reductase negative strains. Protein Expr Purif, 1999. 15(1): pp. 77-82. 60. Hong, L. and Tang, J., Flap position of free memapsin 2 (beta-secretase), a model for flap opening in aspartic protease catalysis. Biochemistry, 2004. 43(16): pp. 4689-95. 61. Lin, X.L., Lin, Y.Z., and Tang, J., Relationships of human immunodeficiency virus protease with eukaryotic aspartic proteases. Methods Enzymol, 1994. 241: pp. 195-224. 62. Luccio, E.D., Azulay D.O., Regaya I., Fajloun, Z., Sandoz, G., Mansuelle, P., Kharrat, R., Fathallah, M., Carrega, L., Esteave, E., Rochat, H., Waard, M.D. and Sabatier J.M. Biochem J, 2001. 358(Pt 3): pp. 681-92 63. Itoh, R.E., Kurokawa, K., Fujioka, A., Sharma, A., Mayer, B.J., and Matsuda, M., A FRET-based probe for epidermal growth factor receptor bound non-covalently to a pair of synthetic amphipathic helixes. Exp Cell Res, 2005. 307(1): pp. 142-52. 64. Centonze, V.E., Firulli, B.A., and Firulli, A.B., Fluorescence Resonance Energy Transfer (FRET) as a method to calculate the dimerization strength of basic Helix-Loop-Helix (bHLH) proteins. Biol Proced Online, 2004. 6: pp. 78-82. 65. Bruinzeel, W., Yon, J., Giovannelli, S., and Masure, S., Recombinant insect cell expression and purification of human beta-secretase (BACE-1) for X-ray crystallography. Protein Expr Purif, 2002. 26(1): pp. 139-48. 66. Sayle, R. and Bissell, A., RasMol: A Program for Fast Realistic Rendering of Molecular Structures with Shadows. Proceedings of the 10th Eurographics UK '92 Conference, 1992. 67. http://www.iba-go.com/iba_us/naps/naps_p_rg_fret.html | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35652 | - |
| dc.description.abstract | 阿滋海默症(Alzheimer’s disease, AD)是一種慢性神經退化疾病，在罹患阿滋海默症的病人腦中常可看到老年斑(senile plaque，SP)和神經纖維糾結(neurofibrillary tangles，NFT)這兩種病徵，特別以海馬迴(hippocampus)和杏仁核(amygdala)中最多。老年斑是阿滋海默症患者腦中常見的病徵，老年斑主要是由β-amyloid peptide (Aβ)大小約4 kDa的蛋白質所組成，Aβ是由amyloid precursor protein (APP)經由β-secretase及γ-secretase依序切割而形成。在之前的研究結果指出β-site APP cleaving enzyme (BACE)所反應為Aβ形成的速率決定步驟，所以BACE被認為是治療AD的一個重要的標的物。為了要成功建立一個研究in vitro酵素動力學系統及篩選具有BACE抑制活性的化合物，進而探討化合物對於BACE的抑制機轉，我們必須大量純化出BACE及BACE的受質APP695，於是建構了分別可以表現出具有glutathione-S-transferase (GST) APP融合蛋白及不具有transmembrane domain的BACE1-454與GST之融合蛋白的質體，將這些質體放入大腸桿菌中，利用IPTG (isopropyl-beta-D-thiogalactopyranoside)誘導的方式大量表現GST-APP695及GST-BACE1-454融合蛋白質，以便用親和性層析法純化這些蛋白質。由於經過誘導而大量表現的GST-BACE1-454融合蛋白質，在大腸桿菌內會以inclusion bodies的形式存在，於是我們運用denaturating及refolding的動作得到具有酵素活性之BACE1-454。同時，並利用實驗室之前利用FRET原理所建立篩選系統對一系列化合物之BACE抑制活性進行評估。 | zh_TW |
| dc.description.abstract | β-site APP cleaving enzyme (BACE) participates in the rate-limiting step about Aβ production. It is an important target for BACE to treat AD. In order to characterize BACE catalyzed reaction by in vitro enzyme kinetic studies, establish a screening system to identity potential BACE inhibitors, and determine the inhibitory mechanism of potential BACE inhibitors, it is necessary to obtain large amounts of BACE and its substrate, APP695. Plasmids encoding the glutathione-S-transferase (GST)-APP fusion protein and GST-BACE (without the transmembrane domain) fusion protein were constructed and transformed into specific Escherichia coli strains to induce overexpression of the above fusion proteins. Protein purification was accomplished by affinity chromatography. Since the GST-BACE1-454 fusion protein overexpressed in Escherichia coli was found to form inclusion bodies, a denaturating/refolding approach was applied to obtain active BACE1-454. In the mean while, we used the fluorescence resonance energy transfer (FRET) assay system previously established in our laboratory to screen a series of compounds for potential BACE inhibitors. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T07:03:09Z (GMT). No. of bitstreams: 1 ntu-94-R92423003-1.pdf: 1342987 bytes, checksum: ce2c02320e63ca907e4e6304d96e5d62 (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 目錄
中文摘要………………………………………………………………………………1 英文摘要………………………………………………………………………………2 英文縮寫表………………………………………………………………………3 第一章緒論…………………………………….….…………………………………..4 第二章 實驗動機與目的………………………..…………………………………..13 第三章 材料與方法………………………………………………….…...………....15 一、實驗材料…………………………………………………………………..15 二、實驗方法……………………………………………………………………15 建構質體pGEX4T-1-BACE(1-454)…………………………………………15 大腸桿菌系統的表現及純化蛋白質………………………………………..20 BACE抑制活性的評估……………………………………………………...25 抑制劑活性評估……………………………………………………………..25 第四章 結果…………………………………………………………………….….27 建構質體pGEX4T-1-BACE(1-454)………..………………………………..27 表現與純化GST-APP695融合蛋白…………………………………………..28 表現與純化GST-BACE1-454融合蛋白…..…………………………………...30 BACE活性的評估……………………………………………………….…..32 抑制劑活性評估……………………………………………………………..33 第五章 討論………………………………………………………………………....34 附圖…..………………………………………………………………………………48 附表……..……………………………………………………………………………77 參考資料………..……………………………………………………………………79 | |
| dc.language.iso | zh-TW | |
| dc.subject | 螢光共振能量傳遞系統 | zh_TW |
| dc.subject | 阿滋海默症 | zh_TW |
| dc.subject | BACE | zh_TW |
| dc.subject | APP | zh_TW |
| dc.subject | 蛋白質純化 | zh_TW |
| dc.subject | BACE | en |
| dc.subject | protein purification | en |
| dc.subject | FRET | en |
| dc.subject | APP | en |
| dc.title | 人類APP695及BACE1-454蛋白質之純化與BACE抑制劑的活性評估 | zh_TW |
| dc.title | Purification of Human APP695 and BACE1-454 and Activity Evaluation of BACE inhibitors | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳基旺,顧記華,忻凌偉 | |
| dc.subject.keyword | 阿滋海默症,BACE,APP,蛋白質純化,螢光共振能量傳遞系統, | zh_TW |
| dc.subject.keyword | BACE,APP,FRET,protein purification, | en |
| dc.relation.page | 87 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-07-27 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 藥學研究所 | zh_TW |
| 顯示於系所單位： | 藥學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-94-1.pdf 未授權公開取用 | 1.31 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。